##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548548_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	804944
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.698432437536027	31.0	31.0	34.0	30.0	34.0
2	31.882169442843228	33.0	31.0	34.0	30.0	34.0
3	32.05540136953626	33.0	31.0	34.0	30.0	34.0
4	35.77107848496293	37.0	35.0	37.0	35.0	37.0
5	35.5141351448051	37.0	35.0	37.0	33.0	37.0
6	34.24325418911129	37.0	35.0	37.0	32.0	37.0
7	34.9494486572979	37.0	35.0	37.0	32.0	37.0
8	35.60237606591266	37.0	35.0	37.0	33.0	37.0
9	37.25395803931702	39.0	37.0	39.0	34.0	39.0
10	36.73194408555129	39.0	37.0	39.0	32.0	39.0
11	36.74545309984297	39.0	37.0	39.0	32.0	39.0
12	36.6190480331551	39.0	35.0	39.0	32.0	39.0
13	36.60137723866505	39.0	35.0	39.0	32.0	39.0
14	37.767339094396625	40.0	37.0	41.0	33.0	41.0
15	37.792097338448386	40.0	37.0	41.0	33.0	41.0
16	37.71147185394263	40.0	37.0	41.0	33.0	41.0
17	37.65022287264704	39.0	36.0	41.0	33.0	41.0
18	37.54776978274265	39.0	36.0	41.0	33.0	41.0
19	37.48904023137013	39.0	36.0	41.0	32.0	41.0
20	37.391455803136616	39.0	36.0	41.0	32.0	41.0
21	37.37933570534099	39.0	35.0	41.0	32.0	41.0
22	37.30357764018366	39.0	35.0	41.0	32.0	41.0
23	37.15509153431792	39.0	35.0	41.0	32.0	41.0
24	36.954184390466914	39.0	35.0	40.0	31.0	41.0
25	36.967966715696996	39.0	35.0	40.0	31.0	41.0
26	36.89802023494802	39.0	35.0	40.0	31.0	41.0
27	36.819962630940786	39.0	35.0	40.0	31.0	41.0
28	36.664248693076786	39.0	35.0	40.0	31.0	41.0
29	36.494328798028185	39.0	35.0	40.0	30.0	41.0
30	36.513754994136235	39.0	35.0	40.0	30.0	41.0
31	36.42953050150073	39.0	35.0	40.0	30.0	41.0
32	36.33791294798147	38.0	35.0	40.0	30.0	41.0
33	36.27287612554414	38.0	35.0	40.0	30.0	41.0
34	36.16945402413087	38.0	35.0	40.0	30.0	41.0
35	36.02174188514977	38.0	35.0	40.0	29.0	41.0
36	35.97023271184082	38.0	35.0	40.0	29.0	41.0
37	35.881338329126	38.0	35.0	40.0	29.0	41.0
38	35.78359239897434	38.0	35.0	40.0	29.0	41.0
39	35.69545210598501	38.0	35.0	40.0	28.0	41.0
40	35.52487129539446	38.0	34.0	40.0	27.0	41.0
41	35.4080159613588	38.0	34.0	40.0	27.0	41.0
42	35.29470000397543	38.0	34.0	40.0	27.0	41.0
43	35.2300942674273	38.0	34.0	40.0	27.0	41.0
44	35.07814208193365	38.0	34.0	40.0	26.0	41.0
45	34.86781440696495	37.0	34.0	40.0	26.0	41.0
46	34.72784566379773	37.0	33.0	40.0	25.0	41.0
47	34.607781410880754	37.0	33.0	40.0	25.0	41.0
48	34.45967297103898	37.0	33.0	40.0	24.0	41.0
49	34.29535719255004	36.0	33.0	40.0	24.0	41.0
50	34.20684420282653	36.0	33.0	40.0	24.0	41.0
51	32.98491199387784	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	10.0
11	14.0
12	15.0
13	33.0
14	50.0
15	92.0
16	160.0
17	296.0
18	553.0
19	966.0
20	1646.0
21	2451.0
22	3488.0
23	4548.0
24	5603.0
25	6792.0
26	7905.0
27	9361.0
28	11418.0
29	14313.0
30	18211.0
31	22925.0
32	30054.0
33	39732.0
34	57450.0
35	75794.0
36	81708.0
37	108615.0
38	149268.0
39	151448.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.474855393667138	32.16149197956628	19.003309546999542	26.36034307976704
2	22.515603569937785	32.34833727563657	19.17574390268143	25.96031525174422
3	20.786663420064006	32.348088811147115	20.797595857600033	26.067651911188854
