##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548540_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	665362
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86262365449184	33.0	31.0	34.0	30.0	34.0
2	32.06254189448751	34.0	31.0	34.0	30.0	34.0
3	32.20630423739258	34.0	31.0	34.0	30.0	34.0
4	35.832844376444704	37.0	35.0	37.0	35.0	37.0
5	35.64233154282932	37.0	35.0	37.0	33.0	37.0
6	35.646398501868156	37.0	35.0	37.0	33.0	37.0
7	35.76259089037246	37.0	35.0	37.0	35.0	37.0
8	35.94325044111326	37.0	35.0	37.0	35.0	37.0
9	37.52989801040636	39.0	37.0	39.0	35.0	39.0
10	37.06094877675611	39.0	37.0	39.0	33.0	39.0
11	37.06874152716867	39.0	37.0	39.0	33.0	39.0
12	37.07913887477794	39.0	37.0	39.0	33.0	39.0
13	37.06224581506007	39.0	37.0	39.0	33.0	39.0
14	38.25849838133227	40.0	38.0	41.0	33.0	41.0
15	38.2295442180347	40.0	38.0	41.0	33.0	41.0
16	38.17347849741945	40.0	37.0	41.0	33.0	41.0
17	38.17568331224206	40.0	37.0	41.0	33.0	41.0
18	38.144398087056366	40.0	37.0	41.0	33.0	41.0
19	38.137280157267774	40.0	37.0	41.0	33.0	41.0
20	38.08362966325098	40.0	37.0	41.0	33.0	41.0
21	38.03860154322008	40.0	37.0	41.0	33.0	41.0
22	38.03915612854356	40.0	37.0	41.0	33.0	41.0
23	37.91684226030341	40.0	37.0	41.0	33.0	41.0
24	37.83467796477707	40.0	37.0	41.0	33.0	41.0
25	37.8584439748588	40.0	37.0	41.0	33.0	41.0
26	37.786373432808006	40.0	37.0	41.0	33.0	41.0
27	37.72291925297808	40.0	37.0	41.0	33.0	41.0
28	37.69734370162408	40.0	37.0	41.0	33.0	41.0
29	37.55158996155476	40.0	37.0	41.0	32.0	41.0
30	37.487880281711305	40.0	36.0	41.0	32.0	41.0
31	37.45797776248118	40.0	36.0	41.0	32.0	41.0
32	37.31046858702481	39.0	36.0	41.0	31.0	41.0
33	37.32073818462732	39.0	36.0	41.0	32.0	41.0
34	37.272680135024245	39.0	36.0	41.0	31.0	41.0
35	37.179843453638775	39.0	36.0	41.0	31.0	41.0
36	37.10281771426682	39.0	36.0	41.0	31.0	41.0
37	37.001502941256035	39.0	36.0	41.0	31.0	41.0
38	36.96844875421199	39.0	36.0	41.0	31.0	41.0
39	36.90157538302458	39.0	35.0	41.0	31.0	41.0
40	36.84129691806866	39.0	35.0	41.0	31.0	41.0
41	36.78455787977071	39.0	35.0	41.0	31.0	41.0
42	36.71997799694001	39.0	35.0	41.0	31.0	41.0
43	36.61290545597735	39.0	35.0	40.0	30.0	41.0
44	36.49476074678145	39.0	35.0	40.0	30.0	41.0
45	36.314406293115624	39.0	35.0	40.0	30.0	41.0
46	36.15962137903878	39.0	35.0	40.0	29.0	41.0
47	36.082467889660066	38.0	35.0	40.0	29.0	41.0
48	36.00088072357604	38.0	35.0	40.0	29.0	41.0
49	35.83029238219195	38.0	35.0	40.0	28.0	41.0
50	35.68971026298466	38.0	34.0	40.0	28.0	41.0
51	34.37272642561493	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	1.0
12	5.0
13	3.0
14	12.0
15	18.0
16	31.0
17	80.0
18	174.0
19	333.0
20	574.0
21	931.0
22	1429.0
23	1931.0
24	2483.0
25	3132.0
26	4016.0
27	5243.0
28	6732.0
29	8652.0
30	11396.0
31	14728.0
32	19253.0
33	25724.0
34	36330.0
35	46545.0
36	59025.0
37	88024.0
38	142195.0
39	186306.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.956111109441174	30.785346923930128	20.46149314207905	27.79704882454964
2	22.867100916493577	30.528494263273227	20.800556689441237	25.803848130791962
3	20.941382285132004	31.52840108091535	20.58789050171185	26.942326132240797
4	19.72174545585711	32.04526257886684	19.969580468977792	28.263411496298257
5	18.534572157712645	33.68587325395799	18.187693315819057	29.5918612725103
6	20.83422257357649	36.28475927389902	19.53402809297796	23.34699005954653
7	85.23555598305884	4.409629645215687	5.505574409118645	4.849239962606822
8	86.96904842777316	3.9864013875153677	4.337187876674652	4.707362308036829
9	78.1027470760278	9.177560485870849	8.471027801407354	4.24866463669401
10	27.494957632085992	43.979217328311506	12.976545098758269	15.549279940844233
11	20.258295484262703	27.322119387641614	34.004947682614876	18.414637445480807
