FastQCFastQC Report
Sat 18 Jun 2016
SRR3548567_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548567_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516220
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA162673.151175855255511No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38800.7516175274107939No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25390.49184456239587776No Hit
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT23480.4548448335980783TruSeq Adapter, Index 13 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17730.34345821548952No Hit
AATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCT9090.17608771454031227No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGT8790.17027623881290924No Hit
AAACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCT8090.15671612878230212No Hit
AAAAACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTT6820.1321142148696292No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCC6580.12746503428770678No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6120.11855410483902214No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGC351.2101918E-745.00000432
TTAACGG351.2101918E-745.0000042
TCAAACG700.045.0000041
ATTTACG253.8878097E-545.01
CTTCGAT406.8030204E-945.041
AACGTCA207.029671E-445.029
CTAGAAC551.8189894E-1245.037
AGCCGTA253.8878097E-545.038
GGTACGA406.8030204E-945.09
TCGCACT253.8878097E-545.032
ACCGGCA406.8030204E-945.011
GCGACGA207.029671E-445.038
GGTAATC207.029671E-445.08
CGTATGG207.029671E-445.02
CGAGTTG253.8878097E-545.030
ATAGGTG207.029671E-445.024
TTTACGG253.8878097E-545.02
GCGAATT253.8878097E-545.036
ACGTTGG253.8878097E-545.02
GACGTTG207.029671E-445.01