SRR3548516_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548516_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	727373
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	3853	0.5297144656180529	Illumina Single End Adapter 2 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT	1798	0.24719091855210465	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCC	1742	0.23949198004325156	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGT	1574	0.21639516451669227	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	939	0.12909470106809023	No Hit
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	851	0.1169963691256068	TruSeq Adapter, Index 27 (95% over 22bp)
AAACTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT	744	0.10228589733190535	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	55	1.8189894E-12	45.000004	1
CCGGGTT	35	1.2111013E-7	45.0	5
CGAAACG	35	1.2111013E-7	45.0	1
TAACGCG	25	3.8893886E-5	45.0	1
ATCGTTA	20	7.031577E-4	45.0	6
TAGTTCG	20	7.031577E-4	45.0	38
ACTACCG	20	7.031577E-4	45.0	1
CGAATCA	25	3.8893886E-5	45.0	27
ATGCGTA	20	7.031577E-4	45.0	10
TATAGCG	40	6.8084773E-9	45.0	1
CGGCTAA	20	7.031577E-4	45.0	29
CGTCGAA	25	3.8893886E-5	45.0	40
CGGTCTA	25	3.8893886E-5	45.0	31
GATTCGC	25	3.8893886E-5	45.0	10
GCCCGTA	20	7.031577E-4	45.0	44
CCGCAAT	20	7.031577E-4	45.0	9
AATCGTC	20	7.031577E-4	45.0	24
GGTACGA	30	2.1643991E-6	44.999996	27
CGACACG	30	2.1643991E-6	44.999996	1
TTAACGG	60	0.0	44.999996	2