##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548560_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 695276 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.686892687220613 31.0 31.0 34.0 30.0 34.0 2 31.890181740776324 33.0 31.0 34.0 30.0 34.0 3 32.06215517290975 33.0 31.0 34.0 30.0 34.0 4 35.784645234410505 37.0 35.0 37.0 35.0 37.0 5 35.53425977597386 37.0 35.0 37.0 33.0 37.0 6 34.144463493634184 37.0 35.0 37.0 32.0 37.0 7 34.97851644526778 37.0 35.0 37.0 32.0 37.0 8 35.70659277754446 37.0 35.0 37.0 33.0 37.0 9 37.403034190738644 39.0 37.0 39.0 35.0 39.0 10 36.74923052140445 39.0 37.0 39.0 32.0 39.0 11 36.7718848342241 39.0 37.0 39.0 32.0 39.0 12 36.82365708006605 39.0 37.0 39.0 33.0 39.0 13 36.81161581875399 39.0 37.0 39.0 33.0 39.0 14 37.95728315086383 40.0 37.0 41.0 33.0 41.0 15 37.97740321829029 40.0 37.0 41.0 33.0 41.0 16 37.894794297516384 40.0 37.0 41.0 33.0 41.0 17 37.86912391625771 39.0 37.0 41.0 33.0 41.0 18 37.734603236700245 39.0 37.0 41.0 33.0 41.0 19 37.699169826083455 39.0 36.0 41.0 33.0 41.0 20 37.59533192573884 39.0 36.0 41.0 32.0 41.0 21 37.54849728740817 39.0 36.0 41.0 32.0 41.0 22 37.565433007899024 39.0 36.0 41.0 33.0 41.0 23 37.37742565542317 39.0 36.0 41.0 32.0 41.0 24 37.210246003026135 39.0 35.0 41.0 32.0 41.0 25 37.28676525581208 39.0 35.0 41.0 32.0 41.0 26 37.19687721135204 39.0 35.0 41.0 32.0 41.0 27 37.13586690753025 39.0 35.0 41.0 32.0 41.0 28 37.07232523487075 39.0 35.0 41.0 31.0 41.0 29 36.91568240526065 39.0 35.0 40.0 31.0 41.0 30 36.93010401624679 39.0 35.0 40.0 31.0 41.0 31 36.8493418441022 39.0 35.0 40.0 31.0 41.0 32 36.7407044109102 39.0 35.0 40.0 31.0 41.0 33 36.68267278030595 39.0 35.0 40.0 31.0 41.0 34 36.54973995938304 39.0 35.0 40.0 30.0 41.0 35 36.45496177057744 39.0 35.0 40.0 30.0 41.0 36 36.40713903543341 39.0 35.0 40.0 30.0 41.0 37 36.409722182270066 39.0 35.0 40.0 30.0 41.0 38 36.2906500440113 39.0 35.0 40.0 30.0 41.0 39 36.243028667752085 39.0 35.0 40.0 30.0 41.0 40 36.075844700521806 38.0 35.0 40.0 30.0 41.0 41 35.98690160454266 38.0 35.0 40.0 29.0 41.0 42 35.91244196549284 38.0 35.0 40.0 29.0 41.0 43 35.817748635074416 38.0 35.0 40.0 29.0 41.0 44 35.69379642041434 38.0 35.0 40.0 28.0 41.0 45 35.55359598202728 38.0 34.0 40.0 28.0 41.0 46 35.42264079300882 38.0 34.0 40.0 27.0 41.0 47 35.33676554346763 38.0 34.0 40.0 27.0 41.0 48 35.221289099580595 38.0 34.0 40.0 26.0 41.0 49 35.041843814542716 38.0 34.0 40.0 26.0 41.0 50 34.92938631565019 37.0 34.0 40.0 26.0 41.0 51 33.84875646505848 36.0 32.0 39.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 5.0 10 8.0 11 6.0 12 11.0 13 12.0 14 25.0 15 32.0 16 95.0 17 190.0 18 367.0 19 696.0 20 1162.0 21 1704.0 22 2579.0 23 3356.0 24 4211.0 25 5043.0 26 5718.0 27 6944.0 28 8445.0 29 10651.0 30 13658.0 31 17442.0 32 22965.0 33 30803.0 34 44245.0 35 61738.0 36 69035.0 37 94033.0 38 138773.0 39 151307.