##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548553_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	535833
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.451379440982546	31.0	31.0	34.0	30.0	34.0
2	31.659683147547838	31.0	31.0	34.0	30.0	34.0
3	31.852601463515686	31.0	31.0	34.0	30.0	34.0
4	35.60142805687593	37.0	35.0	37.0	33.0	37.0
5	35.333434111001004	37.0	35.0	37.0	33.0	37.0
6	32.604938478966396	37.0	35.0	37.0	26.0	37.0
7	34.07971140261984	37.0	35.0	37.0	27.0	37.0
8	35.117114847349825	37.0	35.0	37.0	32.0	37.0
9	36.881743005749925	39.0	37.0	39.0	33.0	39.0
10	36.52075740016012	38.0	35.0	39.0	32.0	39.0
11	36.58947283948544	38.0	35.0	39.0	32.0	39.0
12	36.495574180761544	38.0	35.0	39.0	32.0	39.0
13	36.4596842672997	38.0	35.0	39.0	32.0	39.0
14	37.481265618205676	39.0	36.0	40.0	32.0	41.0
15	37.55757484141514	39.0	36.0	41.0	32.0	41.0
16	37.488773554447	39.0	36.0	41.0	32.0	41.0
17	37.42746900620156	39.0	36.0	40.0	32.0	41.0
18	37.261769991769825	39.0	36.0	40.0	32.0	41.0
19	37.103655429956724	39.0	35.0	40.0	32.0	41.0
20	37.01273717744148	39.0	35.0	40.0	31.0	41.0
21	36.888239432808355	39.0	35.0	40.0	31.0	41.0
22	36.82846147960279	39.0	35.0	40.0	31.0	41.0
23	36.6321185891873	38.0	35.0	40.0	31.0	41.0
24	36.5571847945162	38.0	35.0	40.0	31.0	41.0
25	36.4800376236626	38.0	35.0	40.0	31.0	41.0
26	36.40010413692326	38.0	35.0	40.0	30.0	41.0
27	36.27957218013822	38.0	35.0	40.0	30.0	41.0
28	36.103560251048364	38.0	35.0	40.0	30.0	41.0
29	35.84859088559308	38.0	35.0	40.0	29.0	41.0
30	35.75166889683913	38.0	34.0	40.0	29.0	41.0
31	35.317733323628815	38.0	34.0	40.0	26.0	41.0
32	35.32292150726066	38.0	34.0	40.0	26.0	41.0
33	35.24172270091614	38.0	34.0	40.0	26.0	41.0
34	35.16173136033055	38.0	34.0	40.0	25.0	41.0
35	35.09595900215179	38.0	34.0	40.0	25.0	41.0
36	34.9508186319245	38.0	34.0	40.0	25.0	41.0
37	34.94887959494842	38.0	34.0	40.0	25.0	41.0
38	34.86215108065386	38.0	34.0	40.0	25.0	41.0
39	34.81447764508718	37.0	34.0	40.0	25.0	41.0
40	34.683313644363075	37.0	33.0	40.0	24.0	41.0
41	34.5942187211314	37.0	33.0	40.0	24.0	41.0
42	34.38364565079045	37.0	33.0	40.0	23.0	41.0
43	34.29098431787516	37.0	33.0	40.0	23.0	41.0
44	34.07103145942859	37.0	33.0	40.0	22.0	41.0
45	33.89659278170624	36.0	33.0	40.0	22.0	41.0
46	33.58112695559997	36.0	33.0	40.0	21.0	41.0
47	33.58141435857814	36.0	33.0	40.0	22.0	41.0
48	33.57015711984891	36.0	33.0	40.0	22.0	41.0
49	33.45646684694672	36.0	32.0	40.0	20.0	41.0
50	33.32872928692335	36.0	32.0	39.0	20.0	41.0
51	32.172426856875184	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	3.0
10	1.0
11	5.0
12	10.0
13	10.0
14	33.0
15	58.0
16	89.0
17	210.0
18	476.0
19	945.0
20	1717.0
21	2544.0
22	3580.0
23	4587.0
24	5564.0
25	6031.0
26	6574.0
27	7627.0
28	9010.0
29	11090.0
30	14255.0
31	18081.0
32	23261.0
33	31011.0
34	43613.0
35	58489.0
36	57470.0
37	71796.0
38	84293.0
39	73385.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.940850974090807	35.032370160105856	19.331956038541858	27.69482282726148
2	20.569841723074166	36.544408425759514	18.187756259879478	24.69799359128684
3	19.388130257001716	34.76157683457346	18.85587487146182	26.99441803696301
4	19.185641795111536	36.9734600145941	17.029559582929757	26.81133860736461
