##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548563_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	660954
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.486425681666198	31.0	31.0	34.0	30.0	34.0
2	31.706802591405754	31.0	31.0	34.0	30.0	34.0
3	31.894451051056503	33.0	31.0	34.0	30.0	34.0
4	35.62053183731394	37.0	35.0	37.0	33.0	37.0
5	35.37060975499051	37.0	35.0	37.0	33.0	37.0
6	32.64333372670413	37.0	35.0	37.0	26.0	37.0
7	34.14193574741964	37.0	35.0	37.0	27.0	37.0
8	35.207758785028915	37.0	35.0	37.0	32.0	37.0
9	37.025888337161135	39.0	37.0	39.0	34.0	39.0
10	36.54123131110486	38.0	35.0	39.0	32.0	39.0
11	36.62272563597467	39.0	35.0	39.0	32.0	39.0
12	36.57213512589379	39.0	35.0	39.0	32.0	39.0
13	36.57049809820351	39.0	35.0	39.0	32.0	39.0
14	37.66358173186031	39.0	37.0	41.0	33.0	41.0
15	37.70369193620131	39.0	37.0	41.0	33.0	41.0
16	37.626471736308424	39.0	36.0	41.0	32.0	41.0
17	37.62255013208181	39.0	36.0	41.0	32.0	41.0
18	37.47497556562181	39.0	36.0	41.0	32.0	41.0
19	37.42537150845596	39.0	36.0	41.0	32.0	41.0
20	37.31386299197826	39.0	35.0	40.0	32.0	41.0
21	37.260307071293916	39.0	35.0	40.0	32.0	41.0
22	37.181828084859156	39.0	35.0	40.0	32.0	41.0
23	37.02082595763094	39.0	35.0	40.0	31.0	41.0
24	36.891546159036785	39.0	35.0	40.0	31.0	41.0
25	36.87604432381073	39.0	35.0	40.0	31.0	41.0
26	36.794843514072085	39.0	35.0	40.0	31.0	41.0
27	36.717901699664424	39.0	35.0	40.0	31.0	41.0
28	36.59695531005183	39.0	35.0	40.0	30.0	41.0
29	36.38336858540836	38.0	35.0	40.0	30.0	41.0
30	36.42604023880633	39.0	35.0	40.0	30.0	41.0
31	36.01289197130209	38.0	35.0	40.0	29.0	41.0
32	36.181024700659954	38.0	35.0	40.0	30.0	41.0
33	36.17162011274612	38.0	35.0	40.0	30.0	41.0
34	36.11186708908638	38.0	35.0	40.0	30.0	41.0
35	36.134950389890975	38.0	35.0	40.0	30.0	41.0
36	36.04237662530222	38.0	35.0	40.0	30.0	41.0
37	35.93441449783192	38.0	35.0	40.0	29.0	41.0
38	35.81230009955307	38.0	35.0	40.0	29.0	41.0
39	35.72685845005855	38.0	35.0	40.0	29.0	41.0
40	35.57398699455636	38.0	34.0	40.0	28.0	41.0
41	35.53946265549494	38.0	34.0	40.0	28.0	41.0
42	35.42745486070135	38.0	34.0	40.0	27.0	41.0
43	35.35497629184482	38.0	34.0	40.0	27.0	41.0
44	35.1597282110404	38.0	34.0	40.0	26.0	41.0
45	34.98469485017112	37.0	34.0	40.0	26.0	41.0
46	34.70358905460894	37.0	33.0	40.0	24.0	41.0
47	34.65754500313184	37.0	33.0	40.0	24.0	41.0
48	34.65036598613519	37.0	33.0	40.0	25.0	41.0
49	34.49625843856002	37.0	33.0	40.0	24.0	41.0
50	34.32840560765197	37.0	33.0	40.0	24.0	41.0
51	33.186792121690765	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	5.0
10	9.0
11	13.0
12	17.0
13	22.0
14	30.0
15	58.0
16	121.0
17	218.0
18	441.0
19	879.0
20	1342.0
21	1973.0
22	2733.0
23	3589.0
24	4386.0
25	5195.0
26	6398.0
27	7803.0
28	9690.0
29	12337.0
30	15837.0
31	20297.0
32	25615.0
33	34304.0
34	48257.0
35	65522.0
36	70937.0
37	91628.0
38	118662.0
39	112615.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.13673266218224	32.791540712364245	20.29142724001973	27.780299385433782
2	22.667992023650662	33.06145359586295	19.591227226100454	24.679327154385934
