##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548534_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	867441
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.919836622894238	33.0	31.0	34.0	30.0	34.0
2	32.137625498448884	34.0	31.0	34.0	30.0	34.0
3	32.26912147339127	34.0	31.0	34.0	30.0	34.0
4	35.87962985378833	37.0	35.0	37.0	35.0	37.0
5	35.689462453354174	37.0	35.0	37.0	33.0	37.0
6	35.693020044014524	37.0	35.0	37.0	33.0	37.0
7	35.73961572026224	37.0	35.0	37.0	35.0	37.0
8	35.88273092925052	37.0	35.0	37.0	35.0	37.0
9	37.49362319742784	39.0	37.0	39.0	35.0	39.0
10	37.09760663837656	39.0	37.0	39.0	33.0	39.0
11	37.05605222718317	39.0	37.0	39.0	33.0	39.0
12	36.93307556364064	39.0	37.0	39.0	33.0	39.0
13	36.88714160386701	39.0	37.0	39.0	33.0	39.0
14	38.084306598373836	40.0	37.0	41.0	33.0	41.0
15	38.081421099532996	40.0	37.0	41.0	33.0	41.0
16	38.014028619813914	40.0	37.0	41.0	33.0	41.0
17	37.99839527990952	40.0	37.0	41.0	33.0	41.0
18	37.97816566198738	40.0	37.0	41.0	33.0	41.0
19	37.959794383710246	40.0	37.0	41.0	33.0	41.0
20	37.91385927112046	40.0	37.0	41.0	33.0	41.0
21	37.860317877527116	40.0	37.0	41.0	33.0	41.0
22	37.84769684624084	40.0	37.0	41.0	33.0	41.0
23	37.734098342135084	40.0	36.0	41.0	33.0	41.0
24	37.60625564159407	39.0	36.0	41.0	32.0	41.0
25	37.61098218783756	39.0	36.0	41.0	32.0	41.0
26	37.56592897960783	39.0	36.0	41.0	33.0	41.0
27	37.48501166073543	39.0	36.0	41.0	32.0	41.0
28	37.4055757106247	39.0	36.0	41.0	32.0	41.0
29	37.2695410984724	39.0	36.0	41.0	32.0	41.0
30	37.25124014198084	39.0	36.0	41.0	32.0	41.0
31	37.161687077276724	39.0	35.0	41.0	31.0	41.0
32	37.115650516865124	39.0	35.0	41.0	31.0	41.0
33	37.026950536117155	39.0	35.0	41.0	31.0	41.0
34	36.92893810645335	39.0	35.0	41.0	31.0	41.0
35	36.90084858797313	39.0	35.0	41.0	31.0	41.0
36	36.704396033851296	39.0	35.0	41.0	30.0	41.0
37	36.62964397578625	39.0	35.0	41.0	30.0	41.0
38	36.51728359623306	39.0	35.0	40.0	30.0	41.0
39	36.46461488447053	39.0	35.0	40.0	30.0	41.0
40	36.35832754043214	38.0	35.0	40.0	30.0	41.0
41	36.283118967168946	38.0	35.0	40.0	30.0	41.0
42	36.18054945523672	38.0	35.0	40.0	30.0	41.0
43	36.066033309470036	38.0	35.0	40.0	29.0	41.0
44	35.94542798876235	38.0	35.0	40.0	29.0	41.0
45	35.77603087702795	38.0	34.0	40.0	29.0	41.0
46	35.62140825716101	38.0	34.0	40.0	28.0	41.0
47	35.5320880613206	38.0	34.0	40.0	28.0	41.0
48	35.46679140137485	38.0	34.0	40.0	28.0	41.0
49	35.29155066454087	37.0	34.0	40.0	28.0	41.0
50	35.116110490511744	37.0	34.0	40.0	27.0	41.0
51	33.92939346883534	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	4.0
11	10.0
12	13.0
13	10.0
14	22.0
15	35.0
16	67.0
17	125.0
18	261.0
19	498.0
20	843.0
21	1304.0
22	1945.0
23	2586.0
24	3534.0
25	4641.0
26	5936.0
27	7644.0
28	9845.0
29	12646.0
30	16588.0
31	20952.0
32	27140.0
33	36883.0
34	56664.0
35	69880.0
36	80803.0
37	116798.0
38	175716.0
39	213988.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.91508240906298	30.982049499620146	21.354535928091938	26.748332163224937
2	25.859395624601554	29.108377399730934	19.22551504943852	25.806711926228985
3	21.46416874461779	29.457795976902172	20.661347572918505	28.416687705561532
4	22.025936057899038	31.361787141719148	18.777300127616748	27.834976672765066
5	19.107351393351248	33.15672189808875	17.625636786824693	30.110289921735312
6	21.239254312397037	36.596379465577485	18.84012860817047	23.324237613855008
7	85.3515109384961	4.5502806530934095	5.313329667377954	4.784878741032531
8	86.77546945555952	4.096416932102587	4.359028452655569	4.76908515968233
9	78.72201106472947	7.733321344045301	8.446914545196734	5.09775304602849
10	35.946998124368115	33.69358838237989	12.714755239837638	17.644658253414352
11	27.660094461755904	28.75515452924176	24.153227712317033	19.431523296685306
