##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548557_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	280033
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.503483518013947	31.0	31.0	34.0	30.0	34.0
2	31.705820385454572	31.0	31.0	34.0	30.0	34.0
3	31.882242449996966	31.0	31.0	34.0	30.0	34.0
4	35.60446090282217	37.0	35.0	37.0	33.0	37.0
5	35.33827441765792	37.0	35.0	37.0	33.0	37.0
6	32.570850578324695	37.0	35.0	37.0	26.0	37.0
7	33.94245678187928	37.0	35.0	37.0	26.0	37.0
8	34.95659797238183	37.0	35.0	37.0	32.0	37.0
9	36.76822731606632	39.0	37.0	39.0	32.0	39.0
10	36.498859063039	38.0	35.0	39.0	32.0	39.0
11	36.557323601147004	38.0	35.0	39.0	32.0	39.0
12	36.45378223280828	38.0	35.0	39.0	32.0	39.0
13	36.490006534944094	38.0	35.0	39.0	32.0	39.0
14	37.53073030678527	39.0	36.0	41.0	32.0	41.0
15	37.66013291290669	39.0	36.0	41.0	32.0	41.0
16	37.51678909271407	39.0	36.0	41.0	32.0	41.0
17	37.43621287491117	39.0	36.0	40.0	32.0	41.0
18	37.02201883349463	38.0	36.0	40.0	32.0	41.0
19	36.67239575335764	38.0	35.0	40.0	31.0	41.0
20	36.60633211085836	38.0	35.0	40.0	31.0	41.0
21	36.5404148796749	38.0	35.0	40.0	31.0	41.0
22	36.42696753596898	38.0	35.0	40.0	31.0	41.0
23	36.17488653123025	38.0	35.0	40.0	30.0	41.0
24	36.04874782614906	38.0	35.0	40.0	30.0	41.0
25	36.085822028118116	38.0	35.0	40.0	30.0	41.0
26	35.9306938825067	38.0	35.0	40.0	30.0	41.0
27	35.89338399402928	38.0	35.0	40.0	30.0	41.0
28	35.79898797641707	38.0	34.0	40.0	30.0	41.0
29	35.590180443019214	38.0	34.0	40.0	29.0	41.0
30	35.56193734309885	38.0	34.0	40.0	28.0	41.0
31	35.09200344245143	37.0	34.0	40.0	25.0	41.0
32	35.1451936021826	37.0	34.0	40.0	26.0	41.0
33	34.99143672352901	37.0	34.0	40.0	25.0	41.0
34	35.02372220416879	37.0	34.0	40.0	25.0	41.0
35	34.97097127838505	37.0	34.0	40.0	25.0	41.0
36	34.74964022097396	37.0	34.0	40.0	25.0	41.0
37	34.709119996571836	37.0	34.0	40.0	24.0	41.0
38	34.46120278681441	37.0	33.0	40.0	24.0	41.0
39	34.300571718333195	37.0	33.0	40.0	23.0	41.0
40	34.09005367224577	36.0	33.0	40.0	22.0	41.0
41	34.01361268136255	36.0	33.0	40.0	22.0	41.0
42	33.87767870215296	36.0	33.0	40.0	21.0	41.0
43	33.80636924933847	36.0	33.0	40.0	21.0	41.0
44	33.67830934211325	36.0	33.0	40.0	21.0	41.0
45	33.45257523220478	36.0	33.0	40.0	20.0	41.0
46	33.17320101559459	35.0	32.0	39.0	20.0	41.0
47	33.13852653080173	35.0	32.0	39.0	20.0	41.0
48	33.17555073866294	35.0	33.0	39.0	20.0	41.0
49	33.05420432591873	35.0	32.0	39.0	20.0	41.0
50	32.84426121207144	35.0	32.0	39.0	18.0	41.0
51	31.742951723546867	35.0	30.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	1.0
11	1.0
12	4.0
13	10.0
14	17.0
15	50.0
16	73.0
17	165.0
18	359.0
19	690.0
20	1224.0
21	1696.0
22	2342.0
23	2812.0
24	3366.0
25	3535.0
26	3659.0
27	4164.0
28	4928.0
29	5995.0
30	7592.0
31	9658.0
32	12446.0
33	16280.0
34	22851.0
35	35664.0
36	28700.0
37	34368.0
38	42039.0
39	35331.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.374977234825895	39.90351137187403	16.450561183860472	25.2709502094396
2	19.294868819032043	43.7266322183457	15.517813971924738	21.460684990697526
3	16.548763895683724	40.328461288491	21.65459070895216	21.468184106873117
4	19.82694896672892	40.68056264797363	15.532098002735392	23.960390382562053
5	14.813611252959472	47.83900468873311	14.439726746490592	22.90765731181682
6	14.96109387107948	49.09421389621937	18.28820174765117	17.656490485049975
7	66.40145982794886	23.323679709177135	5.6479057825327725	4.626954680341246
