##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548527_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	780645
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.010221035169636	33.0	31.0	34.0	30.0	34.0
2	32.207228637857156	34.0	31.0	34.0	30.0	34.0
3	32.31847126414696	34.0	31.0	34.0	30.0	34.0
4	35.89739125979158	37.0	35.0	37.0	35.0	37.0
5	35.72793779502847	37.0	35.0	37.0	35.0	37.0
6	35.72346200897975	37.0	35.0	37.0	33.0	37.0
7	35.73839068975014	37.0	35.0	37.0	35.0	37.0
8	35.8899960929744	37.0	35.0	37.0	35.0	37.0
9	37.50533084820885	39.0	37.0	39.0	35.0	39.0
10	37.15467978402475	39.0	37.0	39.0	34.0	39.0
11	37.15593899916095	39.0	37.0	39.0	34.0	39.0
12	37.08674621626988	39.0	37.0	39.0	33.0	39.0
13	37.04004893389441	39.0	37.0	39.0	33.0	39.0
14	38.288964894414235	40.0	38.0	41.0	33.0	41.0
15	38.27915633866866	40.0	38.0	41.0	33.0	41.0
16	38.235096618821615	40.0	38.0	41.0	33.0	41.0
17	38.20305388492849	40.0	37.0	41.0	33.0	41.0
18	38.152019163640325	40.0	37.0	41.0	33.0	41.0
19	38.15268271749643	40.0	37.0	41.0	34.0	41.0
20	38.09854287159977	40.0	37.0	41.0	33.0	41.0
21	38.09078006007852	40.0	37.0	41.0	33.0	41.0
22	38.062763484042044	40.0	37.0	41.0	33.0	41.0
23	37.97854082201257	40.0	37.0	41.0	33.0	41.0
24	37.87402468471584	40.0	37.0	41.0	33.0	41.0
25	37.85073625015212	40.0	37.0	41.0	33.0	41.0
26	37.7899698326384	40.0	37.0	41.0	33.0	41.0
27	37.718284239314926	40.0	37.0	41.0	33.0	41.0
28	37.624016038019846	40.0	37.0	41.0	33.0	41.0
29	37.456790218345084	40.0	36.0	41.0	32.0	41.0
30	37.47117447751539	40.0	36.0	41.0	32.0	41.0
31	37.44475786048716	40.0	36.0	41.0	32.0	41.0
32	37.400020495872006	40.0	36.0	41.0	32.0	41.0
33	37.355422759384865	40.0	36.0	41.0	32.0	41.0
34	37.33163729992506	39.0	36.0	41.0	32.0	41.0
35	37.243964926439034	39.0	36.0	41.0	32.0	41.0
36	37.162224826905955	39.0	36.0	41.0	31.0	41.0
37	37.08301084359728	39.0	35.0	41.0	31.0	41.0
38	37.04855728275977	39.0	35.0	41.0	31.0	41.0
39	36.997655784639626	39.0	35.0	41.0	31.0	41.0
40	36.86743910484279	39.0	35.0	41.0	31.0	41.0
41	36.82599517066016	39.0	35.0	41.0	31.0	41.0
42	36.75147218005623	39.0	35.0	41.0	31.0	41.0
43	36.6693900556591	39.0	35.0	40.0	31.0	41.0
44	36.602099545888336	39.0	35.0	40.0	31.0	41.0
45	36.44521133165524	39.0	35.0	40.0	30.0	41.0
46	36.28662836500586	38.0	35.0	40.0	30.0	41.0
47	36.164330777754294	38.0	35.0	40.0	30.0	41.0
48	36.12894337374863	38.0	35.0	40.0	30.0	41.0
49	35.98947280774232	38.0	35.0	40.0	30.0	41.0
50	35.80716586924915	38.0	35.0	40.0	29.0	41.0
51	34.543778542103006	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	4.0
11	13.0
12	9.0
13	20.0
14	28.0
15	31.0
16	68.0
17	116.0
18	223.0
19	401.0
20	660.0
21	1023.0
22	1451.0
23	1939.0
24	2710.0
25	3422.0
26	4414.0
27	5719.0
28	7394.0
29	9484.0
30	12400.0
31	16392.0
32	21690.0
33	29930.0
34	45025.0
35	55999.0
36	70103.0
37	102817.0
38	165964.0
39	221116.0
40	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.889777043342363	32.20618847235299	20.56542986889047	26.338604615414173
2	26.20307566179249	29.785369790365657	19.541148665526585	24.470405882315266
