##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548526_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	475346
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.626434218442988	31.0	31.0	34.0	30.0	34.0
2	31.845352648386648	31.0	31.0	34.0	30.0	34.0
3	31.957666625994538	33.0	31.0	34.0	30.0	34.0
4	35.58281756867629	37.0	35.0	37.0	33.0	37.0
5	35.35204040846036	37.0	35.0	37.0	33.0	37.0
6	35.342699843903176	37.0	35.0	37.0	33.0	37.0
7	35.17931149099814	37.0	35.0	37.0	32.0	37.0
8	35.5764117085239	37.0	35.0	37.0	33.0	37.0
9	37.20491810176166	39.0	37.0	39.0	34.0	39.0
10	36.770794326658894	39.0	37.0	39.0	32.0	39.0
11	36.81180024655725	39.0	37.0	39.0	32.0	39.0
12	36.693936627214704	39.0	35.0	39.0	32.0	39.0
13	36.759568819344224	39.0	35.0	39.0	32.0	39.0
14	37.78977418554064	39.0	37.0	41.0	33.0	41.0
15	37.88691816066613	40.0	37.0	41.0	33.0	41.0
16	37.67661450816879	39.0	37.0	41.0	32.0	41.0
17	37.73896908778027	39.0	37.0	41.0	33.0	41.0
18	37.584376433166575	39.0	36.0	41.0	32.0	41.0
19	37.5510912051432	39.0	36.0	41.0	32.0	41.0
20	37.6150467238601	39.0	36.0	41.0	32.0	41.0
21	37.64199761857678	39.0	36.0	41.0	32.0	41.0
22	37.64570649589983	39.0	36.0	41.0	32.0	41.0
23	37.54946922873023	39.0	36.0	41.0	32.0	41.0
24	37.41842152873907	39.0	36.0	41.0	32.0	41.0
25	37.3726506586781	39.0	36.0	41.0	32.0	41.0
26	37.28124776478607	39.0	36.0	41.0	32.0	41.0
27	37.16974582725005	39.0	36.0	41.0	31.0	41.0
28	37.14222902895996	39.0	36.0	41.0	31.0	41.0
29	36.93561532020886	39.0	36.0	40.0	31.0	41.0
30	36.86949716627467	39.0	36.0	40.0	30.0	41.0
31	36.75067424570734	39.0	35.0	40.0	30.0	41.0
32	36.64476402452109	39.0	35.0	40.0	30.0	41.0
33	36.47163750194595	39.0	35.0	40.0	30.0	41.0
34	36.43708582800739	39.0	35.0	40.0	30.0	41.0
35	36.3531175186075	39.0	35.0	40.0	30.0	41.0
36	36.14262663407286	38.0	35.0	40.0	30.0	41.0
37	36.08828095744994	38.0	35.0	40.0	30.0	41.0
38	36.03168639264872	38.0	35.0	40.0	29.0	41.0
39	35.86009559352556	38.0	35.0	40.0	28.0	41.0
40	35.66643245130915	38.0	35.0	40.0	27.0	41.0
41	35.726079529437506	38.0	35.0	40.0	28.0	41.0
42	35.68033390414561	38.0	35.0	40.0	27.0	41.0
43	35.54566778725392	38.0	34.0	40.0	26.0	41.0
44	35.475504159075705	38.0	34.0	40.0	26.0	41.0
45	35.246698194578265	38.0	34.0	40.0	26.0	41.0
46	34.914786281992484	38.0	33.0	40.0	25.0	41.0
47	34.91403945757406	38.0	33.0	40.0	25.0	41.0
48	34.918482536930995	38.0	34.0	40.0	26.0	41.0
49	34.75766073554842	38.0	33.0	40.0	24.0	41.0
50	34.5786984638558	38.0	33.0	40.0	24.0	41.0
51	33.10263260866821	36.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	0.0
12	2.0
13	1.0
14	5.0
15	10.0
16	41.0
17	66.0
18	176.0
19	434.0
20	774.0
21	1244.0
22	1843.0
23	2241.0
24	2901.0
25	3349.0
26	4332.0
27	5265.0
28	6648.0
29	8492.0
30	11151.0
31	14036.0
32	17822.0
33	22697.0
34	29679.0
35	37706.0
36	46277.0
37	62834.0
38	91382.0
39	103894.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.071888687398232	38.8043656620651	18.96092530493577	24.16282034560089
2	20.10493409011541	37.34185204040846	19.471711132522415	23.081502736953713