8	67.37670203154629	19.213806944181577	4.600886324111801	8.808604700160338
9	64.50132662936154	21.302846450239795	8.164394910599821	6.031432009798845
10	32.280481228998006	38.32298336267511	12.731713762306585	16.66482164602029
11	21.80635853631536	37.72805347941136	20.798620162623692	19.666967821649592
12	20.48365728324876	32.542593194373524	28.886238407616244	18.087511114761476
13	21.6860155767356	35.573664532394396	23.14941453328715	19.590905357582855
14	15.096077962240164	36.46498805497924	28.53306574582281	19.905868236957787
15	16.763381458613807	38.30762802955366	23.49973038891845	21.429260122914084
16	16.860512868126257	37.76697746337039	26.33011109404963	19.042398574453724
17	17.685772748211818	35.11121903489946	25.35915409969539	21.84385411719333
18	18.68672620726843	36.83030214296173	23.02942867447765	21.453542975292198
19	18.001806929897548	39.54033988851314	25.5873414918956	16.870511689693714
20	19.87122946224195	40.012784207575535	22.134534144190148	17.981452185992367
21	18.06144275853203	38.07229862194813	22.811954305385438	21.054304314134406
22	18.252491670624533	38.057657490367205	21.59281227569608	22.097038563312182
23	18.15178925340942	39.001117725410936	20.391882385290305	22.45521063588934
24	15.722789814057627	41.972553234797324	21.369624294279603	20.93503265686544
25	18.546742705324014	37.005281520392245	24.255712719572337	20.19226305471141
26	20.18012162852235	38.185499566122566	20.896108672906408	20.738270132448676
27	15.545310731235249	36.712458888773824	25.001696228658766	22.740534151332163
28	18.43354176114958	36.031110619105604	25.935871843675567	19.59947577606925
29	19.68482286016291	38.31869815343192	21.67601675516814	20.320462231237034
30	19.372359686179845	36.362857234683055	22.855163498587665	21.409619580549435
31	19.163098634803756	39.15181425046334	18.732078005092255	22.95300910964065
32	18.767788082118894	37.10384133298575	21.061089228769468	23.067281356125886
33	18.62209096785022	37.8316127027886	23.14727192866555	20.39902440069563
34	19.773740951959233	34.3230976349216	21.543532369399323	24.359629043719845
35	18.41497252109573	34.677698699796096	24.73601325558059	22.171315523527586
36	19.599118675298982	36.11252959472633	23.96503269257552	20.323319037399163
37	19.31415226062643	36.02896801448401	22.29558659158028	22.36129313330929
38	21.594954880317676	37.872322190598965	21.64173508122257	18.89098784786079
39	21.619594833466056	33.496766452525236	22.450925426646144	22.43271328736256
40	23.13441630093596	35.00944531537355	21.145007909782063	20.711130473908433
41	20.939317866108638	31.944806504947632	24.491042127177867	22.624833501765863
42	22.36200733484982	32.55901982980578	23.411883599432922	21.667089235911483
43	19.27558537743766	32.81291847746516	24.07751943520942	23.833976709887764
44	20.080133412847772	30.75994614920384	24.446047430124306	24.713873007824077
45	21.970981991408156	34.149189559801876	24.67066381462185	19.209164634168115
46	18.696367928065623	31.72019012045009	28.445218956337285	21.138222995147
47	20.029782204240217	35.52724143225977	23.543296682890947	20.899679680609072
48	22.349865908660764	30.40105987508615	26.874332667935562	20.37474154831752
49	21.11072623583649	29.993250795441966	25.442358579167458	23.45366438955409
50	19.286655501315916	30.01539104319848	26.113350926497947	24.584602528987656
51	20.77540861255638	29.641863637499867	28.201676231015632	21.381051518928125
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21451.0
1	16006.5
2	10562.0
