SRR3548527_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548527_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	780645
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCT	4202	0.5382728384861236	TruSeq Adapter, Index 3 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	3892	0.4985620864797699	TruSeq Adapter, Index 3 (95% over 24bp)
AATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGT	2381	0.3050041952488007	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCC	2370	0.3035951040485752	No Hit
AACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTG	1092	0.1398843264223815	RNA PCR Primer, Index 3 (95% over 22bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1046	0.1339917632214387	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	883	0.11311159361809785	RNA PCR Primer, Index 3 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	25	3.8896516E-5	45.0	1
CCGATAC	20	7.031894E-4	45.0	37
ACGATAC	20	7.031894E-4	45.0	11
CGTAGCG	40	6.8102963E-9	45.0	1
TCATACG	40	6.8102963E-9	45.0	1
CGGTATG	25	3.8896516E-5	45.0	1
CGACTCG	25	3.8896516E-5	45.0	1
CGATATG	20	7.031894E-4	45.0	1
CAATACG	25	3.8896516E-5	45.0	1
CTAGCGG	170	0.0	42.35294	2
TAGGGTA	135	0.0	41.666664	5
CGTAAAG	65	0.0	41.53846	1
AAACGCG	50	1.0804797E-9	40.5	1
CGATAGG	100	0.0	40.5	2
CATACGA	185	0.0	40.135136	18
CGCATAG	45	1.9268555E-8	40.0	1
TATCGCG	45	1.9268555E-8	40.0	1
ACGGGAT	740	0.0	39.527027	5
GTAACGG	80	0.0	39.375	2
CCGATCG	35	6.245713E-6	38.571426	26