##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548566_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	398435
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.639331384040055	31.0	31.0	34.0	30.0	34.0
2	31.85155922546965	33.0	31.0	34.0	30.0	34.0
3	31.990605744977223	33.0	31.0	34.0	30.0	34.0
4	35.72393489527777	37.0	35.0	37.0	33.0	37.0
5	35.473984464216244	37.0	35.0	37.0	33.0	37.0
6	34.07872300375218	37.0	35.0	37.0	32.0	37.0
7	34.88733168521842	37.0	35.0	37.0	32.0	37.0
8	35.58018999334898	37.0	35.0	37.0	32.0	37.0
9	37.257560204299324	39.0	37.0	39.0	34.0	39.0
10	36.71930176816796	39.0	37.0	39.0	32.0	39.0
11	36.67239072872614	39.0	35.0	39.0	32.0	39.0
12	36.55156299019915	39.0	35.0	39.0	32.0	39.0
13	36.51350157491184	39.0	35.0	39.0	32.0	39.0
14	37.656862474431215	39.0	37.0	41.0	33.0	41.0
15	37.64409251195302	39.0	36.0	41.0	33.0	41.0
16	37.58751113732478	39.0	36.0	41.0	32.0	41.0
17	37.53842659404922	39.0	36.0	41.0	32.0	41.0
18	37.41432354085359	39.0	36.0	41.0	32.0	41.0
19	37.32081518942864	39.0	35.0	41.0	32.0	41.0
20	37.23005509054175	39.0	35.0	40.0	32.0	41.0
21	37.17416893596195	39.0	35.0	40.0	32.0	41.0
22	37.17605632035338	39.0	35.0	40.0	32.0	41.0
23	36.97930653682533	39.0	35.0	40.0	31.0	41.0
24	36.79656907651185	39.0	35.0	40.0	31.0	41.0
25	36.8088169964988	39.0	35.0	40.0	31.0	41.0
26	36.70777918606548	39.0	35.0	40.0	31.0	41.0
27	36.68732164593974	39.0	35.0	40.0	31.0	41.0
28	36.56370549775998	39.0	35.0	40.0	31.0	41.0
29	36.380466073512615	38.0	35.0	40.0	30.0	41.0
30	36.38125917652817	38.0	35.0	40.0	30.0	41.0
31	36.29785535909245	38.0	35.0	40.0	30.0	41.0
32	36.21072445944759	38.0	35.0	40.0	30.0	41.0
33	36.1067752581977	38.0	35.0	40.0	30.0	41.0
34	36.00891236964624	38.0	35.0	40.0	30.0	41.0
35	35.89517487168547	38.0	35.0	40.0	29.0	41.0
36	35.79533926487382	38.0	35.0	40.0	29.0	41.0
37	35.713062356469685	38.0	35.0	40.0	29.0	41.0
38	35.587468470390405	38.0	34.0	40.0	28.0	41.0
39	35.54002283935899	38.0	34.0	40.0	28.0	41.0
40	35.37452282053535	38.0	34.0	40.0	27.0	41.0
41	35.201842207637384	38.0	34.0	40.0	26.0	41.0
42	35.05143875412551	38.0	34.0	40.0	26.0	41.0
43	34.98529998619599	37.0	34.0	40.0	26.0	41.0
44	34.85646341310377	37.0	34.0	40.0	26.0	41.0
45	34.686222344924516	37.0	33.0	40.0	25.0	41.0
46	34.54436733720682	37.0	33.0	40.0	24.0	41.0
47	34.47344736280698	37.0	33.0	40.0	24.0	41.0
48	34.32189190206684	37.0	33.0	40.0	23.0	41.0
49	34.145027419779886	36.0	33.0	40.0	23.0	41.0
50	33.993544743810155	36.0	33.0	40.0	23.0	41.0
51	32.77455293837138	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	0.0
9	0.0
10	4.0
11	5.0
12	5.0
13	5.0
14	12.0
15	28.0
16	74.0
17	138.0
18	287.0
19	544.0
20	894.0
21	1246.0
22	1759.0
23	2377.0
24	2885.0
25	3519.0
26	4079.0
27	4677.0
28	5815.0
29	7461.0
30	9520.0
31	11983.0
32	15528.0
33	20515.0
34	30036.0
35	40302.0
36	40618.0
37	53677.0
38	72123.0
39	68311.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.78180882703578	32.06646002484722	21.67128891789125	25.48044223022576