3	6457.0
4	2352.0
5	1894.0
6	1436.0
7	1459.5
8	1483.0
9	1520.5
10	1558.0
11	1648.0
12	1738.0
13	1721.5
14	1705.0
15	1847.5
16	1990.0
17	1914.5
18	1839.0
19	1904.0
20	1969.0
21	1810.0
22	1651.0
23	1587.5
24	1524.0
25	1545.5
26	1753.0
27	1939.0
28	2473.0
29	3007.0
30	2546.0
31	2085.0
32	2762.5
33	3440.0
34	4360.0
35	5280.0
36	5242.5
37	5205.0
38	5551.0
39	5897.0
40	6720.0
41	7543.0
42	8661.0
43	9779.0
44	15955.0
45	22131.0
46	22017.5
47	21904.0
48	22465.0
49	23026.0
50	21808.0
51	20590.0
52	18404.5
53	16219.0
54	15816.0
55	15413.0
56	14177.5
57	12942.0
58	11622.5
59	10303.0
60	9823.0
61	9343.0
62	9038.0
63	8733.0
64	7434.0
65	6135.0
66	5360.0
67	4585.0
68	4406.0
69	4227.0
70	3285.0
71	2343.0
72	2109.5
73	1876.0
74	1611.0
75	1053.0
76	760.0
77	646.5
78	533.0
79	447.0
80	361.0
81	265.5
82	170.0
83	107.0
84	44.0
85	29.0
86	14.0
87	13.5
88	13.0
89	10.5
90	8.0
91	7.5
92	7.0
93	6.5
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	280033.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.60662850449768
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.30648624357117	23.259044469758923
2	8.015429170619663	4.585888091760614
3	2.867379038298297	2.460781407905497
4	1.5204473960153793	1.739794952737713
5	0.9225046187646677	1.3194873461342056
6	0.6366405352773756	1.092728357015066
7	0.488091077045988	0.9773848082190314
8	0.3545213961152444	0.8113329500451733
9	0.2995955460128826	0.7713376637753407
>10	2.4691666250561743	15.53531190966779
>50	0.6865731262795226	14.228680191263173
>100	0.4169371348679283	18.27820292608371
>500	0.006241573875268388	1.0880860470016034
>1k	0.008738203425375742	7.105591126760061
>5k	0.0	0.0
>10k+	0.0012483147750536774	6.746347751872101
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18892	6.746347751872101	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4169	1.4887531112404608	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3548	1.2669935329050503	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGT	3325	1.1873600611356518	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCC	2830	1.0105951798538029	No Hit
AATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCT	2404	0.858470251720333	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1866	0.6663500373170305	No Hit
CTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT	1756	0.6270689525877308	Illumina Single End Adapter 2 (95% over 22bp)
AATGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTT	767	0.2738962907942992	No Hit
AAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCT	676	0.24140012070006037	No Hit
AATGATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCT	561	0.20033353211942878	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTT	540	0.19283441594383519	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTAT	503	0.17962168744397983	No Hit
AACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTG	494	0.17640778051158254	No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	473	0.16890866433598897	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTACAGGTC	461	0.16462345509279264	No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	386	0.13784089732281551	No Hit
TGGTAAGGGGCATGTTTAGACCTGGCTTTGAGCCAACCCAACCTAAGAACT	384	0.13712669578228281	No Hit
CAACCAGGGATTGATTTTAAAGATTTTTTTTTAAATTTCACATTTTTTTTT	348	0.12427106805269379	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCT	310	0.11070123878257207	No Hit
ATGATGGGGTCCATAAGGTGAGCCACGAGGGCTGTTGTTTTTCTTAGTTTT	307	0.10962993647177298	No Hit
