##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548551_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	734471
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65126737474999	31.0	31.0	34.0	30.0	34.0
2	31.857845987111812	33.0	31.0	34.0	30.0	34.0
3	32.03260850326289	33.0	31.0	34.0	30.0	34.0
4	35.76655170864473	37.0	35.0	37.0	35.0	37.0
5	35.5228756479153	37.0	35.0	37.0	33.0	37.0
6	32.75590867440648	37.0	35.0	37.0	26.0	37.0
7	34.21982488076452	37.0	35.0	37.0	27.0	37.0
8	35.3086820310128	37.0	35.0	37.0	32.0	37.0
9	37.13796732614358	39.0	37.0	39.0	34.0	39.0
10	36.68487659825916	38.0	37.0	39.0	32.0	39.0
11	36.743833316768125	39.0	37.0	39.0	32.0	39.0
12	36.6516921702831	39.0	35.0	39.0	32.0	39.0
13	36.61635925720689	39.0	35.0	39.0	32.0	39.0
14	37.771005254121675	40.0	37.0	41.0	33.0	41.0
15	37.80659277221292	40.0	37.0	41.0	33.0	41.0
16	37.75147282874341	40.0	37.0	41.0	33.0	41.0
17	37.72665088206342	39.0	37.0	41.0	33.0	41.0
18	37.60471005662579	39.0	36.0	41.0	33.0	41.0
19	37.543860819555846	39.0	36.0	41.0	32.0	41.0
20	37.409417117898464	39.0	35.0	41.0	32.0	41.0
21	37.320304001111005	39.0	35.0	41.0	32.0	41.0
22	37.28445643190813	39.0	35.0	41.0	32.0	41.0
23	37.11132910625471	39.0	35.0	41.0	32.0	41.0
24	36.987708160022656	39.0	35.0	40.0	31.0	41.0
25	36.988380752950086	39.0	35.0	40.0	31.0	41.0
26	36.95516909449114	39.0	35.0	40.0	31.0	41.0
27	36.848221372933715	39.0	35.0	40.0	31.0	41.0
28	36.730431834612936	39.0	35.0	40.0	31.0	41.0
29	36.55221785475533	39.0	35.0	40.0	30.0	41.0
30	36.52907875191805	39.0	35.0	40.0	30.0	41.0
31	36.15083645235823	38.0	35.0	40.0	30.0	41.0
32	36.23114595402678	38.0	35.0	40.0	30.0	41.0
33	36.208118496169355	38.0	35.0	40.0	30.0	41.0
34	36.11874668979442	38.0	35.0	40.0	30.0	41.0
35	36.093041113944594	38.0	35.0	40.0	30.0	41.0
36	35.99487658464391	38.0	35.0	40.0	30.0	41.0
37	35.882248584355274	38.0	35.0	40.0	29.0	41.0
38	35.76755379041514	38.0	35.0	40.0	29.0	41.0
39	35.69930194657107	38.0	35.0	40.0	28.0	41.0
40	35.5191137567038	38.0	34.0	40.0	27.0	41.0
41	35.452542033654154	38.0	34.0	40.0	27.0	41.0
42	35.36791513892312	38.0	34.0	40.0	27.0	41.0
43	35.30039851811712	38.0	34.0	40.0	27.0	41.0
44	35.09991681087477	38.0	34.0	40.0	26.0	41.0
45	34.890605619554755	37.0	34.0	40.0	26.0	41.0
46	34.58485495002525	37.0	33.0	40.0	24.0	41.0
47	34.52576752519841	37.0	33.0	40.0	24.0	41.0
48	34.51953991376106	37.0	33.0	40.0	24.0	41.0
49	34.38732094255594	37.0	33.0	40.0	24.0	41.0
50	34.25763032168731	36.0	33.0	40.0	24.0	41.0
51	33.17517505796689	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	7.0
10	16.0
11	19.0
12	24.0
13	28.0
14	47.0
15	73.0
16	166.0
17	316.0
18	516.0
19	995.0
20	1662.0
21	2479.0
22	3402.0
23	4408.0
24	5532.0
25	6175.0
26	7364.0
27	8639.0
28	10250.0
29	12561.0
30	15974.0
31	20708.0
32	27009.0
33	36549.0
34	53219.0
35	70420.0
36	77119.0
37	101514.0
38	134600.0
39	132644.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.578251557924002	32.53566172115713	18.98631804387103	26.899768677047835