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.24997842583377 32.86550952427525 19.910078875151736 25.974433174739243 2 22.682646891306472 32.666020400531586 19.396182235543872 25.25515047261807 3 20.326316455623378 34.93288996024601 19.934241941329773 24.806551642800844 4 19.564892215465512 36.00699578296964 18.35199258999304 26.076119411571806 5 17.104430470777075 38.067903969071274 16.43620662873449 28.391458931417162 6 17.726341769311755 43.328980146013954 16.42455657897008 22.52012150570421 7 84.51478261870106 9.66134312129284 3.1101318037728904 2.713742456233208 8 86.82940299967207 8.194587473176119 2.44694193385073 2.5290675933010776 9 79.66361560013577 12.696540654358845 4.570702857570231 3.0691408879351507 10 29.14871216610382 46.67081849510122 11.650337419959843 12.53013191883511 11 18.870060235072113 31.91883511008578 32.27092550296573 16.94017915187638 12 23.57811861764249 26.984104154321447 32.34931164026948 17.088465587766585 13 23.2865797179825 27.33561923610192 30.56527192079117 18.81252912512441 14 18.552776163710526 32.75389917097671 28.797484739873084 19.89583992543968 15 18.10245140059487 34.4668879696696 29.290814007674648 18.13984662206088 16 19.922735719340235 32.42395825542662 28.161765974950953 19.49154005028219 17 19.81026239939247 31.90128812155173 28.38786323704543 19.90058624201037 18 19.486793733711504 32.2008813766044 28.562613983511586 19.749710906172513 19 19.9654525684764 34.68737594854417 27.71532456175677 17.631846921222653 20 21.0556671019854 32.96561365558426 27.768828494008137 18.209890748422207 21 21.459247838268546 31.673896409483426 28.89974628780513 17.9671094644429 22 21.11736921740431 29.689504599612242 27.472399450002587 21.72072673298086 23 18.959521111040793 34.169883614564576 27.20746868869341 19.663126585701217 24 17.403448414730267 34.2671111903762 28.84768063330246 19.481759761591082 25 20.235129646356267 33.18940967328083 25.898923592932878 20.676537087430027 26 20.83776802305847 33.74746143977356 25.082413315000085 20.33235722216789 27 17.943665537139207 32.748289887756805 27.763363038563106 21.544681536540885 28 19.286153987768888 33.00617308809739 27.9996720726733 19.708000851460426 29 19.928201174785265 32.51629568689269 26.80503857460922 20.75046456371283 30 20.09072656038753 32.28962311369873 27.545032476311565 20.074617849602173 31 20.31250898923593 33.50194742807173 25.473049551544996 20.712494031147344 32 20.551406923293772 32.32414177966735 27.199558161075604 19.924893135963273 33 20.344438755256906 32.924335084196784 25.679730064032125 21.051496096514192 34 18.047365362819946 33.51446044448536 28.062380982516295 20.375793210178404 35 19.39014146899936 31.825778539745368 28.03390308309218 20.75017690816309 36 19.470828850701018 34.122420448857724 26.27345111869243 20.13329958174883 37 20.407435320649643 32.859037274406134 26.139115976964543 20.59441142797968 38 19.521743883004735 32.55225263061 27.926607563039713 19.99939592334555 39 19.71734965682693 30.438128167806745 27.489658782986897 22.35486339237943 40 21.04171580782308 31.653329037677125 