4	21.54050468107098	32.5975471585601	17.851428173885388	28.010519986483533
5	17.266542765707925	36.02436939712576	16.7484943051939	29.960593531972414
6	18.188842950565505	40.79426643344134	18.97051223439146	22.046378381601702
7	81.85041443876841	9.852238168121012	5.087061957105091	3.210285436005486
8	83.67389532687987	7.9159544017968955	3.8815122542686202	4.528638017054602
9	77.51346677532847	11.382779423164841	6.389637043073804	4.714116758432885
10	35.67192251883361	33.65203045180783	14.048927627263511	16.627119402095055
11	27.548997197320556	32.06757240255222	21.925500407481763	18.45792999264545
12	26.08964101850563	25.91670973384484	28.574907074280944	19.41874217336858
13	24.2654147369258	27.730873203601742	26.543585640740226	21.460126418732234
14	20.759580790713393	29.180042338349004	27.530238128366697	22.530138742570912
15	20.940835635770934	30.52063249120436	27.740439086445768	20.79809278657894
16	22.044017968951877	28.450177900574452	27.065982229819713	22.439821900653957
17	21.83307161740444	28.81219066171063	26.623839670834244	22.730898050050687
18	22.978120217058578	28.757280009541038	26.18641793714842	22.07818183625196
19	22.086753861138167	30.62337255759407	25.791235166669978	21.498638414597785
20	22.69064680275895	30.342234987775544	24.923721401737264	22.04339680772824
21	23.983159076904727	28.800264366216783	26.01435627820072	21.20222027867777
22	22.53995308990439	29.425401021685982	25.14858176469419	22.88606412371544
23	21.550940189628097	29.818968772982966	25.793098650340895	22.836992387048046
24	19.996670575841303	31.435602973623013	25.672469140710408	22.89525730982528
25	22.023146951837642	30.93631358206285	24.261563537339242	22.77897592876026
26	23.280128803991335	29.795364646484725	22.9719086048222	23.952597944701743
27	21.927363891152677	28.96586594843865	24.947822457214414	24.158947703194258
28	22.15756624063289	29.204640322805066	27.154932517044667	21.482860919517382
29	22.747545170844184	30.14209684151941	24.701718380409073	22.408639607227336
30	22.55597904947425	30.436154564789604	23.747738973145957	23.260127412590194
31	23.05576537001332	30.897677353952574	23.086699198950487	22.959858077083624
32	22.385656641952732	29.870400922300185	23.942783597368265	23.801158838378818
33	21.826114611699694	30.241109940567295	24.164910850941183	23.767864596791828
34	20.352720189230556	30.210300343874852	25.259769623725376	24.177209843169216
35	21.71182094655032	30.07327217794033	25.770364149555746	22.444542725953607
36	24.298584746268062	30.707974716253556	22.958118825657436	22.035321711820945
37	21.52758452761931	31.34155916436423	23.518530481623568	23.61232582639289
38	22.50156532628357	30.691824524438964	25.004969289789102	21.801640859488362
39	20.89039734441154	29.457452940825696	25.624515494245564	24.027634220517204
40	22.53796537398875	29.927423522630146	25.422265399829058	22.112345703552048
41	20.862320857103104	28.4238406646922	27.458183426424693	23.25565505178
42	20.764550080502495	29.747659464509336	25.842418851497744	23.645371603490428
43	21.43006221550816	28.037105686855234	26.484575324494624	24.048256773141983