12	23.62563536841599	23.355707118831553	33.33764176493398	19.68101574781848
13	24.22651128258001	24.03353362530472	31.045205467099112	20.694749625016158
14	19.677558982929593	26.656166117091146	32.20758023451895	21.458694665460307
15	17.742972998157395	30.827278984973596	30.666163682326314	20.7635843345427
16	21.219426417499047	28.00385354138048	29.238670077341354	21.538049963779116
17	19.154535425828346	28.017981189187243	30.218738070403777	22.608745314580634
18	19.941325173364273	26.088505204685568	30.259918660819224	23.71025096113093
19	19.99017076418551	30.667967211833556	29.848413344916004	19.493448679064933
20	21.238814359701937	28.40604062149627	29.512956856568305	20.84218816223349
21	21.38820672055212	29.121741247621596	28.065474132878045	21.42457789894824
22	19.617140744436863	26.21159609355509	29.32043008166983	24.850833080338223
23	19.953198409286973	28.45744121245277	27.66929881778641	23.920061560473847
24	18.316194793210315	28.534842687138728	29.047495949573314	24.101466570077644
25	20.618700797460633	28.5850409250904	27.2029361460378	23.59332213141117
26	22.264421472822313	28.446319447158086	27.567249106501425	21.722009973518176
27	19.899994288823226	27.286048797496704	27.849651768510974	24.964305145169096
28	22.299890886464812	27.47286439562223	28.62336592711937	21.603878790793583
29	22.900917094754433	28.612544750075898	25.689624595333065	22.7969135598366
30	22.97260739266745	27.335645858945956	27.455881159429	22.23586558895759
31	24.97031691019325	26.230984035757977	25.242349277536135	23.556349776512636
32	22.55133295859998	29.92611540785317	25.95789960953586	21.56465202401099
33	22.78549120629071	27.34827056549668	27.323321740646445	22.542916487566167
34	22.36015883083194	27.583180283815427	26.57560846576751	23.481052419585126
35	23.31918564630983	27.764284705168013	28.098238252259673	20.818291396262488
36	22.980723275450053	28.505835920897194	27.518253221554584	20.995187582098165
37	22.13441705417502	30.27795395589168	25.874185781574543	21.713443208358758
38	22.02830940149873	29.39076773245241	27.81733853150616	20.7635843345427
39	20.48989873181817	26.916926425013756	28.228543259158172	24.364631584009906
40	21.84735527427175	26.69103435423123	28.157604431873175	23.304005939623842
41	20.12273018296807	26.769638181922023	29.57337509506103	23.534256540048876
42	22.9114376835467	27.583029989689823	27.113661435429137	22.391870891334342
43	20.994886993846958	26.44304904698495	28.87901623477145	23.683047724396644
44	23.71971949104397	25.60966812051184	26.471604930849672	24.19900745759451
45	23.724528903063295	25.296605456879114	28.964984474616823	22.01388116544077
46	20.59931285525774	25.73486312713981	30.80653839564027	22.85928562196218
47	21.102046705402472	26.550509346791674	28.075092956916688	24.27235099088917
48	21.41631172204003	25.44404399409645	30.956381638867263	22.183262644996258
49	21.385200838040046	25.114448976647296	28.8889356470613	24.61141453825136
50	19.992725764320777	24.427755116763507	28.400630032974533	27.178889085941186
51	20.537541969634574	23.63585536895705	31.122606941785076	24.703995719623304
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1972.0
1	1638.5
2	1305.0
3	919.5
4	534.0
5	522.5
6	511.0
7	550.0
8	589.0
9	686.5
10	784.0
11	819.5
12	855.0
13	940.5
14	1026.0
15	1198.0
16	1370.0
17	1345.0
18	1320.0
19	1582.5
20	1845.0
21	2149.5
22	2454.0
23	3145.5
24	3837.0
25	4230.0
26	6121.5
27	7620.0
28	8698.0
29	9776.0
30	11814.0
31	13852.0
32	15937.0
33	18022.0
34	19514.0
35	21006.0
36	22679.0
37	24352.0
38	26380.5
39	28409.0
40	30798.5
41	33188.0
42	35886.5
43	38585.0
44	42993.5
45	47402.0
46	55194.0
47	62986.0
48	68439.0
49	73892.0
50	69080.5
51	64269.0
52	57343.5
53	50418.0
54	43785.0
55	37152.0
56	32502.5
57	27853.0