3	18.82775914807319	29.84773196787182	27.933968099026814	23.39054078502817
4	24.899757229470744	29.435611112747345	18.66493038754928	26.999701270232627
5	16.6318849848321	39.079954391117205	18.979227762514043	25.308932861536647
6	20.175829816596753	33.46067916843731	24.24192062203111	22.12157039293483
7	75.51615034101475	10.612059426186399	7.490754103326839	6.381036129472005
8	78.08228111733348	4.313068796203186	5.347473208988821	12.257176877474512
9	74.68959452693407	8.335402001910188	10.427982985025643	6.547020486130103
10	28.362918800200276	40.200401391828265	13.820248829273835	17.616430978697622
11	20.517896437542337	26.670257033823784	30.619170036142094	22.192676492491785
12	22.208454473162707	23.977060919835235	35.81370201916078	18.000782587841275
13	25.51194287950251	26.894725105502097	25.888089938697284	21.705242076298106
14	17.874558742473905	28.247423981689128	33.31152465782819	20.566492618008777
15	16.16422563774598	30.145199496787605	28.03431605609388	25.656258809372538
16	18.123430090923243	29.525230042958185	29.017599811505722	23.33374005461285
17	17.332848072772254	27.520585005448662	29.642618219149842	25.50394870262924
18	17.441821325939422	27.441695102094055	28.775039655324754	26.341443916641772
19	18.353578235643088	30.920213907343282	31.29488835500877	19.431319502004854
20	19.925065110466903	32.142691849726305	27.869173191738227	20.063069848068565
21	18.036756383770978	28.402679311490996	28.853929558679365	24.70663474605866
22	17.789778393002152	29.627471357705755	28.18410168592983	24.398648563362265
23	19.75760814227952	27.03714767769162	25.701699393704796	27.50354478632407
24	15.302747893113647	35.10916258893522	25.82518838908921	23.762901128861923
25	19.499059632352015	27.757044342436878	26.647747114733267	26.09614891047784
26	22.030689224270322	30.26784699986957	24.58209388529618	23.119369890563927
27	16.525436208572284	28.44349168815978	28.833312997269356	26.197759105998582
28	23.352252885266733	26.547609530741816	26.22531797890379	23.874819605087662
29	19.509999032283854	29.65103314217433	28.7432733209073	22.09569450463452
30	22.671275239509747	29.176431483593003	24.866518283524	23.28577499337325
31	22.384536737450194	32.04255426573485	23.57356536081086	21.99934363600409
32	24.282943371775506	27.154535853883278	23.76731896344978	24.795201810891434
33	23.51024306505156	28.252893681655046	26.71170052971941	21.525162723573988
34	20.410185422828846	25.60387591354508	26.6517442031699	27.334194460456175
35	26.1998628367547	26.881050855587297	26.157788221632245	20.76129808602576
36	18.959031947255262	29.124258960841154	31.791999932680614	20.124709159222967
37	22.553466317166865	31.127431386821392	23.276518578046307	23.04258371796544
38	22.839994446150804	28.933240208185197	28.62588514471564	19.60088020094836
39	22.770150585047524	24.651727373323855	27.729275096456057	24.84884694517257
40	23.551686560947182	27.190509649812977	26.39256457401556	22.86523921522428
41	21.69261969176137	23.112006832917494	30.708368220201702	24.487005255119428
