Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548553_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 535833 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 15650 | 2.9206861092915144 | No Hit |
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3836 | 0.7158946910697923 | No Hit |
CTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGCT | 3268 | 0.6098915147070075 | TruSeq Adapter, Index 19 (95% over 22bp) |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 3046 | 0.5684606957764826 | No Hit |
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1800 | 0.3359255588961486 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGT | 1504 | 0.28068446698878197 | No Hit |
AAAAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTT | 1415 | 0.2640748143544724 | No Hit |
AATCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT | 1243 | 0.2319752609488404 | No Hit |
AAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT | 1028 | 0.19185081919180041 | No Hit |
AACCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCAAATCCCCTC | 960 | 0.17916029807794595 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCC | 907 | 0.16926915662155934 | No Hit |
AAAAAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCT | 761 | 0.14202186128887173 | No Hit |
AACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT | 628 | 0.1172006949926563 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACTATCC | 30 | 2.163324E-6 | 45.000004 | 17 |
CGGTTAC | 30 | 2.163324E-6 | 45.000004 | 35 |
ATAGCGG | 35 | 1.210301E-7 | 45.000004 | 2 |
CACGCAT | 35 | 1.210301E-7 | 45.000004 | 1 |
CGACATT | 30 | 2.163324E-6 | 45.000004 | 12 |
ATATGCG | 30 | 2.163324E-6 | 45.000004 | 1 |
CATTCCG | 30 | 2.163324E-6 | 45.000004 | 44 |
ATCACGC | 35 | 1.210301E-7 | 45.000004 | 42 |
ATCCGGA | 30 | 2.163324E-6 | 45.000004 | 18 |
TGTTGCG | 30 | 2.163324E-6 | 45.000004 | 30 |
GTTACGA | 30 | 2.163324E-6 | 45.000004 | 37 |
TCATGCG | 30 | 2.163324E-6 | 45.000004 | 1 |
GCCTATT | 30 | 2.163324E-6 | 45.000004 | 19 |
CTCGATC | 35 | 1.210301E-7 | 45.000004 | 16 |
TTACGAA | 30 | 2.163324E-6 | 45.000004 | 38 |
CGAGACC | 35 | 1.210301E-7 | 45.000004 | 21 |
TCGAGTA | 30 | 2.163324E-6 | 45.000004 | 30 |
ACGTAGC | 30 | 2.163324E-6 | 45.000004 | 13 |
TCGAGAC | 30 | 2.163324E-6 | 45.000004 | 20 |
GACGAGC | 35 | 1.210301E-7 | 45.000004 | 9 |