##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548558_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	282989
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.510807840587443	31.0	31.0	34.0	30.0	34.0
2	31.704889589347996	31.0	31.0	34.0	30.0	34.0
3	31.88337355868956	31.0	31.0	34.0	30.0	34.0
4	35.59832714345787	37.0	35.0	37.0	33.0	37.0
5	35.30528748467255	37.0	35.0	37.0	33.0	37.0
6	33.96445798246575	37.0	35.0	37.0	31.0	37.0
7	34.611970783316664	37.0	35.0	37.0	31.0	37.0
8	35.26182996512232	37.0	35.0	37.0	32.0	37.0
9	36.908060030601895	39.0	37.0	39.0	33.0	39.0
10	36.53871705260629	38.0	35.0	39.0	32.0	39.0
11	36.56793020223401	38.0	35.0	39.0	32.0	39.0
12	36.45803900504966	38.0	35.0	39.0	32.0	39.0
13	36.46430779994982	38.0	35.0	39.0	32.0	39.0
14	37.52498153638481	39.0	36.0	41.0	32.0	41.0
15	37.61421115308369	39.0	36.0	41.0	32.0	41.0
16	37.433066302930506	39.0	36.0	41.0	32.0	41.0
17	37.31433730639707	39.0	36.0	40.0	32.0	41.0
18	36.90101382032517	38.0	36.0	40.0	31.0	41.0
19	36.538091586598775	38.0	35.0	40.0	31.0	41.0
20	36.48269367360569	38.0	35.0	40.0	31.0	41.0
21	36.452176586369085	38.0	35.0	40.0	31.0	41.0
22	36.41563806367032	38.0	35.0	40.0	31.0	41.0
23	36.14205852524303	38.0	35.0	40.0	30.0	41.0
24	35.93899056147059	38.0	34.0	40.0	30.0	41.0
25	36.01372138139645	38.0	35.0	40.0	30.0	41.0
26	35.86210064702161	38.0	34.0	40.0	30.0	41.0
27	35.847403962698195	38.0	35.0	40.0	30.0	41.0
28	35.74354480209478	38.0	34.0	40.0	29.0	41.0
29	35.58380361074105	38.0	34.0	40.0	29.0	41.0
30	35.55643505577955	38.0	34.0	40.0	29.0	41.0
31	35.36825459646841	38.0	34.0	40.0	27.0	41.0
32	35.25501697945857	38.0	34.0	40.0	27.0	41.0
33	35.06106597782953	37.0	34.0	40.0	26.0	41.0
34	35.03228747407143	37.0	34.0	40.0	25.0	41.0
35	34.86640823494906	37.0	34.0	40.0	25.0	41.0
36	34.66693051673386	37.0	33.0	40.0	24.0	41.0
37	34.65814925668489	37.0	34.0	40.0	24.0	41.0
38	34.416224658909	37.0	33.0	40.0	24.0	41.0
39	34.28017696800936	37.0	33.0	40.0	23.0	41.0
40	34.04739760202693	36.0	33.0	40.0	22.0	41.0
41	33.879380470619	36.0	33.0	40.0	22.0	41.0
42	33.77042217188654	36.0	33.0	40.0	21.0	41.0
43	33.6854259352837	36.0	33.0	40.0	21.0	41.0
44	33.610044913406526	36.0	33.0	40.0	21.0	41.0
45	33.376873306029566	35.0	33.0	40.0	20.0	41.0
46	33.23354971394648	35.0	32.0	39.0	20.0	41.0
47	33.11983858029817	35.0	32.0	39.0	20.0	41.0
48	33.022976158083885	35.0	32.0	39.0	20.0	41.0
49	32.84443918314846	35.0	32.0	39.0	18.0	41.0
50	32.66549583199347	35.0	31.0	39.0	18.0	40.0
51	31.498245514843333	35.0	29.0	38.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	2.0
12	6.0
13	9.0
14	19.0
15	36.0
16	93.0
17	228.0
18	381.0
19	678.0
20	1163.0
21	1771.0
22	2322.0
23	2837.0
24	3329.0
25	3510.0
26	3920.0
27	4302.0
28	5054.0
29	6349.0
30	7662.0
31	9771.0
32	12415.0
33	16064.0
34	22416.0
35	36153.0
36	28623.0
37	34278.0
38	43404.0
39	36185.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.376686019597933	40.282131107569555	16.229252727137805	25.11193014569471
2	19.129365452367406	44.03351367014265	15.455371056825532	21.38174982066441
3	16.469544752622895	40.34361759644368	21.763743467060557	21.42309418387287
4	19.896533080791126	40.71253652968843	15.582937852708056	23.807992536812385
5	14.770538784193024	47.95840120994102	14.457452409810982	22.813607596054972
6	15.564915950796673	46.793691627589766	19.160815438055895	18.480576983557665
