##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548531_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	571354
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76651603034196	31.0	31.0	34.0	30.0	34.0
2	31.970643418966176	33.0	31.0	34.0	30.0	34.0
3	32.1246372651631	34.0	31.0	34.0	30.0	34.0
4	35.735451226385045	37.0	35.0	37.0	33.0	37.0
5	35.55008803648876	37.0	35.0	37.0	33.0	37.0
6	35.559521767590674	37.0	35.0	37.0	33.0	37.0
7	35.657830346860266	37.0	35.0	37.0	35.0	37.0
8	35.88308474255891	37.0	35.0	37.0	35.0	37.0
9	37.46534897804163	39.0	37.0	39.0	35.0	39.0
10	36.98786041578426	39.0	37.0	39.0	33.0	39.0
11	37.05327695264232	39.0	37.0	39.0	33.0	39.0
12	37.04113736842658	39.0	37.0	39.0	33.0	39.0
13	36.979767359640434	39.0	37.0	39.0	33.0	39.0
14	38.13281083181355	40.0	37.0	41.0	33.0	41.0
15	38.11897002558834	40.0	37.0	41.0	33.0	41.0
16	38.07131480658226	40.0	37.0	41.0	33.0	41.0
17	38.063459781501486	40.0	37.0	41.0	33.0	41.0
18	38.01273641210178	40.0	37.0	41.0	33.0	41.0
19	37.997350154195125	40.0	37.0	41.0	33.0	41.0
20	37.942443738907926	40.0	37.0	41.0	33.0	41.0
21	37.93344756490722	40.0	37.0	41.0	33.0	41.0
22	37.92227585699934	40.0	37.0	41.0	33.0	41.0
23	37.802225940485236	39.0	37.0	41.0	33.0	41.0
24	37.73736947671671	39.0	37.0	41.0	33.0	41.0
25	37.743556534127706	39.0	37.0	41.0	33.0	41.0
26	37.68258907787466	39.0	37.0	41.0	33.0	41.0
27	37.65665069291543	40.0	37.0	41.0	33.0	41.0
28	37.61569359801454	39.0	37.0	41.0	32.0	41.0
29	37.475995617428076	39.0	36.0	41.0	32.0	41.0
30	37.49943992691046	39.0	36.0	41.0	32.0	41.0
31	37.37677516915957	39.0	36.0	41.0	32.0	41.0
32	37.28844464202579	39.0	36.0	41.0	31.0	41.0
33	37.29831067954368	39.0	36.0	41.0	32.0	41.0
34	37.1641784252845	39.0	36.0	41.0	31.0	41.0
35	37.12757939911158	39.0	36.0	41.0	31.0	41.0
36	37.05187676991847	39.0	36.0	41.0	31.0	41.0
37	36.88227788726429	39.0	35.0	41.0	31.0	41.0
38	36.794001617211045	39.0	35.0	40.0	30.0	41.0
39	36.66062196116593	39.0	35.0	40.0	30.0	41.0
40	36.59303689131432	39.0	35.0	40.0	30.0	41.0
41	36.5517770068994	39.0	35.0	40.0	30.0	41.0
42	36.495997227638206	39.0	35.0	40.0	30.0	41.0
43	36.443273347171804	39.0	35.0	40.0	30.0	41.0
44	36.39464325094425	39.0	35.0	40.0	30.0	41.0
45	36.19245686562096	39.0	35.0	40.0	29.0	41.0
46	35.98172761545381	38.0	35.0	40.0	28.0	41.0
47	35.88030888030888	38.0	35.0	40.0	28.0	41.0
48	35.82663287559026	38.0	35.0	40.0	28.0	41.0
49	35.69944552764136	38.0	34.0	40.0	27.0	41.0
50	35.518106112847725	38.0	34.0	40.0	27.0	41.0
51	34.22738267343888	37.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	2.0
13	1.0
14	2.0
15	7.0
16	33.0
17	65.0
18	138.0
19	244.0
20	499.0
21	853.0
22	1201.0
23	1643.0
24	2230.0
25	2821.0
26	3662.0
27	4643.0
28	5936.0
29	7916.0
30	10538.0
31	13655.0
32	17719.0
33	23008.0
34	32674.0
35	42456.0
36	52440.0
37	76003.0
38	120224.0
39	150700.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.599152189360712	34.47372382095863	19.90079705401555	27.026326935665107
2	21.084301501345927	32.531320337304024	20.530704256905526	25.85367390444453
3	20.409238405611934	31.23615131774697	22.150715668394724	26.203894608246376