44	22.440194597388142	26.873422250491956	25.713590013715237	24.972793138404658
45	22.12464469578008	27.918836589874573	28.238113458824465	21.71840525552088
46	21.184206603192273	28.985494643105607	26.641480649585564	23.18881810411656
47	21.35403208173488	29.18178158977519	27.777584527619315	21.68660180087062
48	21.421117493887774	28.236001510664092	27.190338706792026	23.152542288656104
49	21.135259098769605	29.316822039794072	26.63104514102844	22.916873720407878
50	21.414781649406667	27.55446341608857	26.990945954004253	24.039808980500506
51	20.340918125981435	27.337305452304754	26.681980361366755	25.639796060347052
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19130.0
1	14309.0
2	9488.0
3	5896.5
4	2305.0
5	2089.5
6	1874.0
7	1969.5
8	2065.0
9	2106.0
10	2147.0
11	2148.0
12	2149.0
13	2272.0
14	2395.0
15	2758.5
16	3122.0
17	3315.5
18	3509.0
19	3470.0
20	3431.0
21	2998.5
22	2566.0
23	3508.5
24	4451.0
25	5390.5
26	6490.0
27	6650.0
28	9956.0
29	13262.0
30	13030.5
31	12799.0
32	15454.5
33	18110.0
34	21450.0
35	24790.0
36	25744.5
37	26699.0
38	27997.0
39	29295.0
40	30496.0
41	31697.0
42	31881.0
43	32065.0
44	39355.0
45	46645.0
46	48226.0
47	49807.0
48	55025.0
49	60243.0
50	58101.5
51	55960.0
52	52932.5
53	49905.0
54	48671.5
55	47438.0
56	43568.0
57	39698.0
58	38198.0
59	36698.0
60	35054.0
61	33410.0
62	30846.0
63	28282.0
64	26639.5
65	24997.0
66	22695.0
67	20393.0
68	17237.0
69	14081.0
70	12587.5
71	11094.0
72	9833.5
73	8573.0
74	7200.5
75	4992.0
76	4156.0
77	4027.5
78	3899.0
79	2808.5
80	1718.0
81	1361.0
82	1004.0
83	629.0
84	254.0
85	219.5
86	185.0
87	189.0
88	193.0
89	141.0
90	89.0
91	68.5
92	48.0
93	28.0
94	8.0
95	6.5
96	5.0
97	4.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	804944.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.6952780846855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.92328666820497	17.122626516240587
2	8.22036376790409	3.5668615580390464
3	3.0300607405726416	1.972140311406347
4	1.5290447268536655	1.3269220221204903
5	1.000612467452865	1.085428286819661
6	0.7052175145896381	0.917993405352777
7	0.49949177563008457	0.7585629081315614
8	0.3937408602478379	0.6833853965104106
9	0.3330209947441592	0.650248478011181
>10	3.24298011335216	16.45309235733618
>50	1.1837024684015112	18.64004199269198
>100	0.9297613890242602	32.070055271796896
>500	0.003486605208840976	0.46923793427796223
>1k	0.004648806945121301	2.1746064288985947
>5k	0.0	0.0
>10k+	5.811008681401626E-4	2.1087971323663286
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16727	2.0780327575582898	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3801	0.472206762209545	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2946	0.3659881929674611	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGT	2149	0.266975093919577	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCC	2015	0.2503279731260808	No Hit
AATCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCT	1837	0.22821463356457097	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1679	0.20858593889761276	No Hit
GCAGTCTGGGGTGTGATTGCTCTTAGGTGAGTTCCGAGTCTTAATCTATGC	1607	0.19964121727722672	No Hit
CTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGCT	1215	0.150942177344014	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	833	0.10348545985807708	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2423224472758352E-4	0.0	0.0	0.18821185076228905	0.0
2	1.2423224472758352E-4	0.0	0.0	0.27728637023196645	0.0
3	2.4846448945516704E-4	0.0	0.0	0.4245015802341529	0.0
4	2.4846448945516704E-4	0.0	0.0	1.067651911188853	0.0
5	2.4846448945516704E-4	0.0	0.0	1.1674104037051025	0.0
6	2.4846448945516704E-4	0.0	0.0	1.6698056013834504	0.0
7	2.4846448945516704E-4	0.0	0.0	2.0315698980301735	0.0
8	2.4846448945516704E-4	0.0	0.0	2.340162793933491	0.0
9	3.726967341827506E-4	0.0	0.0	3.0344968097159555	0.0
10	3.726967341827506E-4	0.0	0.0	3.812563358444811	0.0
11	3.726967341827506E-4	0.0	0.0	4.656597229124014	0.0
12	3.726967341827506E-4	0.0	0.0	4.970407879305889	0.0
13	3.726967341827506E-4	0.0	0.0	5.12669204317319	0.0
14	3.726967341827506E-4	0.0	0.0	5.296765986205251	0.0
15	3.726967341827506E-4	0.0	0.0	5.436651493768511	0.0
16	3.726967341827506E-4	0.0	0.0	5.686979466894591	0.0
17	3.726967341827506E-4	0.0	0.0	5.976689061599316	0.0
18	3.726967341827506E-4	0.0	0.0	6.42529169731062	0.0
19	3.726967341827506E-4	0.0	0.0	6.581700093422648	0.0
20	3.726967341827506E-4	0.0	0.0	6.815380945755233	0.0
21	3.726967341827506E-4	0.0	0.0	7.001977777336063	0.0
22	3.726967341827506E-4	0.0	0.0	7.213172593372955	0.0
23	3.726967341827506E-4	0.0	0.0	7.421510067781113	0.0
24	3.726967341827506E-4	0.0	0.0	7.576303444711682	0.0
25	3.726967341827506E-4	0.0	0.0	7.72240056451132	0.0
26	3.726967341827506E-4	0.0	0.0	7.859801427180027	0.0
27	3.726967341827506E-4	0.0	0.0	8.020806416346975	0.0
28	3.726967341827506E-4	0.0	0.0	8.168518555328072	0.0
29	3.726967341827506E-4	0.0	0.0	8.313249120435707	0.0
30	3.726967341827506E-4	0.0	0.0	8.518108091991493	0.0
31	3.726967341827506E-4	0.0	0.0	8.656875509352203	0.0
32	3.726967341827506E-4	0.0	0.0	8.805208809556937	0.0
33	3.726967341827506E-4	0.0	0.0	8.95267248404858	0.0
34	3.726967341827506E-4	0.0	0.0	9.09206106263293	0.0
35	3.726967341827506E-4	0.0	0.0	9.263750024846448	0.0
36	3.726967341827506E-4	0.0	0.0	9.410592538114452	0.0
37	3.726967341827506E-4	0.0	0.0	9.564888986066112	0.0
38	3.726967341827506E-4	0.0	0.0	9.718936969528315	0.0
39	3.726967341827506E-4	0.0	0.0	9.885035480729094	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCACG	50	2.1827873E-11	45.000004	1
CACGGTT	50	2.1827873E-11	45.000004	44
CAACGTT	25	3.8897597E-5	45.000004	38
TTTACGT	25	3.8897597E-5	45.000004	12
CATGCGC	25	3.8897597E-5	45.000004	35
CTATCAT	25	3.8897597E-5	45.000004	36
CACGCGA	25	3.8897597E-5	45.000004	11
CGTCTAC	25	3.8897597E-5	45.000004	5
TCGATAG	50	2.1827873E-11	45.000004	1
GTTACGC	25	3.8897597E-5	45.000004	9
CGCTACG	50	2.1827873E-11	45.000004	1
GTCCGTT	25	3.8897597E-5	45.000004	11
CACGTTA	25	3.8897597E-5	45.000004	33
CGTCATA	25	3.8897597E-5	45.000004	28
TAATGCG	25	3.8897597E-5	45.000004	1
CGATACG	25	3.8897597E-5	45.000004	1
GCCCGGT	25	3.8897597E-5	45.000004	26
TACGTTA	25	3.8897597E-5	45.000004	14
AAGTCGA	50	2.1827873E-11	45.000004	32
TCACGCG	40	6.8102963E-9	45.0	1
>>END_MODULE