58	25260.0
59	22667.0
60	20451.5
61	18236.0
62	16409.5
63	14583.0
64	12248.5
65	9914.0
66	8259.5
67	6605.0
68	5365.0
69	4125.0
70	3278.0
71	2431.0
72	2207.0
73	1983.0
74	1630.0
75	1004.0
76	731.0
77	548.0
78	365.0
79	336.0
80	307.0
81	235.0
82	163.0
83	98.0
84	33.0
85	65.0
86	97.0
87	57.0
88	17.0
89	14.5
90	12.0
91	8.0
92	4.0
93	3.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	665362.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.06273277880328
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.30605322764038	22.037802892512822
2	11.439074398554858	6.8777967376120905
3	4.178581555091628	3.768587420554676
4	1.987896592736834	2.3904641623736387
5	1.1902397063685668	1.789092911763975
6	0.7523758254799489	1.357108403438114
7	0.5679912799122315	1.195275904808431
8	0.46886797107499645	1.1276362018373827
9	0.3748369983392601	1.0141762065013713
>10	4.738524951939279	34.82436721566736
>50	0.9113835327207837	17.827367361815323
>100	0.08016567632501258	4.062957578975865
>500	0.002004141908125314	0.3613492708520465
>1k	0.0015031064310939857	0.5677054613761414
>5k	5.010354770313285E-4	0.7983122699107321
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGCT	5300	0.7965588657001752	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1565	0.23521030656995742	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCTGC	1190	0.1788500094685299	No Hit
AAACTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTCT	1014	0.15239824336225996	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTT	763	0.11467441783570448	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8963541651011029	0.0
2	0.0	0.0	0.0	1.2821591855260746	0.0
3	0.0	0.0	0.0	1.635500674820624	0.0
4	0.0	0.0	0.0	3.264688996365888	0.0
5	0.0	0.0	0.0	3.5388254814672315	0.0
6	0.0	0.0	0.0	5.118867623940051	0.0
7	0.0	0.0	0.0	6.263958566915454	0.0
8	0.0	0.0	0.0	7.3008377394561155	0.0
9	0.0	0.0	0.0	8.570522512557075	0.0
10	0.0	0.0	0.0	10.682154977290558	0.0
11	0.0	0.0	0.0	12.41234095124158	0.0
12	0.0	0.0	0.0	13.356638942410298	0.0
13	0.0	0.0	0.0	13.751912492748309	0.0
14	0.0	0.0	0.0	14.16732545591723	0.0
15	0.0	0.0	0.0	14.529233710371196	0.0
16	0.0	0.0	0.0	15.249292866139035	0.0
17	0.0	0.0	0.0	16.2547605664285	0.0
18	0.0	0.0	0.0	17.440581217442535	0.0
19	0.0	0.0	0.0	18.003583011954394	0.0
20	0.0	0.0	0.0	18.607314514504885	0.0
21	0.0	0.0	0.0	19.254481019354877	0.0
22	0.0	0.0	0.0	20.011362235895646	0.0
23	0.0	0.0	0.0	20.726311391392954	0.0
24	0.0	0.0	0.0	21.23851377145073	0.0
25	0.0	0.0	0.0	21.67121055906409	0.0
26	0.0	0.0	0.0	22.09999969941175	0.0
27	0.0	0.0	0.0	22.588605901749723	0.0
28	0.0	0.0	0.0	23.07766298646451	0.0
29	0.0	0.0	0.0	23.575587424589923	0.0
30	0.0	0.0	0.0	24.25491687231913	0.0
31	0.0	0.0	0.0	24.736008368376915	0.0
32	0.0	0.0	0.0	25.21830221743953	0.0
33	0.0	0.0	0.0	25.668884005999743	0.0
34	0.0	0.0	0.0	26.121569912318407	0.0
35	0.0	0.0	0.0	26.605817585013874	0.0
36	0.0	0.0	0.0	27.065567315235917	0.0
37	0.0	0.0	0.0	27.509235574018355	0.0
38	0.0	0.0	0.0	27.98957559944812	0.0
39	0.0	0.0	0.0	28.441510035138766	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	75	0.0	45.000004	1
GATACTA	35	1.2109012E-7	45.000004	9
TCGCACG	35	1.2109012E-7	45.000004	1
CCTAACG	35	1.2109012E-7	45.000004	1
CACTCCG	35	1.2109012E-7	45.000004	1
TAACACG	70	0.0	45.000004	1
CGGGTTG	20	7.031142E-4	45.0	6
CTTCGAC	20	7.031142E-4	45.0	33
AACGTAG	25	3.889029E-5	45.0	1
TAATACG	25	3.889029E-5	45.0	1
AACCGAT	25	3.889029E-5	45.0	34
TCGTTCG	25	3.889029E-5	45.0	45
ATTAGCG	20	7.031142E-4	45.0	1
CTCAACG	20	7.031142E-4	45.0	1
CGGCCTA	20	7.031142E-4	45.0	45
GAGTACG	20	7.031142E-4	45.0	1
CGGCACG	25	3.889029E-5	45.0	1
CGCGCAA	20	7.031142E-4	45.0	11
ATTAACG	40	6.8084773E-9	45.0	1
ATTCGGT	20	7.031142E-4	45.0	11
>>END_MODULE