##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548519_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	684553
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.851063394653153	33.0	31.0	34.0	30.0	34.0
2	32.048666794243836	34.0	31.0	34.0	30.0	34.0
3	32.19335683285297	34.0	31.0	34.0	30.0	34.0
4	35.784044478659794	37.0	35.0	37.0	35.0	37.0
5	35.6118328310591	37.0	35.0	37.0	33.0	37.0
6	35.62145955097706	37.0	35.0	37.0	33.0	37.0
7	35.72497819745148	37.0	35.0	37.0	35.0	37.0
8	35.89201858731172	37.0	35.0	37.0	35.0	37.0
9	37.48866340517097	39.0	37.0	39.0	35.0	39.0
10	37.02932424516436	39.0	37.0	39.0	33.0	39.0
11	37.020993261296056	39.0	37.0	39.0	33.0	39.0
12	36.92708964828143	39.0	37.0	39.0	33.0	39.0
13	36.882613910098996	39.0	37.0	39.0	33.0	39.0
14	38.04860544033844	40.0	37.0	41.0	33.0	41.0
15	38.05229251789124	40.0	37.0	41.0	33.0	41.0
16	38.00302533185889	40.0	37.0	41.0	33.0	41.0
17	37.9797371423396	40.0	37.0	41.0	33.0	41.0
18	37.95320157825618	40.0	37.0	41.0	33.0	41.0
19	37.9419665095325	40.0	37.0	41.0	33.0	41.0
20	37.88074845921353	40.0	37.0	41.0	33.0	41.0
21	37.85091731392602	40.0	37.0	41.0	33.0	41.0
22	37.81757000553646	40.0	37.0	41.0	33.0	41.0
23	37.72261607209376	40.0	37.0	41.0	33.0	41.0
24	37.626145820703435	39.0	36.0	41.0	32.0	41.0
25	37.643548417726606	39.0	36.0	41.0	33.0	41.0
26	37.54409081546644	39.0	36.0	41.0	32.0	41.0
27	37.482273834166236	39.0	36.0	41.0	32.0	41.0
28	37.4493837584526	39.0	36.0	41.0	32.0	41.0
29	37.29262014774605	39.0	36.0	41.0	32.0	41.0
30	37.264064287206395	39.0	36.0	41.0	32.0	41.0
31	37.169829070941184	39.0	36.0	41.0	31.0	41.0
32	37.15550001241686	39.0	36.0	41.0	31.0	41.0
33	37.11460617366369	39.0	36.0	41.0	31.0	41.0
34	37.0052705926349	39.0	35.0	41.0	31.0	41.0
35	36.97703757050221	39.0	35.0	41.0	31.0	41.0
36	36.89948769488995	39.0	35.0	41.0	31.0	41.0
37	36.800504854992965	39.0	35.0	41.0	31.0	41.0
38	36.73214783953909	39.0	35.0	41.0	31.0	41.0
39	36.650120589640245	39.0	35.0	40.0	30.0	41.0
40	36.5311991912971	39.0	35.0	40.0	30.0	41.0
41	36.50059381815579	39.0	35.0	40.0	30.0	41.0
42	36.43763886799123	39.0	35.0	40.0	30.0	41.0
43	36.37425297968163	39.0	35.0	40.0	30.0	41.0
44	36.32442338284983	38.0	35.0	40.0	30.0	41.0
45	36.12497936609729	38.0	35.0	40.0	30.0	41.0
46	35.93699246077367	38.0	35.0	40.0	29.0	41.0
47	35.82701120293096	38.0	35.0	40.0	29.0	41.0
48	35.79927339446325	38.0	35.0	40.0	29.0	41.0
49	35.66002194132521	38.0	34.0	40.0	29.0	41.0
50	35.474160510581356	38.0	34.0	40.0	28.0	41.0
51	34.267340877915956	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	4.0
10	5.0
11	6.0
12	15.0
13	19.0
14	30.0
15	22.0
16	61.0
17	108.0
18	215.0
19	355.0
20	647.0
21	1061.0
22	1523.0
23	2058.0
24	2647.0
25	3476.0
26	4490.0
27	5645.0
28	7355.0
29	9524.0
30	12440.0
31	16147.0
32	21004.0
33	28249.0
34	43243.0
35	52638.0
36	63542.0
37	91452.0
38	141035.0
39	175476.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.545592525341352	31.921998734940903	19.522374454571086	27.010034285146656
2	25.187969375636364	29.128935232188013	19.865225921148543	25.817869471027077
3	22.420177838677212	30.173558511904847	20.33224600578772	27.07401764363022
4	22.401041263422993	31.205326687634123	19.181568118173466	27.21206393076942
5	19.09333535898608	33.67949596305911	17.192094695370557	30.035073982584255
6	21.610014126006313	37.00224818239055	19.169297337094427	22.218440354508708
7	83.79584926221929	4.840384893499846	6.283370316104085	5.080395528176781
8	84.96171954545521	4.331585720901084	5.266794535996482	5.439900197647224
9	76.62766798188014	8.489919699424297	9.580704488914664	5.301707829780893
10	33.045797768762974	34.6383698559498	13.90849211091033	18.407340264376902
11	26.737885890500806	28.05509580704489	26.601300410632923	18.605717891821378
12	27.576389264235203	23.94701359865489	29.453818769328304	19.022778367781605
13	26.63168520187626	24.646886362341558	26.825388246052533	21.89604018972965
