##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548571_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	743245
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70925872357029	31.0	31.0	34.0	30.0	34.0
2	31.906544948166488	33.0	31.0	34.0	30.0	34.0
3	32.07585520252407	33.0	31.0	34.0	30.0	34.0
4	35.80569260472657	37.0	35.0	37.0	35.0	37.0
5	35.577381617098	37.0	35.0	37.0	33.0	37.0
6	32.781948079031814	37.0	35.0	37.0	26.0	37.0
7	34.28696055809323	37.0	35.0	37.0	28.0	37.0
8	35.38514352602439	37.0	35.0	37.0	32.0	37.0
9	37.2374560205585	39.0	37.0	39.0	34.0	39.0
10	36.7432327159954	39.0	37.0	39.0	32.0	39.0
11	36.771462976542054	39.0	37.0	39.0	32.0	39.0
12	36.72908664034067	39.0	35.0	39.0	32.0	39.0
13	36.70176455946559	39.0	35.0	39.0	32.0	39.0
14	37.8361764963101	40.0	37.0	41.0	33.0	41.0
15	37.89381966915351	40.0	37.0	41.0	33.0	41.0
16	37.84227542734899	40.0	37.0	41.0	33.0	41.0
17	37.836617804357914	39.0	37.0	41.0	33.0	41.0
18	37.70080390719077	39.0	37.0	41.0	33.0	41.0
19	37.64996737280439	39.0	36.0	41.0	33.0	41.0
20	37.53256194121723	39.0	36.0	41.0	32.0	41.0
21	37.477532980376594	39.0	36.0	41.0	32.0	41.0
22	37.42666280970609	39.0	36.0	41.0	32.0	41.0
23	37.29106014840328	39.0	35.0	41.0	32.0	41.0
24	37.1538711999408	39.0	35.0	41.0	32.0	41.0
25	37.19837334929936	39.0	35.0	41.0	32.0	41.0
26	37.14429965892808	39.0	35.0	41.0	32.0	41.0
27	37.07384240728158	39.0	35.0	41.0	32.0	41.0
28	37.03478395414702	39.0	35.0	40.0	31.0	41.0
29	36.825364449138576	39.0	35.0	40.0	31.0	41.0
30	36.81666879696466	39.0	35.0	40.0	31.0	41.0
31	36.46273839716379	39.0	35.0	40.0	30.0	41.0
32	36.54940295595665	39.0	35.0	40.0	30.0	41.0
33	36.50277902979502	39.0	35.0	40.0	30.0	41.0
34	36.43343312097626	39.0	35.0	40.0	30.0	41.0
35	36.38473181790661	39.0	35.0	40.0	30.0	41.0
36	36.30277364798956	38.0	35.0	40.0	30.0	41.0
37	36.235176825945686	38.0	35.0	40.0	30.0	41.0
38	36.11468358347517	38.0	35.0	40.0	30.0	41.0
39	36.00609624013616	38.0	35.0	40.0	30.0	41.0
40	35.90344233731811	38.0	35.0	40.0	29.0	41.0
41	35.83708063962758	38.0	35.0	40.0	29.0	41.0
42	35.737932983067495	38.0	35.0	40.0	29.0	41.0
43	35.68559896131155	38.0	35.0	40.0	29.0	41.0
44	35.49273927170717	38.0	34.0	40.0	28.0	41.0
45	35.29736627895243	38.0	34.0	40.0	27.0	41.0
46	35.00729772820537	37.0	34.0	40.0	26.0	41.0
47	34.97001661632436	37.0	34.0	40.0	26.0	41.0
48	34.94562627397426	37.0	34.0	40.0	26.0	41.0
49	34.81429811165901	37.0	34.0	40.0	26.0	41.0
50	34.704704370698764	37.0	33.0	40.0	26.0	41.0
51	33.541941082684716	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	6.0
11	10.0
12	10.0
13	12.0
14	23.0
15	54.0
16	126.0
17	236.0
18	447.0
19	864.0
20	1307.0
21	1957.0
22	2632.0
23	3556.0
24	4397.0
25	5404.0
26	6161.0
27	7507.0
28	9377.0
29	12005.0
30	15461.0
31	19930.0
32	26257.0
33	35753.0
34	52447.0
35	69528.0
36	78410.0
37	106064.0
38	141534.0
39	141745.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.344583549166156	32.64764646919925	19.4074632187233	25.60030676291129
2	23.97432878795014	31.855310160175986	18.270153179637937	25.900207872235935
3	21.211578954449745	33.02571830284764	19.762931469434708	25.999771273267903
4	20.347933723065744	32.962482088678705	19.1009693977087	27.588614790546856
5	19.37019421590458	35.23266217734394	16.24740159705077	29.149742009700702
6	18.770795632664868	43.81179826302229	16.613095278138434	20.80431082617441
7	84.43635678679306	8.137289857314883	4.088423063727304	3.337930292164764
8	85.06508620979623	8.101635396134519	3.509206250967043	3.324072143102207
9	78.71078850177263	11.260082476168693	5.718571937920873	4.310557084137801
10	35.23723671198595	35.633203048792794	13.47469542344718	15.654864815774072
11	25.444638039946447	31.301118742810246	24.73195245174875	18.522290765494553
12	26.618678901304417	26.794260304475642	28.170657051174242	18.4164037430457
