Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548569_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 468257 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 15104 | 3.2255791157419966 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3436 | 0.7337850795610102 | No Hit |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2701 | 0.5768199941485125 | No Hit |
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT | 2202 | 0.47025458241948326 | Illumina PCR Primer Index 11 (95% over 24bp) |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1188 | 0.2537068319320373 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGT | 960 | 0.20501562176326246 | No Hit |
AACCCTGGGAACTTACATTGAAAAGCAGGCTATCCCTCCCTTCTAATCCTC | 814 | 0.17383616262009965 | No Hit |
AAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT | 804 | 0.17170058322673235 | Illumina PCR Primer Index 11 (95% over 21bp) |
AAAAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTT | 792 | 0.16913788795469153 | No Hit |
AATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT | 752 | 0.16059557038122227 | Illumina PCR Primer Index 11 (95% over 21bp) |
AATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCC | 618 | 0.13197880651010022 | No Hit |
GCAAGAGGGAAACACTAACTTTTTTTCTTTAGGAAGTGAATATTATTTTTT | 498 | 0.1063518537896924 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAATCGA | 45 | 3.8380676E-10 | 45.000004 | 42 |
TGTATCG | 45 | 3.8380676E-10 | 45.000004 | 1 |
CGGGCTA | 65 | 0.0 | 45.000004 | 6 |
CAAATCG | 45 | 3.8380676E-10 | 45.000004 | 41 |
ACGCTCG | 45 | 3.8380676E-10 | 45.000004 | 11 |
ACGTCCA | 65 | 0.0 | 45.000004 | 27 |
CATCGGG | 45 | 3.8380676E-10 | 45.000004 | 3 |
TAGTGCG | 65 | 0.0 | 45.000004 | 1 |
TCACGGT | 35 | 1.2098644E-7 | 45.0 | 12 |
TCCGGCT | 25 | 3.887253E-5 | 45.0 | 21 |
TCGCCTG | 25 | 3.887253E-5 | 45.0 | 30 |
TCGCCTA | 20 | 7.0289994E-4 | 45.0 | 32 |
GTTGATC | 35 | 1.2098644E-7 | 45.0 | 16 |
GTTGACG | 35 | 1.2098644E-7 | 45.0 | 12 |
CTATGTC | 25 | 3.887253E-5 | 45.0 | 11 |
CGGCGTA | 20 | 7.0289994E-4 | 45.0 | 18 |
TTAGGCA | 20 | 7.0289994E-4 | 45.0 | 44 |
CGTGGAC | 35 | 1.2098644E-7 | 45.0 | 17 |
TTAGGAG | 20 | 7.0289994E-4 | 45.0 | 44 |
AACCGCG | 35 | 1.2098644E-7 | 45.0 | 1 |