##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548543_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	554555
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.507368971517703	31.0	31.0	34.0	30.0	34.0
2	31.71728683358729	31.0	31.0	34.0	30.0	34.0
3	31.890422050112253	33.0	31.0	34.0	30.0	34.0
4	35.65999044278746	37.0	35.0	37.0	33.0	37.0
5	35.41299420255881	37.0	35.0	37.0	33.0	37.0
6	32.66488806340219	37.0	35.0	37.0	26.0	37.0
7	34.13550143809	37.0	35.0	37.0	27.0	37.0
8	35.16901118915166	37.0	35.0	37.0	32.0	37.0
9	36.92929105318679	39.0	37.0	39.0	33.0	39.0
10	36.560521499220094	38.0	35.0	39.0	32.0	39.0
11	36.5722426089387	38.0	35.0	39.0	32.0	39.0
12	36.52648339659727	38.0	35.0	39.0	32.0	39.0
13	36.50867091632029	39.0	35.0	39.0	32.0	39.0
14	37.597037264112664	39.0	36.0	41.0	32.0	41.0
15	37.67214252869418	39.0	36.0	41.0	32.0	41.0
16	37.59714545897161	39.0	36.0	41.0	32.0	41.0
17	37.53050463885458	39.0	36.0	41.0	32.0	41.0
18	37.34646518379601	39.0	36.0	40.0	32.0	41.0
19	37.17840069966009	39.0	35.0	40.0	32.0	41.0
20	37.01773854712337	39.0	35.0	40.0	31.0	41.0
21	36.94037561648529	39.0	35.0	40.0	31.0	41.0
22	36.862655642812705	39.0	35.0	40.0	31.0	41.0
23	36.63148831044712	39.0	35.0	40.0	31.0	41.0
24	36.51077891282199	38.0	35.0	40.0	30.0	41.0
25	36.49347494838204	38.0	35.0	40.0	30.0	41.0
26	36.41528613032071	38.0	35.0	40.0	30.0	41.0
27	36.29992696847022	38.0	35.0	40.0	30.0	41.0
28	36.14059921919377	38.0	35.0	40.0	30.0	41.0
29	35.90221348648917	38.0	35.0	40.0	29.0	41.0
30	35.957748104335906	38.0	35.0	40.0	30.0	41.0
31	35.49549097925364	38.0	34.0	40.0	27.0	41.0
32	35.55837022477482	38.0	34.0	40.0	27.0	41.0
33	35.4705358350389	38.0	34.0	40.0	27.0	41.0
34	35.39740332338542	38.0	34.0	40.0	27.0	41.0
35	35.359223160912805	38.0	34.0	40.0	27.0	41.0
36	35.21195553191298	38.0	34.0	40.0	26.0	41.0
37	35.12825238254096	38.0	34.0	40.0	26.0	41.0
38	34.96378357421717	38.0	34.0	40.0	25.0	41.0
39	34.810080154358	38.0	34.0	40.0	24.0	41.0
40	34.65810604899424	37.0	33.0	40.0	24.0	41.0
41	34.59604547790571	37.0	33.0	40.0	24.0	41.0
42	34.46217958543337	37.0	33.0	40.0	23.0	41.0
43	34.39116588976747	37.0	33.0	40.0	23.0	41.0
44	34.20463975620092	37.0	33.0	40.0	23.0	41.0
45	34.021204389104774	37.0	33.0	40.0	23.0	41.0
46	33.75809973762747	36.0	33.0	40.0	22.0	41.0
47	33.71327641081588	36.0	33.0	40.0	22.0	41.0
48	33.67084058389159	36.0	33.0	40.0	22.0	41.0
49	33.5736040609137	36.0	33.0	40.0	21.0	41.0
50	33.41682249731767	35.0	33.0	40.0	20.0	41.0
51	32.333068857011476	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	6.0
11	11.0
12	6.0
13	17.0
14	28.0
15	54.0
16	142.0
17	322.0
18	597.0
19	1153.0
20	1944.0
21	2849.0
22	3975.0
23	4898.0
24	5860.0
25	6249.0
26	6817.0
27	7845.0
28	9297.0
29	11169.0
30	13766.0
31	17249.0
32	22260.0
33	29616.0
34	42697.0
35	57675.0
36	58229.0
37	74167.0
38	93277.0
39	82364.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.384172895384587	33.815221213405344	17.97964133404261	26.82096455716746
2	23.984816654795285	34.0604628936715	16.67589328380413	25.27882716772908