26.346659456100884 20.95829569839891 41 19.127655779862963 30.049217864560262 29.142527571784445 21.680598783792334 42 20.439940397770094 31.967305070216717 25.444140168796274 22.148614363216907 43 18.757155431799745 32.63509742893469 26.93793543858842 21.66981170067714 44 20.731047814105477 31.860872516813465 26.945702138431354 20.462377530649697 45 21.840391441672082 30.013260920842942 27.611624736076035 20.534722901408937 46 19.332754186826527 31.383795787572126 27.961270056783206 21.32217996881814 47 19.336781364522864 32.577566318986996 27.805792232149535 20.279860084340605 48 19.546482260282247 30.696730507021673 28.734632002255218 21.022155230440863 49 20.502217824288486 31.205737002283986 27.262410898693467 21.029634274734065 50 18.36723833412918 30.32594250340872 28.823086083799815 22.483733078662286 51 18.461589354443415 30.77454133322594 28.14119860314465 22.622670709185993 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 17277.0 1 13419.0 2 9561.0 3 6179.5 4 2798.0 5 2259.0 6 1720.0 7 1931.5 8 2143.0 9 2486.0 10 2829.0 11 3095.5 12 3362.0 13 3545.0 14 3728.0 15 3875.0 16 4022.0 17 3633.5 18 3245.0 19 3720.0 20 4195.0 21 4245.0 22 4295.0 23 5036.5 24 5778.0 25 7162.0 26 9825.5 27 11105.0 28 12745.0 29 14385.0 30 15576.5 31 16768.0 32 19764.0 33 22760.0 34 25434.5 35 28109.0 36 30856.5 37 33604.0 38 32543.5 39 31483.0 40 32285.5 41 33088.0 42 35865.5 43 38643.0 44 44100.5 45 49558.0 46 48392.0 47 47226.0 48 50119.5 49 53013.0 50 51670.0 51 50327.0 52 46777.0 53 43227.0 54 37852.5 55 32478.0 56 29598.5 57 26719.0 58 24310.0 59 21901.0 60 20397.0 61 18893.0 62 16702.0 63 14511.0 64 12510.0 65 10509.0 66 9404.5 67 8300.0 68 6563.0 69 4826.0 70 3853.5 71 2881.0 72 2919.5 73 2958.0 74 2374.5 75 1567.5 76 1344.0 77 997.5 78 651.0 79 521.0 80 391.0 81 241.0 82 91.0 83 106.5 84 122.0 85 100.0 86 78.0 87 45.0 88 12.0 89 10.0 90 8.0 91 10.5 92 13.0 93 8.5 94 4.0 95 2.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 695276.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.531276772767615 #Duplication Level Percentage of deduplicated Percentage of total 1 76.650570673375 18.803363639791467 2 8.982773651166845 4.407178132477963 3 3.3932791703704184 2.49724411486872 4 1.8270682734637433 1.7928126999632703 5 1.20799871022989 1.4816875350897867 6 0.838938893769634 1.234814531910143 7 0.6410432871399441 1.1007927210108297 8 0.47335179222649265 0.9289539060794945 9 0.38819720440209776 0.8570675757242253 >10 4.039073647696875 21.948377093490056 >50 0.9385333734180052 16.346193530437382 >100 0.6126537298700868 24.384332926496885 >500 0.0029625422140719866 0.46221263750803276 >1k 0.0029625422140719866 1.5758834641075758 >5k 0.0 0.0 >10k+ 5.925084428143973E-4 2.1790854910441597 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14992 2.1562660008399543 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3528 0.5074243897387513 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3004 0.4320586357072587 