SRR3548514_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548514_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	724765
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	5316	0.7334791277172601	RNA PCR Primer, Index 16 (95% over 24bp)
AATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	1587	0.21896752740543485	RNA PCR Primer, Index 14 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCC	1407	0.1941318910267466	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGT	1321	0.18226597586804	No Hit
AAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	1316	0.1815760970797431	RNA PCR Primer, Index 14 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1177	0.16239746676508937	No Hit
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	1112	0.15342904251722972	RNA PCR Primer, Index 16 (95% over 23bp)
AAAAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	1001	0.13811373341703861	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	811	0.11189833946175656	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGT	20	7.031559E-4	45.000004	40
TATCACG	20	7.031559E-4	45.000004	1
TCGCACG	20	7.031559E-4	45.000004	36
GTCGAAT	20	7.031559E-4	45.000004	22
GTCGAAC	20	7.031559E-4	45.000004	9
GACGTCG	20	7.031559E-4	45.000004	12
ATTACCG	30	2.1643864E-6	45.000004	1
TTCACGG	60	0.0	45.000004	2
GCCGGAC	20	7.031559E-4	45.000004	19
CGTTAGA	20	7.031559E-4	45.000004	23
TCGCTAA	20	7.031559E-4	45.000004	15
GATATAC	20	7.031559E-4	45.000004	29
TTGCGAA	20	7.031559E-4	45.000004	38
ACGTCGC	20	7.031559E-4	45.000004	13
CGTAACG	20	7.031559E-4	45.000004	1
CGTAAAG	20	7.031559E-4	45.000004	1
TTACGCA	20	7.031559E-4	45.000004	33
CGACTAG	40	6.8084773E-9	45.000004	1
CGGTAAT	40	6.8084773E-9	45.000004	6
AGAATCG	40	6.8084773E-9	45.000004	45