SRR3548522_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548522_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	240120
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGCT	4727	1.968599033816425	No Hit
AAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	2398	0.9986673330001666	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT	2320	0.966183574879227	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2126	0.8853906380143263	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	1265	0.5268199233716475	No Hit
CCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	949	0.3952190571380976	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	671	0.2794436115275696	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	582	0.24237881059470265	No Hit
GAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT	471	0.19615192403798098	No Hit
CAAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	454	0.189072130601366	No Hit
AACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	439	0.18282525403964686	No Hit
AATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	436	0.18157587872730302	No Hit
ACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG	426	0.17741129435282357	No Hit
TAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT	410	0.1707479593536565	No Hit
GCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	362	0.15075795435615524	No Hit
TACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	338	0.14076295185740462	No Hit
CAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	332	0.138264201232717	No Hit
GAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	316	0.13160086623354988	No Hit
TGAGAGGGGTAAAGGCCCAAGGAAATTGTGTATCATAGAAGAAAACTCTTA	302	0.1257704481092787	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	301	0.12535398967183076	No Hit
TAAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	296	0.12327169748459103	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCC	293	0.1220223221722472	No Hit
CTCCCTGGGAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC	292	0.12160586373479926	No Hit
TCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	290	0.12077294685990338	No Hit
CTCCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCACTCACCATC	284	0.11827419623521573	No Hit
GAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	279	0.11619190404797601	No Hit
TCAAAAGGGGTGTAAACACTGTACCAAGTTTTGCAGATTCTTTCAAATCCA	279	0.11619190404797601	No Hit
AGCCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	266	0.11077794436115276	No Hit
AAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	256	0.10661335998667333	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGT	256	0.10661335998667333	No Hit
CACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	247	0.10286523404964185	No Hit
AACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG	241	0.10036648342495419	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGG	35	1.206572E-7	45.000004	2
TACCACG	35	1.206572E-7	45.000004	1
GCGAAGG	35	1.206572E-7	45.000004	2
TAAACGG	35	1.206572E-7	45.000004	2
GAACGGG	65	0.0	45.000004	3
ACCACGG	95	0.0	45.000004	2
ATTGGGT	35	1.206572E-7	45.000004	10
CTCGAGG	35	1.206572E-7	45.000004	2
GGGTTAC	35	1.206572E-7	45.000004	7
CCACAAA	20	7.022129E-4	45.0	31
TCGCCCA	25	3.8815604E-5	45.0	15
TATCAGG	45	3.8198777E-10	45.0	2
CGGGTGA	45	3.8198777E-10	45.0	6
CTAGACG	20	7.022129E-4	45.0	1
CGTGGGA	25	3.8815604E-5	45.0	4
AAGTACT	25	3.8815604E-5	45.0	24
AAGTACG	25	3.8815604E-5	45.0	1
GGTACTT	20	7.022129E-4	45.0	9
GATACCA	25	3.8815604E-5	45.0	9
GATACAA	20	7.022129E-4	45.0	33

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content