12	27.40220948744641	23.72749270555577	28.927269981474247	19.94302782552358
13	25.963033797111272	24.738051348737265	27.42342130473427	21.875493549417193
14	22.106172062422687	27.010597838930835	28.924157377850484	21.959072720795998
15	21.161785066650065	28.285958353363515	27.869791720704924	22.682464859281495
16	23.782597317857928	26.199591672517208	26.801015861597506	23.21679514802736
17	22.139719012589907	26.753750399162595	28.077875037034218	23.02865555121328
18	25.045161572948476	25.50490465633974	26.03231804814391	23.417615722567874
19	22.462276973304235	29.350468792690222	25.62249190434854	22.564762329657004
20	25.828500151595325	26.687348188522332	25.586524040251728	21.897627619630615
21	24.95904620602439	27.484520561052566	24.892413432152736	22.664019800770312
22	23.390755106110962	25.110987375510263	25.479888545733946	26.018368972644822
23	23.427645223133332	28.214253188401283	24.171096362749743	24.187005225715637
24	21.025406915283	28.42487270027587	26.42658117382047	24.123139210620664
25	24.029069412213627	27.767536927583546	23.43871225824004	24.764681401962786
26	26.02839847321028	26.007301937538113	23.806691175538162	24.15760841371344
27	22.792328239038735	24.9542043781652	25.577762637458918	26.675704745337146
28	23.238468091778	28.164912656883867	26.34357841052014	22.25304084081799
29	23.549613172538535	29.012001969009997	23.03684054592762	24.40154431252385
30	26.641581387091456	25.103609352105792	25.142343974979276	23.112465285823475
31	25.35734418825027	27.752435035927515	22.158279352716782	24.731941423105432
32	24.310932962587657	27.43725509861766	24.459876810065467	23.791935128729218
33	23.498082290322916	26.722739644540667	24.094895214775413	25.684282850361008
34	21.490568234611924	28.75988107548525	25.52554006554913	24.224010624353703
35	25.378786568769517	26.271988527173605	25.09945921394078	23.2497656901161
36	23.64564275841239	31.469575452393883	23.423033958505535	21.461747830688196
37	23.787669708948506	30.89455075330772	21.39684428105197	23.92093525669181
38	23.897302525474355	29.999850133899596	23.84404241902331	22.25880492160274
39	24.03218201583739	25.93144663441087	24.40304297352788	25.633328376223858
40	26.0484574743412	25.778467930383737	25.171510223750087	23.001564371524978
41	23.83136144129687	24.488927777220585	26.934166127725113	24.74554465375743
42	25.75195315877391	25.839567186702034	24.268163483164848	24.140316171359206
43	23.088717272990326	25.286330712982206	25.686242637827817	25.938709376199647
44	25.180386908158596	24.456994769673095	24.938295515199304	25.424322806969002
45	26.64469399071522	24.012123014706475	25.76082984318242	23.58235315139589
46	22.759588260181385	25.35169538908122	27.422729615040105	24.4659867356973
47	23.757465925636442	27.20242644744715	25.25105453857957	23.789053088336843
48	22.531215379489787	26.22034236334229	27.575938882298622	23.6725033748693
49	24.06872628801267	25.2481724981872	26.08776850529316	24.595332708506977
50	22.610183286240794	24.627035152823073	26.59466176950363	26.1681197914325
51	22.819534700342732	23.633307625533035	27.472185428173212	26.074972245951024
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1388.0
1	1194.0
2	1000.0
3	730.0
4	460.0
5	440.0
6	420.0
7	432.5
8	445.0
9	502.0
10	559.0
11	678.5
12	798.0
13	841.5
14	885.0
15	931.5
16	978.0
17	1155.5
18	1333.0
19	1372.0
20	1411.0
21	1694.0
22	1977.0
23	2229.0
24	2481.0
25	3139.5
26	4610.5
27	5423.0
28	7509.5
29	9596.0
30	10448.0
31	11300.0
32	14426.5
33	17553.0
34	17877.5
35	18202.0
36	21160.0
37	24118.0
38	25549.5
39	26981.0
40	29492.0
41	32003.0
42	35700.5
43	39398.0
44	46437.5
45	53477.0
46	61540.0
47	69603.0
48	76108.0
49	82613.0
50	80551.5
51	78490.0
52	74759.5
53	71029.0
54	65159.5
55	59290.0
56	54649.0
57	50008.0
58	46416.5
59	42825.0
60	41108.0
61	39391.0
62	35573.0
63	31755.0
64	28239.5