2	23.59929799815105	32.31060178005667	18.259672607904193	25.83042761388809
3	19.89622462969947	33.79057852522428	19.80718095064339	26.506015894432867
4	20.56949831919844	35.41977831663878	17.828069454069663	26.182653910093112
5	18.571870094258315	36.65522532543831	15.914174964021724	28.858729616281646
6	17.301704219771782	43.705741955774975	16.629111292345105	22.363442532108145
7	82.18840498807985	10.329066770505575	3.877212306544438	3.605315934870131
8	83.93197280763978	8.829756382484808	3.642213239188477	3.59605757068693
9	77.67236555289453	12.108034217824802	6.2421797456945205	3.9774204835861457
10	36.52696975101808	35.10390471509427	12.423499362125938	15.945626171761717
11	26.46285557904941	32.586855028993654	22.097264561841108	18.853024830115825
12	26.12220223807339	27.665081398720986	27.21291923030317	18.999797132902458
13	24.18924641000121	28.863358798373252	26.32656701217611	20.620827779449428
14	21.1670712662583	31.01252466060607	26.07890577027548	21.741498302860155
15	20.371532708575288	32.06756972024763	26.679201765624512	20.881695805552567
16	22.37923621218537	30.29500143640797	25.30215624578778	22.023606105618875
17	21.339576375377654	30.76064269385721	25.69754285737626	22.202238073388873
18	22.902742245779613	29.081202661507398	25.453830035494935	22.56222505721805
19	21.964788262572654	32.14776349236389	24.332342597597453	21.555105647466
20	23.96105496336819	31.46332530487929	24.29884910364058	20.276770628111933
21	22.489928125140406	31.436094821987524	24.89805587967394	21.175921173198127
22	23.367021979084264	28.965200804388463	24.379315180585756	23.288462035941514
23	21.686901184662158	31.97893449843493	23.810878850220092	22.523285466682825
24	20.71940212751763	32.06212362366928	25.794074919227576	21.424399329585512
25	21.80889374801728	31.955243978319093	23.707947624889204	22.527914648774424
26	23.671458777814237	30.03467801996267	23.045293823718023	23.248569378505074
27	21.326505743589603	30.39412039413401	24.49613395219144	23.783239910084948
28	21.31411587387385	32.599244898709415	24.964770562758776	21.121868664657963
29	22.799674868034273	30.76050654144275	23.529860266777042	22.909958323745936
30	21.94736075352192	30.414679408717294	26.202532162604104	21.435427675156678
31	23.440408130477582	31.653666380292755	21.796095421058148	23.109830068171515
32	23.399970863383306	30.960650590697252	23.141689733154884	22.49768881276456
33	21.407924887435993	30.364030710538604	23.614274763741523	24.61376963828388
34	20.424632150214237	31.943126413432253	25.33918970252059	22.29305173383292
35	21.245222752157673	30.42339316324266	24.745701327894498	23.585682756705165
36	22.044573577445536	31.998540446117	24.109869552371705	21.847016424065757
37	21.1763296304415	31.146362484019107	23.65566509773701	24.021642787802378
38	22.011760845560953	31.852721210231582	24.320497337539535	21.81502060666793
39	22.254112143297693	28.879969392937227	23.94607819777772	24.919840265987357
40	22.332808238854902	30.225699857448422	24.733447610593203	22.708044293103473
