FastQCFastQC Report
Sat 18 Jun 2016
SRR3548540_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548540_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665362
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGCT53000.7965588657001752No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15650.23521030656995742No Hit
CCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGC11900.1788500094685299No Hit
AAACTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCT10140.15239824336225996No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTT7630.11467441783570448No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG750.045.0000041
GATACTA351.2109012E-745.0000049
TCGCACG351.2109012E-745.0000041
CCTAACG351.2109012E-745.0000041
CACTCCG351.2109012E-745.0000041
TAACACG700.045.0000041
CGGGTTG207.031142E-445.06
CTTCGAC207.031142E-445.033
AACGTAG253.889029E-545.01
TAATACG253.889029E-545.01
AACCGAT253.889029E-545.034
TCGTTCG253.889029E-545.045
ATTAGCG207.031142E-445.01
CTCAACG207.031142E-445.01
CGGCCTA207.031142E-445.045
GAGTACG207.031142E-445.01
CGGCACG253.889029E-545.01
CGCGCAA207.031142E-445.011
ATTAACG406.8084773E-945.01
ATTCGGT207.031142E-445.011