4	21.509957049394945	30.873153946590033	19.976406921103205	27.64048208291182
5	17.903611421290478	34.8291952099749	18.46560976207395	28.801583606660667
6	20.812316007238945	35.511609265009085	20.302824518599678	23.373250209152292
7	82.3330194590394	5.478739975566811	6.679221638423814	5.509018926969969
8	84.17548490077955	4.171844425697554	5.053259450358272	6.599411223164623
9	75.25072021898858	9.256432964501867	10.395831656031113	5.097015160478442
10	26.027646607882325	45.09183448440021	12.90460905148122	15.975909856236239
11	19.469715797911626	25.663774122522987	35.801972157366535	19.064537922198845
12	23.359948473275765	22.60805035057075	35.823850012426625	18.208151163726864
13	24.35915386957998	24.50319766729558	30.78389229794488	20.353756165179558
14	18.341693591013627	27.456708100407102	33.37615558830427	20.825442720274996
15	16.536508014295865	31.24017684307802	31.326113057754036	20.897202084872077
16	20.31175068346419	28.238535128834314	29.51147624765032	21.938237940051177
17	18.741095712990546	28.161175033341852	31.11888601462491	21.978843239042696
18	18.395075557360236	26.765542903348887	30.760789283001433	24.078592256289447
19	18.559422004571598	31.05255235808273	31.33713949670432	19.050886140641353
20	20.246817209645858	29.77558571393567	29.58796122894038	20.389635847478097
21	20.06496847838643	28.76097830766915	28.535373866289547	22.638679347654868
22	18.81915589984493	25.9028553226196	29.795013249229026	25.482975528306444
23	19.194404869835513	28.444186966399116	27.19592406809089	25.165484095674483
24	16.523556324100294	30.17778820136028	29.357456148027318	23.941199326512113
25	19.340548941636886	28.18357795692338	28.074713750144394	24.40115935129534
26	20.574459967025696	28.823286438880274	27.2963871785268	23.30586641556723
27	17.601171952939858	26.853229346429707	28.708471455524947	26.837127245105485
28	20.618040654305386	26.208270179258392	31.351316346783253	21.822372819652966
29	20.93395688137302	27.838082869814514	26.575293075746387	24.652667173066085
30	22.06197908827102	25.367810499270156	27.876762917560743	24.693447494898084
31	22.27253156536928	28.44121157811094	25.039292627687914	24.246964228831864
32	19.31114510443613	29.71222744568166	25.316178761328352	25.660448688553856
33	22.517038473520795	26.129334878201604	27.239504755370575	24.114121892907026
34	18.18627330866678	28.100792153376013	28.60205756851269	25.11087696944451
35	21.052097298697483	27.365521200516667	27.357995218375997	24.224386282409853
36	21.373789279500972	27.741120216188214	28.03270826842903	22.852382235881784
37	21.63422326613623	29.962160061888078	24.427587800207927	23.976028871767767
38	22.79479972136364	29.96023481064279	26.271103378990958	20.973862089002616
39	22.181869734000287	27.98230169037059	26.09590551566979	23.739923059959324
40	21.732411079645892	30.52485849403347	25.655898094701357	22.086832331619277
41	19.225208889760115	27.18192224085243	30.610269640188044	22.982599229199412
42	21.202266895829904	28.309419379228988	27.29988763533641	23.188426089604693
43	19.989883679820217	24.82278937401331	28.865466943436118	26.321860002730357
44	21.47040188744631	24.69974831715539	27.321590467556018	26.508259327842282