3	21.41934234455045	33.52547983672086	19.319498785089433	25.735679033639254
4	20.763018303845655	33.875731140139855	18.85184142920687	26.509409126807615
5	17.357335003646245	37.28882796684792	16.35923226124662	28.994604768259215
6	16.789216798748477	45.43145211315765	16.609779197947212	21.169551890146668
7	79.78633914009144	10.283469046257379	5.372839864801484	4.557351948849693
8	80.97477282836627	9.301706321468666	4.707438036535069	5.016082813629995
9	75.18374954989304	12.32400439364918	7.646825648986162	4.845420407471624
10	31.9309664515231	36.56941330258989	13.66676047047147	17.832859775415535
11	24.054472777228067	32.29543962212196	24.45344154056107	19.196646060088902
12	23.78743452645721	27.912078601536567	28.977508268351503	19.322978603654718
13	24.572511854077593	27.960796061450573	26.49745670651815	20.969235377953684
14	19.381832926345858	30.785954847084668	27.395703785740004	22.436508440829467
15	18.533967568090972	32.66823409798564	28.45916659858326	20.33863173534013
16	19.176069741615905	31.678755253769552	27.369983387648762	21.77519161696578
17	20.376758443098915	29.48541048242389	27.126244791619385	23.01158628285781
18	20.428653128659484	29.48314103553349	26.356750999313117	23.731454836493917
19	19.57095350054618	32.37441637390802	26.56478363093347	21.489846494612333
20	21.584255485253134	31.210341415590193	25.414627946876788	21.79077515227989
21	21.8124105459684	30.094378731348932	26.245396805223965	21.8478139174587
22	19.760376667665223	30.234176659797807	25.529764552449947	24.475682120087026
23	19.942991494113055	31.339699888343215	24.83546510044572	23.881843517098016
24	18.90176926079576	31.555448639390942	26.34222653921453	23.200555560598772
25	20.527601013081092	30.513016034398767	25.36984419490615	23.589538757613994
26	21.17272911579323	31.169945260941002	24.077015949672745	23.58030967359302
27	19.012215676128747	30.881876802319074	25.190860483482965	24.915047038069215
28	20.02907917948904	30.150812310690306	26.575071790169968	23.245036719650685
29	21.097837368409905	30.460213570082033	25.267416491919253	23.174532569588806
30	20.936404046272507	29.744732613767372	24.593693358388027	24.72516998157209
31	20.505511730014494	31.758639784311764	23.021269256256865	24.714579229416874
32	19.993827104458102	28.904129485561782	26.61047516166027	24.491568248319854
33	20.296722616097338	29.679675136242462	24.802482472305183	25.221119775355017
34	18.461042674679327	29.5836018845487	26.882808788508733	25.072546652263245
35	19.467618018803122	28.19454909116217	27.51371502404101	24.8241178659937
36	21.020978767054892	29.72158425548525	27.174205769236586	22.083231208223264
37	19.943294087031777	29.875301458195274	26.54087879035455	23.6405256644184
38	20.024691582167595	29.215951488303272	28.620146031342575	22.13921089818656
39	21.859917634207523	27.225192676040994	27.24425602992039	23.670633659831093
40	21.8447879882715	27.842784823149568	27.297663680074557	23.014763508504373
41	19.132193768401432	27.154234636601032	30.281381155118208	23.432190439879324
42	21.02914877586035	28.287142524290648	26.519999878962828	24.163708820886175
