FastQCFastQC Report
Sat 18 Jun 2016
SRR3548569_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548569_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468257
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA151043.2255791157419966No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34360.7337850795610102No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27010.5768199941485125No Hit
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT22020.47025458241948326Illumina PCR Primer Index 11 (95% over 24bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11880.2537068319320373No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGT9600.20501562176326246No Hit
AACCCTGGGAACTTACATTGAAAAGCAGGCTATCCCTCCCTTCTAATCCTC8140.17383616262009965No Hit
AAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT8040.17170058322673235Illumina PCR Primer Index 11 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTT7920.16913788795469153No Hit
AATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT7520.16059557038122227Illumina PCR Primer Index 11 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCC6180.13197880651010022No Hit
GCAAGAGGGAAACACTAACTTTTTTTCTTTAGGAAGTGAATATTATTTTTT4980.1063518537896924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGA453.8380676E-1045.00000442
TGTATCG453.8380676E-1045.0000041
CGGGCTA650.045.0000046
CAAATCG453.8380676E-1045.00000441
ACGCTCG453.8380676E-1045.00000411
ACGTCCA650.045.00000427
CATCGGG453.8380676E-1045.0000043
TAGTGCG650.045.0000041
TCACGGT351.2098644E-745.012
TCCGGCT253.887253E-545.021
TCGCCTG253.887253E-545.030
TCGCCTA207.0289994E-445.032
GTTGATC351.2098644E-745.016
GTTGACG351.2098644E-745.012
CTATGTC253.887253E-545.011
CGGCGTA207.0289994E-445.018
TTAGGCA207.0289994E-445.044
CGTGGAC351.2098644E-745.017
TTAGGAG207.0289994E-445.044
AACCGCG351.2098644E-745.01