7	66.12942552537378	23.31009332518225	5.791391184816371	4.769089964627601
8	67.36410249161628	19.037842460307644	4.645410245627922	8.952644802448152
9	64.36327913805837	21.241108311630487	8.314104081784098	6.081508468527045
10	32.126690436730755	38.32375109986607	12.889546943520772	16.6600115198824
11	21.747135047652026	37.53432112202241	21.04216064935386	19.676383180971698
12	20.35556152359279	32.35850156719873	29.056606440533024	18.22933046867546
13	21.679641258140776	35.543077646127585	23.240832682542433	19.536448413189206
14	15.083271787949354	36.37208513405115	28.73927961864242	19.80536345935708
15	16.88369512595896	38.13081073822657	23.41681125414769	21.568682881666778
16	16.849418175264763	37.4099346617713	26.36922283198287	19.371424330981064
17	17.64697567749983	35.12892727279152	25.532441190293614	21.69165585941503
18	18.719455526539903	36.82121919933284	22.9835788670231	21.47574640710416
19	17.964302499390435	39.67115329571114	25.515479400259373	16.84906480463905
20	19.810664018742777	40.05986098399584	22.175420246016632	17.954054751244747
21	18.104590637798644	38.082398962503845	22.67084586326677	21.142164536430744
22	17.945220485601915	38.22162698903491	21.807914795274726	22.02523773008845
23	18.116251868447183	38.841085695910444	20.26085819590161	22.781804239740765
24	15.596719307110876	42.04050334111927	21.376449261278708	20.98632809049115
25	18.62263197509444	36.93359105830969	24.18751258882854	20.256264377767334
26	20.11420938623056	38.17851577269788	21.0114174049168	20.695857436154764
27	15.575163698942362	36.52474124435932	25.044436356183457	22.855658700514862
28	18.497538773591906	35.6384877150702	26.01867917127521	19.84529434006269
29	19.66472195032316	38.044588305552516	21.73900752326062	20.55168222086371
30	19.424783295463783	36.2915166313885	22.768022785337948	21.515677287809773
31	18.94843969200216	39.17608104908671	18.804971217962535	23.070508040948585
32	18.7537324772341	36.691178809070315	21.077144341299487	23.477944372396102
33	18.58517468876882	37.87285018145582	23.20160854308825	20.340366586687114
34	19.6922848591288	34.2083967928082	21.39977172257579	24.69954662548721
35	18.35301018767514	34.466710720204674	24.90344147652382	22.276837615596364
36	19.753417977377214	36.15052175172887	23.92531158454922	20.1707486863447
37	19.51913325252925	35.79962472039549	22.325956132570525	22.355285894504735
38	21.453837428309935	37.86330917456156	21.62663566428377	19.05621773284474
39	21.62133510489807	33.28892642470202	22.57225545869274	22.517483011707167
40	23.384301156582058	34.846937513472255	21.17502800462209	20.593733325323598
41	20.975726971719748	31.755651279731723	24.798843771312665	22.469777977235864
42	22.44398192155879	32.34224651841591	23.545438161907352	21.668333398117948
43	19.49475067935503	32.52635261441257	24.214368756382758	23.76452794984964
44	20.248136853375925	30.593061921134744	24.593535437773202	24.565265787716132
45	22.154218008473826	33.98435981610592	24.535936025781922	19.325486149638323
46	18.687652170225697	31.400160430264073	28.5615341939086	21.35065320560163
47	20.059436939244986	35.29430472562537	23.625299923318575	21.020958411811062
48	22.35281230012474	29.976430179264916	27.150525285435123	20.520232235175218
49	20.94569046853411	29.802571831413943	25.505585022739403	23.746152677312544
50	19.28202156267558	29.72058984624844	26.332472286908676	24.6649163041673
