##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548567_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	516220
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.529458370462205	31.0	31.0	34.0	30.0	34.0
2	31.718991902677153	31.0	31.0	34.0	30.0	34.0
3	31.892524505055984	33.0	31.0	34.0	30.0	34.0
4	35.658387896633215	37.0	35.0	37.0	33.0	37.0
5	35.4233989384371	37.0	35.0	37.0	33.0	37.0
6	32.64175739025997	37.0	35.0	37.0	26.0	37.0
7	34.13091898802836	37.0	35.0	37.0	27.0	37.0
8	35.20680523807679	37.0	35.0	37.0	32.0	37.0
9	36.99537600247956	39.0	37.0	39.0	33.0	39.0
10	36.50924412072372	38.0	35.0	39.0	32.0	39.0
11	36.60739800860098	39.0	35.0	39.0	32.0	39.0
12	36.626531323854174	39.0	35.0	39.0	32.0	39.0
13	36.63009763279222	39.0	35.0	39.0	32.0	39.0
14	37.71939483166092	39.0	37.0	41.0	33.0	41.0
15	37.77151795746	39.0	37.0	41.0	33.0	41.0
16	37.69513773197474	39.0	36.0	41.0	33.0	41.0
17	37.644620510635	39.0	36.0	41.0	33.0	41.0
18	37.46467978768742	39.0	36.0	41.0	32.0	41.0
19	37.37396458874123	39.0	35.0	40.0	32.0	41.0
20	37.26869358025648	39.0	35.0	40.0	32.0	41.0
21	37.19656541784511	39.0	35.0	40.0	32.0	41.0
22	37.12473170353725	39.0	35.0	40.0	32.0	41.0
23	36.960265003293166	39.0	35.0	40.0	31.0	41.0
24	36.85883925458138	39.0	35.0	40.0	31.0	41.0
25	36.8024815001356	39.0	35.0	40.0	31.0	41.0
26	36.734283832474524	39.0	35.0	40.0	31.0	41.0
27	36.63121731044903	39.0	35.0	40.0	31.0	41.0
28	36.42638409980241	38.0	35.0	40.0	30.0	41.0
29	36.1438282127775	38.0	35.0	40.0	30.0	41.0
30	36.20651466429042	38.0	35.0	40.0	30.0	41.0
31	35.78819495563907	38.0	35.0	40.0	28.0	41.0
32	35.92379218162799	38.0	35.0	40.0	29.0	41.0
33	35.920541629537794	38.0	35.0	40.0	29.0	41.0
34	35.90717523536477	38.0	35.0	40.0	29.0	41.0
35	35.88755375615048	38.0	35.0	40.0	29.0	41.0
36	35.804135833559336	38.0	35.0	40.0	29.0	41.0
37	35.697625043586065	38.0	35.0	40.0	28.0	41.0
38	35.61051295959087	38.0	34.0	40.0	28.0	41.0
39	35.51738018674209	38.0	34.0	40.0	27.0	41.0
40	35.38935725078455	38.0	34.0	40.0	27.0	41.0
41	35.318941536554185	38.0	34.0	40.0	27.0	41.0
42	35.153186625857195	38.0	34.0	40.0	26.0	41.0
43	35.07589012436558	38.0	34.0	40.0	26.0	41.0
44	34.91836038898144	38.0	34.0	40.0	25.0	41.0
45	34.752971601255275	37.0	33.0	40.0	24.0	41.0
46	34.43996358141877	37.0	33.0	40.0	23.0	41.0
47	34.45604780907365	37.0	33.0	40.0	24.0	41.0
48	34.42606253147883	37.0	33.0	40.0	24.0	41.0
49	34.21629731509822	37.0	33.0	40.0	23.0	41.0
50	34.066136530936426	36.0	33.0	40.0	23.0	41.0
51	32.7941885242726	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	9.0
11	10.0
12	5.0
13	13.0
14	19.0
15	52.0
16	76.0
17	191.0
18	349.0
19	743.0
20	1183.0
21	1868.0
22	2542.0
23	3359.0
24	4156.0
25	4767.0
26	5389.0
27	6518.0
28	7878.0
29	9881.0
30	12358.0
31	16065.0
32	20161.0
33	26804.0
34	38089.0
35	52802.0
36	53488.0
37	69301.0
38	89842.0
39	88281.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.185231103018094	32.62717446050134	20.770795397311222	27.416799039169348
2	22.853434582154893	33.49579636589051	19.259617992328852	24.39115105962574
3	19.150168532796094	35.01375382588819	20.123784432993684	25.712293208322034
