##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548559_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	692255
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.673158012582068	31.0	31.0	34.0	30.0	34.0
2	31.879256921221227	33.0	31.0	34.0	30.0	34.0
3	32.04947309878585	33.0	31.0	34.0	30.0	34.0
4	35.782421217614896	37.0	35.0	37.0	35.0	37.0
5	35.54663238257579	37.0	35.0	37.0	33.0	37.0
6	32.73396219601158	37.0	35.0	37.0	26.0	37.0
7	34.2834735754888	37.0	35.0	37.0	28.0	37.0
8	35.41357158850423	37.0	35.0	37.0	32.0	37.0
9	37.28414962694383	39.0	37.0	39.0	34.0	39.0
10	36.71107467623925	38.0	37.0	39.0	32.0	39.0
11	36.776533213916835	39.0	37.0	39.0	32.0	39.0
12	36.831785974821415	39.0	37.0	39.0	33.0	39.0
13	36.836966146867844	39.0	37.0	39.0	33.0	39.0
14	37.95022210023763	40.0	37.0	41.0	33.0	41.0
15	38.02314320589956	40.0	37.0	41.0	33.0	41.0
16	37.974548396183486	40.0	37.0	41.0	33.0	41.0
17	37.96210789376747	40.0	37.0	41.0	33.0	41.0
18	37.81859574867643	39.0	37.0	41.0	33.0	41.0
19	37.7826306780016	39.0	36.0	41.0	33.0	41.0
20	37.66509306541665	39.0	36.0	41.0	33.0	41.0
21	37.60830329863995	39.0	36.0	41.0	33.0	41.0
22	37.58631862536204	39.0	36.0	41.0	33.0	41.0
23	37.41269618854324	39.0	36.0	41.0	32.0	41.0
24	37.3036121082549	39.0	36.0	41.0	32.0	41.0
25	37.34804082310709	39.0	36.0	41.0	32.0	41.0
26	37.26491827433532	39.0	35.0	41.0	32.0	41.0
27	37.17050075477967	39.0	35.0	41.0	32.0	41.0
28	37.14241283919943	39.0	35.0	41.0	32.0	41.0
29	36.951545312059864	39.0	35.0	41.0	31.0	41.0
30	36.97601172978165	39.0	35.0	41.0	31.0	41.0
31	36.579170970234955	39.0	35.0	40.0	30.0	41.0
32	36.67371705513142	39.0	35.0	40.0	30.0	41.0
33	36.65796130038786	39.0	35.0	40.0	31.0	41.0
34	36.55960592556211	39.0	35.0	40.0	30.0	41.0
35	36.55193678629985	39.0	35.0	40.0	30.0	41.0
36	36.474088305609925	39.0	35.0	40.0	30.0	41.0
37	36.462544871470776	39.0	35.0	40.0	30.0	41.0
38	36.334353670251566	39.0	35.0	40.0	30.0	41.0
39	36.24739005135391	39.0	35.0	40.0	30.0	41.0
40	36.12727535373526	39.0	35.0	40.0	30.0	41.0
41	36.094192169070645	38.0	35.0	40.0	30.0	41.0
42	35.988894265841346	38.0	35.0	40.0	29.0	41.0
43	35.928678016049	38.0	35.0	40.0	29.0	41.0
44	35.767799438068344	38.0	35.0	40.0	29.0	41.0
45	35.61063625398155	38.0	35.0	40.0	28.0	41.0
46	35.37157405869225	38.0	34.0	40.0	27.0	41.0
47	35.36341666004579	38.0	34.0	40.0	27.0	41.0
48	35.35333944861359	38.0	34.0	40.0	27.0	41.0
49	35.22362568706618	38.0	34.0	40.0	27.0	41.0
50	35.07964261724364	38.0	34.0	40.0	26.0	41.0
51	34.05796996771421	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	12.0
11	3.0
12	7.0
13	13.0
14	22.0
15	43.0
16	74.0
17	183.0
18	323.0
19	718.0
20	1199.0
21	1752.0
22	2533.0
23	3311.0
24	4110.0
25	4830.0
26	5594.0
27	6748.0
28	8221.0
29	10404.0
30	13450.0
31	17344.0
32	23141.0
33	31268.0
34	44877.0
35	62072.0
36	70423.0
37	96297.0
38	135725.0
39	147530.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.23596073701165	32.9564972445125	19.99451069331388	25.813031325161973
2	22.83883828935869	32.56791211331085	19.335649435540372	25.25760016179009
3	20.240229395237304	34.78544755906422	20.045359007880045	24.928964037818435
4	19.627882788856706	36.00725166304324	18.319260965973523	26.04560458212653
5	17.068421318733705	37.97733494160389	16.471242533459492	28.483001206202918
6	16.94245617583116	45.732136279261255	15.827115730475041	21.498291814432545
7	84.59411632996512	9.591263335042722	3.0942355057023785	2.7203848292897845
8	86.77886039104087	8.20810250557959	2.404749694837885	2.60828740854165
9	79.82983149273028	12.613993398386434	4.550057421037046	3.0061176878462414
10	29.273461369004195	46.6969541570664	11.582437107713197	12.447147366216207
11	18.82442163653567	31.860369372557802	32.362352023459565	16.952856967446966
12	23.678991123213265	26.884312861590022	32.434435287574665	17.002260727622044