3	19.92264067585722	35.46915995708271	19.051852386147452	25.556346980912625
4	18.689399608695258	36.9101351534113	18.40178160867723	25.998683629216217
5	16.75307228318201	39.03183633724338	15.811596685630821	28.40349469394379
6	17.41288059795692	46.59014885809343	17.88082336287654	18.116147181073114
7	78.6142041817313	13.104020340633483	4.636149705619822	3.6456257720154
8	79.07457330652505	12.908007321185455	4.0657824742361	3.951636898053394
9	72.69919124342941	15.583485858030313	6.97099476156558	4.746328136974691
10	36.3888162580808	35.506487183417335	12.407966748113353	15.69672981038851
11	25.755786170893778	36.026724130158414	20.00703266583116	18.210457033116644
12	25.017175933856876	29.329642686478348	26.091911532670338	19.561269846994435
13	24.184436169541343	29.620145882734803	26.055125280630413	20.140292667093433
14	20.457664253320228	33.69242004850736	25.939356781563593	19.91055891660881
15	20.769445771835073	32.1524465562478	26.556969101351534	20.52113857056559
16	21.703347729260397	33.08688948796783	24.435448242284355	20.774314540487417
17	21.23468366528117	31.944712427081175	25.02186437774432	21.79873952989334
18	24.624969570195923	30.269856010675227	23.862015489897306	21.243158929231544
19	22.365500265979026	33.93982562595234	23.041177160065278	20.653496948003355
20	21.687839799478862	33.278394388293314	23.933784746328136	21.099981065899687
21	23.014308770094942	32.18833118446322	25.433726140779545	19.363633904662297
22	20.934442931720028	31.12225117436503	23.557086312448718	24.38621958146622
23	20.49048336053232	33.295525240958966	23.106274400194753	23.10771699831396
24	20.103326090288608	32.53022693871663	24.577183507497004	22.789263463497758
25	22.34620551613456	32.49380133620651	23.084996077936363	22.07499706972257
26	22.62462695314261	32.12593881580727	23.29777930052024	21.951654930529884
27	21.231437819512944	30.694520832018462	23.82919638268522	24.244844965783376
28	21.62310320887919	32.118365175681404	24.241779444780047	22.016752170659355
29	22.513727222728132	33.71658356700418	23.2481899901723	20.52149922009539
30	22.9187366446971	31.084743623265503	23.095635239065558	22.900884492971844
31	22.096455716745858	33.68917420273913	20.77810136055035	23.436268719964655
32	22.856163951276248	31.71101153176872	22.545644706115713	22.887179810839324
33	21.815690057794086	31.63671772862926	22.95191640143899	23.59567581213766
34	21.592267674081018	31.545293072824155	23.785918439108837	23.07652081398599
35	21.159127588787406	29.418723120339727	25.749474804122226	23.67267448675064
36	22.95137542714429	31.256953773746517	24.43725148993337	21.354419309175825
37	21.61841476499175	32.68476526223729	21.236126263400383	24.460693709370577
38	22.524005734327524	32.34773827663622	23.82613086168189	21.302125127354365
39	22.297878479140934	29.444148912190855	23.263697919953838	24.994274688714373
40	22.567283677903905	29.925075060183392	23.408498706169812	24.09914255574289
41	20.59867821947327	29.80696233917285	25.22995915644075	24.364400284913128
42	22.253879236504943	28.663703329696784	25.868489148957273	23.213928284841