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1620 0.23300099528820206 No Hit CTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGCT 1484 0.21344041790598267 No Hit GGCCTCAGGGATTTTTTTTTCTTTTAGAATGTAAGCTCGTCTAGAGCAGGG 1206 0.17345629649232822 No Hit ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA 846 0.12167829753939442 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.258889994764669 0.0 2 0.0 0.0 0.0 0.3589941260736743 0.0 3 0.0 0.0 0.0 0.456509357435033 0.0 4 0.0 0.0 0.0 0.9541534584826745 0.0 5 0.0 0.0 0.0 1.0497989287707328 0.0 6 0.0 0.0 0.0 1.6095766285618949 0.0 7 0.0 0.0 0.0 2.0604767027770268 0.0 8 0.0 0.0 0.0 2.5251842433796075 0.0 9 0.0 0.0 0.0 3.094023092987533 0.0 10 0.0 0.0 0.0 4.052059901391678 0.0 11 0.0 0.0 0.0 4.86080347948153 0.0 12 0.0 0.0 0.0 5.298759053958428 0.0 13 0.0 0.0 0.0 5.48515985018899 0.0 14 0.0 0.0 0.0 5.658328491131579 0.0 15 0.0 0.0 0.0 5.8081970325453485 0.0 16 0.0 0.0 0.0 6.131521870451446 0.0 17 0.0 0.0 0.0 6.554087873017334 0.0 18 0.0 0.0 0.0 7.113290261709019 0.0 19 0.0 0.0 0.0 7.348304845845391 0.0 20 1.4382777486926054E-4 0.0 0.0 7.592092924248788 0.0 21 1.4382777486926054E-4 0.0 0.0 7.861194691029174 0.0 22 1.4382777486926054E-4 0.0 0.0 8.16323301825462 0.0 23 1.4382777486926054E-4 0.0 0.0 8.485551061736635 0.0 24 1.4382777486926054E-4 0.0 0.0 8.722147751396568 0.0 25 1.4382777486926054E-4 0.0 0.0 8.918616491867978 0.0 26 1.4382777486926054E-4 0.0 0.0 9.11062657131844 0.0 27 1.4382777486926054E-4 0.0 0.0 9.348517710952198 0.0 28 1.4382777486926054E-4 0.0 0.0 9.56397171770635 0.0 29 1.4382777486926054E-4 0.0 0.0 9.819985156973633 0.0 30 1.4382777486926054E-4 0.0 0.0 10.121592000874474 0.0 31 1.4382777486926054E-4 0.0 0.0 10.345100363021304 0.0 32 1.4382777486926054E-4 0.0 0.0 10.549191975560785 0.0 33 1.4382777486926054E-4 0.0 0.0 10.765508948964152 0.0 34 1.4382777486926054E-4 0.0 0.0 10.989161138885853 0.0 35 1.4382777486926054E-4 0.0 0.0 11.231654767315426 0.0 36 1.4382777486926054E-4 0.0 0.0 11.482058923362809 0.0 37 1.4382777486926054E-4 0.0 0.0 11.74627054579764 0.0 38 1.4382777486926054E-4 0.0 0.0 12.022995184646097 0.0 39 1.4382777486926054E-4 0.0 0.0 12.322588439698768 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTTAGC 25 3.889209E-5 45.000004 31 TCGTTCC 25 3.889209E-5 45.000004 27 TCCGCGC 25 3.889209E-5 45.000004 23 CGTATCC 45 3.8380676E-10 45.000004 29 CGTGAGT 25 3.889209E-5 45.000004 34 GACCGTA 50 2.1827873E-11 45.000004 26 TAGAACG 25 3.889209E-5 45.000004 1 TGAACGG 85 0.0 45.000004 2 GCACGAG 25 3.889209E-5 45.000004 1 CGTCTAA 25 3.889209E-5 45.000004 20 CGATTCG 25 3.889209E-5 45.000004 11 CCGTATC 45 3.8380676E-10 45.000004 28 ACCTAGC 25 3.889209E-5 45.000004 11 ACGGATA 25 3.889209E-5 45.000004 5 CGAATTG 25 3.889209E-5 45.000004 1 GAACGAA 25 3.889209E-5 45.000004 12 CATACCG 50 2.1827873E-11 45.000004 1 TCATCGG 45 3.8380676E-10 45.000004 2 CGGCTAG 45 3.8380676E-10 45.000004 1 ACCGTAT 45 3.8380676E-10 45.000004 27 >>END_MODULE