##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548569_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 468257 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.50704847978781 31.0 31.0 34.0 30.0 34.0 2 31.710797702970805 31.0 31.0 34.0 30.0 34.0 3 31.878032789686007 33.0 31.0 34.0 30.0 34.0 4 35.63633645626226 37.0 35.0 37.0 33.0 37.0 5 35.3940058557587 37.0 35.0 37.0 33.0 37.0 6 32.63159547000899 37.0 35.0 37.0 26.0 37.0 7 34.11113128047205 37.0 35.0 37.0 27.0 37.0 8 35.17939721136043 37.0 35.0 37.0 32.0 37.0 9 36.9914662247441 39.0 37.0 39.0 33.0 39.0 10 36.55624368669342 38.0 35.0 39.0 32.0 39.0 11 36.62766600392519 39.0 35.0 39.0 32.0 39.0 12 36.58405747271264 39.0 35.0 39.0 32.0 39.0 13 36.5817168776975 39.0 35.0 39.0 32.0 39.0 14 37.65171903463269 39.0 37.0 41.0 32.0 41.0 15 37.711846272452945 39.0 37.0 41.0 33.0 41.0 16 37.65752994616204 39.0 36.0 41.0 32.0 41.0 17 37.6087639907145 39.0 36.0 41.0 33.0 41.0 18 37.42381213735192 39.0 36.0 40.0 32.0 41.0 19 37.3503140369498 39.0 35.0 40.0 32.0 41.0 20 37.23421326322938 39.0 35.0 40.0 32.0 41.0 21 37.13786873447701 39.0 35.0 40.0 32.0 41.0 22 37.04935323978072 39.0 35.0 40.0 31.0 41.0 23 36.87506006317044 39.0 35.0 40.0 31.0 41.0 24 36.7682725511845 39.0 35.0 40.0 31.0 41.0 25 36.80203819697303 39.0 35.0 40.0 31.0 41.0 26 36.75539714302189 39.0 35.0 40.0 31.0 41.0 27 36.62113113098149 39.0 35.0 40.0 31.0 41.0 28 36.549621254994584 39.0 35.0 40.0 31.0 41.0 29 36.32610938010537 38.0 35.0 40.0 30.0 41.0 30 36.2835344693192 38.0 35.0 40.0 30.0 41.0 31 35.92342666527142 38.0 35.0 40.0 29.0 41.0 32 35.99198089937791 38.0 35.0 40.0 29.0 41.0 33 35.937192610041066 38.0 35.0 40.0 29.0 41.0 34 35.940045744110606 38.0 35.0 40.0 29.0 41.0 35 35.85929948724739 38.0 35.0 40.0 29.0 41.0 36 35.76329451561856 38.0 35.0 40.0 29.0 41.0 37 35.662999164988456 38.0 35.0 40.0 28.0 41.0 38 35.58387808404359 38.0 34.0 40.0 28.0 41.0 39 35.49877310963851 38.0 34.0 40.0 27.0 41.0 40 35.3798362864837 38.0 34.0 40.0 27.0 41.0 41 35.35120457355683 38.0 34.0 40.0 27.0 41.0 42 35.24448753569087 38.0 34.0 40.0 27.0 41.0 43 35.15623258168057 38.0 34.0 40.0 26.0 41.0 44 34.93208430413213 38.0 34.0 40.0 26.0 41.0 45 34.75628981520831 37.0 33.0 40.0 25.0 41.0 46 34.48255551972528 37.0 33.0 40.0 24.0 41.0 47 34.49140536073139 37.0 33.0 40.0 24.0 41.0 48 34.46642762414657 37.0 33.0 40.0 24.0 41.0 49 34.304097963297934 37.0 33.0 40.0 24.0 41.0 50 34.20201086155679 37.0 33.0 40.0 23.0 41.0 51 33.04494967507159 35.0 31.0 39.0 20.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 2.0 11 6.0 12 5.0 13 19.0 14 26.0 15 48.0 16 86.0 17 185.0 18 378.0 19 664.0 20 1131.0 21 1746.0 22 2285.0 23 2888.0 24 3692.0 25 4118.0 26 4756.0 27 5788.0 28 6859.0 29 8801.0 30 10898.0 31 13975.0 32 18339.0 33 24393.0 34 35545.0 35 48876.0 36 49035.0 37 63946.0 38 81642.0 39 78113.