2	25.647094256277686	32.03031862160704	18.692885916146924	23.629701205968352
3	20.533336679759557	34.900548395597774	19.811261560856853	24.754853363785813
4	20.336315835707204	35.52122679985443	18.304114849348075	25.83834251509029
5	18.12390979708108	38.828165196330644	15.451955776977425	27.59596922961085
6	17.332563655301367	43.48538657497459	17.515529509204764	21.66652026051928
7	81.77419152433897	10.531454315007467	4.082472674338349	3.6118814863152084
8	83.20052204249124	9.293611254031397	3.2409301391695005	4.264936564307854
9	76.5013113807773	12.132970246087819	6.312949414584562	5.0527689585503275
10	37.43647018961688	31.93444351023379	12.500156863729343	18.128929436419995
11	26.597311983134013	33.23980072031824	22.17099401408009	17.991893282467654
12	25.457853853200646	28.326828717356655	26.94391807948599	19.271399349956706
13	25.371265074604388	28.968338624869805	26.1219521377389	19.538444162786902
14	21.36835368378782	30.491046218329217	27.229535557870165	20.9110645400128
15	19.678241118375645	32.91829282066084	27.175574434976845	20.227891625986675
16	21.540778295079498	30.6948435754891	26.578488335613088	21.185889793818312
17	21.10883832996599	30.626074516545987	27.63788321809078	20.627203935397244
18	22.28293197133786	30.589682131338865	25.861683838016237	21.26570205930704
19	20.261523209557396	34.471620214087615	25.754765520097383	19.512091056257606
20	22.86721799038739	31.631759258097308	25.571046720293143	19.929976031222157
21	22.600926123458027	31.906835493869767	26.098359832846008	19.393878549826198
22	21.791258298091282	28.879992972504926	25.43375958437386	23.89498914502993
23	20.84555824663998	31.22190570607502	25.19156198627129	22.740974061013716
24	21.453436570582404	32.09431902317819	24.808814486679132	21.64342991956028
25	21.92829445204362	31.061779211163675	24.144214238206985	22.865712098585718
26	23.00826985581086	31.96104759873003	23.673121086249953	21.35756145920916
27	19.43252977273583	29.741865046996374	27.682056044273217	23.14354913599458
28	21.703414609660296	31.739681503883947	26.1570896131113	20.39981427334446
29	20.897511513797735	33.18709450725965	24.130661211991917	21.784732766950697
30	22.35948147125629	31.405624505879253	25.0108549700704	21.22403905279406
31	23.516759320842798	32.24013954597362	22.11929172888928	22.1238094042943
32	22.682495262715374	30.95210009160842	23.66584260920853	22.69956203646768
33	21.90445116518378	30.889103617905057	24.7641396965628	22.442305520348363
34	21.038061415287313	32.32396752293348	24.249877646291115	22.388093415488097
35	22.421975981025764	30.31109214802916	26.272290335939363	20.99464153500571
36	24.42832582478949	30.91821752607075	25.242009361627364	19.41144728751239
37	23.261008696525153	32.56742003087078	23.214075068706315	20.957496203897747
38	23.270044047335197	31.128540414371226	24.571134563981577	21.030280974311996
39	22.9392498149008	29.95394480906547	24.452169111649326	22.654636264384404
