##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548522_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	240120
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56728302515409	31.0	31.0	34.0	30.0	34.0
2	31.781734132933533	31.0	31.0	34.0	30.0	34.0
3	31.95757537897718	33.0	31.0	34.0	30.0	34.0
4	35.628444111277695	37.0	35.0	37.0	33.0	37.0
5	35.39837164750958	37.0	35.0	37.0	33.0	37.0
6	35.39107529568549	37.0	35.0	37.0	33.0	37.0
7	35.325462268865564	37.0	35.0	37.0	33.0	37.0
8	35.575949525237384	37.0	35.0	37.0	35.0	37.0
9	37.160490588039316	39.0	37.0	39.0	34.0	39.0
10	36.80122855239047	39.0	37.0	39.0	32.0	39.0
11	36.89538564051308	39.0	37.0	39.0	33.0	39.0
12	36.92778610694653	39.0	37.0	39.0	33.0	39.0
13	36.8424121272697	39.0	37.0	39.0	33.0	39.0
14	37.921789105447274	40.0	37.0	41.0	33.0	41.0
15	37.905134932533734	40.0	37.0	41.0	33.0	41.0
16	37.790388139263705	39.0	37.0	41.0	33.0	41.0
17	37.80586373479927	39.0	37.0	41.0	33.0	41.0
18	37.72244294519407	39.0	37.0	41.0	33.0	41.0
19	37.64164167916042	39.0	36.0	41.0	32.0	41.0
20	37.53906380143262	39.0	36.0	41.0	32.0	41.0
21	37.4499833416625	39.0	36.0	40.0	32.0	41.0
22	37.45857487922705	39.0	36.0	40.0	32.0	41.0
23	37.30655089122106	39.0	36.0	40.0	32.0	41.0
24	37.22619523571547	39.0	36.0	41.0	32.0	41.0
25	37.18009328668999	39.0	36.0	41.0	32.0	41.0
26	37.13731884057971	39.0	36.0	40.0	32.0	41.0
27	37.1047976011994	39.0	36.0	40.0	31.0	41.0
28	36.964034649342	39.0	35.0	40.0	31.0	41.0
29	36.82520406463435	39.0	35.0	40.0	31.0	41.0
30	36.72829002165584	39.0	35.0	40.0	31.0	41.0
31	36.59453606530068	39.0	35.0	40.0	30.0	41.0
32	36.45715475595536	39.0	35.0	40.0	30.0	41.0
33	36.467220556388476	39.0	35.0	40.0	30.0	41.0
34	36.36625853739797	39.0	35.0	40.0	30.0	41.0
35	36.272597034815924	39.0	35.0	40.0	30.0	41.0
36	36.227802765284025	39.0	35.0	40.0	30.0	41.0
37	36.10908712310511	39.0	35.0	40.0	30.0	41.0
38	36.068320006663335	39.0	35.0	40.0	29.0	41.0
39	35.93975512243878	39.0	35.0	40.0	29.0	41.0
40	35.8416291854073	38.0	35.0	40.0	28.0	41.0
41	35.76283108445777	38.0	35.0	40.0	28.0	41.0
42	35.61386390138264	38.0	35.0	40.0	27.0	41.0
43	35.57902298850575	38.0	35.0	40.0	27.0	41.0
44	35.50359820089955	38.0	35.0	40.0	26.0	41.0
45	35.30747959353656	38.0	34.0	40.0	26.0	41.0
46	35.132941862402134	38.0	34.0	40.0	25.0	41.0
47	35.10642178910545	38.0	34.0	40.0	25.0	41.0
48	34.96930701316008	38.0	34.0	40.0	24.0	41.0
49	34.73163834749292	38.0	34.0	40.0	24.0	41.0
50	34.62925620523072	38.0	33.0	40.0	23.0	41.0
51	33.32104780942862	37.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	0.0
14	5.0
15	12.0
16	34.0
17	77.0
18	157.0
19	323.0
20	516.0
21	872.0
22	1232.0
23	1466.0
24	1775.0
25	1878.0
26	2201.0
27	2498.0
28	3068.0
29	3785.0
30	4906.0
31	6289.0
32	8061.0
33	10522.0
34	14644.0
35	19649.0
36	23208.0
37	32690.0
38	48849.0
39	51387.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.5992003998001	34.36906546726637	20.620523071797432	28.4112110611361
2	19.366150258204232	35.64134599366983	21.77494586040313	23.217557887722805
3	19.64767616191904	35.749625187406295	18.865150757954353	25.737547892720308
4	18.064301182741964	34.23205064134599	18.926786606696652	28.776861569215395
5	15.292770281525902	38.80851241046144	18.705647176411794	27.193070131600866
6	19.547309678494088	38.56863235049142	19.08837248042645	22.79568549058804
7	73.01765783774779	8.181326003664834	10.54056305180743	8.260453106779943