5	15.940974146795735	39.51081773612301	15.93444226092831	28.613765856152945
6	17.2523902036642	45.937633553737825	16.797211071359918	20.012765171238055
7	76.00800995832657	12.77188974923157	6.334436288918376	4.885664003523486
8	77.3660823428195	11.73481289879496	5.2010607782648695	5.698043980120672
9	70.6766847133342	14.876463375715943	9.293753837482948	5.15309807346692
10	32.16076650747527	35.865092295547306	13.846105036457255	18.12803616052016
11	24.556904856550453	34.014142466029526	22.668629964933103	18.760322712486914
12	23.141351876424185	28.540235483816783	28.89389044721023	19.4245221925488
13	24.215007287718375	29.301853375958554	25.558336272681974	20.924803063641097
14	17.581970502003422	33.02110918887041	28.227824714043372	21.169095595082794
15	16.73431834172214	33.543286807643426	28.462039478718182	21.26035537191625
16	18.68548596297727	33.07616365546728	26.542038284316195	21.69631209723925
17	17.17904645663854	32.908574126640204	26.686486274641542	23.225893142079716
18	19.831551994744633	31.27280328012646	24.806982772617587	24.088661952511323
19	19.52735273863312	35.316600507994096	25.248351631945027	19.907695121427757
20	21.116840508143394	32.16431238837474	24.125800389300398	22.59304671418147
21	18.97139593865999	34.9231943534646	24.988382574421507	21.117027133453895
22	18.551488990039807	32.416069932236354	24.80772927385958	24.224711803864263
23	19.043433308512167	32.79809194282547	23.969968255034686	24.188506493627678
24	17.02769332982478	33.91000554277172	24.51715366541441	24.54514746198909
25	18.72579703004481	33.55168494661583	24.230870439110692	23.491647584228666
26	19.97469360789649	33.32325556656645	24.22321880138028	22.47883202415678
27	16.88455171667292	33.13942963572606	24.246733590503013	25.72928505709801
28	19.387010505138726	34.08916584084967	25.750560342494772	20.773263311516835
29	20.062034253209486	35.349633187952215	22.38738562201283	22.200946936825467
30	19.469872142999776	34.40288298779657	22.3375566641099	23.78968820509375
31	19.822780605151234	35.21283683535729	21.867820757586788	23.0965618019047
32	20.719141971472453	32.464592512965794	22.290900336485432	24.525365179076317
33	19.457181621885926	32.48586779836255	23.671741008859104	24.385209570892425
34	17.870306606722615	31.438899806469557	24.713670117368657	25.977123469439174
35	18.44492593774553	32.56630330718713	23.19136745963761	25.79740329542973
36	18.183650503048522	34.59660006009335	24.40387210194221	22.815877334915914
37	18.442126558088063	32.43734521763311	23.009594407212695	26.11093381706614
38	20.03329395539282	30.223969035128484	25.683001980094545	24.059735029384154
39	19.60256273876376	27.13737302480437	24.544587586057595	28.715476650374278
40	21.40610973941508	27.743718658611915	23.81954825477341	27.030623347199594
41	17.550430824529283	28.398773498459406	26.638710195154086	27.41208548185722
42	21.015502964543057	30.14633290596137	23.978179768696588	24.859984360798983
43	18.661597923233543	29.76449752068275	26.090964908842867	25.482939647240837
44	21.025953981930936	27.935942728424713	25.081881854980935	25.956221434663412
45	21.417680508665946	27.75249004820532	27.108072851056207	23.72175659207253
