SRR3548557_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548557_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	280033
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18892	6.746347751872101	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4169	1.4887531112404608	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3548	1.2669935329050503	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGT	3325	1.1873600611356518	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCC	2830	1.0105951798538029	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCT	2404	0.858470251720333	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1866	0.6663500373170305	No Hit
CTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT	1756	0.6270689525877308	Illumina Single End Adapter 2 (95% over 22bp)
AATGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTT	767	0.2738962907942992	No Hit
AAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCT	676	0.24140012070006037	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCT	561	0.20033353211942878	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTT	540	0.19283441594383519	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTAT	503	0.17962168744397983	No Hit
AACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTG	494	0.17640778051158254	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	473	0.16890866433598897	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTACAGGTC	461	0.16462345509279264	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	386	0.13784089732281551	No Hit
TGGTAAGGGGCATGTTTAGACCTGGCTTTGAGCCAACCCAACCTAAGAACT	384	0.13712669578228281	No Hit
CAACCAGGGATTGATTTTAAAGATTTTTTTTTAAATTTCACATTTTTTTTT	348	0.12427106805269379	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCT	310	0.11070123878257207	No Hit
ATGATGGGGTCCATAAGGTGAGCCACGAGGGCTGTTGTTTTTCTTAGTTTT	307	0.10962993647177298	No Hit
GCAGCAGGGTGATTTTAGTGCAATAATCTCTGATTATTTAGAAGGAAAACG	300	0.10713023107990845	No Hit
ATTTACGGGATTATGTTTTATAGCTAATGATGTGTAAAGTCTAAAGATTTT	299	0.10677313030964207	No Hit
ACTCAAGGGCGTTATTGTTTAGCTAAGACTTTGTTTTCCTTGTCTACTTAT	292	0.10427342491777754	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	289	0.10320212260697846	No Hit
TGGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCC	288	0.1028450218367121	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGT	25	3.8832233E-5	45.000004	9
AGGTCAT	25	3.8832233E-5	45.000004	44
TCACGCT	30	2.1596006E-6	45.000004	33
AGCACAC	25	3.8832233E-5	45.000004	31
ATTTACG	30	2.1596006E-6	45.000004	1
TATCAGT	30	2.1596006E-6	45.000004	22
TAATAGC	25	3.8832233E-5	45.000004	12
ATCTCGG	25	3.8832233E-5	45.000004	42
CTCACGC	30	2.1596006E-6	45.000004	32
TTAATTG	25	3.8832233E-5	45.000004	14
TTGGAGT	30	2.1596006E-6	45.000004	45
TTAATAG	25	3.8832233E-5	45.000004	17
CAAGTGC	30	2.1596006E-6	45.000004	15
GATCGAA	25	3.8832233E-5	45.000004	29
CGAGTCC	25	3.8832233E-5	45.000004	4
GCGAATT	25	3.8832233E-5	45.000004	9
CGACGAG	50	2.1827873E-11	45.000004	1
TTTCGTT	25	3.8832233E-5	45.000004	11
CTATATT	25	3.8832233E-5	45.000004	18
TTATGTC	25	3.8832233E-5	45.000004	18

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content