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.027982496791292 34.8240389358835 18.30383742261194 25.844141144713266 2 23.927672197105434 34.00974251319254 17.996313989967046 24.066271299734975 3 19.6291779941357 35.5339055262388 19.67936410987983 25.157552369745673 4 18.9024403265728 35.79316486459359 17.431880356300066 27.872514452533544 5 16.52724892526967 39.51163570432476 16.36921605016049 27.591899320245076 6 17.185648052244815 46.722846641908184 17.238396863260988 18.853108442586016 7 78.30635740629612 12.394689241164574 4.889409021114473 4.409544331424837 8 79.50548523567187 10.895726064960055 4.61648197464213 4.982306724725952 9 73.2527223298317 13.985268773344552 8.025293802335042 4.73671509448871 10 32.97078313831934 36.20575880339215 13.220090676701043 17.60336738158746 11 23.924468828015385 34.61218946006146 23.204992130389932 18.258349581533217 12 24.21170425642329 29.6792146193223 28.037381181701505 18.071699942552915 13 23.846733738096816 29.94765694906857 24.990336503245015 21.215272809589607 14 19.498907651140293 33.6234162009324 27.88981264561982 18.987863502307494 15 18.242119178143625 34.575457494495545 27.436215582468606 19.746207744892228 16 19.683421710727227 32.31772295982761 26.531584151438203 21.46727117800695 17 19.268478634595958 32.44735262900501 26.39661553377739 21.88755320262164 18 19.054493579380555 31.8769393730366 26.919832485152384 22.148734562430462 19 20.466111558396353 35.00043779377564 23.68699239947294 20.84645824835507 20 21.450613658738686 32.638700542650724 25.07148852019297 20.839197278417622 21 20.941705089299255 34.30915074414264 24.826964679652413 19.922179486905694 22 19.938623448234623 32.96843400098663 24.421631710791296 22.671310839987445 23 19.203343463098253 33.98838671925887 22.819947165765807 23.988322651877066 24 16.84288755960936 33.93115319151662 26.354544619728053 22.87141462914596 25 19.19266556613142 32.8635770527723 23.61310135246243 24.33065602863385 26 20.810794072485837 32.152429114780986 24.20038568563844 22.836391127094736 27 18.27201728965077 33.44210551043551 24.785534439421085 23.500342760492636 28 19.8198852339634 32.99854567043312 25.78178222642694 21.399786869176545 29 20.528684034622014 33.41733278947245 23.881970798087377 22.172012377818167 30 19.748556882224932 33.994366341560294 22.926512577494837 23.330564198719934 31 20.064836190382632 32.569080654426955 22.983959663176417 24.382123492014003 32 20.565202442248594 31.545497451185994 23.3158287009057 24.573471405659713 33 19.56361570675932 33.77013050525673 24.243951505263137 22.422302282720814 34 17.881206260664552 31.291790619253955 24.76887692015282 26.058126199928672 35 19.071791772466828 31.941647428655635 24.889750713817413 24.096810085060127 36 19.682353921030547 34.010596744949886 24.189067114853597 22.11798221916597 37 18.92550458402117 34.119938409890295 23.325865924054526 23.628691082034013 38 19.35539671590601 32.855034735198835 24.29776810597599 23.491800442919168 39 18.83666448125709 30.944118294013755 23.85613028742763 26.36308693730152 40 20.814638115393898 30.086042493758768 24.82119861529032 24.278120775557014 41 18.865494803067545 28.554832068714404 27.23568467743141 25.343988450786643 42 20.333278520128903 29.381941967765563 25.082166417159808 25.202613094945725 43 19.056202042895247 31.539517828884566 24.92520133174731 24.479078796472876 44 20.508396030385022 30.664998067300647 24.336849208874614 24.489756693439713 45 21.84825854178368 30.826020753560545 26.036343290116328 