3	21.733054077077288	28.937224987029957	21.70231026907237	27.627410666820385
4	22.861736128457878	30.466345137674615	19.047710547047632	27.62420818681987
5	19.121367587059417	33.46591600535454	17.239206041158273	30.173510366427763
6	21.231801907397088	36.523515810643765	19.933644614389383	22.311037667569767
7	84.09866200385578	5.24694323283951	5.939831805750373	4.71456295755433
8	85.0993729544159	3.9203479174272555	4.933100192789296	6.047178935367549
9	77.48541270359766	7.931261969269002	8.62581583178013	5.957509495353201
10	33.85584997021694	34.25654426788105	14.453176539912507	17.434429221989507
11	26.095984730575356	28.346943873335512	25.775992928924158	19.781078467164974
12	27.72835283643654	22.65293443242447	30.362969083258072	19.25574364788092
13	26.79924933868788	23.910099981425617	27.518270148402923	21.77238053148358
14	21.628397030660544	27.379282516380687	28.72637370379622	22.265946749162552
15	19.985140492797623	28.772489415803598	28.727270398196364	22.515099693202416
16	21.74881027867981	26.040261578566444	28.861646459017866	23.349281683735885
17	21.25844654100135	25.5893523944943	28.311716593329873	24.840484471174477
18	22.806909670849105	25.373889540059825	26.883922909901425	24.935277879189645
19	23.7143644037943	28.665910881386548	26.180786400988925	21.43893831383023
20	25.462021789673923	26.220240954595237	25.701311095312207	22.61642616041863
21	25.23541430483767	26.19474921379116	25.927918580148468	22.64191790122271
22	22.405574877184893	25.870914436139348	25.823902029731826	25.89960865694394
23	23.62700074938032	27.624976782019996	24.481166215116986	24.266856253482697
24	21.739459037078312	27.45511724279282	25.976340077756216	24.829083642372655
25	22.553529453208565	26.34885255141582	25.21299694483408	25.88462105054154
26	25.144207674423075	26.2156293833945	24.500765392720123	24.139397549462306
27	22.494860019599177	25.251939101640307	26.661542698665848	25.59165818009467
28	24.222662029475625	26.214348391394292	27.077352701932377	22.485636877197702
29	24.41429843270629	28.08459671169354	24.976653920796267	22.524450934803912
30	25.075290304812047	24.11800498305888	27.625745377220117	23.180959334908955
31	25.87078633693933	25.33955895445433	24.513831511122213	24.27582319748413
32	24.069711584651156	26.01118306016179	26.06869960097099	23.850405754216062
33	24.129533911060726	26.147736807383637	26.630030295460806	23.09269898609483
34	22.908876634065418	25.61574082969852	27.68531150522965	23.79007103100641
35	25.800075578528016	23.56205445496993	27.5893652044143	23.04850476208776
36	25.256038276040965	26.563803009050208	26.963216314714117	21.21694240019471
37	24.129405811860703	28.029257857284684	24.86136464077782	22.979971690076795
38	24.789372890366298	25.837480544934	26.3806211530209	22.992525411678805
39	23.135356019701657	24.45849265671336	26.20307566179249	26.20307566179249
40	24.181670285469067	25.368637472858985	27.591670990014666	22.858021251657284
41	22.800632810048103	24.330009159092803	30.246398811239423	22.622959219619673