51	20.787380428214526	29.209969292092623	28.39403651732046	21.60861376237239
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21563.0
1	16062.0
2	10561.0
3	6429.0
4	2297.0
5	1889.5
6	1482.0
7	1483.0
8	1484.0
9	1501.0
10	1518.0
11	1641.0
12	1764.0
13	1736.5
14	1709.0
15	1895.0
16	2081.0
17	2000.0
18	1919.0
19	1969.0
20	2019.0
21	1844.0
22	1669.0
23	1574.0
24	1479.0
25	1561.5
26	1775.5
27	1907.0
28	2473.5
29	3040.0
30	2549.0
31	2058.0
32	2734.0
33	3410.0
34	4307.0
35	5204.0
36	5256.5
37	5309.0
38	5554.5
39	5800.0
40	6590.5
41	7381.0
42	8657.5
43	9934.0
44	16326.5
45	22719.0
46	22588.0
47	22457.0
48	22935.0
49	23413.0
50	22146.5
51	20880.0
52	18590.5
53	16301.0
54	15863.0
55	15425.0
56	14232.5
57	13040.0
58	11716.0
59	10392.0
60	9861.0
61	9330.0
62	9134.0
63	8938.0
64	7591.0
65	6244.0
66	5445.5
67	4647.0
68	4468.0
69	4289.0
70	3352.0
71	2415.0
72	2163.5
73	1912.0
74	1628.0
75	1040.5
76	737.0
77	651.5
78	566.0
79	460.5
80	355.0
81	285.0
82	215.0
83	134.0
84	53.0
85	39.0
86	25.0
87	18.0
88	11.0
89	10.5
90	10.0
91	15.0
92	20.0
93	19.0
94	18.0
95	9.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	282989.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.099325415475512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.18664956362082	22.812900854803544
2	8.228325662114239	4.62420800808512
3	2.9729117935561757	2.5061044775591985
4	1.5858044719434594	1.782401436098223
5	0.9054553685957897	1.272134252568121
6	0.5860308357856083	0.9880242694945741
7	0.4388943383887925	0.8632844386177554
8	0.34583364772755854	0.7774153765694073
9	0.266606302975427	0.6742311538611041
>10	2.3177142282250562	14.61788267388485
>50	0.7105309489675294	14.62212312139341
>100	0.4388943383887925	19.119471074847432
>500	0.0062878845041374285	1.1866185611454863
>1k	0.0088030383057924	7.441985377523508
>5k	0.0	0.0
>10k+	0.0012575769008274856	6.711214923548265
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18992	6.711214923548265	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4155	1.4682549498390396	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGT	3766	1.33079377643654	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3623	1.2802617769595284	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCC	3170	1.1201848835113732	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCT	2655	0.9381990112689892	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1899	0.6710508182296838	No Hit
CTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT	1792	0.6332401612783536	Illumina Single End Adapter 2 (95% over 22bp)
AATGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTT	902	0.318740304393457	No Hit
AAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCT	682	0.24099876673651624	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTT	644	0.22757068295940833	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCT	617	0.2180296760651474	No Hit
AACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTG	513	0.18127913099095724	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTAT	487	0.17209149472240973	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	479	0.16926452971670278	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTACAGGTC	446	0.15760329906816167	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	410	0.14488195654248046	No Hit