43	20.859247504756066	28.481755641911082	27.07341922802968	23.585577625303173
44	22.733002136848466	26.89886485560494	26.194696648664245	24.17343635888235
45	22.860491745633887	27.8136523879507	26.791210970958694	22.53464489545672
46	22.077701941196093	29.515557519091885	25.869931747076485	22.536808792635536
47	20.734102117914365	30.608505919160407	25.2126479790102	23.444743983915032
48	21.23342139192686	28.862421220618334	27.277186212368477	22.62697117508633
49	21.97185130419886	27.420183750935433	25.966585821063738	24.64137912380197
50	20.67910306461938	27.640360288880274	26.578247423609923	25.102289222890427
51	22.024686460315028	26.771375246819524	26.962158848085398	24.241779444780047
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19844.0
1	15393.0
2	10942.0
3	6962.5
4	2983.0
5	2571.5
6	2160.0
7	2373.5
8	2587.0
9	2896.0
10	3205.0
11	3082.0
12	2959.0
13	3084.5
14	3210.0
15	3172.0
16	3134.0
17	3202.5
18	3271.0
19	3144.0
20	3017.0
21	2733.0
22	2449.0
23	2792.0
24	3135.0
25	3480.0
26	4028.0
27	4231.0
28	6372.0
29	8513.0
30	8157.0
31	7801.0
32	8386.0
33	8971.0
34	9522.0
35	10073.0
36	11081.0
37	12089.0
38	11991.5
39	11894.0
40	14159.0
41	16424.0
42	17557.5
43	18691.0
44	23251.5
45	27812.0
46	32069.5
47	36327.0
48	41350.5
49	46374.0
50	45353.5
51	44333.0
52	40986.5
53	37640.0
54	34388.0
55	31136.0
56	29676.5
57	28217.0
58	26721.0
59	25225.0
60	23964.0
61	22703.0
62	21625.0
63	20547.0
64	18713.0
65	16879.0
66	15154.5
67	13430.0
68	12455.0
69	11480.0
70	9970.5
71	8461.0
72	7318.5
73	6176.0
74	5137.0
75	3664.0
76	3230.0
77	2509.0
78	1788.0
79	1475.5
80	1163.0
81	949.0
82	735.0
83	753.0
84	771.0
85	540.5
86	310.0
87	225.5
88	141.0
89	129.0
90	117.0
91	75.5
92	34.0
93	22.5
94	11.0
95	9.0
96	7.0
97	4.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	554555.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.84411319150113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3478035677793	21.300214204704645
2	8.402467849898954	4.511135961012734
3	3.1297362289693043	2.520449807699817
4	1.6362919812997436	1.7569922864142389
5	1.0401231880829689	1.396059229700212
6	0.7141265454732912	1.1502056291844425
7	0.5724711364980216	1.075723599091413
8	0.4543591697714713	0.9757495186353483
9	0.35018801147139655	0.8460437956420378
>10	2.871717776590097	16.913073261678015
>50	0.8892445471847834	17.431291329780368
>100	0.5839916406970405	24.338545912873187
>500	0.004079103427453134	0.6327270037936694
>1k	0.002719402284968756	2.0626133824274735
>5k	0.0	0.0
>10k+	6.79850571242189E-4	3.0891750773624
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16927	3.052357295489176	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3907	0.7045288564705033	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3338	0.6019240652414999	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2118	0.3819278520615629	No Hit
CTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTTCTGCT	1939	0.3496497191441787	TruSeq Adapter, Index 13 (95% over 24bp)
AAAAACTGTCTCTTATACACATCTGACGCCTGAACACTCGTATGCCGTCTT	638	0.11504720000721298	No Hit
