FastQCFastQC Report
Sat 18 Jun 2016
SRR3548519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684553
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT48660.7108288182215255TruSeq Adapter, Index 21 (95% over 23bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG11030.1611270420259644No Hit
AAACTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCT10290.15031706821823876No Hit
AATCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCT10210.14914842240118734No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10120.1478336958570045No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCC10000.14608072713142736No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGT9040.13205697732681035No Hit
CCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC8880.1297196856927075TruSeq Adapter, Index 14 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTT7610.11116743334701623No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACG302.16421E-645.0000041
CTAAGCG302.16421E-645.0000041
TCTAGCG302.16421E-645.0000041
ATACACG600.045.0000041
TAATACG207.031285E-445.01
ATCTCGT207.031285E-445.019
CGTATTA207.031285E-445.031
TCCGCGA253.8891463E-545.033
GTATTCG253.8891463E-545.01
CGTATAG207.031285E-445.01
CGAAATC207.031285E-445.016
CGACGGT253.8891463E-545.028
GTGATCG207.031285E-445.027
CGCGAAT253.8891463E-545.035
TGACGGA253.8891463E-545.015
CATTCCG207.031285E-445.01
ATCCGTG207.031285E-445.015
TTATACG253.8891463E-545.01
ATTCCGG406.8084773E-945.02
ACGATTG207.031285E-445.01