SRR3548558_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548558_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	282989
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18992	6.711214923548265	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4155	1.4682549498390396	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGT	3766	1.33079377643654	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3623	1.2802617769595284	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCC	3170	1.1201848835113732	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCT	2655	0.9381990112689892	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1899	0.6710508182296838	No Hit
CTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT	1792	0.6332401612783536	Illumina Single End Adapter 2 (95% over 22bp)
AATGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTT	902	0.318740304393457	No Hit
AAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCT	682	0.24099876673651624	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTT	644	0.22757068295940833	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCT	617	0.2180296760651474	No Hit
AACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTG	513	0.18127913099095724	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTAT	487	0.17209149472240973	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	479	0.16926452971670278	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTACAGGTC	446	0.15760329906816167	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	410	0.14488195654248046	No Hit
TGGTAAGGGGCATGTTTAGACCTGGCTTTGAGCCAACCCAACCTAAGAACT	410	0.14488195654248046	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCT	398	0.14064150903392003	No Hit
ATGATGGGGTCCATAAGGTGAGCCACGAGGGCTGTTGTTTTTCTTAGTTTT	345	0.1219128658711116	No Hit
CAACCAGGGATTGATTTTAAAGATTTTTTTTTAAATTTCACATTTTTTTTT	328	0.11590556523398436	No Hit
ATTTACGGGATTATGTTTTATAGCTAATGATGTGTAAAGTCTAAAGATTTT	328	0.11590556523398436	No Hit
TCAGAAGGGCTGTACTGTGAGATTGCCCGGTGCAGCAGCAGTTGTATTCTT	296	0.10459770521115662	No Hit
TGGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCC	295	0.10424433458544324	No Hit
TGACAAGGGGAAAAGAGAAAGTTTGTTTTTTGGGGTTTTTTTGTTTGTTTG	293	0.10353759333401652	No Hit
GCTAAAGGGTGTATGATTTCAGCATGCTCTTTTTTTTTCCTCTAGTTTTTC	285	0.10071062832830958	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCATTCT	35	1.2075907E-7	45.000004	28
ATAGCGG	35	1.2075907E-7	45.000004	2
ACACAAG	35	1.2075907E-7	45.000004	1
ACCTGAT	35	1.2075907E-7	45.000004	25
ATACTCC	35	1.2075907E-7	45.000004	13
TACGAGG	35	1.2075907E-7	45.000004	2
CGGGATC	35	1.2075907E-7	45.000004	6
CATACTC	35	1.2075907E-7	45.000004	12
CGTACTC	35	1.2075907E-7	45.000004	45
TACTCCC	35	1.2075907E-7	45.000004	14
GGTGATT	65	0.0	45.000004	8
TATAGCG	35	1.2075907E-7	45.000004	21
TTGCCTC	35	1.2075907E-7	45.000004	40
ACACTAG	35	1.2075907E-7	45.000004	43
TCATAAC	35	1.2075907E-7	45.000004	28
AATAGTG	70	0.0	45.000004	33
TTCATAA	35	1.2075907E-7	45.000004	27
AGTAGTG	35	1.2075907E-7	45.000004	21
TGAATTA	35	1.2075907E-7	45.000004	21
GCCGTAC	35	1.2075907E-7	45.000004	43

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content