##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548521_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	240797
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.543370556942154	31.0	31.0	34.0	30.0	34.0
2	31.74477256776455	31.0	31.0	34.0	30.0	34.0
3	31.927258229961335	33.0	31.0	34.0	30.0	34.0
4	35.572062774868456	37.0	35.0	37.0	33.0	37.0
5	35.369257092073404	37.0	35.0	37.0	33.0	37.0
6	35.374713970689	37.0	35.0	37.0	33.0	37.0
7	35.338392920177576	37.0	35.0	37.0	33.0	37.0
8	35.60730407770861	37.0	35.0	37.0	35.0	37.0
9	37.163955531007446	39.0	37.0	39.0	34.0	39.0
10	36.7933736715989	39.0	37.0	39.0	32.0	39.0
11	36.900127493282724	39.0	37.0	39.0	33.0	39.0
12	36.9419303396637	39.0	37.0	39.0	33.0	39.0
13	36.838473901252925	39.0	37.0	39.0	33.0	39.0
14	37.90854537224301	40.0	37.0	41.0	33.0	41.0
15	37.88727849599455	39.0	37.0	41.0	33.0	41.0
16	37.81122688405586	39.0	37.0	41.0	33.0	41.0
17	37.79587370274547	39.0	37.0	41.0	33.0	41.0
18	37.70035756259422	39.0	37.0	41.0	33.0	41.0
19	37.5969758759453	39.0	36.0	41.0	32.0	41.0
20	37.496737916170055	39.0	36.0	41.0	32.0	41.0
21	37.42412488527681	39.0	36.0	40.0	32.0	41.0
22	37.42565314351923	39.0	36.0	40.0	32.0	41.0
23	37.29151941261727	39.0	36.0	40.0	32.0	41.0
24	37.23288080831572	39.0	36.0	40.0	32.0	41.0
25	37.18839105138353	39.0	36.0	41.0	32.0	41.0
26	37.114291291004456	39.0	36.0	40.0	31.0	41.0
27	37.054523104523724	39.0	36.0	40.0	31.0	41.0
28	36.906556144802465	39.0	35.0	40.0	31.0	41.0
29	36.77979792107045	39.0	35.0	40.0	31.0	41.0
30	36.722712492265266	39.0	35.0	40.0	31.0	41.0
31	36.58814270941914	39.0	35.0	40.0	30.0	41.0
32	36.45566182302936	39.0	35.0	40.0	30.0	41.0
33	36.450620231979634	39.0	35.0	40.0	30.0	41.0
34	36.34832659875331	39.0	35.0	40.0	30.0	41.0
35	36.249974044527136	39.0	35.0	40.0	30.0	41.0
36	36.210642159163115	39.0	35.0	40.0	30.0	41.0
37	36.04619658882793	39.0	35.0	40.0	29.0	41.0
38	35.989119465774074	39.0	35.0	40.0	29.0	41.0
39	35.88654758987861	38.0	35.0	40.0	29.0	41.0
40	35.81394701761234	38.0	35.0	40.0	28.0	41.0
41	35.78777144233524	38.0	35.0	40.0	28.0	41.0
42	35.607424511102714	38.0	35.0	40.0	27.0	41.0
43	35.563794399431885	38.0	35.0	40.0	27.0	41.0
44	35.562237071059855	38.0	35.0	40.0	27.0	41.0
45	35.368571867589715	38.0	34.0	40.0	26.0	41.0
46	35.16287993621183	38.0	34.0	40.0	25.0	41.0
47	35.0844362678937	38.0	34.0	40.0	25.0	41.0
48	34.9901742961914	38.0	34.0	40.0	24.0	41.0
49	34.78701977184101	38.0	34.0	40.0	24.0	41.0
50	34.64143656274788	38.0	33.0	40.0	23.0	41.0
51	33.3730735848038	37.0	31.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	1.0
14	5.0
15	6.0
16	33.0
17	69.0
18	165.0
19	336.0
20	557.0
21	872.0
22	1189.0
23	1529.0
24	1783.0
25	1923.0
26	2105.0
27	2498.0
28	3108.0
29	3853.0
30	4932.0
31	6366.0
32	8124.0
33	10641.0
34	14602.0
35	19882.0
36	23253.0
37	32696.0
38	49124.0
39	51132.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.475288313392607	34.446027151501056	20.57334601344701	28.505338521659322
2	19.256053854491544	35.8285194582989	21.827099174823605	23.088327512385952
3	19.738202718472404	35.78283782605265	18.743588998201805	25.735370457273138
4	18.32041096857519	34.06562374115957	18.83412168756255	28.77984360270269
5	15.15052097825139	38.847660062210075	18.615680427912306	27.386138531626226
6	19.540110549550036	38.630464665257456	18.96535255837905	22.864072226813455
