##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548572_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	748184
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.724527656298452	31.0	31.0	34.0	30.0	34.0
2	31.917676667771563	33.0	31.0	34.0	30.0	34.0
3	32.093515766175166	34.0	31.0	34.0	30.0	34.0
4	35.80524175871176	37.0	35.0	37.0	35.0	37.0
5	35.558049891470546	37.0	35.0	37.0	33.0	37.0
6	34.19394694353261	37.0	35.0	37.0	32.0	37.0
7	34.9898300418079	37.0	35.0	37.0	32.0	37.0
8	35.66462661591266	37.0	35.0	37.0	33.0	37.0
9	37.34934454626135	39.0	37.0	39.0	34.0	39.0
10	36.77808801043594	39.0	37.0	39.0	32.0	39.0
11	36.76476642109428	39.0	37.0	39.0	32.0	39.0
12	36.70950595040792	39.0	35.0	39.0	32.0	39.0
13	36.66972696555928	39.0	35.0	39.0	32.0	39.0
14	37.828328325652514	40.0	37.0	41.0	33.0	41.0
15	37.83573425788309	40.0	37.0	41.0	33.0	41.0
16	37.77365327245704	40.0	37.0	41.0	33.0	41.0
17	37.74030853372967	39.0	37.0	41.0	33.0	41.0
18	37.60974439442704	39.0	36.0	41.0	33.0	41.0
19	37.562747933663374	39.0	36.0	41.0	32.0	41.0
20	37.45191824470986	39.0	36.0	41.0	32.0	41.0
21	37.415965858665786	39.0	36.0	41.0	32.0	41.0
22	37.39226446970264	39.0	36.0	41.0	32.0	41.0
23	37.25290302920137	39.0	35.0	41.0	32.0	41.0
24	37.0638759984175	39.0	35.0	40.0	31.0	41.0
25	37.133042139366786	39.0	35.0	40.0	32.0	41.0
26	37.06638874929162	39.0	35.0	40.0	31.0	41.0
27	37.02355436630562	39.0	35.0	40.0	31.0	41.0
28	36.955857115361994	39.0	35.0	40.0	31.0	41.0
29	36.78437790703891	39.0	35.0	40.0	31.0	41.0
30	36.764531184842234	39.0	35.0	40.0	31.0	41.0
31	36.705509607262385	39.0	35.0	40.0	31.0	41.0
32	36.61352421329512	39.0	35.0	40.0	31.0	41.0
33	36.521262149417794	39.0	35.0	40.0	30.0	41.0
34	36.41671032794072	39.0	35.0	40.0	30.0	41.0
35	36.28656587149685	38.0	35.0	40.0	30.0	41.0
36	36.215729018530205	38.0	35.0	40.0	30.0	41.0
37	36.16239454465747	38.0	35.0	40.0	30.0	41.0
38	36.06068026047068	38.0	35.0	40.0	30.0	41.0
39	35.96476267869936	38.0	35.0	40.0	30.0	41.0
40	35.82484121552987	38.0	35.0	40.0	29.0	41.0
41	35.698411086043	38.0	35.0	40.0	29.0	41.0
42	35.62289891256696	38.0	34.0	40.0	28.0	41.0
43	35.54863643167991	38.0	34.0	40.0	28.0	41.0
44	35.3904520813062	38.0	34.0	40.0	27.0	41.0
45	35.21025977567015	38.0	34.0	40.0	27.0	41.0
46	35.00832549212493	37.0	34.0	40.0	26.0	41.0
47	34.9164002437903	37.0	34.0	40.0	26.0	41.0
48	34.78920559648429	37.0	33.0	40.0	26.0	41.0
49	34.616040973878086	37.0	33.0	40.0	25.0	41.0
50	34.54348930209681	37.0	33.0	40.0	25.0	41.0
51	33.30104493012414	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	3.0
11	10.0
12	9.0
13	16.0
14	30.0
15	57.0
16	113.0
17	214.0
18	471.0
19	791.0
20	1308.0
21	1975.0
22	2806.0
23	3511.0
24	4643.0
25	5453.0
26	6628.0
27	7924.0
28	9713.0
29	12399.0
30	15793.0
31	20272.0
32	26363.0
33	35456.0
34	52414.0
35	69248.0
36	76405.0
37	102983.0
38	144301.0
39	146848.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.325925173486734	32.829892112100765	19.270126065245982	25.574056649166515
2	23.969237513766668	31.961790147878062	18.312072966008362	25.756899372346908
3	21.192781454829294	32.90580926616982	19.744741935138947	26.15666734386194
4	20.450718005196585	32.97851865316554	19.051864247297456	27.518899094340433
5	19.247404381809822	35.264320006843235	16.291179709804005	29.197095901542937
6	19.60694160794671	41.40264961560258	17.318734428963996	21.671674347486714
7	84.4089154539525	8.14144648909894	4.0654437945745965	3.3841942623739616
8	85.19147696288614	8.041738395902613	3.563561904558237	3.2032227366530157
9	78.6985554355613	11.260331683115384	5.744709857468217	4.2964030238550945
10	35.101525827871214	35.59792778247062	13.568988377190639	15.731558012467522
11	25.429707130866202	31.199410840114194	24.742844006287225	18.628038022732376
12	26.622060883419053	26.827758946996994	28.05419522470408	18.49598494487987