40	24.609534804924266	29.10562576078909	24.954133045540676	21.330706388745966
41	23.10866264258913	29.109892454227165	26.557656832356596	21.22378807082711
42	21.68534390804021	30.57236437561961	25.345915895942877	22.396375820397303
43	21.7089362129331	28.724635134965553	27.34749708233463	22.218931569766713
44	21.811336855446935	26.724810822342416	26.87012938120396	24.59372294100669
45	22.56428275628396	30.247342728424965	26.47884849473565	20.709526020555423
46	20.70174557958011	29.777002522368768	27.56409451980875	21.95715737824237
47	21.27549035601792	29.898979758304367	26.986835995833697	21.838693889844016
48	22.207135417320263	29.101861031284905	27.52293347722966	21.168070074165172
49	22.615734059507826	28.334609158331975	27.62859688531379	21.421059896846412
50	22.508564759622022	27.689585503281588	27.338712713491535	22.463137023604855
51	20.219860203044412	28.652101346518254	27.998544304591714	23.129494145845623
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11778.0
1	8844.5
2	5911.0
3	3655.0
4	1399.0
5	1152.5
6	906.0
7	913.5
8	921.0
9	992.5
10	1064.0
11	1165.0
12	1266.0
13	1298.0
14	1330.0
15	1380.0
16	1430.0
17	1429.0
18	1428.0
19	1461.0
20	1494.0
21	1498.5
22	1503.0
23	1726.0
24	1949.0
25	2105.5
26	3426.0
27	4590.0
28	5928.5
29	7267.0
30	7330.5
31	7394.0
32	8159.5
33	8925.0
34	10271.0
35	11617.0
36	12455.5
37	13294.0
38	13516.5
39	13739.0
40	15126.0
41	16513.0
42	16948.0
43	17383.0
44	20726.0
45	24069.0
46	26448.0
47	28827.0
48	29399.5
49	29972.0
50	29877.0
51	29782.0
52	28169.5
53	26557.0
54	23318.0
55	20079.0
56	18386.5
57	16694.0
58	16686.5
59	16679.0
60	16379.5
61	16080.0
62	14439.5
63	12799.0
64	12107.5
65	11416.0
66	10381.0
67	9346.0
68	7969.0
69	6592.0
70	5940.5
71	5289.0
72	4307.5
73	3326.0
74	2762.0
75	1704.5
76	1211.0
77	988.0
78	765.0
79	732.5
80	700.0
81	515.5
82	331.0
83	286.0
84	241.0
85	161.5
86	82.0
87	50.5
88	19.0
89	15.0
90	11.0
91	6.5
92	2.0
93	3.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	398435.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.31711412455125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.78245607990584	19.692273174628713
2	9.014851252121352	4.5646003593162
3	3.3647707083471188	2.5555885207851343
4	1.7578094568784797	1.7801065051603167
5	1.032886595587426	1.307485390910304
6	0.6679266335648919	1.0145984885273815
7	0.5039446448385211	0.8930896860083298
8	0.3699593622607401	0.7493042716641085
9	0.2779694667791311	0.6333646242229127
>10	3.5026152594504536	21.1273427408635
>50	1.160872485361683	20.712187671428847
>100	0.5539391532216816	20.019081220140993
>500	0.004999450841350917	0.725002511500516
>1k	0.003999560673080734	1.6208767741402945
>5k	0.0	0.0
>10k+	9.998901682701834E-4	2.6050980607024474
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10291	2.582855421837941	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2308	0.5792663797106178	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1723	0.4324419290473979	No Hit
CTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGCT	1319	0.33104521440134527	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1053	0.26428401119379574	No Hit
CAGACAGGGGAAACTAACCAACCATATAATTAACCTCCAACCCTCACACAC	647	0.16238533261385169	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	619	0.1553578375393728	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGT	544	0.1365341900184472	No Hit
GGACATTGGTGTTGATTATTAACAGGTAACTAAATCAGATATTTATTTGTT	543	0.13628320805150151	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCC	511	0.1282517851092399	No Hit
AATCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCT	433	0.10867519168747725	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.39730445367500344	0.0
2	0.0	0.0	0.0	0.5172738338750361	0.0
3	0.0	0.0	0.0	0.6633453386374188	0.0
4	0.0	0.0	0.0	1.3477731624982745	0.0
5	0.0	0.0	0.0	1.4531855886154579	0.0
6	0.0	0.0	0.0	2.145644835418575	0.0
7	0.0	0.0	0.0	2.6656794709300136	0.0
8	0.0	0.0	0.0	3.2268751490205427	0.0
9	0.0	0.0	0.0	4.008182012122429	0.0
10	0.0	0.0	0.0	5.142871484683825	0.0
11	0.0	0.0	0.0	6.059959591903322	0.0
12	0.0	0.0	0.0	6.507711420934406	0.0
13	0.0	0.0	0.0	6.70071655351563	0.0
14	0.0	0.0	0.0	6.903008018873844	0.0
15	0.0	0.0	0.0	7.091746458016991	0.0
16	0.0	0.0	0.0	7.467215480567721	0.0
17	0.0	0.0	0.0	7.92224578664023	0.0
18	0.0	0.0	0.0	8.584587197409865	0.0
19	0.0	0.0	0.0	8.813984715198213	0.0
20	0.0	0.0	0.0	9.069735339515855	0.0
21	0.0	0.0	0.0	9.342050773651913	0.0
22	0.0	0.0	0.0	9.633189855308895	0.0
23	0.0	0.0	0.0	9.97703515002447	0.0
24	0.0	0.0	0.0	10.200660082573068	0.0
25	0.0	0.0	0.0	10.404708421699901	0.0
26	0.0	0.0	0.0	10.571360447751829	0.0
27	0.0	0.0	0.0	10.780930390151468	0.0
28	0.0	0.0	0.0	10.978202216170768	0.0
29	0.0	0.0	0.0	11.186266266768733	0.0
30	0.0	0.0	0.0	11.426957973069635	0.0
31	0.0	0.0	0.0	11.637029879403165	0.0
32	0.0	0.0	0.0	11.846348839835859	0.0
33	0.0	0.0	0.0	12.084530726467303	0.0
34	0.0	0.0	0.0	12.281049606585766	0.0
35	0.0	0.0	0.0	12.502415701431852	0.0
36	0.0	0.0	0.0	12.708220914327306	0.0
37	0.0	0.0	0.0	12.91277121738803	0.0
38	0.0	0.0	0.0	13.141415789275541	0.0
39	0.0	0.0	0.0	13.363785811989409	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAT	40	6.7957444E-9	45.000004	24
TCACGCG	30	2.161918E-6	45.000004	1
TCGCCTG	20	7.027731E-4	45.000004	23
ACCGGAC	20	7.027731E-4	45.000004	45
CGAACAG	20	7.027731E-4	45.000004	1
ACTGCGT	20	7.027731E-4	45.000004	37
CAAGTTA	30	2.161918E-6	45.000004	19
ATTAGCA	20	7.027731E-4	45.000004	15
GATCGCG	20	7.027731E-4	45.000004	41
CCTAGTT	20	7.027731E-4	45.000004	28
CGAAACG	20	7.027731E-4	45.000004	1
CCGATCG	30	2.161918E-6	45.000004	26
GCGCGTT	20	7.027731E-4	45.000004	21
GCGCGAT	20	7.027731E-4	45.000004	24
TCTTCGT	20	7.027731E-4	45.000004	36
GACAACG	40	6.7957444E-9	45.000004	1
TAGCGAT	30	2.161918E-6	45.000004	45
ACCGATC	30	2.161918E-6	45.000004	25
AAGACGT	20	7.027731E-4	45.000004	38
GAATCGG	30	2.161918E-6	45.000004	2
>>END_MODULE