43	18.70977405386759	28.181688892116547	28.244779515669777	24.863757538346086
44	21.268953663946355	26.569776414092356	27.865630588512968	24.295639333448317
45	21.277577562129892	27.400091383061454	27.47997591360367	23.842355141204983
46	19.32464286470768	27.82024165070489	29.802376564783632	23.052738919803797
47	20.32138393897306	27.810709973765196	27.771675487250246	24.0962306000115
48	20.20291881129398	26.390641406209813	29.91705928097871	23.489380501517505
49	20.752881441068517	27.731430629060416	27.99514035772535	23.520547572145716
50	19.75235795531913	26.209690840814943	28.79912974276576	25.23882146110017
51	20.568299760649	25.816773935856354	29.517939221186346	24.0969870823083
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18149.0
1	13704.5
2	9260.0
3	5784.0
4	2308.0
5	1868.0
6	1428.0
7	1427.5
8	1427.0
9	1445.5
10	1464.0
11	1565.5
12	1667.0
13	1757.0
14	1847.0
15	1955.0
16	2063.0
17	2235.5
18	2408.0
19	2299.5
20	2191.0
21	2561.0
22	2931.0
23	3799.5
24	4668.0
25	5219.5
26	5340.5
27	4910.0
28	6311.0
29	7712.0
30	9497.0
31	11282.0
32	12855.5
33	14429.0
34	16853.0
35	19277.0
36	20473.5
37	21670.0
38	24165.0
39	26660.0
40	26331.0
41	26002.0
42	29395.0
43	32788.0
44	36698.0
45	40608.0
46	45231.5
47	49855.0
48	52574.0
49	55293.0
50	53844.5
51	52396.0
52	49238.0
53	46080.0
54	41810.0
55	37540.0
56	33791.0
57	30042.0
58	28929.0
59	27816.0
60	25977.5
61	24139.0
62	20880.0
63	17621.0
64	16965.5
65	16310.0
66	14979.5
67	13649.0
68	10918.5
69	8188.0
70	7120.0
71	6052.0
72	5257.5
73	4463.0
74	3871.5
75	2699.0
76	2118.0
77	1665.5
78	1213.0
79	1092.0
80	971.0
81	719.0
82	467.0
83	330.0
84	193.0
85	212.0
86	231.0
87	145.0
88	59.0
89	43.0
90	27.0
91	24.5
92	22.0
93	14.5
94	7.0
95	4.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	660954.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.610528253666743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.49369746792975	19.846545284835173
2	9.403633694890809	4.816640528602672
3	3.540172533753158	2.7199706609562067
4	1.8109216536429256	1.8551464070319614
5	1.1328732019162664	1.4506740572749222
6	0.7951886864025537	1.2219121392065246
7	0.5716695397377641	1.0248531229450268
8	0.4116418336308666	0.8433891848475594
9	0.3284416313679239	0.757040731184575
>10	2.971112946691828	17.089878446219828
>50	0.8763586158678194	16.272746600744888
>100	0.6559248535943188	26.91261349382616
>500	0.004181670287031177	0.6870895422651231
>1k	0.003584288817455294	2.0961509282062907
>5k	0.0	0.0
>10k+	5.973814695758823E-4	2.4053488718530986
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15722	2.378682934061977	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3762	0.5691772801132908	No Hit
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT	3202	0.4844512628715463	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2775	0.4198476747247161	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1842	0.27868807814159535	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTT	1071	0.16203850797483638	No Hit
AAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT	1049	0.1587099858689107	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGT	869	0.13147662318406422	No Hit
AATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT	767	0.11604438432931792	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5129645936025804E-4	0.0	0.0	0.586273780021	0.0
2	1.5129645936025804E-4	0.0	0.0	0.7688886064688314	0.0
3	1.5129645936025804E-4	0.0	0.0	0.97919068497959	0.0
4	1.5129645936025804E-4	0.0	0.0	2.041140533229241	0.0
5	1.5129645936025804E-4	0.0	0.0	2.2217885057053897	0.0
6	1.5129645936025804E-4	0.0	0.0	3.2776864955806304	0.0
7	1.5129645936025804E-4	0.0	0.0	4.1741180172901595	0.0
8	1.5129645936025804E-4	0.0	0.0	4.8107735182781255	0.0
9	1.5129645936025804E-4	0.0	0.0	5.737010442481625	0.0
10	1.5129645936025804E-4	0.0	0.0	7.089298196243612	0.0
11	1.5129645936025804E-4	0.0	0.0	8.15563564181471	0.0
12	1.5129645936025804E-4	0.0	0.0	8.786541877346986	0.0
13	1.5129645936025804E-4	0.0	0.0	9.071584406781712	0.0
14	1.5129645936025804E-4	0.0	0.0	9.33922784339001	0.0
15	1.5129645936025804E-4	0.0	0.0	9.585387182769148	0.0
16	1.5129645936025804E-4	0.0	0.0	10.071502706693657	0.0
17	1.5129645936025804E-4	0.0	0.0	10.659894637145701	0.0
18	1.5129645936025804E-4	0.0	0.0	11.605043618769233	0.0
19	1.5129645936025804E-4	0.0	0.0	11.886757626098033	0.0
20	1.5129645936025804E-4	0.0	0.0	12.259854694880431	0.0
21	1.5129645936025804E-4	0.0	0.0	12.59951524614421	0.0
22	1.5129645936025804E-4	0.0	0.0	12.979571952057178	0.0
23	1.5129645936025804E-4	0.0	0.0	13.351004759786612	0.0
24	1.5129645936025804E-4	0.0	0.0	13.632718767115412	0.0
25	1.5129645936025804E-4	0.0	0.0	13.884778668409602	0.0
26	1.5129645936025804E-4	0.0	0.0	14.111118171612548	0.0
27	1.5129645936025804E-4	0.0	0.0	14.379820683436366	0.0
28	1.5129645936025804E-4	0.0	0.0	14.644892080235538	0.0
29	1.5129645936025804E-4	0.0	0.0	14.9039116186603	0.0
30	1.5129645936025804E-4	0.0	0.0	15.245387727436402	0.0
31	1.5129645936025804E-4	0.0	0.0	15.493816513705946	0.0
32	1.5129645936025804E-4	0.0	0.0	15.754500313183671	0.0
33	1.5129645936025804E-4	0.0	0.0	16.019874302901563	0.0
34	1.5129645936025804E-4	0.0	0.0	16.272539390033195	0.0
35	1.5129645936025804E-4	0.0	0.0	16.534130968267082	0.0
36	3.025929187205161E-4	0.0	0.0	16.773179374056287	0.0
37	4.5388937808077417E-4	0.0	0.0	17.011017408170613	0.0
38	4.5388937808077417E-4	0.0	0.0	17.27291157932322	0.0
39	4.5388937808077417E-4	0.0	0.0	17.548271135358892	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGGGC	130	0.0	45.000004	4
TAGCGAT	55	1.8189894E-12	45.000004	45
CCGACAG	55	1.8189894E-12	45.000004	1
CTTGTCA	20	7.031108E-4	45.0	33
AAACTCG	20	7.031108E-4	45.0	20
AATCCGA	40	6.8066583E-9	45.0	36
TTGGCGC	35	1.210883E-7	45.0	30
ATCTCGC	20	7.031108E-4	45.0	41
GTCGCAA	25	3.889001E-5	45.0	33
TCACCGC	20	7.031108E-4	45.0	35
CGTATCT	25	3.889001E-5	45.0	16
AGTTTAC	20	7.031108E-4	45.0	17
GGTAAGC	40	6.8066583E-9	45.0	8
TTGTGCG	25	3.889001E-5	45.0	11
AGTCACG	20	7.031108E-4	45.0	38
TTAGCTA	25	3.889001E-5	45.0	43
CGAAACT	20	7.031108E-4	45.0	43
CGAAACG	25	3.889001E-5	45.0	1
ACGCAAT	25	3.889001E-5	45.0	12
TTGCTCG	40	6.8066583E-9	45.0	1
>>END_MODULE