13	25.188189001635962	27.375217860847062	26.70038386279311	20.736209274723866
14	21.043486628957584	30.088454177047357	27.970659623835846	20.897399570159212
15	20.441896645744897	31.920356489847418	27.423200709985778	20.214546154421907
16	22.891427777124342	29.975246730750726	26.53317900409525	20.600146488029683
17	22.854003827935372	29.83610983394459	25.594105193374894	21.71578114474514
18	23.83558055237749	29.53083733413171	26.31531815703089	20.31826395645991
19	23.253504485527625	31.589689167370594	26.04452915325642	19.112277193845365
20	24.32930936774911	30.383034119949105	25.442805513082345	19.844850999219442
21	24.862066015846366	29.57307293393069	25.920896463971427	19.64396458625151
22	23.081220662296975	27.717112368080578	26.878682249286275	22.322984720336176
23	21.682634218320626	30.45520887909926	26.012585139484408	21.84957176309571
24	20.54053548325011	31.400163596120738	27.054173839590263	21.00512708103889
25	22.120227109908793	30.67868331854196	25.595842733872953	21.605246837676294
26	22.536434887674687	30.029912427958898	25.537701955668656	21.895950728697752
27	20.151460068646216	29.9656234295307	27.12394277343541	22.75897372838767
28	21.45180864600152	30.062658383499247	27.363456048244817	21.12207692225442
29	22.364819349250986	30.70006843236423	24.566817788137676	22.368294430247104
30	21.893945874276916	29.27247842776643	27.501390032398447	21.332185665558203
31	24.15248120783123	30.536739625546655	23.163553350512707	22.147225816109405
32	23.253237171604844	28.592832779102466	26.502838873859908	21.651091175432782
33	21.963848465083455	28.90051110422035	26.325208772173692	22.810431658522504
34	22.02947403312554	28.838494274135773	28.07023406007079	21.0617976326679
35	22.12744458582381	29.32487195663099	26.965826588112012	21.58185686943319
36	23.013590239834052	30.95575419950173	25.499342407749964	20.531313152914258
37	23.199908044010563	30.897479764336044	24.721191578542175	21.181420613111214
38	23.358692514140905	29.185868716786246	26.83791687606257	20.617521893010277
39	22.60125851394844	28.176357687413788	26.0879676657079	23.134416132929868
40	23.271013547469607	29.151919848593394	26.100130449194314	21.476936154742685
41	21.392331298183333	27.420260256835217	28.374036333308382	22.813372111673065
42	22.158853971750265	28.31602921206548	27.13222950504154	22.392887311142715
43	21.585866578274864	29.0357719491462	26.53812431166665	22.840237160912288
44	22.733578905723725	28.00273729456925	26.823882895116707	22.439800904590314
45	23.647391550741528	28.12971140788897	26.88763726569935	21.335259775670156
46	21.85424975674433	28.211776782181925	27.11739358232734	22.816579878746403
47	21.598296675684058	28.813901393240165	28.289297819787645	21.298504111288132
48	22.215925494263445	27.94807159736108	27.653491654459334	22.182511253916147
49	21.79878211776782	28.39555510409204	27.608983886316736	22.196678891823403
50	21.981758497909606	27.839007516867508	27.897014638110413	22.282219347112473
51	21.822439399933707	27.291147632133274	28.514910770612577	22.371502197320446
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15620.0
1	11683.5
2	7747.0
3	4882.5
4	2018.0
5	1656.0
6	1294.0
7	1441.5
8	1589.0
9	1937.5
10	2286.0
11	2431.0
12	2576.0
13	2520.0
14	2464.0
15	2577.0
16	2690.0
17	2840.0
18	2990.0
19	2762.0
20	2534.0
21	2991.0
22	3448.0
23	3081.5
24	2715.0
25	4487.0
26	7305.5
27	8352.0
28	9402.5
29	10453.0
30	13665.5
31	16878.0
32	17582.5
33	18287.0
34	20459.5
35	22632.0
36	24141.5
37	25651.0
38	27378.5
39	29106.0
40	31656.5
41	34207.0
42	36048.0
43	37889.0
44	40713.0
45	43537.0
46	47171.5
47	50806.0
48	52547.0
49	54288.0
50	56029.5
51	57771.0
52	52361.0
53	46951.0
54	43063.0
55	39175.0
56	36798.0
57	34421.0
58	33617.5
59	32814.0
60	30216.0
61	27618.0
62	25690.5
63	23763.0
64	21790.0
65	19817.0
66	18601.5
67	17386.0
68	14602.5
69	11819.0
70	10753.5
71	9688.0