42	26.758613725580943	25.000946678840258	24.94099035229075	23.29944924328805
43	20.799585985787196	24.593243658303635	30.25270013842548	24.354470217483687
44	21.609732699970127	23.788145897935397	26.74620171411982	27.855919687974655
45	26.13633016791979	26.813941844466978	26.399506885510764	20.650221102102467
46	19.66020540827103	23.56872678007178	34.76330925262861	22.00775855902858
47	21.52095526206174	27.03062611234764	26.058702503018853	25.38971612257177
48	19.873734080017503	22.736070146798333	33.14175358580908	24.24844218737509
49	24.072359923087603	22.27956057271966	28.61473537170819	25.033344132484547
50	21.274398017444135	22.125777854447076	27.15579809233695	29.444026035771838
51	19.535664547508553	20.565440752630714	34.492979850466824	25.405914849393913
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2102.0
1	1658.0
2	1214.0
3	833.5
4	453.0
5	431.5
6	410.0
7	443.0
8	476.0
9	524.0
10	572.0
11	629.0
12	686.0
13	732.5
14	779.0
15	882.0
16	985.0
17	980.0
18	975.0
19	997.5
20	1020.0
21	1183.5
22	1347.0
23	1612.5
24	1878.0
25	2036.0
26	2742.5
27	3291.0
28	4500.5
29	5710.0
30	6473.0
31	7236.0
32	8420.0
33	9604.0
34	11417.0
35	13230.0
36	14700.5
37	16171.0
38	17142.5
39	18114.0
40	19780.5
41	21447.0
42	23864.0
43	26281.0
44	40510.0
45	54739.0
46	56487.5
47	58236.0
48	57703.0
49	57170.0
50	52144.0
51	47118.0
52	40923.0
53	34728.0
54	29171.5
55	23615.0
56	20320.5
57	17026.0
58	15463.0
59	13900.0
60	12006.5
61	10113.0
62	8923.5
63	7734.0
64	6597.0
65	5460.0
66	4278.5
67	3097.0
68	2661.5
69	2226.0
70	1872.5
71	1519.0
72	1226.0
73	933.0
74	702.0
75	434.0
76	397.0
77	316.0
78	235.0
79	205.5
80	176.0
81	168.0
82	160.0
83	120.5
84	81.0
85	48.5
86	16.0
87	14.5
88	13.0
89	9.0
90	5.0
91	2.5
92	0.0
93	1.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	475346.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.284022858953296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.26053203823662	23.544522046611814
2	11.031548800162065	6.902224496678578
3	4.118681936758609	3.865468195749431
4	2.0509767523425033	2.566512144138587
5	1.1562773984652035	1.8086504282438238
6	0.7539132909515974	1.415126437667909
7	0.5438658158487951	1.1910017430651894
8	0.3873930616381224	0.9695370716549541
9	0.3258389301679413	0.9174197285739687
>10	3.4827596075703475	25.091674219509592
>50	0.7499560767337227	16.060043229780433
>100	0.12813997713975478	6.394141674506047
>500	0.0033721046615724937	0.7682000210148656
>1k	0.004046525593886992	2.0997326583740574
>5k	0.002697683729257995	6.405745904430743
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AATGATACGGCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCC	9277	1.9516310224552222	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGT	8131	1.7105434778035369	No Hit
AATCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCT	7384	1.5533947903211556	No Hit
CTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGCT	5569	1.1715676580848478	No Hit
AATGACTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTT	2101	0.44199383186142305	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1750	0.368152882321509	No Hit
AATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTAAGTCGTC	1666	0.3504815439700765	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCT	1599	0.33638654790405303	No Hit
AACTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTG	1429	0.3006231250499636	No Hit
AATGATACGGCGACTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTAT	1407	0.2959949173864932	No Hit
AAACTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCT	954	0.20069591413412546	No Hit
CCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC	765	0.1609354028434025	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTT	748	0.15735906055799356	No Hit
AATGCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTC	606	0.12748608382104823	No Hit
AATGATCCGGCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCC	568	0.1194919069477812	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.3998224451241832	0.0
2	0.0	0.0	0.0	1.798900169560699	0.0
3	0.0	0.0	0.0	2.522162803515755	0.0
4	0.0	0.0	0.0	6.001523101067433	0.0
5	0.0	0.0	0.0	6.473810655817026	0.0
6	0.0	0.0	0.0	8.506645685458592	0.0
7	0.0	0.0	0.0	10.071190248787198	0.0
8	0.0	0.0	0.0	11.00524670450577	0.0
9	0.0	0.0	0.0	14.227110357508005	0.0
10	0.0	0.0	0.0	16.23764584113467	0.0
11	0.0	0.0	0.0	20.26923546216861	0.0
12	0.0	0.0	0.0	21.354339786176805	0.0
13	0.0	0.0	0.0	21.94590887479857	0.0
14	0.0	0.0	0.0	22.743643577520373	0.0
15	0.0	0.0	0.0	23.23823067828487	0.0
16	0.0	0.0	0.0	24.0774088769023	0.0
17	0.0	0.0	0.0	25.120438585788037	0.0
18	0.0	0.0	0.0	26.89493547857771	0.0
19	0.0	0.0	0.0	27.553823951395405	0.0
20	0.0	0.0	0.0	28.1870469089884	0.0
21	0.0	0.0	0.0	28.880436566206512	0.0
22	0.0	0.0	0.0	29.644932322981575	0.0
23	0.0	0.0	0.0	30.443087771854607	0.0
24	0.0	0.0	0.0	31.012988433688303	0.0
25	0.0	0.0	0.0	31.513676353645554	0.0
26	0.0	0.0	0.0	31.965347346985144	0.0
27	0.0	0.0	0.0	32.483496232218215	0.0
28	0.0	0.0	0.0	32.984815271402304	0.0
29	0.0	0.0	0.0	33.512641318113545	0.0
30	0.0	0.0	0.0	34.23948029435401	0.0
31	0.0	0.0	0.0	34.72901844130381	0.0
32	0.0	0.0	0.0	35.19562592301187	0.0
33	0.0	0.0	0.0	35.6445620663685	0.0
34	0.0	0.0	0.0	36.12484379799136	0.0
35	0.0	0.0	0.0	36.61248858726065	0.0
36	0.0	0.0	0.0	37.089404349673714	0.0
37	0.0	0.0	0.0	37.569265335145346	0.0
38	0.0	0.0	0.0	38.051440424448714	0.0
39	0.0	0.0	0.0	38.52583170995443	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATATGG	45	3.8380676E-10	45.000004	2
CCGGCGA	45	3.8380676E-10	45.000004	7
CGTAGGT	45	3.8380676E-10	45.000004	1
TCTAACG	45	3.8380676E-10	45.000004	1
GTAACGG	90	0.0	45.000004	2
CTTCGCC	25	3.887341E-5	45.0	36
CTAGACG	20	7.029108E-4	45.0	1
CGGGTCG	20	7.029108E-4	45.0	6
CGGGTAT	50	2.1827873E-11	45.0	6
ACACGTT	25	3.887341E-5	45.0	31
TACTTCG	35	1.209919E-7	45.0	13
CGAGTGG	20	7.029108E-4	45.0	28
ACGTTAG	20	7.029108E-4	45.0	1
CATGACG	40	6.8012014E-9	45.0	1
TAGAACG	20	7.029108E-4	45.0	40
AGCCAAT	25	3.887341E-5	45.0	42
GATCCGA	20	7.029108E-4	45.0	34
ATAGCGG	55	1.8189894E-12	45.0	2
GGATCGA	25	3.887341E-5	45.0	8
CACGATC	35	1.209919E-7	45.0	30
>>END_MODULE