41	20.89762563804425	28.520118561522512	26.395187829063367	24.18706797136987
42	22.210271065841948	29.510082767052747	24.826167404839676	23.453478762265632
43	21.904745047796307	28.20070499720207	25.256000577286237	24.638549377715393
44	23.381181830187987	27.05103400951161	25.909532166688678	23.658251993611728
45	22.657259442510323	28.7493992274712	25.912527519806773	22.680813810211703
46	22.02660145873697	29.089916416032764	25.696725942889508	23.18675618234076
47	22.13348110408716	29.79545822775848	25.656969437867527	22.414091230286832
48	21.323918847714886	29.696747727275824	26.637675279214562	22.341658145794728
49	21.525969030771808	28.891406195751774	26.11730075115287	23.46532402232355
50	20.851606121957165	28.457216146042523	26.56728448093934	24.12389325106097
51	20.933978332704765	27.42463623478667	27.460852777032724	24.180532655475844
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16824.0
1	12987.0
2	9150.0
3	5746.5
4	2343.0
5	2135.5
6	1928.0
7	2058.5
8	2189.0
9	2343.0
10	2497.0
11	2636.5
12	2776.0
13	3201.5
14	3627.0
15	3266.5
16	2906.0
17	2874.0
18	2842.0
19	2682.0
20	2522.0
21	2879.0
22	3236.0
23	3943.5
24	4651.0
25	5040.5
26	5968.0
27	6506.0
28	8223.0
29	9940.0
30	11644.5
31	13349.0
32	15635.5
33	17922.0
34	19209.5
35	20497.0
36	21663.5
37	22830.0
38	25683.5
39	28537.0
40	26997.0
41	25457.0
42	28679.5
43	31902.0
44	35636.0
45	39370.0
46	44435.5
47	49501.0
48	52425.5
49	55350.0
50	53026.5
51	50703.0
52	49244.0
53	47785.0
54	43183.0
55	38581.0
56	36895.5
57	35210.0
58	34782.5
59	34355.0
60	32485.0
61	30615.0
62	28025.0
63	25435.0
64	23118.5
65	20802.0
66	19593.5
67	18385.0
68	15833.0
69	13281.0
70	11791.0
71	10301.0
72	9837.5
73	9374.0
74	7711.5
75	4810.0
76	3571.0
77	3222.0
78	2873.0
79	2333.5
80	1794.0
81	1177.0
82	560.0
83	430.5
84	301.0
85	266.5
86	232.0
87	157.5
88	83.0
89	64.0
90	45.0
91	39.5
92	34.0
93	22.5
94	11.0
95	8.5
96	6.0
97	4.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	734471.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.81761432028299
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.39109799682716	18.432713239116545
2	8.625091414815564	4.108582015905221
3	3.211550160131223	2.2947438925274564
4	1.8162143042885543	1.7303156729010352
5	1.1598650732531175	1.3812609489154768
6	0.8245864944818779	1.1783809859570118
7	0.6096817486854986	1.0164815323814835
8	0.4744866606018842	0.9040912225867755
9	0.39802689824852117	0.8532046036423617
>10	3.6837593594422566	19.583655436200452
>50	1.0773632041829377	18.478254886669372
>100	0.722487522203281	26.279182770294145
>500	0.002894581419083658	0.49624468773270564
>1k	0.0023156651352669267	1.27294552852135
>5k	0.0	0.0
>10k+	5.789162838167317E-4	1.9899425766486265
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14432	1.9649516454700051	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3376	0.45965055121304993	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2954	0.4021942323114187	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1519	0.2068155175629807	No Hit
