FastQCFastQC Report
Sat 18 Jun 2016
SRR3548534_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548534_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867441
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGCT43840.5053946032064428No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG16780.1934425511360427No Hit
AATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCT12700.14640765193252336No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCC12190.14052828953208346No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGT10190.1174719663931034No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10120.1166649950832391No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAT207.0323277E-445.00000430
ACGTTAG302.164883E-645.0000041
GGCGTAC207.0323277E-445.0000048
CCTCGAA207.0323277E-445.00000420
ATTCACG302.164883E-645.0000041
CACTACG302.164883E-645.0000041
CGTACCG207.0323277E-445.0000041
ACATGCG207.0323277E-445.0000041
TTACGTC207.0323277E-445.0000048
TTACGTA207.0323277E-445.00000434
CATCGCG207.0323277E-445.0000041
TATACGC207.0323277E-445.00000418
AGAATCG207.0323277E-445.00000414
CGGAATA253.8900107E-545.010
AAACTCG253.8900107E-545.01
TACCACG351.2114651E-745.01
TAAACCG351.2114651E-745.01
CGTCTAA253.8900107E-545.031
CCGTCTA253.8900107E-545.037
TCGTCTA253.8900107E-545.030