FastQCFastQC Report
Sat 18 Jun 2016
SRR3548566_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548566_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398435
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA102912.582855421837941No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23080.5792663797106178No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17230.4324419290473979No Hit
CTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGCT13190.33104521440134527No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10530.26428401119379574No Hit
CAGACAGGGGAAACTAACCAACCATATAATTAACCTCCAACCCTCACACAC6470.16238533261385169No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG6190.1553578375393728No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGT5440.1365341900184472No Hit
GGACATTGGTGTTGATTATTAACAGGTAACTAAATCAGATATTTATTTGTT5430.13628320805150151No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCC5110.1282517851092399No Hit
AATCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCT4330.10867519168747725No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAT406.7957444E-945.00000424
TCACGCG302.161918E-645.0000041
TCGCCTG207.027731E-445.00000423
ACCGGAC207.027731E-445.00000445
CGAACAG207.027731E-445.0000041
ACTGCGT207.027731E-445.00000437
CAAGTTA302.161918E-645.00000419
ATTAGCA207.027731E-445.00000415
GATCGCG207.027731E-445.00000441
CCTAGTT207.027731E-445.00000428
CGAAACG207.027731E-445.0000041
CCGATCG302.161918E-645.00000426
GCGCGTT207.027731E-445.00000421
GCGCGAT207.027731E-445.00000424
TCTTCGT207.027731E-445.00000436
GACAACG406.7957444E-945.0000041
TAGCGAT302.161918E-645.00000445
ACCGATC302.161918E-645.00000425
AAGACGT207.027731E-445.00000438
GAATCGG302.161918E-645.0000042