##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548514_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	724765
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.872532476043958	33.0	31.0	34.0	30.0	34.0
2	32.08139051968569	34.0	31.0	34.0	30.0	34.0
3	32.21926969431471	34.0	31.0	34.0	30.0	34.0
4	35.842953233116944	37.0	35.0	37.0	35.0	37.0
5	35.64496216014846	37.0	35.0	37.0	33.0	37.0
6	35.63753354535608	37.0	35.0	37.0	33.0	37.0
7	35.6628272612502	37.0	35.0	37.0	35.0	37.0
8	35.86022917773347	37.0	35.0	37.0	35.0	37.0
9	37.480530930715474	39.0	37.0	39.0	35.0	39.0
10	37.06914931046615	39.0	37.0	39.0	33.0	39.0
11	37.06329499906867	39.0	37.0	39.0	33.0	39.0
12	36.92361938007492	39.0	37.0	39.0	33.0	39.0
13	36.874595213620964	39.0	37.0	39.0	33.0	39.0
14	38.0444530296027	40.0	37.0	41.0	33.0	41.0
15	38.017022069222435	40.0	37.0	41.0	33.0	41.0
16	37.973707339620425	40.0	37.0	41.0	33.0	41.0
17	37.978768290411374	40.0	37.0	41.0	33.0	41.0
18	37.95748966906515	40.0	37.0	41.0	33.0	41.0
19	37.94324781135954	40.0	37.0	41.0	33.0	41.0
20	37.884953053748454	40.0	37.0	41.0	33.0	41.0
21	37.84142170220692	40.0	37.0	41.0	33.0	41.0
22	37.83554807420336	40.0	37.0	41.0	33.0	41.0
23	37.731870330382954	39.0	36.0	41.0	33.0	41.0
24	37.60243113284996	39.0	36.0	41.0	32.0	41.0
25	37.59159865611612	39.0	36.0	41.0	32.0	41.0
26	37.53117907183708	39.0	36.0	41.0	32.0	41.0
27	37.480104585624304	39.0	36.0	41.0	32.0	41.0
28	37.44178319869199	39.0	36.0	41.0	32.0	41.0
29	37.30997909667271	39.0	36.0	41.0	32.0	41.0
30	37.231586790200964	39.0	36.0	41.0	32.0	41.0
31	37.180882079018716	39.0	36.0	41.0	31.0	41.0
32	37.11465233558464	39.0	35.0	41.0	31.0	41.0
33	37.006057135761246	39.0	35.0	41.0	31.0	41.0
34	36.96837181707174	39.0	35.0	41.0	31.0	41.0
35	36.88352086538395	39.0	35.0	41.0	31.0	41.0
36	36.7898256676302	39.0	35.0	41.0	31.0	41.0
37	36.74015853414555	39.0	35.0	40.0	31.0	41.0
38	36.66284657785627	39.0	35.0	40.0	30.0	41.0
39	36.5499410153636	39.0	35.0	40.0	30.0	41.0
40	36.422094058073995	39.0	35.0	40.0	30.0	41.0
41	36.42084675722476	39.0	35.0	40.0	30.0	41.0
42	36.3876152959925	39.0	35.0	40.0	30.0	41.0
43	36.306041268549116	38.0	35.0	40.0	30.0	41.0
44	36.191245438176516	38.0	35.0	40.0	30.0	41.0
45	35.97659517222824	38.0	35.0	40.0	29.0	41.0
46	35.79494870751209	38.0	35.0	40.0	29.0	41.0
47	35.70599849606424	38.0	34.0	40.0	29.0	41.0
48	35.65565528136706	38.0	34.0	40.0	28.0	41.0
49	35.52403744662063	38.0	34.0	40.0	28.0	41.0
50	35.369842638648386	38.0	34.0	40.0	28.0	41.0
51	34.073121632529165	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	6.0
11	3.0
12	3.0
13	7.0
14	12.0
15	22.0
16	48.0
17	105.0
18	232.0
19	335.0
20	659.0
21	1056.0
22	1452.0
23	2022.0
24	2647.0
25	3502.0
26	4623.0
27	5943.0
28	7719.0
29	10416.0
30	13450.0
31	17555.0
32	23202.0
33	31008.0
34	47923.0
35	58859.0
36	67890.0
37	96655.0
38	147769.0
39	179568.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.11125675218864	32.265354977130514	19.77330583016564	26.850082440515198
2	24.048622656999168	30.85620856415528	19.635192096748604	25.459976682096958
3	21.674887722227204	29.93356467268701	20.907466558125737	27.48408104696005
4	22.121653225528277	31.08883569156899	18.986981987264837	27.802529095637894
5	18.630728580988322	33.85966485688465	17.536028919718806	29.973577642408227
6	20.75803881258063	37.2086124467931	20.171779818285927	21.861568922340343
7	82.32696115292542	5.484812318475644	6.680096307078847	5.508130221520078
8	84.21350368740212	4.088773602478045	5.278262609259553	6.419460100860279
9	75.97028002180018	8.231357750443246	10.100377363697199	5.697984864059385