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT	1383	0.18829878919657822	TruSeq Adapter, Index 13 (95% over 22bp)
AATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGT	921	0.1253963737165933	No Hit
AATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT	789	0.10742425500802619	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2231538072980417	0.0
2	0.0	0.0	0.0	0.31437592498546574	0.0
3	0.0	0.0	0.0	0.43391774488032886	0.0
4	0.0	0.0	0.0	0.9672267523156122	0.0
5	0.0	0.0	0.0	1.0588573272464128	0.0
6	0.0	0.0	0.0	1.6424065756170088	0.0
7	0.0	0.0	0.0	2.0606667928345708	0.0
8	0.0	0.0	0.0	2.498669110148665	0.0
9	0.0	0.0	0.0	3.119115662837607	0.0
10	0.0	0.0	0.0	4.039914441822754	0.0
11	0.0	0.0	0.0	4.756076141876262	0.0
12	0.0	0.0	0.0	5.1231430512572995	0.0
13	0.0	0.0	0.0	5.290610521041675	0.0
14	1.3615241445884182E-4	0.0	0.0	5.452768046662156	0.0
15	1.3615241445884182E-4	0.0	0.0	5.592324271482468	0.0
16	1.3615241445884182E-4	0.0	0.0	5.850333096881974	0.0
17	1.3615241445884182E-4	0.0	0.0	6.1698828136168755	0.0
18	1.3615241445884182E-4	0.0	0.0	6.6299418220733015	0.0
19	1.3615241445884182E-4	0.0	0.0	6.794550091154042	0.0
20	1.3615241445884182E-4	0.0	0.0	6.997008731454339	0.0
21	1.3615241445884182E-4	0.0	0.0	7.199875828998014	0.0
22	1.3615241445884182E-4	0.0	0.0	7.402062164469394	0.0
23	1.3615241445884182E-4	0.0	0.0	7.637469689068731	0.0
24	1.3615241445884182E-4	0.0	0.0	7.803711787122977	0.0
25	1.3615241445884182E-4	0.0	0.0	7.953207138198786	0.0
26	1.3615241445884182E-4	0.0	0.0	8.087317266440744	0.0
27	1.3615241445884182E-4	0.0	0.0	8.241305647193695	0.0
28	1.3615241445884182E-4	0.0	0.0	8.395566332775562	0.0
29	1.3615241445884182E-4	0.0	0.0	8.558540772882797	0.0
30	1.3615241445884182E-4	0.0	0.0	8.779788446378413	0.0
31	1.3615241445884182E-4	0.0	0.0	8.921931567073445	0.0
32	1.3615241445884182E-4	0.0	0.0	9.083680635450548	0.0
33	1.3615241445884182E-4	0.0	0.0	9.243115112781853	0.0
34	1.3615241445884182E-4	0.0	0.0	9.40214113286978	0.0
35	1.3615241445884182E-4	0.0	0.0	9.576552375791556	0.0
36	1.3615241445884182E-4	0.0	0.0	9.733808414491518	0.0
37	1.3615241445884182E-4	0.0	0.0	9.882078393837197	0.0
38	1.3615241445884182E-4	0.0	0.0	10.03565831734677	0.0
39	1.3615241445884182E-4	0.0	0.0	10.223004039642136	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGG	85	0.0	45.000004	2
TGATCGC	20	7.031623E-4	45.0	19
CTATGCG	25	3.8894254E-5	45.0	1
AAGTACG	20	7.031623E-4	45.0	1
ACCGGAT	20	7.031623E-4	45.0	27
CGAACAT	25	3.8894254E-5	45.0	22
CGAGTTT	20	7.031623E-4	45.0	39
TTTACGG	55	1.8189894E-12	45.0	2
ACGTTGC	20	7.031623E-4	45.0	31
ACGTTAT	20	7.031623E-4	45.0	29
ACGCAAA	20	7.031623E-4	45.0	10
ATAATGC	35	1.2111195E-7	45.0	25
TAGCGTC	35	1.2111195E-7	45.0	31
CGGTTGA	20	7.031623E-4	45.0	31
GACGTAC	20	7.031623E-4	45.0	36
ATTCGTT	20	7.031623E-4	45.0	34
TATATCG	50	2.1827873E-11	45.0	1
CACGATT	20	7.031623E-4	45.0	36
CAATTCG	20	7.031623E-4	45.0	24
TCGATCC	25	3.8894254E-5	45.0	19
>>END_MODULE