13	23.326519851788717	27.272031260157025	30.5446692331583	18.856779654895956
14	18.514275808769888	32.861012199261836	28.866241486157556	19.75847050581072
15	18.157181963293873	34.48902499801374	29.189099392564877	18.16469364612751
16	19.97616485254711	32.29662479866523	28.275129829325902	19.45208051946176
17	19.945106933138803	31.87120353049093	28.228615177932987	19.95507435843728
18	19.52344150638132	32.180915991939386	28.64840268398206	19.647239817697233
19	20.031924652042964	34.651392911571605	27.671595004730914	17.64508743165452
20	21.004687578999068	32.85754526872323	27.914857964189498	18.222909188088206
21	21.37954944348542	31.540689485810862	29.032943062888677	18.046818007815038
22	21.190890640009822	29.61697640320402	27.65570490642899	21.536428050357166
23	19.04327162678493	34.06562610598696	27.239673241796737	19.65142902543138
24	17.391134769701917	34.331713024824666	28.91434514738066	19.362807058092756
25	20.40447522950358	33.0987858520343	25.879191916273626	20.6175470021885
26	20.774570064499354	33.82886364128825	25.011448093549344	20.38511820066305
27	17.957689001885143	32.599114488158264	27.886833609002466	21.556362900954127
28	19.249409538392644	33.031325161970656	28.03071122635445	19.688554073282244
29	19.9313836664235	32.51287459101054	26.882723851759827	20.673017890806133
30	20.12712078641541	32.23234212826199	27.62464698702068	20.015890098301924
31	20.32675820326325	33.503983358733414	25.41158965987967	20.75766877812367
32	20.643260070349797	32.1794714375483	27.228116806668062	19.949151685433836
33	20.422243248513915	32.93901813638038	25.707145488295495	20.931593126810206
34	17.99192494095384	33.51712880369228	28.243349632722047	20.247596622631832
35	19.381152898859526	31.853435511480594	28.080981719164182	20.6844298704957
36	19.398920917869862	34.2174487724899	26.39937595250305	19.984254357137182
37	20.540118886826384	32.872279723512285	26.162035666047917	20.42556572361341
38	19.384908740276344	32.57383478631429	28.04992379975587	19.991332673653496
39	19.7392579324093	30.649832792829233	27.373727889289352	22.237181385472116
40	21.04831312160981	31.745382843027496	26.397642487233753	20.80866154812894
41	19.18209330376812	30.098735292630607	29.105893059638426	21.613278343962847
42	20.47453611747116	32.04772807708142	25.485984211020508	21.99175159442691
43	18.643130060454602	32.87502437685535	26.96304107590411	21.51880448678594
44	20.884356198221752	31.784963633343207	26.857444149915853	20.473236018519188
45	21.668171410824048	30.186708655047635	27.566286989620874	20.578832944507443
46	19.312247654404807	31.516709882918864	27.93479281478646	21.236249647889867
47	19.287112407999942	32.63609508057002	27.867476580161938	20.2093159312681
48	19.43705715379448	30.782876252248087	28.769745252833133	21.010321341124296
49	20.39248542805758	31.377021473301024	27.346859177615183	20.883633921026213
50	18.374587399152045	30.424048941502768	28.860318813154112	22.34104484619107
51	18.510808878231288	30.858137536023577	28.186867556030652	22.444186029714484
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17081.0
1	13383.5
2	9686.0
3	6234.0
4	2782.0
5	2261.5
6	1741.0
7	1893.5
8	2046.0
9	2441.5
10	2837.0
11	3062.0
12	3287.0
13	3527.0
14	3767.0
15	3838.0
16	3909.0
17	3503.5
18	3098.0
19	3667.5
20	4237.0
21	4229.5
22	4222.0
23	5057.0
24	5892.0
25	7205.5
26	9899.0
27	11279.0
28	13023.0
29	14767.0
30	15850.5
31	16934.0
32	19744.0
33	22554.0
34	25401.5
35	28249.0
36	30979.0
37	33709.0
38	32756.0
39	31803.0
40	32574.5
41	33346.0
42	36116.5
43	38887.0
44	44077.5
45	49268.0
46	47934.0
47	46600.0
48	49603.5
49	52607.0
50	51033.5
51	49460.0
52	46266.5
53	43073.0
54	37766.5
55	32460.0
56	29334.5
57	26209.0
58	23841.5
59	21474.0
60	20016.0
61	18558.0
62	16541.5
63	14525.0
64	12486.5
65	10448.0
66	9322.0
67	8196.0
68	6475.0