4	18.525434892100268	35.93855332997559	17.42183565146643	28.114176126457714
5	15.97361590019759	38.39448297237612	16.078416179148427	29.553484948277863
6	16.01294021928635	45.31459455271008	17.277323621711673	21.39514160629189
7	79.22668629654024	11.770756654139708	4.967455735926543	4.035101313393515
8	80.49165084653829	10.665026539072489	4.056216341869746	4.787106272519468
9	74.23811553213746	14.298748595560031	7.336213242416025	4.126922629886483
10	31.123358258107007	40.32834837859827	12.201968153113015	16.346325210181707
11	21.933477974506992	35.066638255007554	25.163496183797605	17.836387586687845
12	22.40246406570842	28.182557824183483	30.8761768238348	18.538801286273294
13	23.89388245321762	29.67416992755027	26.407926852892178	20.024020766339934
14	18.017124481810082	33.32745728565341	27.770524195110614	20.8848940374259
15	17.28739684630584	33.63895238464221	29.790593157955907	19.283057611096044
16	19.553678664135447	33.22052613226919	26.349424663902987	20.87637053969238
17	18.289101545852542	31.97473945217155	26.954786718840808	22.7813722831351
18	22.260276626244625	30.3874317151602	25.63325713842935	21.71903452016582
19	21.572391615977686	33.46228352252915	25.645073805741735	19.320251055751424
20	20.809344852969662	31.31552438882647	25.757235287280615	22.117895470923248
21	21.100693502770138	32.527023362132425	25.846150865909884	20.526132269187556
22	20.123590717136107	31.2227344930456	24.378559528883034	24.27511526093526
23	19.46573166479408	32.84045561969703	23.609701290147612	24.08411142536128
24	17.72926271744605	32.05261322691876	26.645616210142965	23.572507845492233
25	19.840378133353997	33.75130758203866	23.5105187710666	22.89779551354074
26	21.441827127968697	30.9627678121731	23.637402657781568	23.958002402076634
27	19.097477819534305	31.785479059315797	24.1209174383015	24.996125682848398
28	20.159428150788425	32.8660261128976	25.72507845492232	21.249467281391652
29	18.520979427375924	33.833055673937466	24.58506063306342	23.060904265623183
30	20.711712060749292	31.313974661965826	24.826043159893068	23.14827011739181
31	20.70396342644609	33.32145209406842	22.569640850800045	23.404943628685444
32	20.877532834837858	30.892448955871526	25.028669946921855	23.201348262368757
33	18.220138700554028	30.68943473712758	26.291309906628957	24.799116655689435
34	17.844523652706208	30.711712060749292	27.342412149858585	24.101352136685907
35	19.494014180000775	31.228158537057844	24.358413079694703	24.919414203246678
36	18.83615512765875	31.825965673550037	26.809306109798147	22.528573088993063
37	18.468482429971715	32.774011080547055	24.241214985858743	24.516291503622487
38	19.491883305567395	31.075316725427143	25.95153229243346	23.481267676572003
39	20.19313471000736	28.18333268761381	26.459455271008487	25.164077331370343
40	22.12060749292937	29.829530045329513	25.359536631668668	22.69032583007245
41	18.89640075936616	29.122273449304558	27.94138158149626	24.039944209833017
42	20.816318623842548	31.12413312153733	25.24853744527527	22.811010809344854
43	19.067645577466973	30.123978148851265	25.712293208322034	25.096083065359732