45	24.14125043318153	25.093724731077405	27.959373698267626	22.80565113747344
46	19.477241780052296	26.224722326263578	31.62190165816639	22.676134235517736
47	20.648669651389508	26.364740598648122	28.224183255914898	24.76240649404747
48	20.438642242812687	25.732208053150934	31.004246054110062	22.824903649926316
49	20.863772722340265	24.070891251308296	29.404712314957106	25.660623711394337
50	20.544531061303502	23.475113502312052	27.333316997868224	28.647038438516226
51	20.006160803984923	21.978843239042696	32.59712892532475	25.417867031647628
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1947.0
1	1566.0
2	1185.0
3	828.0
4	471.0
5	417.5
6	364.0
7	377.5
8	391.0
9	495.0
10	599.0
11	599.5
12	600.0
13	641.0
14	682.0
15	771.5
16	861.0
17	996.5
18	1132.0
19	1381.5
20	1631.0
21	1974.0
22	2317.0
23	2419.0
24	2521.0
25	3756.5
26	5492.0
27	5992.0
28	7004.5
29	8017.0
30	9778.0
31	11539.0
32	13644.5
33	15750.0
34	16702.5
35	17655.0
36	19429.5
37	21204.0
38	23306.0
39	25408.0
40	26612.5
41	27817.0
42	30810.5
43	33804.0
44	42160.0
45	50516.0
46	54374.0
47	58232.0
48	62815.5
49	67399.0
50	61584.0
51	55769.0
52	48776.5
53	41784.0
54	35780.5
55	29777.0
56	25882.5
57	21988.0
58	19434.5
59	16881.0
60	15510.0
61	14139.0
62	11953.0
63	9767.0
64	8539.5
65	7312.0
66	5847.0
67	4382.0
68	3455.5
69	2529.0
70	2009.5
71	1490.0
72	1279.0
73	1068.0
74	862.0
75	559.5
76	463.0
77	310.5
78	158.0
79	109.5
80	61.0
81	56.0
82	51.0
83	35.5
84	20.0
85	17.0
86	14.0
87	13.0
88	12.0
89	9.0
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	571354.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.752365072367493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.27842180885796	20.781755707028523
2	11.729291836860591	6.744897618675114
3	4.705357043483901	4.0587043053025464
4	2.3251529293871473	2.674145834992959
5	1.4027329835586755	2.0165945421165157
6	0.8687964723888327	1.4987972008625257
7	0.6328997623895055	1.273815551570639
8	0.5042426574027519	1.159853517656372
9	0.3360181572465147	0.8695185055286379
>10	3.9534767357510137	26.957181176540914
>50	1.1056584110889467	21.70664528935201
>100	0.14754356427045193	6.070784436112169
>500	0.004897711677027449	0.787892343308921
>1k	0.004897711677027449	2.3325204292195068
>5k	6.122139596284311E-4	1.0668935417326564
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT	6061	1.060813436153418	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCC	2655	0.4646856414762126	No Hit
AATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	2210	0.38680047746230883	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGT	2183	0.3820748607693304	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1579	0.276361065118998	No Hit
CCCGACGGGATAACAGATAACCAACCATTCCCTTAAAAATCTCACATGCAC	1296	0.2268296012629648	No Hit
AAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	1271	0.22245403025094776	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTT	1051	0.18394900534519756	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	1006	0.17607297752356685	No Hit
AATGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTT	649	0.11358982347196309	No Hit
ACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTG	616	0.10781406973610055	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	578	0.10116320179783461	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7502284048068274E-4	0.0	0.0	1.2391617106032338	0.0
2	1.7502284048068274E-4	0.0	0.0	1.6765437889644599	0.0
3	1.7502284048068274E-4	0.0	0.0	2.1874354603275727	0.0
4	1.7502284048068274E-4	0.0	0.0	4.580697781060429	0.0
5	1.7502284048068274E-4	0.0	0.0	4.911140903887957	0.0
6	1.7502284048068274E-4	0.0	0.0	6.7921113705338545	0.0
7	1.7502284048068274E-4	0.0	0.0	8.203145510489119	0.0
8	1.7502284048068274E-4	0.0	0.0	9.403977219027084	0.0
9	1.7502284048068274E-4	0.0	0.0	11.329228464314593	0.0
10	1.7502284048068274E-4	0.0	0.0	13.756270193260221	0.0
11	1.7502284048068274E-4	0.0	0.0	16.176661054267583	0.0
12	1.7502284048068274E-4	0.0	0.0	17.28315545178646	0.0
13	1.7502284048068274E-4	0.0	0.0	17.728413557969315	0.0
14	1.7502284048068274E-4	0.0	0.0	18.2268786076583	0.0
15	1.7502284048068274E-4	0.0	0.0	18.6726617823626	0.0
16	1.7502284048068274E-4	0.0	0.0	19.52677324390833	0.0
17	1.7502284048068274E-4	0.0	0.0	20.67509810030209	0.0
18	1.7502284048068274E-4	0.0	0.0	22.177144117307307	0.0
19	1.7502284048068274E-4	0.0	0.0	22.80232570350431	0.0
20	1.7502284048068274E-4	0.0	0.0	23.502067019746075	0.0
21	1.7502284048068274E-4	0.0	0.0	24.16942911049892	0.0
22	1.7502284048068274E-4	0.0	0.0	24.95930718958824	0.0
23	1.7502284048068274E-4	0.0	0.0	25.69965380482153	0.0
24	1.7502284048068274E-4	0.0	0.0	26.221396892294443	0.0
25	1.7502284048068274E-4	0.0	0.0	26.700959475211516	0.0
26	1.7502284048068274E-4	0.0	0.0	27.144117307308605	0.0
27	1.7502284048068274E-4	0.0	0.0	27.64800806505249	0.0
28	1.7502284048068274E-4	0.0	0.0	28.146823160422436	0.0
29	1.7502284048068274E-4	0.0	0.0	28.67591720719554	0.0
30	1.7502284048068274E-4	0.0	0.0	29.38878523647336	0.0
31	1.7502284048068274E-4	0.0	0.0	29.892675994217246	0.0
32	1.7502284048068274E-4	0.0	0.0	30.359986978300668	0.0
33	1.7502284048068274E-4	0.0	0.0	30.84269997234639	0.0
34	1.7502284048068274E-4	0.0	0.0	31.31631177868712	0.0
35	1.7502284048068274E-4	0.0	0.0	31.831228975381286	0.0
36	1.7502284048068274E-4	0.0	0.0	32.29556457117654	0.0
37	1.7502284048068274E-4	0.0	0.0	32.76935140035775	0.0
38	1.7502284048068274E-4	0.0	0.0	33.25959037654414	0.0
39	1.7502284048068274E-4	0.0	0.0	33.72620126926564	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTA	25	3.8883336E-5	45.000004	1
TCGCACA	25	3.8883336E-5	45.000004	35
CGACGTT	25	3.8883336E-5	45.000004	19
TTCGTAC	25	3.8883336E-5	45.000004	33
CGCGAAG	50	2.1827873E-11	45.000004	1
TCTAGCG	50	2.1827873E-11	45.000004	1
ATCGTTC	25	3.8883336E-5	45.000004	10
TCTCGCG	25	3.8883336E-5	45.000004	1
CTCGCAC	25	3.8883336E-5	45.000004	34
TCGTACA	25	3.8883336E-5	45.000004	34
ATCGCTC	25	3.8883336E-5	45.000004	10
TCAACGA	25	3.8883336E-5	45.000004	37
AATCGAC	25	3.8883336E-5	45.000004	16
AGCGTTC	20	7.0303044E-4	45.0	41
AACGTAC	20	7.0303044E-4	45.0	19
CCTTATC	20	7.0303044E-4	45.0	42
CGACGGA	20	7.0303044E-4	45.0	3
CGTGCAA	20	7.0303044E-4	45.0	26
GACACGC	45	3.8380676E-10	45.0	45
CGGCCGA	20	7.0303044E-4	45.0	17
>>END_MODULE