Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548519_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 684553 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT | 4866 | 0.7108288182215255 | TruSeq Adapter, Index 21 (95% over 23bp) |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 1103 | 0.1611270420259644 | No Hit |
AAACTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCT | 1029 | 0.15031706821823876 | No Hit |
AATCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCT | 1021 | 0.14914842240118734 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1012 | 0.1478336958570045 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCC | 1000 | 0.14608072713142736 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGT | 904 | 0.13205697732681035 | No Hit |
CCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC | 888 | 0.1297196856927075 | TruSeq Adapter, Index 14 (95% over 21bp) |
AAAAACTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTT | 761 | 0.11116743334701623 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACCGACG | 30 | 2.16421E-6 | 45.000004 | 1 |
CTAAGCG | 30 | 2.16421E-6 | 45.000004 | 1 |
TCTAGCG | 30 | 2.16421E-6 | 45.000004 | 1 |
ATACACG | 60 | 0.0 | 45.000004 | 1 |
TAATACG | 20 | 7.031285E-4 | 45.0 | 1 |
ATCTCGT | 20 | 7.031285E-4 | 45.0 | 19 |
CGTATTA | 20 | 7.031285E-4 | 45.0 | 31 |
TCCGCGA | 25 | 3.8891463E-5 | 45.0 | 33 |
GTATTCG | 25 | 3.8891463E-5 | 45.0 | 1 |
CGTATAG | 20 | 7.031285E-4 | 45.0 | 1 |
CGAAATC | 20 | 7.031285E-4 | 45.0 | 16 |
CGACGGT | 25 | 3.8891463E-5 | 45.0 | 28 |
GTGATCG | 20 | 7.031285E-4 | 45.0 | 27 |
CGCGAAT | 25 | 3.8891463E-5 | 45.0 | 35 |
TGACGGA | 25 | 3.8891463E-5 | 45.0 | 15 |
CATTCCG | 20 | 7.031285E-4 | 45.0 | 1 |
ATCCGTG | 20 | 7.031285E-4 | 45.0 | 15 |
TTATACG | 25 | 3.8891463E-5 | 45.0 | 1 |
ATTCCGG | 40 | 6.8084773E-9 | 45.0 | 2 |
ACGATTG | 20 | 7.031285E-4 | 45.0 | 1 |