65	24724.0
66	21221.0
67	17718.0
68	15594.5
69	13471.0
70	11818.5
71	10166.0
72	9267.5
73	8369.0
74	6808.5
75	4079.5
76	2911.0
77	2194.5
78	1478.0
79	1331.0
80	1184.0
81	797.0
82	410.0
83	443.0
84	476.0
85	352.5
86	229.0
87	133.0
88	37.0
89	27.0
90	17.0
91	11.0
92	5.0
93	6.5
94	8.0
95	5.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	867441.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.093177488377343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.52906905723165	18.70171157896646
2	10.372712418680418	5.2056862751568715
3	3.8907173368851695	2.9289138207469914
4	1.913170922280635	1.9203015007356221
5	1.0933169772715436	1.3717398480865532
6	0.7489602290941316	1.127627517623688
7	0.5293890803711017	0.929883790791264
8	0.42637028814295436	0.8559188252913394
9	0.3385712932239471	0.7646246598004158
>10	4.322173192087914	26.935719152339978
>50	1.5458470432437912	27.380381204065063
>100	0.28185990552290685	9.806133744183802
>500	0.005074400917761007	0.8464161337394728
>1k	0.0027678550460514583	1.2249419484725248
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGCT	4384	0.5053946032064428	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1678	0.1934425511360427	No Hit
AATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCT	1270	0.14640765193252336	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCC	1219	0.14052828953208346	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGT	1019	0.1174719663931034	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1012	0.1166649950832391	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5950837002170752	0.0
2	0.0	0.0	0.0	0.8223037647517237	0.0
3	0.0	0.0	0.0	1.0718884627311829	0.0
4	0.0	0.0	0.0	2.2400370745676077	0.0
5	0.0	0.0	0.0	2.4157262568866353	0.0
6	0.0	0.0	0.0	3.523006175636153	0.0
7	0.0	0.0	0.0	4.318910450393744	0.0
8	0.0	0.0	0.0	5.06997017664602	0.0
9	0.0	0.0	0.0	6.0555127092217225	0.0
10	0.0	0.0	0.0	7.5069082508205165	0.0
11	0.0	0.0	0.0	8.813394801490821	0.0
12	0.0	0.0	0.0	9.432111232925351	0.0
13	0.0	0.0	0.0	9.71017048998145	0.0
14	0.0	0.0	0.0	10.01266944956487	0.0
15	0.0	0.0	0.0	10.286463286840258	0.0
16	0.0	0.0	0.0	10.831284202614356	0.0
17	0.0	0.0	0.0	11.462912175006716	0.0
18	0.0	0.0	0.0	12.315073878223417	0.0
19	0.0	0.0	0.0	12.66068816207673	0.0
20	0.0	0.0	0.0	13.074549162421421	0.0
21	0.0	0.0	0.0	13.511812330752178	0.0
22	0.0	0.0	0.0	13.98919350134476	0.0
23	0.0	0.0	0.0	14.476604172502798	0.0
24	0.0	0.0	0.0	14.857148785911665	0.0
25	0.0	0.0	0.0	15.189620965575756	0.0
26	0.0	0.0	0.0	15.489699011229582	0.0
27	0.0	0.0	0.0	15.8215947828152	0.0
28	0.0	0.0	0.0	16.173895400378814	0.0
29	0.0	0.0	0.0	16.542796570602498	0.0
30	0.0	0.0	0.0	17.069748835943887	0.0
31	0.0	0.0	0.0	17.415593683028586	0.0
32	0.0	0.0	0.0	17.795100761896197	0.0
33	0.0	0.0	0.0	18.17299389814408	0.0
34	0.0	0.0	0.0	18.522066630468238	0.0
35	0.0	0.0	0.0	18.9140241238309	0.0
36	0.0	0.0	0.0	19.266555304625907	0.0
37	0.0	0.0	0.0	19.605252691537522	0.0
38	0.0	0.0	0.0	19.9764594940751	0.0
39	0.0	0.0	0.0	20.366341918355253	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAT	20	7.0323277E-4	45.000004	30
ACGTTAG	30	2.164883E-6	45.000004	1
GGCGTAC	20	7.0323277E-4	45.000004	8
CCTCGAA	20	7.0323277E-4	45.000004	20
ATTCACG	30	2.164883E-6	45.000004	1
CACTACG	30	2.164883E-6	45.000004	1
CGTACCG	20	7.0323277E-4	45.000004	1
ACATGCG	20	7.0323277E-4	45.000004	1
TTACGTC	20	7.0323277E-4	45.000004	8
TTACGTA	20	7.0323277E-4	45.000004	34
CATCGCG	20	7.0323277E-4	45.000004	1
TATACGC	20	7.0323277E-4	45.000004	18
AGAATCG	20	7.0323277E-4	45.000004	14
CGGAATA	25	3.8900107E-5	45.0	10
AAACTCG	25	3.8900107E-5	45.0	1
TACCACG	35	1.2114651E-7	45.0	1
TAAACCG	35	1.2114651E-7	45.0	1
CGTCTAA	25	3.8900107E-5	45.0	31
CCGTCTA	25	3.8900107E-5	45.0	37
TCGTCTA	25	3.8900107E-5	45.0	30
>>END_MODULE