GCAGCAGGGTGATTTTAGTGCAATAATCTCTGATTATTTAGAAGGAAAACG	300	0.10713023107990845	No Hit
ATTTACGGGATTATGTTTTATAGCTAATGATGTGTAAAGTCTAAAGATTTT	299	0.10677313030964207	No Hit
ACTCAAGGGCGTTATTGTTTAGCTAAGACTTTGTTTTCCTTGTCTACTTAT	292	0.10427342491777754	No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	289	0.10320212260697846	No Hit
TGGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCC	288	0.1028450218367121	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5710077026636144E-4	0.0	0.0	0.728128470573111	0.0
2	3.5710077026636144E-4	0.0	0.0	0.9159634757332171	0.0
3	3.5710077026636144E-4	0.0	0.0	1.2987755014587568	0.0
4	3.5710077026636144E-4	0.0	0.0	3.2003371031271315	0.0
5	3.5710077026636144E-4	0.0	0.0	3.45566415386758	0.0
6	3.5710077026636144E-4	0.0	0.0	4.7005174390161155	0.0
7	3.5710077026636144E-4	0.0	0.0	5.740037781261494	0.0
8	3.5710077026636144E-4	0.0	0.0	6.459952934118479	0.0
9	3.5710077026636144E-4	0.0	0.0	8.64148153967568	0.0
10	3.5710077026636144E-4	0.0	0.0	10.22272375041513	0.0
11	3.5710077026636144E-4	0.0	0.0	12.502812168565848	0.0
12	3.5710077026636144E-4	0.0	0.0	13.193445058260991	0.0
13	3.5710077026636144E-4	0.0	0.0	13.507693736095389	0.0
14	3.5710077026636144E-4	0.0	0.0	13.960497512793134	0.0
15	3.5710077026636144E-4	0.0	0.0	14.245463927465691	0.0
16	3.5710077026636144E-4	0.0	0.0	14.750404416622327	0.0
17	3.5710077026636144E-4	0.0	0.0	15.334264176007828	0.0
18	3.5710077026636144E-4	0.0	0.0	16.59233018965622	0.0
19	3.5710077026636144E-4	0.0	0.0	16.921577099841805	0.0
20	3.5710077026636144E-4	0.0	0.0	17.306174629418674	0.0
21	3.5710077026636144E-4	0.0	0.0	17.590069741780432	0.0
22	3.5710077026636144E-4	0.0	0.0	17.87717876107459	0.0
23	3.5710077026636144E-4	0.0	0.0	18.16535908267954	0.0
24	3.5710077026636144E-4	0.0	0.0	18.35497959169098	0.0
25	3.5710077026636144E-4	0.0	0.0	18.52067434909457	0.0
26	3.5710077026636144E-4	0.0	0.0	18.68958301343056	0.0
27	3.5710077026636144E-4	0.0	0.0	18.93098313413062	0.0
28	3.5710077026636144E-4	0.0	0.0	19.125603053925786	0.0
29	3.5710077026636144E-4	0.0	0.0	19.322722679112818	0.0
30	3.5710077026636144E-4	0.0	0.0	19.6180450161231	0.0
31	3.5710077026636144E-4	0.0	0.0	19.79338149432388	0.0
32	3.5710077026636144E-4	0.0	0.0	19.97514578638946	0.0
33	3.5710077026636144E-4	0.0	0.0	20.156552977684772	0.0
34	3.5710077026636144E-4	0.0	0.0	20.330103952034225	0.0
35	3.5710077026636144E-4	0.0	0.0	20.52865198030232	0.0
36	3.5710077026636144E-4	0.0	0.0	20.7104162723679	0.0
37	3.5710077026636144E-4	0.0	0.0	20.899679680609072	0.0
38	3.5710077026636144E-4	0.0	0.0	21.09108569347184	0.0
39	3.5710077026636144E-4	0.0	0.0	21.28963372173994	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGT	25	3.8832233E-5	45.000004	9
AGGTCAT	25	3.8832233E-5	45.000004	44
TCACGCT	30	2.1596006E-6	45.000004	33
AGCACAC	25	3.8832233E-5	45.000004	31
ATTTACG	30	2.1596006E-6	45.000004	1
TATCAGT	30	2.1596006E-6	45.000004	22
TAATAGC	25	3.8832233E-5	45.000004	12
ATCTCGG	25	3.8832233E-5	45.000004	42
CTCACGC	30	2.1596006E-6	45.000004	32
TTAATTG	25	3.8832233E-5	45.000004	14
TTGGAGT	30	2.1596006E-6	45.000004	45
TTAATAG	25	3.8832233E-5	45.000004	17
CAAGTGC	30	2.1596006E-6	45.000004	15
GATCGAA	25	3.8832233E-5	45.000004	29
CGAGTCC	25	3.8832233E-5	45.000004	4
GCGAATT	25	3.8832233E-5	45.000004	9
CGACGAG	50	2.1827873E-11	45.000004	1
TTTCGTT	25	3.8832233E-5	45.000004	11
CTATATT	25	3.8832233E-5	45.000004	18
TTATGTC	25	3.8832233E-5	45.000004	18
>>END_MODULE