42	25.40117466966419	25.448699472871795	25.76676978652268	23.383356070941336
43	23.808645415009384	24.750174535160028	27.00907582832145	24.43210422150914
44	24.318608330290978	23.645575133383293	26.944513831511124	25.09130270481461
45	25.436401949669822	23.984397517437504	26.947460113111593	23.631740419781078
46	21.582793715453246	24.770542307963286	29.485489563117678	24.161174413465787
47	23.46457096375433	26.10905084897745	27.115654362738507	23.310723824529717
48	23.14112048370258	23.982219831037156	28.98167540943707	23.894984275823198
49	23.34736019573558	23.992083469438732	27.59730735481557	25.063248980010123
50	22.427351741188374	24.15963722306554	27.061468401129833	26.351542634616248
51	22.781033632444966	23.290612250126497	29.37263416789962	24.555719949528914
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	775.0
1	666.0
2	557.0
3	425.0
4	293.0
5	290.0
6	287.0
7	300.5
8	314.0
9	319.5
10	325.0
11	427.5
12	530.0
13	648.5
14	767.0
15	850.0
16	933.0
17	918.0
18	903.0
19	965.0
20	1027.0
21	1171.0
22	1315.0
23	2238.5
24	3162.0
25	3829.5
26	5018.5
27	5540.0
28	6863.0
29	8186.0
30	9761.0
31	11336.0
32	13106.0
33	14876.0
34	16952.5
35	19029.0
36	21073.5
37	23118.0
38	25838.0
39	28558.0
40	31056.0
41	33554.0
42	34747.0
43	35940.0
44	45584.5
45	55229.0
46	60047.5
47	64866.0
48	69413.0
49	73960.0
50	71332.0
51	68704.0
52	63421.5
53	58139.0
54	53283.0
55	48427.0
56	44804.0
57	41181.0
58	39684.5
59	38188.0
60	37262.5
61	36337.0
62	33257.0
63	30177.0
64	26425.5
65	22674.0
66	18789.0
67	14904.0
68	12587.5
69	10271.0
70	8910.0
71	7549.0
72	6431.5
73	5314.0
74	4549.0
75	3065.0
76	2346.0
77	1844.5
78	1343.0
79	1099.5
80	856.0
81	541.0
82	226.0
83	223.0
84	220.0
85	137.5
86	55.0
87	44.0
88	33.0
89	26.5
90	20.0
91	18.0
92	16.0
93	9.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	780645.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.823995257499444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.48112516282795	20.72362473303748
2	9.302092323943418	5.176427454124473
3	3.202224390507601	2.6729602876479768
4	1.6758585405908164	1.8651632034255528
5	1.0769603267321834	1.4982669511755664
6	0.8217117374561126	1.3717982091606304
7	0.6885768821681464	1.3411271932709183
8	0.5631222751158065	1.25346492097716
9	0.49860780589307707	1.2485935103869064
>10	6.875903971269882	44.166677160027476
>50	0.7438357725026306	12.850388271105373
>100	0.06395597295989325	3.3315534886637956
>500	0.0032441435559366146	0.5678960196654032
>1k	0.002780694476517098	1.9320585973312296
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCT	4202	0.5382728384861236	TruSeq Adapter, Index 3 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	3892	0.4985620864797699	TruSeq Adapter, Index 3 (95% over 24bp)
AATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGT	2381	0.3050041952488007	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCC	2370	0.3035951040485752	No Hit
AACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTG	1092	0.1398843264223815	RNA PCR Primer, Index 3 (95% over 22bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1046	0.1339917632214387	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	883	0.11311159361809785	RNA PCR Primer, Index 3 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2809920002049586E-4	0.0	0.0	0.5518513536882962	0.0
2	1.2809920002049586E-4	0.0	0.0	0.807537356929206	0.0
3	1.2809920002049586E-4	0.0	0.0	1.1644217281863074	0.0
4	1.2809920002049586E-4	0.0	0.0	2.5431534180069044	0.0
5	1.2809920002049586E-4	0.0	0.0	2.8265088484522414	0.0
6	1.2809920002049586E-4	0.0	0.0	3.8807652646209223	0.0
7	1.2809920002049586E-4	0.0	0.0	4.583773674333404	0.0
8	1.2809920002049586E-4	0.0	0.0	5.291137456846582	0.0
9	1.2809920002049586E-4	0.0	0.0	6.358203793017313	0.0
10	1.2809920002049586E-4	0.0	0.0	7.570278423611245	0.0
11	1.2809920002049586E-4	0.0	0.0	8.843840670215014	0.0
12	1.2809920002049586E-4	0.0	0.0	9.410423431905668	0.0
13	1.2809920002049586E-4	0.0	0.0	9.72862184475658	0.0
14	1.2809920002049586E-4	0.0	0.0	10.070006212811201	0.0
15	1.2809920002049586E-4	0.0	0.0	10.33068808485291	0.0
16	1.2809920002049586E-4	0.0	0.0	10.81669644973067	0.0
17	1.2809920002049586E-4	0.0	0.0	11.400572603424092	0.0
18	1.2809920002049586E-4	0.0	0.0	12.222200872355552	0.0
19	1.2809920002049586E-4	0.0	0.0	12.5599984628096	0.0
20	1.2809920002049586E-4	0.0	0.0	12.935457218069674	0.0
21	1.2809920002049586E-4	0.0	0.0	13.351267221336203	0.0
22	1.2809920002049586E-4	0.0	0.0	13.800511115808082	0.0
23	1.2809920002049586E-4	0.0	0.0	14.26538311268246	0.0
24	1.2809920002049586E-4	0.0	0.0	14.599081528735853	0.0
25	1.2809920002049586E-4	0.0	0.0	14.90575101358492	0.0
26	1.2809920002049586E-4	0.0	0.0	15.181036194428966	0.0
27	1.2809920002049586E-4	0.0	0.0	15.526519736884243	0.0
28	1.2809920002049586E-4	0.0	0.0	15.841899967334705	0.0
29	1.2809920002049586E-4	0.0	0.0	16.181106648988976	0.0
30	1.2809920002049586E-4	0.0	0.0	16.625610873060097	0.0
31	1.2809920002049586E-4	0.0	0.0	16.974040697115846	0.0
32	1.2809920002049586E-4	0.0	0.0	17.328106885972495	0.0
33	1.2809920002049586E-4	0.0	0.0	17.68242927322919	0.0
34	1.2809920002049586E-4	0.0	0.0	18.006648348481065	0.0
35	1.2809920002049586E-4	0.0	0.0	18.38351619494136	0.0
36	1.2809920002049586E-4	0.0	0.0	18.737326185397972	0.0
37	1.2809920002049586E-4	0.0	0.0	19.089983283054398	0.0
38	1.2809920002049586E-4	0.0	0.0	19.42086351670734	0.0
39	1.2809920002049586E-4	0.0	0.0	19.767756150362843	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	25	3.8896516E-5	45.0	1
CCGATAC	20	7.031894E-4	45.0	37
ACGATAC	20	7.031894E-4	45.0	11
CGTAGCG	40	6.8102963E-9	45.0	1
TCATACG	40	6.8102963E-9	45.0	1
CGGTATG	25	3.8896516E-5	45.0	1
CGACTCG	25	3.8896516E-5	45.0	1
CGATATG	20	7.031894E-4	45.0	1
CAATACG	25	3.8896516E-5	45.0	1
CTAGCGG	170	0.0	42.35294	2
TAGGGTA	135	0.0	41.666664	5
CGTAAAG	65	0.0	41.53846	1
AAACGCG	50	1.0804797E-9	40.5	1
CGATAGG	100	0.0	40.5	2
CATACGA	185	0.0	40.135136	18
CGCATAG	45	1.9268555E-8	40.0	1
TATCGCG	45	1.9268555E-8	40.0	1
ACGGGAT	740	0.0	39.527027	5
GTAACGG	80	0.0	39.375	2
CCGATCG	35	6.245713E-6	38.571426	26
>>END_MODULE