14	20.89129694851969	28.191827367639906	28.83487472847245	22.082000955367956
15	20.956156791366045	28.01229415399538	28.712751240590574	22.318797814048
16	22.173739652006493	26.854312230024558	27.6354058779963	23.336542239972655
17	21.340203022994565	27.45572658362464	28.59647098179396	22.607599411586833
18	23.348666940324563	25.002885094360845	27.58968261040416	24.05876535491043
19	22.297031785705418	29.25967748297064	27.270057979440598	21.173232751883344
20	25.019100055072435	26.72269349487914	25.603861205779538	22.65434524426889
21	24.211711876217034	28.19255777127556	25.66755240280884	21.92817794969856
22	21.854699343951452	26.92107112232362	25.71371391258237	25.510515621142556
23	22.43434766920896	28.645700186837246	24.878424314844867	24.041527829108922
24	20.59825900989405	28.415476960878124	27.01047252732805	23.975791501899778
25	22.735712209281093	27.285104294335135	23.91619056523016	26.062992931153616
26	24.17636034025123	26.86395355801523	25.72116402966607	23.238522072067465
27	21.20946077221194	25.502919423331722	26.988560418258338	26.299059386198
28	21.261027268889336	26.307678149098756	28.13003522006331	24.3012593619486
29	23.033862973356335	27.936478256614173	25.614232937405866	23.415425832623622
30	24.605545516563364	24.786831698933465	26.625841972791005	23.98178081171217
31	23.76397444755921	26.955546173926635	24.62468209181758	24.655797286696572
32	24.36860257715619	25.240558437403678	26.47274937075727	23.918089614682867
33	22.32916954567433	25.163573894205417	27.61232512310953	24.89493143701072
34	20.88092521689336	25.628694929391884	28.239011442503358	25.251368411211406
35	23.45369898313206	23.294909232740196	28.467481699736908	24.783910084390836
36	22.842205059359905	27.43293799019214	29.27501595931944	20.449840991128518
37	22.885298873863675	27.486987859230766	26.098782709300817	23.528930557604745
38	24.5183353224659	25.363266248194076	27.193657759150863	22.92474067018916
39	24.133120445020328	24.266930391072712	25.356984776927426	26.242964386979533
40	24.71671294991038	25.241581002493596	25.799755460862784	24.24195058673324
41	21.666693448133305	24.49569280976053	28.620720382497776	25.216893359608388
42	23.1949900153823	24.722410098268504	26.39868644210163	25.68391344424756
43	21.83380979997166	23.91224638559761	28.91580345130326	25.338140363127472
44	23.724386570506592	23.05898885842294	27.42972421419525	25.786900356875215
45	25.613064291588817	22.675234788248684	28.4172299296037	23.294470990558803
46	21.886691023193237	24.58071179295102	29.48917030529411	24.04342687856163
47	22.71365401948425	25.330252003862373	26.926622189954614	25.029471786698764
48	22.176223024367726	24.27248145870371	29.431760579531463	24.119534937397106
49	22.831102924097916	23.55420252339848	28.458716856109024	25.155977696394583
50	21.82256158398254	23.356701380316792	27.317095973576915	27.50364106212375
51	21.884499812286265	21.889466557008735	30.56257148825584	25.663462142449163
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1284.0
1	1078.0
2	872.0
3	600.0
4	328.0
5	317.5
6	307.0
7	340.5
8	374.0
9	422.0
10	470.0
11	510.5
12	551.0
13	629.0
14	707.0
15	770.0
16	833.0
17	938.5
18	1044.0
19	1159.0
20	1274.0
21	1442.5
22	1611.0
23	2424.0
24	3237.0
25	3512.0
26	4619.0
27	5451.0
28	6513.0
29	7575.0
30	8566.5
31	9558.0
32	11072.5
33	12587.0
34	14317.0
35	16047.0
36	17801.0
37	19555.0
38	20951.0
39	22347.0
40	24338.0
41	26329.0
42	30530.0
43	34731.0
44	40035.0
45	45339.0
46	52512.0
47	59685.0
48	66310.5
49	72936.0
50	69311.5
51	65687.0
52	58929.0
53	52171.0
54	47634.5
55	43098.0
56	39302.5
57	35507.0
58	34482.5
59	33458.0
60	30317.0
61	27176.0
62	24504.0
63	21832.0
64	19646.5
65	17461.0
66	15426.5
67	13392.0
68	11001.5
69	8611.0
70	7223.0
71	5835.0
72	5035.0
73	4235.0
74	3691.5
75	2464.5
76	1781.0
77	1578.0
78	1375.0
79	913.0
80	451.0
81	316.0
82	181.0
83	155.0