44	20.606330634225717	27.544264073457054	25.53484948277866	26.31455580953857
45	21.75874627096974	28.326488706365506	26.2304831273488	23.68428189531595
46	18.95064119948859	28.583549649374294	29.802797256983453	22.663011894153655
47	18.700554027352677	29.6193483398551	26.583045988144587	25.09705164464763
48	18.47778079113556	28.24106001317268	29.866723489984892	23.414435705706868
49	19.96474371392042	28.321064662353262	27.963077757545236	23.751113866181086
50	19.646274844058734	28.132772848785404	27.734686761458292	24.48626554569757
51	19.899655185773508	27.906125295416683	28.74162178915966	23.452597729650147
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18657.0
1	13958.0
2	9259.0
3	5684.5
4	2110.0
5	1761.5
6	1413.0
7	1406.0
8	1399.0
9	1450.0
10	1501.0
11	1815.5
12	2130.0
13	1983.5
14	1837.0
15	1846.5
16	1856.0
17	1835.5
18	1815.0
19	1798.5
20	1782.0
21	2140.0
22	2498.0
23	2622.0
24	2746.0
25	3213.5
26	3944.0
27	4207.0
28	5973.0
29	7739.0
30	9782.5
31	11826.0
32	12049.0
33	12272.0
34	11932.0
35	11592.0
36	12876.0
37	14160.0
38	15943.5
39	17727.0
40	19063.5
41	20400.0
42	23336.5
43	26273.0
44	30365.0
45	34457.0
46	36713.0
47	38969.0
48	40960.5
49	42952.0
50	41485.5
51	40019.0
52	37790.0
53	35561.0
54	31454.5
55	27348.0
56	25749.5
57	24151.0
58	22274.0
59	20397.0
60	19066.5
61	17736.0
62	16115.0
63	14494.0
64	13148.0
65	11802.0
66	10426.5
67	9051.0
68	7500.0
69	5949.0
70	5651.0
71	5353.0
72	4101.5
73	2850.0
74	2785.5
75	2007.5
76	1294.0
77	1075.5
78	857.0
79	639.0
80	421.0
81	354.5
82	288.0
83	341.0
84	394.0
85	284.0
86	174.0
87	112.0
88	50.0
89	34.0
90	18.0
91	16.0
92	14.0
93	13.0
94	12.0
95	9.5
96	7.0
97	4.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	516220.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.429578812343657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.78675778000036	21.610875818955495
2	8.834457335187473	4.846508874796247
3	3.270599065410515	2.6913346448476574
4	1.6633939943135407	1.8250478665200958
5	1.026357248251071	1.4076273515261462
6	0.6936907966353452	1.1416587826623994
7	0.5677669866086524	1.0901526514360227
8	0.41815271951721683	0.9175802380474665
9	0.31375053544878895	0.7745440535556822
>10	3.0522460306126726	18.68183408282171
>50	0.8418484145970734	16.673939868198254
>100	0.523118030123139	22.19238845414854
>500	0.004287852705927369	0.8917100958186932
>1k	0.0028585684706182455	2.0660858232422563
>5k	0.0	0.0
>10k+	7.146421176545614E-4	3.1887113934233193
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16267	3.151175855255511	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3880	0.7516175274107939	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2539	0.49184456239587776	No Hit
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT	2348	0.4548448335980783	TruSeq Adapter, Index 13 (95% over 22bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1773	0.34345821548952	No Hit
AATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCT	909	0.17608771454031227	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGT	879	0.17027623881290924	No Hit
AAACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCT	809	0.15671612878230212	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTT	682	0.1321142148696292	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCC	658	0.12746503428770678	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	612	0.11855410483902214	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.937158575801015E-4	0.0	0.0	0.5410483902212235	0.0
2	1.937158575801015E-4	0.0	0.0	0.729146487931502	0.0
3	1.937158575801015E-4	0.0	0.0	0.9588934950215025	0.0
4	1.937158575801015E-4	0.0	0.0	2.0183255201270778	0.0
5	1.937158575801015E-4	0.0	0.0	2.21359110456782	0.0
6	1.937158575801015E-4	0.0	0.0	3.2050288636627795	0.0
7	1.937158575801015E-4	0.0	0.0	3.9682693425283793	0.0
8	1.937158575801015E-4	0.0	0.0	4.572275386463136	0.0
9	1.937158575801015E-4	0.0	0.0	5.532331176630119	0.0
10	1.937158575801015E-4	0.0	0.0	6.893572507845493	0.0
11	1.937158575801015E-4	0.0	0.0	8.020611367246524	0.0
12	1.937158575801015E-4	0.0	0.0	8.676920692727906	0.0
13	1.937158575801015E-4	0.0	0.0	9.017860602068886	0.0
14	1.937158575801015E-4	0.0	0.0	9.343109526945875	0.0
15	1.937158575801015E-4	0.0	0.0	9.564526752159932	0.0
16	1.937158575801015E-4	0.0	0.0	10.098407655650691	0.0
17	1.937158575801015E-4	0.0	0.0	10.678586649103096	0.0
18	1.937158575801015E-4	0.0	0.0	11.69830692340475	0.0
19	1.937158575801015E-4	0.0	0.0	11.979969780326217	0.0
20	1.937158575801015E-4	0.0	0.0	12.40401379256906	0.0
21	1.937158575801015E-4	0.0	0.0	12.739723373755375	0.0
22	1.937158575801015E-4	0.0	0.0	13.088218201541979	0.0
23	1.937158575801015E-4	0.0	0.0	13.487854015729727	0.0
24	1.937158575801015E-4	0.0	0.0	13.729223974274534	0.0
25	1.937158575801015E-4	0.0	0.0	13.957033822788734	0.0
26	1.937158575801015E-4	0.0	0.0	14.157336019526559	0.0
27	1.937158575801015E-4	0.0	0.0	14.413234667389872	0.0
28	1.937158575801015E-4	0.0	0.0	14.649180581922437	0.0
29	1.937158575801015E-4	0.0	0.0	14.893068846615783	0.0
30	1.937158575801015E-4	0.0	0.0	15.259191817442176	0.0
31	1.937158575801015E-4	0.0	0.0	15.49630002712022	0.0
32	1.937158575801015E-4	0.0	0.0	15.747549494401612	0.0
33	1.937158575801015E-4	0.0	0.0	16.019526558444074	0.0
34	1.937158575801015E-4	0.0	0.0	16.27658750145287	0.0
35	1.937158575801015E-4	0.0	0.0	16.571229320832202	0.0
36	1.937158575801015E-4	0.0	0.0	16.81453643795281	0.0
37	1.937158575801015E-4	0.0	0.0	17.0287861764364	0.0
38	1.937158575801015E-4	0.0	0.0	17.26996241912363	0.0
39	1.937158575801015E-4	0.0	0.0	17.55298128704816	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTACGC	35	1.2101918E-7	45.000004	32
TTAACGG	35	1.2101918E-7	45.000004	2
TCAAACG	70	0.0	45.000004	1
ATTTACG	25	3.8878097E-5	45.0	1
CTTCGAT	40	6.8030204E-9	45.0	41
AACGTCA	20	7.029671E-4	45.0	29
CTAGAAC	55	1.8189894E-12	45.0	37
AGCCGTA	25	3.8878097E-5	45.0	38
GGTACGA	40	6.8030204E-9	45.0	9
TCGCACT	25	3.8878097E-5	45.0	32
ACCGGCA	40	6.8030204E-9	45.0	11
GCGACGA	20	7.029671E-4	45.0	38
GGTAATC	20	7.029671E-4	45.0	8
CGTATGG	20	7.029671E-4	45.0	2
CGAGTTG	25	3.8878097E-5	45.0	30
ATAGGTG	20	7.029671E-4	45.0	24
TTTACGG	25	3.8878097E-5	45.0	2
GCGAATT	25	3.8878097E-5	45.0	36
ACGTTGG	25	3.8878097E-5	45.0	2
GACGTTG	20	7.029671E-4	45.0	1
>>END_MODULE