7	73.07939882971965	8.19819183793818	10.455695046034627	8.266714286307554
8	75.80783813751833	7.989717479868935	8.037475549944558	8.16496883266818
9	67.32019086616528	11.817007686972843	15.085320830409016	5.777480616452863
10	22.49737330614584	43.56325037272059	14.54627756990328	19.39309875123029
11	16.687915547120603	29.499536954364046	35.71306951498565	18.099477983529695
12	20.739461039797007	23.88941722695881	36.06980153407227	19.30132019917192
13	23.428863316403444	25.992848748115634	29.942648787152663	20.63563914832826
14	16.687084971988853	29.26489947964468	35.03615078260942	19.011864765757046
15	14.385976569475535	32.41817796733348	32.07639630061836	21.11944916257262
16	14.803340573179899	32.590937594737476	29.08341881335731	23.522303018725317
17	15.606506725582129	32.227976262162734	30.00120433394104	22.1643126783141
18	16.805026640697353	27.41396279853985	28.70675299110869	27.074257569654108
19	16.70909521298023	34.6079893021923	29.800620439623415	18.88229504520405
20	18.49773875920381	30.544400470105526	29.267391205039928	21.690469565650737
21	17.03883354028497	33.27408564060184	27.2187776425786	22.46830317653459
22	15.54670531609613	29.30975053675918	30.55229093385715	24.59125321328754
23	18.344912934961815	29.023202116305434	27.23995730843823	25.39192764029452
24	13.956153938794918	31.570991332948502	26.758639019589115	27.714215708667467
25	16.429191393580485	32.10795815562486	26.537290746977742	24.92555970381691
26	19.572502979688284	31.111683285090763	26.537706034543618	22.77810770067733
27	15.20700008721039	29.303105935705183	27.04228042708173	28.4476135500027
28	18.195824698812693	29.5485408871373	27.227083393896102	25.028551020153905
29	19.21826268599692	31.089673044099385	22.86531808951108	26.826746180392615
30	18.007284143905448	30.33924841256328	26.345012603977626	25.30845483955365
31	16.755192132792352	31.283197049797135	20.398094660647764	31.56351615676275
32	17.366495429760338	33.217606531642836	23.296386582889323	26.119511455707507
33	15.391387766458884	27.895696374954838	25.82050440827751	30.892411450308764
34	14.051254791380291	29.699290273549924	24.262760748680424	31.986694186389364
35	16.35693135711824	31.043576124287263	22.933840537880457	29.665651980714046
36	15.266801496696386	25.349983596141147	31.138676976872638	28.24453793028983
37	15.670876298292752	30.32346748506003	23.46831563516157	30.53734058148565
38	19.0043895895713	29.011158776895062	27.88323774797859	24.101213885555055
39	17.316245634289466	25.40770856779777	26.531476721055498	30.74456907685727
40	20.0720939214359	28.612898001220945	21.79429145711948	29.520716620223673
41	16.97986270593072	26.301407409560753	28.309738078132202	28.408991806376328
42	20.965377475633	28.694709651698318	26.137784108606006	24.202128764062675
43	16.440819445424985	25.33295680594027	29.659837954791797	28.566385793842947
44	20.22201273271677	23.068808996789826	28.00408642964821	28.705091840845192
45	21.974941548275105	23.54016038405794	27.96421882332421	26.520679244342745
46	16.711171650809604	24.42057002371292	31.78652557963762	27.081732745839858
47	18.203299874998443	23.57089166393269	26.65938529134499	31.566423169723873
48	18.580380984812933	22.233665701815223	33.40780823681358	25.77814507655826
49	18.986116936672797	22.153099914035472	31.060187626922264	27.800595522369463
50	17.041740553246097	19.605310697392408	28.928931838851813	34.42401691050968