8	75.67133100116608	8.004747626186907	8.030984507746126	8.292936864900883
9	67.04939197068133	11.888222555388971	15.147426286856572	5.91495918707313
10	22.39130434782609	43.54281192736965	14.738047642845245	19.32783608195902
11	16.75537231384308	29.628519073796433	35.54514409461936	18.070964517741128
12	20.810011660836246	23.814759287023154	35.98450774612694	19.39072130601366
13	23.431617524571045	26.218973846410126	29.743878060969514	20.60553056804931
14	16.74121272696985	29.410294852573713	35.05455605530568	18.793936365150756
15	14.40904547726137	32.69823421622522	31.812427119773446	21.08029318673996
16	15.057054805930367	32.49500249875062	29.080876228552388	23.367066466766616
17	15.701316008662335	32.28427452940197	30.005413959686823	22.008995502248876
18	16.709978344161254	27.509162085623856	28.87056471764118	26.910294852573713
19	16.636265200732968	34.40029985007496	29.970847909378644	18.992587039813426
20	18.434116275195738	30.678827253040147	29.474845910378143	21.412210561385976
21	17.156005330668002	33.11302681992337	27.121022821922374	22.609945027486255
22	15.65550558054306	29.563135099117112	30.588872230551388	24.19248708978844
23	18.399966683325005	28.893053473263368	27.219723471597533	25.487256371814095
24	14.172913543228386	31.667916041979012	26.6191904047976	27.539980009995002
25	16.380976178577377	32.04647676161919	26.682908545727134	24.889638514076296
26	19.592287189738464	31.34057971014493	26.365983674829252	22.701149425287355
27	15.06663334999167	29.21414292853573	27.183908045977013	28.53531567549559
28	18.389555222388807	29.812593703148426	26.866566716641678	24.93128435782109
29	19.155005830418123	31.04489421955689	23.002665333999666	26.797434616025324
30	17.81984007996002	30.361069465267366	26.380976178577377	25.438114276195233
31	16.594202898550726	31.263951357654506	20.602615358987173	31.5392303848076
32	17.385473929701817	33.248375812093954	23.208812260536398	26.157337997667835
33	15.37481259370315	27.95977011494253	25.784607696151923	30.880809595202397
34	13.906380143261702	29.68182575378977	24.281609195402297	32.13018490754623
35	16.25312343828086	31.08070964517741	23.053889721805763	29.612277194735963
36	15.226136931534231	25.189905047476262	31.17649508579044	28.407462935199067
37	15.660503081792438	30.433533233383308	23.56071964017991	30.345244044644343
38	19.0184074629352	28.90429785107446	27.842745294019654	24.23454939197068
39	17.384641012826922	25.451857404631017	26.660003331667497	30.50349825087456
40	20.069548559053807	28.588205897051473	21.79743461602532	29.544810927869396
41	16.958604031317677	26.35640513076795	28.361652507079793	28.323338330834584
42	20.892470431450942	28.635265700483092	26.23563218390805	24.23663168415792
43	16.635015825420624	25.215309012160585	29.596035315675493	28.553639846743295
44	20.175745460603032	23.009328668998837	28.025570548059303	28.78935532233883
45	21.833250041645844	23.726886556721638	27.742378810594705	26.697484591037814
46	16.61294352823588	24.29576878227553	32.06854905880393	27.022738630684657
47	18.27461269365317	23.409128768948857	26.60169915042479	31.71455938697318
48	18.50991171081126	22.146426786606696	33.63193403298351	25.711727469598532
49	19.078377477927702	21.96818257537898	31.19023821422622	27.763201732467103
50	17.188072630351492	19.578544061302683	28.96343494919207	34.26994835915376
51	17.69990004997501	19.728052640346494	34.47442945194069	28.0976178577378
