FastQCFastQC Report
Sat 18 Jun 2016
SRR3548553_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548553_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535833
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA156502.9206861092915144No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38360.7158946910697923No Hit
CTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGCT32680.6098915147070075TruSeq Adapter, Index 19 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30460.5684606957764826No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18000.3359255588961486No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGT15040.28068446698878197No Hit
AAAAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTT14150.2640748143544724No Hit
AATCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT12430.2319752609488404No Hit
AAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT10280.19185081919180041No Hit
AACCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCAAATCCCCTC9600.17916029807794595No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCC9070.16926915662155934No Hit
AAAAAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCT7610.14202186128887173No Hit
AACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT6280.1172006949926563No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCC302.163324E-645.00000417
CGGTTAC302.163324E-645.00000435
ATAGCGG351.210301E-745.0000042
CACGCAT351.210301E-745.0000041
CGACATT302.163324E-645.00000412
ATATGCG302.163324E-645.0000041
CATTCCG302.163324E-645.00000444
ATCACGC351.210301E-745.00000442
ATCCGGA302.163324E-645.00000418
TGTTGCG302.163324E-645.00000430
GTTACGA302.163324E-645.00000437
TCATGCG302.163324E-645.0000041
GCCTATT302.163324E-645.00000419
CTCGATC351.210301E-745.00000416
TTACGAA302.163324E-645.00000438
CGAGACC351.210301E-745.00000421
TCGAGTA302.163324E-645.00000430
ACGTAGC302.163324E-645.00000413
TCGAGAC302.163324E-645.00000420
GACGAGC351.210301E-745.0000049