69	4754.0
70	3786.5
71	2819.0
72	2813.5
73	2808.0
74	2292.0
75	1530.5
76	1285.0
77	969.5
78	654.0
79	508.0
80	362.0
81	221.0
82	80.0
83	99.0
84	118.0
85	87.5
86	57.0
87	37.5
88	18.0
89	12.5
90	7.0
91	6.5
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	692255.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.869103367823932
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.09843525237025	18.924998524208526
2	9.147882190406959	4.5499925557981244
3	3.5000010684260783	2.611256650745471
4	1.9122336984130444	1.9022215003708103
5	1.2034097746482733	1.4963861039788806
6	0.8458624099361073	1.2621503824594629
7	0.6619256975816183	1.1523049016483358
8	0.5165213838119728	1.0276338948569128
9	0.4477890646814841	1.0022501282890524
>10	4.190319087884868	22.93980318565189
>50	0.8966463620368837	15.939327722299309
>100	0.5731020624413874	23.0991818530387
>500	0.0023487789444319156	0.3731068183569068
>1k	0.0029359736805398945	1.5480647285329039
>5k	0.0	0.0
>10k+	5.871947361079789E-4	2.1713210497647153
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14869	2.1479079241031123	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3517	0.5080497793443167	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2983	0.4309105748604199	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1530	0.22101682183588417	No Hit
CTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGCT	1407	0.20324880282554836	No Hit
GGCCTCAGGGATTTTTTTTTCTTTTAGAATGTAAGCTCGTCTAGAGCAGGG	1164	0.16814613112220209	No Hit
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	787	0.11368643057832735	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2416739496283884	0.0
2	0.0	0.0	0.0	0.331091866436501	0.0
3	0.0	0.0	0.0	0.4329329510079378	0.0
4	0.0	0.0	0.0	0.9125250088478957	0.0
5	0.0	0.0	0.0	0.9958757972134545	0.0
6	0.0	0.0	0.0	1.5507291388288997	0.0
7	0.0	0.0	0.0	1.970805555756188	0.0
8	0.0	0.0	0.0	2.401716130616608	0.0
9	0.0	0.0	0.0	2.9336010574138145	0.0
10	0.0	0.0	0.0	3.858115867707709	0.0
11	0.0	0.0	0.0	4.652765238243133	0.0
12	0.0	0.0	0.0	5.065618883215	0.0
13	0.0	0.0	0.0	5.242143429805491	0.0
14	0.0	0.0	0.0	5.417512332883114	0.0
15	0.0	0.0	0.0	5.559367574087584	0.0
16	0.0	0.0	0.0	5.869368946414255	0.0
17	0.0	0.0	0.0	6.277888928212869	0.0
18	0.0	0.0	0.0	6.813529696426895	0.0
19	0.0	0.0	0.0	7.039313547753356	0.0
20	0.0	0.0	0.0	7.284309972481239	0.0
21	0.0	0.0	0.0	7.5295953080873375	0.0
22	0.0	0.0	0.0	7.829773710554637	0.0
23	0.0	0.0	0.0	8.146420033080295	0.0
24	0.0	0.0	0.0	8.368881409307264	0.0
25	0.0	0.0	0.0	8.550606351705657	0.0
26	0.0	0.0	0.0	8.734498125690678	0.0
27	0.0	0.0	0.0	8.965193461946827	0.0
28	0.0	0.0	0.0	9.17696513567977	0.0
29	0.0	0.0	0.0	9.420661461455678	0.0
30	0.0	0.0	0.0	9.711450260380929	0.0
31	0.0	0.0	0.0	9.934345002925223	0.0
32	0.0	0.0	0.0	10.152183805100721	0.0
33	0.0	0.0	0.0	10.366844587615835	0.0
34	0.0	0.0	0.0	10.584827845230443	0.0
35	0.0	0.0	0.0	10.838636051743938	0.0
36	0.0	0.0	0.0	11.082187922080736	0.0
37	0.0	0.0	0.0	11.345530187575388	0.0
38	0.0	0.0	0.0	11.614072848877942	0.0
39	0.0	0.0	0.0	11.92999689420806	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACT	30	2.1642463E-6	45.000004	42
GACACGT	30	2.1642463E-6	45.000004	23
ATGCTCG	55	1.8189894E-12	45.000004	1
GCTTGCG	30	2.1642463E-6	45.000004	22
CCTCGCG	30	2.1642463E-6	45.000004	1
TCGTATA	30	2.1642463E-6	45.000004	35
TCATCGG	30	2.1642463E-6	45.000004	2
CGCACTA	30	2.1642463E-6	45.000004	43
TACCGTT	30	2.1642463E-6	45.000004	27
GATACGA	20	7.0313405E-4	45.0	8
AGCCGAT	20	7.0313405E-4	45.0	37
AATCCCG	20	7.0313405E-4	45.0	1
GTCGCGC	20	7.0313405E-4	45.0	43
GGTACGT	25	3.889193E-5	45.0	13
ACACGTC	20	7.0313405E-4	45.0	19
GTCGATC	20	7.0313405E-4	45.0	25
ATGATCG	20	7.0313405E-4	45.0	32
CTCGTAC	40	6.8084773E-9	45.0	30
GATCGCC	25	3.889193E-5	45.0	9
CCGATTA	25	3.889193E-5	45.0	42
>>END_MODULE