TGGTAAGGGGCATGTTTAGACCTGGCTTTGAGCCAACCCAACCTAAGAACT	410	0.14488195654248046	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCT	398	0.14064150903392003	No Hit
ATGATGGGGTCCATAAGGTGAGCCACGAGGGCTGTTGTTTTTCTTAGTTTT	345	0.1219128658711116	No Hit
CAACCAGGGATTGATTTTAAAGATTTTTTTTTAAATTTCACATTTTTTTTT	328	0.11590556523398436	No Hit
ATTTACGGGATTATGTTTTATAGCTAATGATGTGTAAAGTCTAAAGATTTT	328	0.11590556523398436	No Hit
TCAGAAGGGCTGTACTGTGAGATTGCCCGGTGCAGCAGCAGTTGTATTCTT	296	0.10459770521115662	No Hit
TGGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCC	295	0.10424433458544324	No Hit
TGACAAGGGGAAAAGAGAAAGTTTGTTTTTTGGGGTTTTTTTGTTTGTTTG	293	0.10353759333401652	No Hit
GCTAAAGGGTGTATGATTTCAGCATGCTCTTTTTTTTTCCTCTAGTTTTTC	285	0.10071062832830958	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7537395446466117	0.0
2	0.0	0.0	0.0	0.9608147313146448	0.0
3	0.0	0.0	0.0	1.3668375802593034	0.0
4	0.0	0.0	0.0	3.397658566234023	0.0
5	0.0	0.0	0.0	3.664100018021902	0.0
6	0.0	0.0	0.0	5.0722819614896695	0.0
7	0.0	0.0	0.0	6.167730901201106	0.0
8	0.0	0.0	0.0	6.886840124527809	0.0
9	0.0	0.0	0.0	9.175621667273287	0.0
10	0.0	0.0	0.0	10.843531020640379	0.0
11	0.0	0.0	0.0	13.178957485980021	0.0
12	0.0	0.0	0.0	13.877217842389634	0.0
13	0.0	0.0	0.0	14.23164857998014	0.0
14	0.0	0.0	0.0	14.693503987787512	0.0
15	0.0	0.0	0.0	14.985034754001038	0.0
16	0.0	0.0	0.0	15.464205322468365	0.0
17	0.0	0.0	0.0	16.07482976370106	0.0
18	0.0	0.0	0.0	17.341663456883484	0.0
19	0.0	0.0	0.0	17.666057691288355	3.5337062571336696E-4
20	0.0	0.0	0.0	18.045224372678796	3.5337062571336696E-4
21	0.0	0.0	0.0	18.335695027015184	3.5337062571336696E-4
22	0.0	0.0	0.0	18.623338716345867	3.5337062571336696E-4
23	0.0	0.0	0.0	18.935011608225054	3.5337062571336696E-4
24	0.0	0.0	0.0	19.13890645926167	3.5337062571336696E-4
25	0.0	0.0	0.0	19.326546261515464	3.5337062571336696E-4
26	0.0	0.0	0.0	19.495810791232167	3.5337062571336696E-4
27	0.0	0.0	0.0	19.74281685860581	3.5337062571336696E-4
28	0.0	0.0	0.0	19.952012269028124	3.5337062571336696E-4
29	0.0	0.0	0.0	20.15095993130475	3.5337062571336696E-4
30	0.0	0.0	0.0	20.431536208121162	3.5337062571336696E-4
31	0.0	0.0	0.0	20.60221422034072	3.5337062571336696E-4
32	0.0	0.0	0.0	20.800808511991633	3.5337062571336696E-4
33	0.0	0.0	0.0	20.975726971719748	3.5337062571336696E-4
34	0.0	0.0	0.0	21.15241228457643	3.5337062571336696E-4
35	0.0	0.0	0.0	21.351713317478772	3.5337062571336696E-4
36	0.0	0.0	0.0	21.533699189721155	3.5337062571336696E-4
37	0.0	0.0	0.0	21.7252260688578	3.5337062571336696E-4
38	0.0	0.0	0.0	21.898731046083064	3.5337062571336696E-4
39	0.0	0.0	0.0	22.094498372728268	3.5337062571336696E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCATTCT	35	1.2075907E-7	45.000004	28
ATAGCGG	35	1.2075907E-7	45.000004	2
ACACAAG	35	1.2075907E-7	45.000004	1
ACCTGAT	35	1.2075907E-7	45.000004	25
ATACTCC	35	1.2075907E-7	45.000004	13
TACGAGG	35	1.2075907E-7	45.000004	2
CGGGATC	35	1.2075907E-7	45.000004	6
CATACTC	35	1.2075907E-7	45.000004	12
CGTACTC	35	1.2075907E-7	45.000004	45
TACTCCC	35	1.2075907E-7	45.000004	14
GGTGATT	65	0.0	45.000004	8
TATAGCG	35	1.2075907E-7	45.000004	21
TTGCCTC	35	1.2075907E-7	45.000004	40
ACACTAG	35	1.2075907E-7	45.000004	43
TCATAAC	35	1.2075907E-7	45.000004	28
AATAGTG	70	0.0	45.000004	33
TTCATAA	35	1.2075907E-7	45.000004	27
AGTAGTG	35	1.2075907E-7	45.000004	21
TGAATTA	35	1.2075907E-7	45.000004	21
GCCGTAC	35	1.2075907E-7	45.000004	43
>>END_MODULE