21.28937741453945 46 18.654072443124182 31.324464983972476 26.30393138810525 23.71753118479809 47 19.29666828258841 31.094463083306817 25.29615147237521 24.312717161729562 48 19.757526315677072 29.47889727222444 27.651695543259365 23.111880868839123 49 19.169387750743716 29.332182967900106 26.55998735736999 24.938441923986186 50 20.13808656357513 27.321962084923452 26.970659274714524 25.569292076786894 51 20.491952069056097 26.44274404867413 28.305609953508437 24.759693928761344 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 16863.0 1 12801.0 2 8739.0 3 5412.0 4 2085.0 5 1669.5 6 1254.0 7 1169.5 8 1085.0 9 1237.5 10 1390.0 11 1370.0 12 1350.0 13 1439.5 14 1529.0 15 1653.5 16 1778.0 17 1943.5 18 2109.0 19 2279.0 20 2449.0 21 2377.5 22 2306.0 23 2085.0 24 1864.0 25 2398.0 26 3450.0 27 3968.0 28 4995.5 29 6023.0 30 7084.0 31 8145.0 32 9822.5 33 11500.0 34 11191.5 35 10883.0 36 13292.5 37 15702.0 38 16541.5 39 17381.0 40 17868.0 41 18355.0 42 20540.5 43 22726.0 44 26609.5 45 30493.0 46 33305.5 47 36118.0 48 37130.0 49 38142.0 50 37580.5 51 37019.0 52 34488.5 53 31958.0 54 29283.0 55 26608.0 56 23423.0 57 20238.0 58 19332.5 59 18427.0 60 17976.0 61 17525.0 62 14305.5 63 11086.0 64 10794.0 65 10502.0 66 9489.0 67 8476.0 68 7280.5 69 6085.0 70 5444.0 71 4803.0 72 3989.5 73 3176.0 74 2892.0 75 1797.5 76 987.0 77 901.5 78 816.0 79 555.5 80 295.0 81 250.5 82 206.0 83 143.0 84 80.0 85 120.0 86 160.0 87 90.0 88 20.0 89 12.5 90 5.0 91 3.0 92 1.0 93 1.5 94 2.0 95 3.5 96 5.0 97 2.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 468257.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.72389224182679 #Duplication Level Percentage of deduplicated Percentage of total 1 78.68053669980368 20.23969647594848 2 8.830896505588358 4.543300602169594 3 3.3337367047711717 2.5727005126846905 4 1.7930299182543914 1.8449483361418983 5 1.1365752948537242 1.4618570204769856 6 0.7315878901450924 1.1291572830910663 7 0.5526312725737349 0.9951079113605257 8 0.3862522307216429 0.794872860899901 9 0.32522027691547367 0.7529338222407642 >10 2.7564283543954464 15.633529319769654 >50 0.7633057490665476 14.249387779689023 >100 0.7006026481027019 29.46591105970649 >500 0.00501624807710765 1.0193956574509258 >1k 0.003344165384738434 2.0488992465263647 >5k 0.0 0.0 >10k+ 8.360413461846085E-4 3.248302111843625 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15104 3.2255791157419966 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3436 0.7337850795610102 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2701 0.5768199941485125 No Hit CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT 2202 0.47025458241948326 Illumina PCR Primer Index 11 (95% over 24bp) TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1188 0.2537068319320373 No Hit AATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGT 960 0.20501562176326246 No Hit AACCCTGGGAACTTACATTGAAAAGCAGGCTATCCCTCCCTTCTAATCCTC 814 0.17383616262009965 No Hit AAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT 804 0.17170058322673235 Illumina PCR Primer Index 11 (95% over 21bp) AAAAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTT 792 0.16913788795469153 No Hit AATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT 752 0.16059557038122227 Illumina PCR Primer Index 11 (95% over 21bp) AATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCC 618 0.13197880651010022 No Hit GCAAGAGGGAAACACTAACTTTTTTTCTTTAGGAAGTGAATATTATTTTTT 498 0.1063518537896924 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.271158786734635E-4 0.0 0.0 0.5610167066375943 0.0 2 4.271158786734635E-4 0.0 0.0 0.736347774833051 0.0 3 4.271158786734635E-4 0.0 0.0 0.9385871433849361 0.0 4 4.271158786734635E-4 0.0 0.0 2.0384105309691045 0.0 5 4.271158786734635E-4 0.0 0.0 2.2207890111626734 0.0 6 4.271158786734635E-4 0.0 0.0 3.2166096822898536 0.0 7 4.271158786734635E-4 0.0 0.0 4.053329688611169 0.0 8 4.271158786734635E-4 0.0 0.0 4.7411998112147815 0.0 9 4.271158786734635E-4 0.0 0.0 5.72014940513436 0.0 10 4.271158786734635E-4 0.0 0.0 7.205871989100003 0.0 11 4.271158786734635E-4 0.0 0.0 8.37958642369468 0.0 12 4.271158786734635E-4 0.0 0.0 8.994206173105795 0.0 13 4.271158786734635E-4 0.0 0.0 9.247913005037832 0.0 14 4.271158786734635E-4 0.0 0.0 9.506958785453287 0.0 15 4.271158786734635E-4 0.0 0.0 9.753404647447876 0.0 16 4.271158786734635E-4 0.0 0.0 10.255052246949859 0.0 17 4.271158786734635E-4 0.0 0.0 10.85087889769934 0.0 18 4.271158786734635E-4 0.0 0.0 11.809113371503257 0.0 19 4.271158786734635E-4 0.0 0.0 12.08524378706565 0.0 20 4.271158786734635E-4 0.0 0.0 12.445516030726717 0.0 21 4.271158786734635E-4 0.0 0.0 12.784218922514773 0.0 22 4.271158786734635E-4 0.0 0.0 13.112243917335993 0.0 23 4.271158786734635E-4 0.0 0.0 13.449238345609356 0.0 24 4.271158786734635E-4 0.0 0.0 13.705934988692107 0.0 25 4.271158786734635E-4 0.0 0.0 13.934869099661084 0.0 26 4.271158786734635E-4 0.0 0.0 14.134545772940928 0.0 27 4.271158786734635E-4 0.0 0.0 14.368605274453985 0.0 28 4.271158786734635E-4 0.0 0.0 14.605227471239084 0.0 29 6.406738180101953E-4 0.0 0.0 14.821988779665867 0.0 30 6.406738180101953E-4 0.0 0.0 15.131861349643465 0.0 31 8.54231757346927E-4 0.0 0.0 15.337304087285402 0.0 32 8.54231757346927E-4 0.0 0.0 15.599766794730245 0.0 33 8.54231757346927E-4 0.0 0.0 15.837456781212026 0.0 34 8.54231757346927E-4 0.0 0.0 16.056567226971513 0.0 35 0.0010677896966836587 0.0 0.0 16.322019745567072 0.0 36 0.0010677896966836587 0.0 0.0 16.53835393811518 0.0 37 0.0012813476360203906 0.0 0.0 16.758318615632014 0.0 38 0.0012813476360203906 0.0 0.0 16.976361271694817 0.0 39 0.0014949055753571223 0.0 0.0 17.242240906169048 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAATCGA 45 3.8380676E-10 45.000004 42 TGTATCG 45 3.8380676E-10 45.000004 1 CGGGCTA 65 0.0 45.000004 6 CAAATCG 45 3.8380676E-10 45.000004 41 ACGCTCG 45 3.8380676E-10 45.000004 11 ACGTCCA 65 0.0 45.000004 27 CATCGGG 45 3.8380676E-10 45.000004 3 TAGTGCG 65 0.0 45.000004 1 TCACGGT 35 1.2098644E-7 45.0 12 TCCGGCT 25 3.887253E-5 45.0 21 TCGCCTG 25 3.887253E-5 45.0 30 TCGCCTA 20 7.0289994E-4 45.0 32 GTTGATC 35 1.2098644E-7 45.0 16 GTTGACG 35 1.2098644E-7 45.0 12 CTATGTC 25 3.887253E-5 45.0 11 CGGCGTA 20 7.0289994E-4 45.0 18 TTAGGCA 20 7.0289994E-4 45.0 44 CGTGGAC 35 1.2098644E-7 45.0 17 TTAGGAG 20 7.0289994E-4 45.0 44 AACCGCG 35 1.2098644E-7 45.0 1 >>END_MODULE