##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548516_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	727373
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.945656492611082	33.0	31.0	34.0	30.0	34.0
2	32.15108891861534	34.0	31.0	34.0	30.0	34.0
3	32.28133983527021	34.0	31.0	34.0	30.0	34.0
4	35.89192752549242	37.0	35.0	37.0	35.0	37.0
5	35.705269510966176	37.0	35.0	37.0	33.0	37.0
6	35.70600228493497	37.0	35.0	37.0	33.0	37.0
7	35.73947479491265	37.0	35.0	37.0	35.0	37.0
8	35.91592346705198	37.0	35.0	37.0	35.0	37.0
9	37.53184129738112	39.0	37.0	39.0	35.0	39.0
10	37.112361883105365	39.0	37.0	39.0	33.0	39.0
11	37.111498502144016	39.0	37.0	39.0	33.0	39.0
12	37.046255497523276	39.0	37.0	39.0	33.0	39.0
13	36.982348808658	39.0	37.0	39.0	33.0	39.0
14	38.1900730436791	40.0	37.0	41.0	33.0	41.0
15	38.17778636270524	40.0	37.0	41.0	33.0	41.0
16	38.111244162211136	40.0	37.0	41.0	33.0	41.0
17	38.13396290486449	40.0	37.0	41.0	33.0	41.0
18	38.11152049911119	40.0	37.0	41.0	33.0	41.0
19	38.088324697232366	40.0	37.0	41.0	33.0	41.0
20	38.023077568180284	40.0	37.0	41.0	33.0	41.0
21	37.997757684159296	40.0	37.0	41.0	33.0	41.0
22	37.98256740351924	40.0	37.0	41.0	33.0	41.0
23	37.86174081248548	40.0	37.0	41.0	33.0	41.0
24	37.75356110276296	40.0	37.0	41.0	33.0	41.0
25	37.73907609988273	40.0	37.0	41.0	33.0	41.0
26	37.68069202458711	40.0	37.0	41.0	33.0	41.0
27	37.64679744780189	40.0	37.0	41.0	33.0	41.0
28	37.54759662511531	40.0	36.0	41.0	32.0	41.0
29	37.435159127435305	39.0	36.0	41.0	32.0	41.0
30	37.43199431378399	39.0	36.0	41.0	32.0	41.0
31	37.343107319078385	39.0	36.0	41.0	32.0	41.0
32	37.320930251741544	39.0	36.0	41.0	32.0	41.0
33	37.268969290859026	39.0	36.0	41.0	32.0	41.0
34	37.208659106125744	39.0	36.0	41.0	32.0	41.0
35	37.18818405412354	39.0	36.0	41.0	31.0	41.0
36	37.06224316822318	39.0	35.0	41.0	31.0	41.0
37	36.93940522950398	39.0	35.0	41.0	31.0	41.0
38	36.90682084707571	39.0	35.0	41.0	31.0	41.0
39	36.843466832010535	39.0	35.0	41.0	31.0	41.0
40	36.75274309054639	39.0	35.0	41.0	31.0	41.0
41	36.70181186268943	39.0	35.0	40.0	31.0	41.0
42	36.642733508117566	39.0	35.0	40.0	31.0	41.0
43	36.55473876539272	39.0	35.0	40.0	30.0	41.0
44	36.41163887029076	39.0	35.0	40.0	30.0	41.0
45	36.19205964477648	38.0	35.0	40.0	30.0	41.0
46	36.047578065174264	38.0	35.0	40.0	30.0	41.0
47	35.956110551257744	38.0	35.0	40.0	29.0	41.0
48	35.88774810173047	38.0	35.0	40.0	29.0	41.0
49	35.71639585192192	38.0	34.0	40.0	28.0	41.0
50	35.584235323554765	38.0	34.0	40.0	28.0	41.0
51	34.30998538576494	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	10.0
11	9.0
12	7.0
13	12.0
14	20.0
15	40.0
16	69.0
17	108.0
18	191.0
19	364.0
20	625.0
21	970.0
22	1379.0
23	1904.0
24	2473.0
25	3209.0
26	4237.0
27	5501.0
28	7228.0
29	9520.0
30	12501.0
31	16236.0
32	21334.0
33	29101.0
34	43942.0
35	54173.0
36	66797.0
37	98156.0
38	152648.0
39	194547.0
40	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.569813561955144	32.26267678343848	19.393626103800937	26.773883550805433
2	24.973844231226618	30.349765526078095	19.542793037409968	25.133597205285323
3	22.039861254129587	29.422318397850894	20.982632019610296	27.55518832840922
4	22.2598309258111	30.357052021452542	19.084431234043606	28.298685818692746
5	19.290652801245027	33.072440137316065	17.618470853331097	30.018436208107808
6	21.322897605492646	37.07066388221724	18.73715411487641	22.869284397413704
7	84.44415726181754	4.940793788056472	5.63067367086763	4.984375279258373
8	85.95246180432873	3.729585783360119	4.784340359072993	5.53361205323816
9	78.04125256230297	7.83655703469884	8.71093647963287	5.411253923365316
10	33.02569658208375	34.916748353320784	13.792923300699917	18.264631763895554
11	25.60048283342934	27.90933949981646	26.237569995036935	20.252607671717264
12	27.217122439243692	23.264817363306033	30.291198600992885	19.22686159645739