13	25.280762063653306	27.411822481146864	26.789551224697107	20.517864230502727
14	21.059812040444267	30.07366346225	27.943275770439087	20.923248726866646
15	20.47090797785387	31.90818639883215	27.380204374062387	20.240701249251593
16	22.81441516592779	30.019576317365065	26.578046270072452	20.58796224663469
17	22.924876723018656	29.796096845589275	25.66946296308754	21.60956346830453
18	23.781660152439642	29.487181212117136	26.320123243345062	20.411035392098164
19	23.302410376120932	31.621874348297	26.035156644175206	19.04055863140687
20	24.317149795827756	30.496000645816657	25.460245275783894	19.726604282571696
21	24.928791986491667	29.60208275871348	25.850695261993017	19.618429992801836
22	23.0570000470908	27.8177451580569	26.906201858068336	22.219052936783967
23	21.78447214579311	30.48173886134451	25.938553236146895	21.795235756715485
24	20.618503992626927	31.437682056387867	27.039939723778833	20.903874227206373
25	22.14989673660771	30.69284018055957	25.563845030911747	21.593418051920967
26	22.462714179039214	30.0183654111363	25.62358307153092	21.895337338293565
27	20.193341361193145	30.00302726557192	27.053663327704864	22.749968045530075
28	21.403305774004533	30.186412286661866	27.38679708575234	21.023484853581255
29	22.40889612442734	30.600273126627155	24.633196321535966	22.357634427409533
30	21.881344644094476	29.216072762009837	27.580003901808958	21.322578692086726
31	24.153677454944198	30.61466945623583	23.05592368599856	22.17572940282141
32	23.273483171767047	28.68811764626738	26.53768272911355	21.50071645285202
33	21.97579532993831	28.922091638692493	26.31339598651858	22.78871704485062
34	22.02705702695612	28.827640952848654	28.150475280694792	20.994826739500432
35	22.12756224394379	29.333530666200243	26.890863712503954	21.648043377352018
36	22.878189560642856	30.99785400507235	25.572725009922703	20.551231424362086
37	23.10220721296477	30.99893036616459	24.72831973306245	21.17054268780819
38	23.454984560945583	29.200465526172394	26.788474863604865	20.556075049277155
39	22.549899427510447	28.369649308101636	26.098258313207623	22.9821929511803
40	23.284246782689422	29.201138251855042	26.104985570034106	21.40962939542143
41	21.394156704720515	27.519189500097546	28.381758370389303	22.704895424792632
42	22.156623993434195	28.5218198575167	27.09577595543865	22.22578019361045
43	21.61830890217896	29.057713136314405	26.48399921963821	22.83997874186843
44	22.841458738370253	27.93883578093361	26.831663852430893	22.388041628265242
45	23.55266433006613	28.16796614844365	27.036845185638654	21.24252433585157
46	21.758370389306354	28.2765440736231	27.194801175924493	22.770284361146057
47	21.566643569751562	28.906349857718517	28.257169573962827	21.269836998567094
48	22.29776184165383	28.084413618658722	27.669072782191606	21.948751757495845
49	21.74989404570498	28.607256019213047	27.53708400325599	22.105765931825978
50	22.02207885690452	27.957672100047766	27.88824680959845	22.132002233449267
51	21.828602950574844	27.46227690734549	28.581961533545464	22.1271586085342
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15754.0
1	11791.0
2	7828.0
3	4941.5
4	2055.0
5	1665.5
6	1276.0
7	1445.0
8	1614.0
9	1939.0
10	2264.0
11	2448.0
12	2632.0
13	2494.5
14	2357.0
15	2486.5
16	2616.0
17	2751.5
18	2887.0
19	2709.5
20	2532.0
21	3005.0
22	3478.0
23	3090.0
24	2702.0
25	4494.5
26	7317.5
27	8348.0
28	9463.5
29	10579.0
30	13736.0
31	16893.0
32	17666.5
33	18440.0
34	20471.0
35	22502.0
36	24055.5
37	25609.0
38	27385.5
39	29162.0
40	31514.0
41	33866.0
42	35784.5
43	37703.0
44	40557.5
45	43412.0
46	46894.0
47	50376.0
48	52219.5
49	54063.0
50	55506.5
51	56950.0
52	51650.0
53	46350.0
54	42529.0
55	38708.0
56	36597.5
57	34487.0
58	33652.0
59	32817.0
60	30045.0
61	27273.0
62	25435.0
63	23597.0
