FastQCFastQC Report
Sat 18 Jun 2016
SRR3548543_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548543_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554555
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA169273.052357295489176No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39070.7045288564705033No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33380.6019240652414999No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21180.3819278520615629No Hit
CTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGCT19390.3496497191441787TruSeq Adapter, Index 13 (95% over 24bp)
AAAAACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTT6380.11504720000721298No Hit
AGCTCTGGGAATGATACCTGTCTCTTATACACATCTGACGCCTGAACACTC6020.10855550847075582No Hit
GGGGAAGGGGAAGAAGATTGATCAAACTGAATGAAACACACACACACACAC5950.10729323511644472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG302.1634623E-645.0000041
GAGTGTA302.1634623E-645.00000410
TATGGAT302.1634623E-645.00000436
ATTCGCC302.1634623E-645.00000443
CGACATA302.1634623E-645.00000430
ATGTCCA302.1634623E-645.00000422
CCCACAT302.1634623E-645.00000412
TAGCACG600.045.0000041
GTATGGA302.1634623E-645.00000435
AAATTCG302.1634623E-645.00000413
ATGACTC302.1634623E-645.00000412
AATTCGT302.1634623E-645.00000414
GTATCAT302.1634623E-645.00000434
CGTAAGT302.1634623E-645.00000445
CGTCGAA302.1634623E-645.00000443
GCGTCTC302.1634623E-645.00000417
TATGTCC302.1634623E-645.00000421
CTTAACG406.8048394E-945.01
TTGATCG207.0301257E-445.014
TCACCGG351.2104101E-745.02