51	17.69789490732858	19.81336976789578	34.53282225276893	27.95591307200671
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2654.0
1	2103.0
2	1552.0
3	1090.0
4	628.0
5	558.5
6	489.0
7	506.5
8	524.0
9	559.0
10	594.0
11	657.5
12	721.0
13	748.0
14	775.0
15	843.0
16	911.0
17	960.5
18	1010.0
19	1108.0
20	1206.0
21	1042.0
22	878.0
23	1154.0
24	1430.0
25	1556.5
26	1902.0
27	2121.0
28	2442.5
29	2764.0
30	3450.0
31	4136.0
32	4259.0
33	4382.0
34	4661.0
35	4940.0
36	5948.5
37	6957.0
38	7535.0
39	8113.0
40	8453.5
41	8794.0
42	12243.0
43	15692.0
44	19617.0
45	23542.0
46	25312.5
47	27083.0
48	30629.0
49	34175.0
50	30063.5
51	25952.0
52	21608.0
53	17264.0
54	14760.5
55	12257.0
56	9636.5
57	7016.0
58	6258.0
59	5500.0
60	5043.0
61	4586.0
62	3981.5
63	3377.0
64	3091.0
65	2805.0
66	2350.0
67	1895.0
68	1435.5
69	976.0
70	782.5
71	589.0
72	427.5
73	266.0
74	233.0
75	211.0
76	222.0
77	154.5
78	87.0
79	54.5
80	22.0
81	12.5
82	3.0
83	9.0
84	15.0
85	9.0
86	3.0
87	2.0
88	1.0
89	1.5
90	2.0
91	2.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	240797.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.015373945688694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.24783903892171	25.595833835139143
2	11.066074131952924	7.528332994181822
3	4.354885969624457	4.443992242428269
4	2.207354592957953	3.0033596764079284
5	1.35639986326122	2.3069224284355703
6	0.7801435757190994	1.592212527564712
7	0.5164330712506715	1.2296664825558457
8	0.42364604190066907	1.1528382828689727
9	0.31376666503882406	0.9605601398688521
>10	2.913024368804024	23.38110524632782
>50	0.709332421741466	15.873951918005623
>100	0.10133320310592372	6.597673559055968
>500	0.003662645895394833	0.8849778028796039
>1k	0.006104409825658056	5.44857286427987
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGCT	4885	2.028679759299327	No Hit
AAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	2469	1.0253450001453506	No Hit
AAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT	2267	0.9414569118386026	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2259	0.9381346113116027	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	1240	0.5149565816849878	No Hit
CCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	905	0.3758352471168661	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	673	0.27948853183386835	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	553	0.22965402392886952	No Hit
GAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT	478	0.19850745648824528	No Hit
CAAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	450	0.18687940464374556	No Hit
ACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG	402	0.16694560148174603	No Hit
AATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	397	0.1648691636523711	No Hit
AACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	378	0.15697869990074628	No Hit
TAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTT	377	0.15656341233487128	No Hit
GAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	341	0.14161305996337165	No Hit
GCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	335	0.1391213345681217	No Hit
CTCCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCACTCACCATC	334	0.1387060470022467	No Hit
CAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	334	0.1387060470022467	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCC	329	0.13662960917287176	No Hit
GAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	317	0.13164615838237187	No Hit
TCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	316	0.13123087081649687	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	307	0.12749328272362198	No Hit
TGAGAGGGGTAAAGGCCCAAGGAAATTGTGTATCATAGAAGAAAACTCTTA	306	0.12707799515774698	No Hit
CTCCCTGGGAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC	295	0.1225098319331221	No Hit
TACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	292	0.12126396923549712	No Hit
TAAAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCT	283	0.11752638114262222	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGT	282	0.11711109357674722	No Hit
AACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTG	270	0.11212764278624733	No Hit
TCAAAAGGGGTGTAAACACTGTACCAAGTTTTGCAGATTCTTTCAAATCCA	261	0.10839005469337243	No Hit
AGCCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	249	0.10340660390287255	No Hit
CACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCT	248	0.10299131633699754	No Hit
AAAACTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	246	0.10216074120524758	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.2541809075694466	0.0
2	0.0	0.0	0.0	3.0934770782028016	0.0
3	0.0	0.0	0.0	3.8912444922486578	0.0
4	0.0	0.0	0.0	7.843536256680939	0.0
5	0.0	0.0	0.0	8.505919924251549	0.0
6	0.0	0.0	0.0	12.026727907739714	0.0
7	0.0	0.0	0.0	14.938724319655146	0.0
8	0.0	0.0	0.0	16.778032948915477	0.0
9	0.0	0.0	0.0	18.9545550816663	0.0
10	0.0	0.0	0.0	22.482007666208467	0.0
11	0.0	0.0	0.0	25.327142780018022	0.0
12	0.0	0.0	0.0	27.113294600846356	0.0
13	0.0	0.0	0.0	27.787306320261465	0.0
14	0.0	0.0	0.0	28.400686055058827	0.0
15	0.0	0.0	0.0	29.045627644862684	0.0
16	0.0	0.0	0.0	30.22296789411828	0.0
17	0.0	0.0	0.0	31.666922760665624	0.0
18	0.0	0.0	0.0	34.00499175654182	0.0
19	0.0	0.0	0.0	34.8272611369743	0.0
20	0.0	0.0	0.0	35.848037973895025	0.0
21	0.0	0.0	0.0	36.700208059070505	0.0
22	0.0	0.0	0.0	37.60221265215098	0.0
23	0.0	0.0	0.0	38.41991386935884	0.0
24	0.0	0.0	0.0	38.97764507032895	0.0
25	0.0	0.0	0.0	39.46270094727094	0.0
26	0.0	0.0	0.0	39.97018235277017	0.0
27	0.0	0.0	0.0	40.53497344236016	0.0
28	0.0	0.0	0.0	41.0457771483864	0.0
29	0.0	0.0	0.0	41.57983695810164	0.0
30	0.0	0.0	0.0	42.26340029153187	0.0
31	0.0	0.0	0.0	42.73184466583886	0.0
32	0.0	0.0	0.0	43.17038833540285	0.0
33	0.0	0.0	0.0	43.606855567137465	0.0
34	0.0	0.0	0.0	44.028787734066455	0.0
35	0.0	0.0	0.0	44.44739760046844	0.0
36	0.0	0.0	0.0	44.86476160417281	0.0
37	0.0	0.0	0.0	45.27049755603267	0.0
38	0.0	0.0	0.0	45.69741317375217	0.0
39	0.0	0.0	0.0	46.078647159225405	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCATA	35	1.2065902E-7	45.000004	31
GGCCCTA	35	1.2065902E-7	45.000004	40
CATAACG	35	1.2065902E-7	45.000004	1
ACGCGAG	20	7.022168E-4	45.0	1
TATTTGC	20	7.022168E-4	45.0	19
TCGCCAC	20	7.022168E-4	45.0	16
GTGTTCG	20	7.022168E-4	45.0	9
CGGGTTG	45	3.8198777E-10	45.0	6
GTGTTAC	20	7.022168E-4	45.0	39
CGGGTTA	20	7.022168E-4	45.0	6
CGTGGTA	20	7.022168E-4	45.0	27
GAGGATA	20	7.022168E-4	45.0	7
GAGTGTC	25	3.8815924E-5	45.0	9
CGGGTGA	25	3.8815924E-5	45.0	6
CTAGAAC	20	7.022168E-4	45.0	20
CTAGAAA	25	3.8815924E-5	45.0	43
CGTGGAC	20	7.022168E-4	45.0	20
CGGCGGA	25	3.8815924E-5	45.0	25
TCGTTAA	20	7.022168E-4	45.0	17
ACCGGTA	25	3.8815924E-5	45.0	10
>>END_MODULE