64	21480.0
65	19363.0
66	18384.0
67	17405.0
68	14469.0
69	11533.0
70	10395.0
71	9257.0
72	7671.0
73	6085.0
74	5575.0
75	3914.5
76	2764.0
77	2404.5
78	2045.0
79	1468.5
80	892.0
81	829.0
82	766.0
83	503.5
84	241.0
85	214.0
86	187.0
87	117.0
88	47.0
89	51.0
90	55.0
91	68.5
92	82.0
93	59.0
94	36.0
95	20.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	743245.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.472447140129404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.2607473819265	17.362380615472343
2	8.714737872003594	3.9168297233736915
3	3.278854536835898	2.210516557776545
4	1.72223542996203	1.5481137865071901
5	1.087775709980462	1.2222491071426331
6	0.8295190322518634	1.1184793562406778
7	0.62521610015444	0.9835095033314952
8	0.437001525982382	0.7856394954235972
9	0.38364439709927534	0.7759285590978251
>10	3.7028128674454575	18.660456177577597
>50	1.107650871315835	17.969305366550522
>100	0.8425409906402418	29.910212650884077
>500	0.004236915901208112	0.5791723608772529
>1k	0.00242109480069035	1.1142743025614033
>5k	0.0	0.0
>10k+	6.052737001725875E-4	1.8429324371831608
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13549	1.8229520548405975	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2849	0.38331909397305064	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2555	0.3437628238333255	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1492	0.2007413437022785	No Hit
CTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGCT	1296	0.17437049694246176	TruSeq Adapter, Index 1 (96% over 27bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	832	0.11194155359269152	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21258131571689012	0.0
2	0.0	0.0	0.0	0.30030474473423974	0.0
3	0.0	0.0	0.0	0.3975808784451964	0.0
4	0.0	0.0	0.0	0.9048160431620798	0.0
5	0.0	0.0	0.0	0.99644128113879	0.0
6	0.0	0.0	0.0	1.4825528594205142	0.0
7	0.0	0.0	0.0	1.8490538113273551	0.0
8	0.0	0.0	0.0	2.165638517581686	0.0
9	0.0	0.0	0.0	2.670048234431446	0.0
10	0.0	0.0	0.0	3.499249910863847	0.0
11	0.0	0.0	0.0	4.195117356995338	0.0
12	0.0	0.0	0.0	4.535112917005833	0.0
13	0.0	0.0	0.0	4.67826894227341	0.0
14	0.0	0.0	0.0	4.822635873769753	0.0
15	0.0	0.0	0.0	4.953682836749659	0.0
16	0.0	0.0	0.0	5.232863995048739	0.0
17	0.0	0.0	0.0	5.54917961103001	0.0
18	0.0	0.0	0.0	6.011745790419041	0.0
19	0.0	0.0	0.0	6.195938082328169	0.0
20	0.0	0.0	0.0	6.417937557602136	0.0
21	0.0	0.0	0.0	6.630653418455556	0.0
22	0.0	0.0	0.0	6.873911025301213	0.0
23	0.0	0.0	0.0	7.118648628648696	0.0
24	0.0	0.0	0.0	7.291135493679742	0.0
25	0.0	0.0	0.0	7.4451896750062225	0.0
26	0.0	0.0	0.0	7.594131141144575	0.0
27	0.0	0.0	0.0	7.766618006175622	0.0
28	0.0	0.0	0.0	7.9342612462916	0.0
29	0.0	0.0	0.0	8.112937187603011	0.0
30	0.0	0.0	0.0	8.343951187024468	0.0
31	0.0	0.0	0.0	8.516976232601632	0.0
32	0.0	0.0	0.0	8.686234014355966	0.0
33	0.0	0.0	0.0	8.860066330752309	0.0
34	0.0	0.0	0.0	9.033225921466004	0.0
35	0.0	0.0	0.0	9.23881089008335	0.0
36	0.0	0.0	0.0	9.414661383527639	0.0
37	0.0	0.0	0.0	9.59360641511211	0.0
38	0.0	0.0	0.0	9.783449602755484	0.0
39	0.0	0.0	0.0	9.99818364065685	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAT	25	3.889471E-5	45.0	27
TCGTTCG	20	7.031676E-4	45.0	16
AATCCCG	35	1.2111559E-7	45.0	1
GTACCGC	25	3.889471E-5	45.0	26
CAAACGT	45	3.8380676E-10	45.0	22
CGAACAT	20	7.031676E-4	45.0	13
ATCTACG	20	7.031676E-4	45.0	1
CGAGTAC	25	3.889471E-5	45.0	10
GCGAATT	20	7.031676E-4	45.0	22
ACGTTGC	25	3.889471E-5	45.0	23
TTATGGC	20	7.031676E-4	45.0	20
CGCATAG	40	6.8102963E-9	45.0	1
GACCGAA	20	7.031676E-4	45.0	9
TAAGTCG	20	7.031676E-4	45.0	1
TAACGCA	20	7.031676E-4	45.0	29
GAGCGTA	25	3.889471E-5	45.0	19
CTACGAA	20	7.031676E-4	45.0	10
CGCTAAC	20	7.031676E-4	45.0	35
ACTACGT	25	3.889471E-5	45.0	22
TTGCGTA	35	1.2111559E-7	45.0	25
>>END_MODULE