13	26.23702007085773	24.237908198407148	28.201349238973677	21.323722491761448
14	22.0707944892098	26.4215196329806	29.31865769007098	22.189028187738614
15	20.24642102470122	28.957907428513295	28.4807107220092	22.314960824776282
16	22.064057918014555	25.656025175528924	28.80076659430581	23.47915031215071
17	21.71403117795134	25.335831822187515	29.14226950959137	23.80786749026978
18	22.471414253759765	25.507545647143896	27.299198622989856	24.721841476106484
19	22.583323824227737	28.881605448648767	27.435717300477197	21.0993534266463
20	24.071693615242797	27.377700299571195	26.433205521788683	22.11740056339732
21	23.981781011942978	26.88373090560139	25.823064644962074	23.311423437493556
22	22.0272129980079	25.67087312836743	26.05540761067568	26.246506262948994
23	22.779096832024283	28.25111737719162	24.767622664025197	24.2021631267589
24	20.929014412137924	28.1755028025511	26.214335698465575	24.6811470868454
25	23.588172780677862	26.614543019881133	25.036948030790256	24.76033616865075
26	24.57844874637909	26.704455623180955	24.57253706145265	24.1445585689873
27	22.390162956282403	24.722253919240885	26.483798546275434	26.40378457820128
28	21.718980495564175	27.222896643125328	28.3212327100401	22.736890151270394
29	22.124962020861375	28.21358505196096	24.906753481363758	24.75469944581391
30	24.255368291096865	24.901529201661322	28.003651496549914	22.8394510106919
31	24.780133439102084	27.200074789688372	23.56892543440573	24.450866336803813
32	23.908778577153676	25.053033313031964	25.94556025587972	25.09262785393464
33	22.163456713405637	25.517444282369567	27.332331554786883	24.986767449437906
34	21.674436637048665	25.738651283454296	28.12202267612353	24.464889403373512
35	22.393325020312822	23.705306630848273	28.63290223860385	25.26846611023505
36	25.332944720246697	26.425231621190225	26.85472240514839	21.387101253414688
37	22.606008196619893	28.088202339102498	24.47478803859918	24.831001425678433
38	22.332283436421204	26.726727552438707	27.71617863187113	23.22481037926896
39	22.561464338104383	23.306749081970324	27.939997772807075	26.191788807118215
40	24.084479352409286	23.90520406998885	28.499958068281334	23.51035850932053
41	22.103927421006826	23.24227047195868	29.528178802347625	25.12562330468687
42	23.813366732061816	25.08561632064979	26.219010053988807	24.882006893299586
43	22.34011985597486	24.62862932773144	26.776908133791054	26.254342682502653
44	23.879907557745476	23.237321154345842	26.852385227386772	26.030386060521906
45	24.823852411348785	23.62543014381892	28.188975944941593	23.361741499890705
46	21.89770585380541	23.90369177849604	30.425242619673813	23.773359748024742
47	22.555277691088342	25.49709708773903	27.553813517961213	24.39381170321142
48	22.608207893336708	24.125448703759968	29.169353275417155	24.096990127486173
49	22.080555643390667	24.51259532591944	28.275176560031785	25.131672470658106
50	21.361254816992105	23.18411599000788	28.7120913204092	26.742537872590816
51	22.69317117902369	21.581499450763225	30.299035020546544	25.426294349666538
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	976.0
1	847.5
2	719.0
3	523.0
4	327.0
5	281.0
6	235.0
7	252.0
8	269.0
9	308.5
10	348.0
11	439.5
12	531.0
13	640.5
14	750.0
15	930.0
16	1110.0
17	1094.0
18	1078.0
19	1187.5
20	1297.0
21	1256.0
22	1215.0
23	1597.0
24	1979.0
25	3050.0
26	4795.0
27	5469.0
28	6813.5
29	8158.0
30	9913.5
31	11669.0
32	12325.0
33	12981.0
34	15514.0
35	18047.0
36	19947.5
37	21848.0
38	23214.5
39	24581.0
40	27882.0
41	31183.0
42	33275.5
43	35368.0
44	43367.5
45	51367.0
46	57288.0
47	63209.0
48	67568.5
49	71928.0
50	68575.5
51	65223.0
52	59338.5
53	53454.0
54	49276.5
55	45099.0
56	41664.5
57	38230.0
58	36234.0
59	34238.0
60	33147.5
61	32057.0
62	29249.0
63	26441.0
64	23106.5
65	19772.0
66	16436.5
67	13101.0
68	10942.5
69	8784.0
70	7583.5