46	17.880757624110498	29.05438821423839	28.303967840726497	24.760886320924616
47	19.277461447876483	28.924123747510883	26.12642371783746	25.67199108677517
48	19.606108619663214	27.089970195937912	29.014637023102345	24.289284161296525
49	19.9653623423716	26.635164314254627	27.316906573503314	26.082566769870464
50	19.48797479811807	26.053826472053792	27.06776178398867	27.39043694583947
51	19.40324690715204	25.687107736925498	28.840328983097347	26.069316372825114
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18255.0
1	14156.5
2	10058.0
3	6338.0
4	2618.0
5	2283.0
6	1948.0
7	2086.0
8	2224.0
9	2318.5
10	2413.0
11	2670.0
12	2927.0
13	2884.5
14	2842.0
15	2887.0
16	2932.0
17	2896.5
18	2861.0
19	3019.0
20	3177.0
21	2825.5
22	2474.0
23	2649.5
24	2825.0
25	3107.0
26	3952.5
27	4516.0
28	5305.0
29	6094.0
30	6521.5
31	6949.0
32	8122.5
33	9296.0
34	10976.5
35	12657.0
36	13736.5
37	14816.0
38	15568.0
39	16320.0
40	16439.5
41	16559.0
42	19426.0
43	22293.0
44	27327.0
45	32361.0
46	37197.5
47	42034.0
48	46619.5
49	51205.0
50	48083.5
51	44962.0
52	41864.5
53	38767.0
54	33702.0
55	28637.0
56	26544.0
57	24451.0
58	23213.0
59	21975.0
60	20099.5
61	18224.0
62	17322.5
63	16421.0
64	14223.0
65	12025.0
66	10667.0
67	9309.0
68	8154.5
69	7000.0
70	6203.5
71	5407.0
72	5110.0
73	4813.0
74	3937.5
75	2489.5
76	1917.0
77	1605.5
78	1294.0
79	1000.0
80	706.0
81	493.5
82	281.0
83	280.0
84	279.0
85	215.5
86	152.0
87	115.5
88	79.0
89	51.0
90	23.0
91	12.5
92	2.0
93	3.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	535833.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.878950144556182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.24430291461104	23.378576258812153
2	8.99360684768155	5.374390612432322
3	3.5529810328177396	3.18478029432345
4	1.8794835291277905	2.2462797865729502
5	1.1738834365784696	1.7537202338524187
6	0.8034148941004827	1.4403116139733323
7	0.577201936991802	1.2072331529103517
8	0.42765978620456485	1.02224203446702
9	0.36607726440689287	0.984420389904216
>10	2.8340639048173903	18.496611940741108
>50	0.7203103490601088	15.312741253487067
>100	0.41755293439386343	18.612862014696784
>500	0.003784467683328067	0.8062313183973031
>1k	0.00504595691110409	3.230202149913458
>5k	0.0	0.0
>10k+	6.307446138880112E-4	2.9493969455160807
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15650	2.9206861092915144	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3836	0.7158946910697923	No Hit
CTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGCT	3268	0.6098915147070075	TruSeq Adapter, Index 19 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3046	0.5684606957764826	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1800	0.3359255588961486	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGT	1504	0.28068446698878197	No Hit
AAAAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTT	1415	0.2640748143544724	No Hit
AATCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT	1243	0.2319752609488404	No Hit
AAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT	1028	0.19185081919180041	No Hit
AACCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCAAATCCCCTC	960	0.17916029807794595	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCC	907	0.16926915662155934	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCT	761	0.14202186128887173	No Hit
AACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT	628	0.1172006949926563	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8662531049786033E-4	0.0	0.0	0.7593783884157937	0.0
2	1.8662531049786033E-4	0.0	0.0	1.0227067015282747	0.0
3	1.8662531049786033E-4	0.0	0.0	1.3487411189680367	0.0
4	1.8662531049786033E-4	0.0	0.0	2.9400951415832917	0.0
5	1.8662531049786033E-4	0.0	0.0	3.197451444759841	0.0
6	1.8662531049786033E-4	0.0	0.0	4.897608023395349	0.0
7	1.8662531049786033E-4	0.0	0.0	6.102461027969535	0.0
8	1.8662531049786033E-4	0.0	0.0	7.0171116747195486	0.0
9	1.8662531049786033E-4	0.0	0.0	8.458978823626017	0.0
10	1.8662531049786033E-4	0.0	0.0	10.490581953705725	0.0
11	1.8662531049786033E-4	0.0	0.0	12.147628085616228	0.0
12	1.8662531049786033E-4	0.0	0.0	13.09549803763486	0.0
13	1.8662531049786033E-4	0.0	0.0	13.472667790151036	0.0
14	1.8662531049786033E-4	0.0	0.0	13.862714689091565	0.0
15	1.8662531049786033E-4	0.0	0.0	14.153476922847231	0.0
16	1.8662531049786033E-4	0.0	0.0	14.861160100255116	0.0
17	1.8662531049786033E-4	0.0	0.0	15.594970821132703	0.0
18	1.8662531049786033E-4	0.0	0.0	16.98402300716828	0.0
19	1.8662531049786033E-4	0.0	0.0	17.334132089662265	0.0
20	1.8662531049786033E-4	0.0	0.0	17.86489447271818	0.0
21	1.8662531049786033E-4	0.0	0.0	18.26632551559908	0.0
22	1.8662531049786033E-4	0.0	0.0	18.687538841392747	0.0
23	1.8662531049786033E-4	0.0	0.0	19.129280951341183	0.0
24	1.8662531049786033E-4	0.0	0.0	19.433853458073692	0.0
25	1.8662531049786033E-4	0.0	0.0	19.716777428788447	0.0
26	1.8662531049786033E-4	0.0	0.0	19.95976358305666	0.0
27	1.8662531049786033E-4	0.0	0.0	20.30334077968322	0.0
28	1.8662531049786033E-4	0.0	0.0	20.603247653653284	0.0
29	1.8662531049786033E-4	0.0	0.0	20.88691812561003	0.0
30	1.8662531049786033E-4	0.0	0.0	21.35908016116962	0.0
31	1.8662531049786033E-4	0.0	0.0	21.632112990427988	0.0
32	1.8662531049786033E-4	0.0	0.0	21.900106936302915	0.0
33	1.8662531049786033E-4	0.0	0.0	22.210464827660857	0.0
34	1.8662531049786033E-4	0.0	0.0	22.45886311593351	0.0
35	1.8662531049786033E-4	0.0	0.0	22.741040585406274	0.0
36	1.8662531049786033E-4	0.0	0.0	23.003622397276764	0.0
37	1.8662531049786033E-4	0.0	0.0	23.270496591288705	0.0
38	3.7325062099572066E-4	0.0	0.0	23.558086194765906	0.0
39	3.7325062099572066E-4	0.0	0.0	23.841570041412158	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCC	30	2.163324E-6	45.000004	17
CGGTTAC	30	2.163324E-6	45.000004	35
ATAGCGG	35	1.210301E-7	45.000004	2
CACGCAT	35	1.210301E-7	45.000004	1
CGACATT	30	2.163324E-6	45.000004	12
ATATGCG	30	2.163324E-6	45.000004	1
CATTCCG	30	2.163324E-6	45.000004	44
ATCACGC	35	1.210301E-7	45.000004	42
ATCCGGA	30	2.163324E-6	45.000004	18
TGTTGCG	30	2.163324E-6	45.000004	30
GTTACGA	30	2.163324E-6	45.000004	37
TCATGCG	30	2.163324E-6	45.000004	1
GCCTATT	30	2.163324E-6	45.000004	19
CTCGATC	35	1.210301E-7	45.000004	16
TTACGAA	30	2.163324E-6	45.000004	38
CGAGACC	35	1.210301E-7	45.000004	21
TCGAGTA	30	2.163324E-6	45.000004	30
ACGTAGC	30	2.163324E-6	45.000004	13
TCGAGAC	30	2.163324E-6	45.000004	20
GACGAGC	35	1.210301E-7	45.000004	9
>>END_MODULE