AGCTCTGGGAATGATACCTGTCTCTTATACACATCTGACGCCTGAACACTC	602	0.10855550847075582	No Hit
GGGGAAGGGGAAGAAGATTGATCAAACTGAATGAAACACACACACACACAC	595	0.10729323511644472	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.606495298031755E-4	0.0	0.0	0.4298942395253852	0.0
2	3.606495298031755E-4	0.0	0.0	0.5483676100657284	0.0
3	3.606495298031755E-4	0.0	0.0	0.7081353517685351	0.0
4	3.606495298031755E-4	0.0	0.0	1.4359261029113433	0.0
5	3.606495298031755E-4	0.0	0.0	1.5697270784683215	0.0
6	3.606495298031755E-4	0.0	0.0	2.4098601581448187	0.0
7	3.606495298031755E-4	0.0	0.0	3.0393739124162615	0.0
8	3.606495298031755E-4	0.0	0.0	3.6789858535221933	0.0
9	3.606495298031755E-4	0.0	0.0	4.426432004039275	0.0
10	3.606495298031755E-4	0.0	0.0	5.812949121367583	0.0
11	3.606495298031755E-4	0.0	0.0	6.877586533346557	0.0
12	3.606495298031755E-4	0.0	0.0	7.433708108303054	0.0
13	3.606495298031755E-4	0.0	0.0	7.6906708982878165	0.0
14	3.606495298031755E-4	0.0	0.0	7.920043999242636	0.0
15	3.606495298031755E-4	0.0	0.0	8.103073635617747	0.0
16	3.606495298031755E-4	0.0	0.0	8.521246765424529	0.0
17	3.606495298031755E-4	0.0	0.0	8.962140815608912	0.0
18	3.606495298031755E-4	0.0	0.0	9.726176844496939	0.0
19	3.606495298031755E-4	0.0	0.0	9.940582989964927	0.0
20	3.606495298031755E-4	0.0	0.0	10.268774062085816	0.0
21	3.606495298031755E-4	0.0	0.0	10.537638286554085	0.0
22	3.606495298031755E-4	0.0	0.0	10.836256097231113	0.0
23	3.606495298031755E-4	0.0	0.0	11.137759104146568	0.0
24	3.606495298031755E-4	0.0	0.0	11.359197915445717	0.0
25	3.606495298031755E-4	0.0	0.0	11.548538918592385	0.0
26	3.606495298031755E-4	0.0	0.0	11.72399491484163	0.0
27	3.606495298031755E-4	0.0	0.0	11.96581042457466	0.0
28	3.606495298031755E-4	0.0	0.0	12.15551207725113	0.0
29	3.606495298031755E-4	0.0	0.0	12.34755795187132	0.0
30	3.606495298031755E-4	0.0	0.0	12.668175383866343	0.0
31	3.606495298031755E-4	0.0	0.0	12.851024695476553	0.0
32	3.606495298031755E-4	0.0	0.0	13.048660637808693	0.0
33	3.606495298031755E-4	0.0	0.0	13.238362290485163	0.0
34	3.606495298031755E-4	0.0	0.0	13.429326216515944	0.0
35	3.606495298031755E-4	0.0	0.0	13.633453850384543	0.0
36	3.606495298031755E-4	0.0	0.0	13.805303351335755	0.0
37	3.606495298031755E-4	0.0	0.0	13.980218373290295	0.0
38	3.606495298031755E-4	0.0	0.0	14.165051257314424	0.0
39	3.606495298031755E-4	0.0	0.0	14.368096942593612	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	30	2.1634623E-6	45.000004	1
GAGTGTA	30	2.1634623E-6	45.000004	10
TATGGAT	30	2.1634623E-6	45.000004	36
ATTCGCC	30	2.1634623E-6	45.000004	43
CGACATA	30	2.1634623E-6	45.000004	30
ATGTCCA	30	2.1634623E-6	45.000004	22
CCCACAT	30	2.1634623E-6	45.000004	12
TAGCACG	60	0.0	45.000004	1
GTATGGA	30	2.1634623E-6	45.000004	35
AAATTCG	30	2.1634623E-6	45.000004	13
ATGACTC	30	2.1634623E-6	45.000004	12
AATTCGT	30	2.1634623E-6	45.000004	14
GTATCAT	30	2.1634623E-6	45.000004	34
CGTAAGT	30	2.1634623E-6	45.000004	45
CGTCGAA	30	2.1634623E-6	45.000004	43
GCGTCTC	30	2.1634623E-6	45.000004	17
TATGTCC	30	2.1634623E-6	45.000004	21
CTTAACG	40	6.8048394E-9	45.0	1
TTGATCG	20	7.0301257E-4	45.0	14
TCACCGG	35	1.2104101E-7	45.0	2
>>END_MODULE