71	6383.0
72	6124.5
73	5866.0
74	4716.0
75	3033.0
76	2500.0
77	1684.0
78	868.0
79	712.5
80	557.0
81	397.0
82	237.0
83	160.0
84	83.0
85	85.5
86	88.0
87	55.5
88	23.0
89	23.0
90	23.0
91	14.5
92	6.0
93	6.5
94	7.0
95	4.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	727373.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.622954713172525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.85723978871631	19.66297950134918
2	10.432937851953747	5.555112639158161
3	3.7737418483160377	3.014044749807655
4	1.821362430674924	1.9396019803252937
5	1.103096079625918	1.4683838486079483
6	0.731761943120745	1.1688999043516035
7	0.5866772156655441	1.093335666073975
8	0.4807789548020541	1.0239805072593204
9	0.3817139346567976	0.9146117516179336
>10	5.520941020639899	36.19148237621799
>50	1.1951588629705743	21.038415354575974
>100	0.10836798367063077	4.923678850353045
>500	0.0041480567912203165	0.7676510622073925
>1k	0.0020740283956101582	1.2378218080945314
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	3853	0.5297144656180529	Illumina Single End Adapter 2 (95% over 22bp)
AATCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT	1798	0.24719091855210465	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCC	1742	0.23949198004325156	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGT	1574	0.21639516451669227	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	939	0.12909470106809023	No Hit
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	851	0.1169963691256068	TruSeq Adapter, Index 27 (95% over 22bp)
AAACTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT	744	0.10228589733190535	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6042291919001668	0.0
2	0.0	0.0	0.0	0.865443177021968	0.0
3	0.0	0.0	0.0	1.194160355141035	0.0
4	0.0	0.0	0.0	2.6405984274918097	0.0
5	0.0	0.0	0.0	2.85383152797808	0.0
6	0.0	0.0	0.0	4.085661689394574	0.0
7	0.0	0.0	0.0	4.933507292682021	0.0
8	0.0	0.0	0.0	5.678929517592762	0.0
9	0.0	0.0	0.0	6.871990024375389	0.0
10	0.0	0.0	0.0	8.543347086020514	0.0
11	0.0	0.0	0.0	10.150225537653997	0.0
12	0.0	0.0	0.0	10.861552463454101	0.0
13	0.0	0.0	0.0	11.15727419082094	0.0
14	0.0	0.0	0.0	11.517474528199424	0.0
15	0.0	0.0	0.0	11.820070307806311	0.0
16	0.0	0.0	0.0	12.439972338813787	0.0
17	0.0	0.0	0.0	13.154323847599512	0.0
18	0.0	0.0	0.0	14.100055954785233	0.0
19	0.0	0.0	0.0	14.499025946797586	0.0
20	0.0	0.0	0.0	14.94927636852069	0.0
21	0.0	0.0	0.0	15.426610556069582	0.0
22	0.0	0.0	0.0	15.974335038556559	0.0
23	0.0	0.0	0.0	16.517522646565105	0.0
24	0.0	0.0	0.0	16.904531787679772	0.0
25	0.0	0.0	0.0	17.268581594312685	0.0
26	0.0	0.0	0.0	17.611184357956645	0.0
27	0.0	0.0	0.0	17.98430791354642	0.0
28	0.0	0.0	0.0	18.352069708388957	0.0
29	0.0	0.0	0.0	18.728767771143552	0.0
30	0.0	0.0	0.0	19.219712582127737	0.0
31	0.0	0.0	0.0	19.571389094728563	0.0
32	0.0	0.0	0.0	19.926777595539015	0.0
33	0.0	0.0	0.0	20.317223762773708	0.0
34	0.0	0.0	0.0	20.653639879401627	0.0
35	0.0	0.0	0.0	21.067870267386883	0.0
36	0.0	0.0	0.0	21.41954677998771	0.0
37	0.0	0.0	0.0	21.757062744974036	0.0
38	0.0	0.0	0.0	22.112313764739685	0.0
39	0.0	0.0	0.0	22.478563268089413	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	55	1.8189894E-12	45.000004	1
CCGGGTT	35	1.2111013E-7	45.0	5
CGAAACG	35	1.2111013E-7	45.0	1
TAACGCG	25	3.8893886E-5	45.0	1
ATCGTTA	20	7.031577E-4	45.0	6
TAGTTCG	20	7.031577E-4	45.0	38
ACTACCG	20	7.031577E-4	45.0	1
CGAATCA	25	3.8893886E-5	45.0	27
ATGCGTA	20	7.031577E-4	45.0	10
TATAGCG	40	6.8084773E-9	45.0	1
CGGCTAA	20	7.031577E-4	45.0	29
CGTCGAA	25	3.8893886E-5	45.0	40
CGGTCTA	25	3.8893886E-5	45.0	31
GATTCGC	25	3.8893886E-5	45.0	10
GCCCGTA	20	7.031577E-4	45.0	44
CCGCAAT	20	7.031577E-4	45.0	9
AATCGTC	20	7.031577E-4	45.0	24
GGTACGA	30	2.1643991E-6	44.999996	27
CGACACG	30	2.1643991E-6	44.999996	1
TTAACGG	60	0.0	44.999996	2
>>END_MODULE