FastQCFastQC Report
Sat 18 Jun 2016
SRR3548521_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548521_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240797
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGCT48852.028679759299327No Hit
AAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT24691.0253450001453506No Hit
AAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT22670.9414569118386026No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22590.9381346113116027No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT12400.5149565816849878No Hit
CCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC9050.3758352471168661No Hit
GAAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT6730.27948853183386835No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5530.22965402392886952No Hit
GAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT4780.19850745648824528No Hit
CAAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT4500.18687940464374556No Hit
ACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG4020.16694560148174603No Hit
AATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT3970.1648691636523711No Hit
AACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT3780.15697869990074628No Hit
TAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT3770.15656341233487128No Hit
GAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC3410.14161305996337165No Hit
GCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC3350.1391213345681217No Hit
CTCCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCACTCACCATC3340.1387060470022467No Hit
CAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT3340.1387060470022467No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCC3290.13662960917287176No Hit
GAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT3170.13164615838237187No Hit
TCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC3160.13123087081649687No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3070.12749328272362198No Hit
TGAGAGGGGTAAAGGCCCAAGGAAATTGTGTATCATAGAAGAAAACTCTTA3060.12707799515774698No Hit
CTCCCTGGGAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC2950.1225098319331221No Hit
TACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT2920.12126396923549712No Hit
TAAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT2830.11752638114262222No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGT2820.11711109357674722No Hit
AACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG2700.11212764278624733No Hit
TCAAAAGGGGTGTAAACACTGTACCAAGTTTTGCAGATTCTTTCAAATCCA2610.10839005469337243No Hit
AGCCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT2490.10340660390287255No Hit
CACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT2480.10299131633699754No Hit
AAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC2460.10216074120524758No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATA351.2065902E-745.00000431
GGCCCTA351.2065902E-745.00000440
CATAACG351.2065902E-745.0000041
ACGCGAG207.022168E-445.01
TATTTGC207.022168E-445.019
TCGCCAC207.022168E-445.016
GTGTTCG207.022168E-445.09
CGGGTTG453.8198777E-1045.06
GTGTTAC207.022168E-445.039
CGGGTTA207.022168E-445.06
CGTGGTA207.022168E-445.027
GAGGATA207.022168E-445.07
GAGTGTC253.8815924E-545.09
CGGGTGA253.8815924E-545.06
CTAGAAC207.022168E-445.020
CTAGAAA253.8815924E-545.043
CGTGGAC207.022168E-445.020
CGGCGGA253.8815924E-545.025
TCGTTAA207.022168E-445.017
ACCGGTA253.8815924E-545.010
Produced by FastQC (version 0.11.5)