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2505.0
1	2035.0
2	1565.0
3	1099.0
4	633.0
5	560.0
6	487.0
7	510.0
8	533.0
9	559.5
10	586.0
11	639.0
12	692.0
13	751.5
14	811.0
15	871.5
16	932.0
17	1028.0
18	1124.0
19	1193.5
20	1263.0
21	1070.0
22	877.0
23	1215.0
24	1553.0
25	1656.5
26	1931.5
27	2103.0
28	2396.5
29	2690.0
30	3395.5
31	4101.0
32	4234.0
33	4367.0
34	4669.0
35	4971.0
36	6001.5
37	7032.0
38	7484.0
39	7936.0
40	8420.0
41	8904.0
42	12291.0
43	15678.0
44	19472.0
45	23266.0
46	25126.0
47	26986.0
48	30466.0
49	33946.0
50	29991.0
51	26036.0
52	21610.0
53	17184.0
54	14722.0
55	12260.0
56	9624.5
57	6989.0
58	6287.5
59	5586.0
60	5007.0
61	4428.0
62	3918.5
63	3409.0
64	3055.0
65	2701.0
66	2254.5
67	1808.0
68	1412.0
69	1016.0
70	758.0
71	500.0
72	402.5
73	305.0
74	261.0
75	219.5
76	222.0
77	157.5
78	93.0
79	63.0
80	33.0
81	19.5
82	6.0
83	8.5
84	11.0
85	9.0
86	7.0
87	4.0
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	2.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	240120.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.11377644511078
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.26796396220426	25.676744960852904
2	11.101887345264545	7.574546060303182
3	4.342359059501429	4.444027986006996
4	2.2584661962546084	3.081792437114776
5	1.2525331445174208	2.136431784107946
6	0.7764240544961789	1.5892053973013494
7	0.5151744512537539	1.2302182242212227
8	0.4004199526332495	1.0927869398634016
9	0.3308347779378372	1.0157421289355322
>10	2.971409038747955	24.122105613859738
>50	0.6775398588763826	15.30568049308679
>100	0.09522181800424835	6.4680159920039975
>500	0.0036623776155480137	0.9170414792603698
>1k	0.006103962692580022	5.345660503081792
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGCT	4727	1.968599033816425	No Hit
AAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	2398	0.9986673330001666	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT	2320	0.966183574879227	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2126	0.8853906380143263	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	1265	0.5268199233716475	No Hit
CCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	949	0.3952190571380976	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	671	0.2794436115275696	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	582	0.24237881059470265	No Hit
GAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT	471	0.19615192403798098	No Hit
CAAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	454	0.189072130601366	No Hit
AACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	439	0.18282525403964686	No Hit
AATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	436	0.18157587872730302	No Hit
ACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG	426	0.17741129435282357	No Hit
TAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT	410	0.1707479593536565	No Hit
GCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	362	0.15075795435615524	No Hit
TACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	338	0.14076295185740462	No Hit
CAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	332	0.138264201232717	No Hit
GAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	316	0.13160086623354988	No Hit
TGAGAGGGGTAAAGGCCCAAGGAAATTGTGTATCATAGAAGAAAACTCTTA	302	0.1257704481092787	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	301	0.12535398967183076	No Hit
TAAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	296	0.12327169748459103	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCC	293	0.1220223221722472	No Hit
CTCCCTGGGAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC	292	0.12160586373479926	No Hit
TCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	290	0.12077294685990338	No Hit
CTCCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCACTCACCATC	284	0.11827419623521573	No Hit
GAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	279	0.11619190404797601	No Hit
TCAAAAGGGGTGTAAACACTGTACCAAGTTTTGCAGATTCTTTCAAATCCA	279	0.11619190404797601	No Hit
AGCCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	266	0.11077794436115276	No Hit
AAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	256	0.10661335998667333	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGT	256	0.10661335998667333	No Hit
CACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	247	0.10286523404964185	No Hit
AACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG	241	0.10036648342495419	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.2184740962851905	0.0
2	0.0	0.0	0.0	3.0922038980509745	0.0
3	0.0	0.0	0.0	3.8988838913876394	0.0
4	0.0	0.0	0.0	7.913959686823255	0.0
5	0.0	0.0	0.0	8.590704647676162	0.0
6	0.0	0.0	0.0	12.104780942861902	0.0
7	0.0	0.0	0.0	14.99791770781276	0.0
8	0.0	0.0	0.0	16.812010661336	0.0
9	0.0	0.0	0.0	19.11544227886057	0.0
10	0.0	0.0	0.0	22.616608362485422	0.0
11	0.0	0.0	0.0	25.468099283691487	0.0
12	0.0	0.0	0.0	27.187656171914043	0.0
13	0.0	0.0	0.0	27.878977178077626	0.0
14	0.0	0.0	0.0	28.47326336831584	0.0
15	0.0	0.0	0.0	29.106696651674163	0.0
16	0.0	0.0	0.0	30.28735632183908	0.0
17	0.0	0.0	0.0	31.791604197901048	0.0
18	0.0	0.0	0.0	34.0896218557388	0.0
19	0.0	0.0	0.0	34.893803098450775	0.0
20	0.0	0.0	0.0	35.88997168082626	0.0
21	0.0	0.0	0.0	36.76411794102948	0.0
22	0.0	0.0	0.0	37.65950358154256	0.0
23	0.0	0.0	0.0	38.44494419456938	0.0
24	0.0	0.0	0.0	39.00049975012494	0.0
25	0.0	0.0	0.0	39.436531734132934	0.0
26	0.0	0.0	0.0	39.93295019157088	0.0
27	0.0	0.0	0.0	40.48350824587706	0.0
28	0.0	0.0	0.0	40.98992170581376	0.0
29	4.164584374479427E-4	0.0	0.0	41.542978510744625	0.0
30	4.164584374479427E-4	0.0	0.0	42.26178577377978	0.0
31	4.164584374479427E-4	0.0	0.0	42.77402965184075	0.0
32	4.164584374479427E-4	0.0	0.0	43.220056638347494	0.0
33	4.164584374479427E-4	0.0	0.0	43.65608862235549	0.0
34	4.164584374479427E-4	0.0	0.0	44.07712810261536	0.0
35	4.164584374479427E-4	0.0	0.0	44.496501749125436	0.0
36	4.164584374479427E-4	0.0	0.0	44.925453939696816	0.0
37	4.164584374479427E-4	0.0	0.0	45.355239047143094	0.0
38	4.164584374479427E-4	0.0	0.0	45.76086956521739	0.0
39	4.164584374479427E-4	0.0	0.0	46.15150757954356	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGG	35	1.206572E-7	45.000004	2
TACCACG	35	1.206572E-7	45.000004	1
GCGAAGG	35	1.206572E-7	45.000004	2
TAAACGG	35	1.206572E-7	45.000004	2
GAACGGG	65	0.0	45.000004	3
ACCACGG	95	0.0	45.000004	2
ATTGGGT	35	1.206572E-7	45.000004	10
CTCGAGG	35	1.206572E-7	45.000004	2
GGGTTAC	35	1.206572E-7	45.000004	7
CCACAAA	20	7.022129E-4	45.0	31
TCGCCCA	25	3.8815604E-5	45.0	15
TATCAGG	45	3.8198777E-10	45.0	2
CGGGTGA	45	3.8198777E-10	45.0	6
CTAGACG	20	7.022129E-4	45.0	1
CGTGGGA	25	3.8815604E-5	45.0	4
AAGTACT	25	3.8815604E-5	45.0	24
AAGTACG	25	3.8815604E-5	45.0	1
GGTACTT	20	7.022129E-4	45.0	9
GATACCA	25	3.8815604E-5	45.0	9
GATACAA	20	7.022129E-4	45.0	33
>>END_MODULE