FastQCFastQC Report
Sat 18 Jun 2016
SRR3548548_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548548_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804944
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA167272.0780327575582898No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38010.472206762209545No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29460.3659881929674611No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGT21490.266975093919577No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCC20150.2503279731260808No Hit
AATCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCT18370.22821463356457097No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16790.20858593889761276No Hit
GCAGTCTGGGGTGTGATTGCTCTTAGGTGAGTTCCGAGTCTTAATCTATGC16070.19964121727722672No Hit
CTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGCT12150.150942177344014No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA8330.10348545985807708No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCACG502.1827873E-1145.0000041
CACGGTT502.1827873E-1145.00000444
CAACGTT253.8897597E-545.00000438
TTTACGT253.8897597E-545.00000412
CATGCGC253.8897597E-545.00000435
CTATCAT253.8897597E-545.00000436
CACGCGA253.8897597E-545.00000411
CGTCTAC253.8897597E-545.0000045
TCGATAG502.1827873E-1145.0000041
GTTACGC253.8897597E-545.0000049
CGCTACG502.1827873E-1145.0000041
GTCCGTT253.8897597E-545.00000411
CACGTTA253.8897597E-545.00000433
CGTCATA253.8897597E-545.00000428
TAATGCG253.8897597E-545.0000041
CGATACG253.8897597E-545.0000041
GCCCGGT253.8897597E-545.00000426
TACGTTA253.8897597E-545.00000414
AAGTCGA502.1827873E-1145.00000432
TCACGCG406.8102963E-945.01