84	129.0
85	121.0
86	113.0
87	75.5
88	38.0
89	31.5
90	25.0
91	18.5
92	12.0
93	10.5
94	9.0
95	7.5
96	6.0
97	4.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	684553.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.732917069325396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.68034015591203	19.696904230276818
2	10.776114323718316	5.7615394109106255
3	4.081723488055531	3.273491265183183
4	2.006733024632364	2.14583310111094
5	1.19079442529965	1.591670430907527
6	0.75001389238997	1.2030035511661805
7	0.5247464282572784	0.9819601924318572
8	0.44283609009248337	0.9470640377397335
9	0.3588304171640134	0.8633325405597306
>10	4.702962813368326	30.89708426980879
>50	1.3173518493458605	23.930879115918245
>100	0.16096083897345065	6.6131831304243525
>500	0.0038454807945875577	0.7677760077692816
>1k	0.00274677199613397	1.3262787157927278
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT	4866	0.7108288182215255	TruSeq Adapter, Index 21 (95% over 23bp)
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1103	0.1611270420259644	No Hit
AAACTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCT	1029	0.15031706821823876	No Hit
AATCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCT	1021	0.14914842240118734	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1012	0.1478336958570045	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCC	1000	0.14608072713142736	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGT	904	0.13205697732681035	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC	888	0.1297196856927075	TruSeq Adapter, Index 14 (95% over 21bp)
AAAAACTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTT	761	0.11116743334701623	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7970164472290677	0.0
2	0.0	0.0	0.0	1.095167211304311	0.0
3	0.0	0.0	0.0	1.4504355396879423	0.0
4	0.0	0.0	0.0	2.9828223672966154	0.0
5	0.0	0.0	0.0	3.2150907234355848	0.0
6	0.0	0.0	0.0	4.556842202137745	0.0
7	0.0	0.0	0.0	5.51527785284704	0.0
8	0.0	0.0	0.0	6.359332294212428	0.0
9	0.0	0.0	0.0	7.537327277800258	0.0
10	0.0	0.0	0.0	9.347267486958643	0.0
11	0.0	0.0	0.0	10.917489222894355	0.0
12	0.0	0.0	0.0	11.667467675987105	0.0
13	0.0	0.0	0.0	11.968101812423582	0.0
14	0.0	0.0	0.0	12.347473460783899	0.0
15	0.0	0.0	0.0	12.676301177556741	0.0
16	0.0	0.0	0.0	13.351486298358198	0.0
17	0.0	0.0	0.0	14.182831716463152	0.0
18	0.0	0.0	0.0	15.228331480542778	0.0
19	0.0	0.0	0.0	15.660292190670408	0.0
20	0.0	0.0	0.0	16.139729137115754	0.0
21	0.0	0.0	0.0	16.689576994038447	0.0
22	0.0	0.0	0.0	17.271708691657185	0.0
23	0.0	0.0	0.0	17.845659868556563	0.0
24	0.0	0.0	0.0	18.301577817933747	0.0
25	0.0	0.0	0.0	18.665026667036738	0.0
26	0.0	0.0	0.0	19.039577651401718	0.0
27	0.0	0.0	0.0	19.435310341200754	0.0
28	0.0	0.0	0.0	19.830166546637003	0.0
29	0.0	0.0	0.0	20.24445148878173	0.0
30	0.0	0.0	0.0	20.771364671544788	0.0
31	0.0	0.0	0.0	21.135251762829174	0.0
32	0.0	0.0	0.0	21.512724361736783	0.0
33	0.0	0.0	0.0	21.9278857882443	0.0
34	0.0	0.0	0.0	22.32814698058441	0.0
35	0.0	0.0	0.0	22.743746649273323	0.0
36	0.0	0.0	0.0	23.125893831449137	0.0
37	0.0	0.0	0.0	23.49503982891025	0.0
38	0.0	0.0	0.0	23.871051620546545	0.0
39	0.0	0.0	0.0	24.274234427429285	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGACG	30	2.16421E-6	45.000004	1
CTAAGCG	30	2.16421E-6	45.000004	1
TCTAGCG	30	2.16421E-6	45.000004	1
ATACACG	60	0.0	45.000004	1
TAATACG	20	7.031285E-4	45.0	1
ATCTCGT	20	7.031285E-4	45.0	19
CGTATTA	20	7.031285E-4	45.0	31
TCCGCGA	25	3.8891463E-5	45.0	33
GTATTCG	25	3.8891463E-5	45.0	1
CGTATAG	20	7.031285E-4	45.0	1
CGAAATC	20	7.031285E-4	45.0	16
CGACGGT	25	3.8891463E-5	45.0	28
GTGATCG	20	7.031285E-4	45.0	27
CGCGAAT	25	3.8891463E-5	45.0	35
TGACGGA	25	3.8891463E-5	45.0	15
CATTCCG	20	7.031285E-4	45.0	1
ATCCGTG	20	7.031285E-4	45.0	15
TTATACG	25	3.8891463E-5	45.0	1
ATTCCGG	40	6.8084773E-9	45.0	2
ACGATTG	20	7.031285E-4	45.0	1
>>END_MODULE