72	7901.0
73	6114.0
74	5671.5
75	4037.0
76	2845.0
77	2449.5
78	2054.0
79	1470.0
80	886.0
81	844.0
82	802.0
83	534.5
84	267.0
85	233.5
86	200.0
87	134.5
88	69.0
89	59.0
90	49.0
91	64.0
92	79.0
93	61.5
94	44.0
95	23.0
96	2.0
97	3.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	748184.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.22624029140924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.85560539247392	17.304373934862625
2	8.791665068552138	3.9081132075045737
3	3.206899921905299	2.138319847643061
4	1.6797725785734647	1.4934011586517577
5	1.0403732983614793	1.1561793461074121
6	0.7287981667933005	0.9719065907451868
7	0.5303706044219343	0.8251701148167309
8	0.4162858960818537	0.7401976284991918
9	0.34401208784463827	0.6881485794825882
>10	3.365251501431462	17.157349110278883
>50	1.1297551353081905	17.981392407102962
>100	0.9045325908700719	32.19261037632026
>500	0.003642413117597605	0.5231181123642803
>1k	0.002428275411731737	1.0991282288675335
>5k	0.0	0.0
>10k+	6.070688529329342E-4	1.8205913567529621
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13493	1.803433380024165	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2913	0.38934272852667257	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2437	0.32572201490542435	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1442	0.19273333832319323	No Hit
CTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGCT	1354	0.18097152572094566	TruSeq Adapter, Index 1 (96% over 27bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	800	0.10692556911134159	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.23336505458550302	0.0
2	0.0	0.0	0.0	0.3211776782181923	0.0
3	0.0	0.0	0.0	0.42970713086620405	0.0
4	0.0	0.0	0.0	0.9552463030484479	0.0
5	0.0	0.0	0.0	1.0530831987853255	0.0
6	0.0	0.0	0.0	1.5742116912417266	0.0
7	0.0	0.0	0.0	1.9562032868919945	0.0
8	0.0	0.0	0.0	2.281925301797419	0.0
9	0.0	0.0	0.0	2.8027864803310414	0.0
10	0.0	0.0	0.0	3.6433551105075757	0.0
11	0.0	0.0	0.0	4.3835473626808374	0.0
12	0.0	0.0	0.0	4.730119863562974	0.0
13	0.0	0.0	0.0	4.8802166312030195	0.0
14	0.0	0.0	0.0	5.041273269676978	0.0
15	0.0	0.0	0.0	5.175999486757268	0.0
16	0.0	0.0	0.0	5.449194315836746	0.0
17	0.0	0.0	0.0	5.790019567379147	0.0
18	0.0	0.0	0.0	6.269447087882125	0.0
19	0.0	0.0	0.0	6.448814730066401	0.0
20	0.0	0.0	0.0	6.683650011227185	0.0
21	0.0	0.0	0.0	6.904317654480716	0.0
22	0.0	0.0	0.0	7.14396458625151	0.0
23	0.0	0.0	0.0	7.377329640837013	0.0
24	0.0	0.0	0.0	7.560840648824353	0.0
25	0.0	0.0	0.0	7.72550602525582	0.0
26	0.0	0.0	0.0	7.8666477764827905	0.0
27	0.0	0.0	0.0	8.044545192091785	0.0
28	0.0	0.0	0.0	8.217229986206602	0.0
29	0.0	0.0	0.0	8.39125134993531	0.0
30	0.0	0.0	0.0	8.628091485516931	0.0
31	0.0	0.0	0.0	8.790885664488949	0.0
32	0.0	0.0	0.0	8.958758807993755	0.0
33	0.0	0.0	0.0	9.154165285544732	0.0
34	0.0	0.0	0.0	9.326315451813992	0.0
35	0.0	0.0	0.0	9.533884712851384	0.0
36	0.0	0.0	0.0	9.701356885472023	0.0
37	0.0	0.0	0.0	9.887541032687146	0.0
38	0.0	0.0	0.0	10.075596377361718	0.0
39	0.0	0.0	0.0	10.290784085198293	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGA	35	1.2111741E-7	45.000004	37
AATCCCG	35	1.2111741E-7	45.000004	1
CGTATAA	30	2.164481E-6	45.000004	21
TTAGACG	30	2.164481E-6	45.000004	45
ATTACGT	35	1.2111741E-7	45.000004	36
TTGTCGT	30	2.164481E-6	45.000004	39
TAAGTCG	30	2.164481E-6	45.000004	1
CGATTCG	35	1.2111741E-7	45.000004	40
TCAATCG	35	1.2111741E-7	45.000004	40
CGCTAAC	35	1.2111741E-7	45.000004	35
TCGTCGT	35	1.2111741E-7	45.000004	39
CGAGCAT	35	1.2111741E-7	45.000004	42
TTACGTG	35	1.2111741E-7	45.000004	37
CGGCTAT	35	1.2111741E-7	45.000004	42
ATAGTCG	30	2.164481E-6	45.000004	1
ATACGTA	30	2.164481E-6	45.000004	18
CGATATT	30	2.164481E-6	45.000004	31
GTACGAG	35	1.2111741E-7	45.000004	40
TACCGAA	30	2.164481E-6	45.000004	24
CAGGTAC	20	7.0317055E-4	45.0	21
>>END_MODULE