10	33.86021675991528	33.33935827475113	14.090774250964106	18.709650714369484
11	26.55495229488179	27.05442453760874	26.598966561575132	19.79165660593434
12	27.78252261077729	23.070650486709486	30.306513145640313	18.840313756872916
13	26.79309845260188	23.568880947617505	28.09738328975599	21.54063731002463
14	21.3739625947721	25.94123612481287	29.958952212096335	22.725849068318695
15	19.975992218167267	29.76316461197768	28.730554041654884	21.530289128200174
16	22.720054086497	25.732202851958913	28.08634522914324	23.461397832400845
17	20.655108897366734	26.731285313170478	29.48610929059764	23.12749649886515
18	22.09805937096852	26.24188530075266	27.81149751988576	23.848557808393064
19	21.275033976530324	30.808606927762796	27.367491531737876	20.54886756396901
20	23.44125337178258	28.03805371396246	26.258304415914125	22.26238849834084
21	22.85996150476361	28.22887418680538	26.132194573413454	22.778969735017558
22	20.943064303601858	26.642014997964857	26.24795623408967	26.166964464343618
23	22.787248280477122	27.489876028781744	24.726635530137354	24.996240160603783
24	21.126296109773513	28.31055583533973	26.279276731078347	24.283871323808405
25	23.234703662566485	25.67632267010686	25.897359833877186	25.191613833449463
26	23.481404317261457	26.782750270777424	25.666526391313045	24.069319020648074
27	19.930184266624355	26.06762191882886	27.489462101508767	26.51273171303802
28	20.932992073292723	26.265065228039436	28.816374962919017	23.985567735748827
29	21.728698267714364	28.842866308389613	23.28727242623471	26.14116299766131
30	22.783936862293295	27.617503604616665	25.89308258538975	23.705476947700287
31	22.506053686367306	27.036073761840047	22.892247832055908	27.565624719736743
32	21.831904134443576	26.733354949535364	24.701247990728028	26.733492925293028
33	20.700778873151986	27.53168268335254	25.76283347015929	26.004704973336185
34	18.42611053237946	27.42764896207736	26.629459204017852	27.516781301525324
35	20.256910860761764	26.253751215911365	26.17262147040765	27.31671645291922
36	20.47767207301677	30.31092836988541	25.067021724283045	24.144377832814776
37	20.133974460687256	30.64924492766621	21.70882975861141	27.507950853035123
38	20.592743854904693	28.51779542334412	24.394113954178252	26.495346767572936
39	20.700088994363693	26.1350920643243	24.388870875387195	28.775948065924815
40	20.85089649748539	27.777141556228568	24.997481942422716	26.374480003863322
41	18.874807696287764	25.566355991252337	28.18651562920395	27.372320683255953
42	21.192938400722994	26.330465737169977	24.68731243920443	27.789283422902596
43	19.57765620580464	25.418032051768503	26.622008513104245	28.38230322932261
44	21.55553869185184	23.72341379619601	26.24340303408691	28.477644477865237
45	23.72962270529068	23.65552972342759	27.17680903465261	25.438038536629115
46	19.700316654363828	25.599608148848247	29.5001828178789	25.199892378909027
47	21.31325326140197	26.221464888619067	26.111498209764544	26.353783640214413
48	20.83102798838244	24.28304346926245	29.34620187233103	25.539726670024077
49	20.739412085296614	23.722447965892393	27.90821852600498	27.629921422806014
50	19.869750884769548	23.136878850385987	27.27159838016461	29.72177188467986
51	20.243527212268805	22.158906680096308	30.342800769904727	27.254765337730163
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1526.0
1	1306.5
2	1087.0
3	731.0
4	375.0
5	356.5
6	338.0
7	330.5
8	323.0
9	358.0
10	393.0
11	534.0
12	675.0
13	819.5
14	964.0
15	1024.0
16	1084.0
17	1171.0
18	1258.0
19	1345.5
20	1433.0
21	1600.5
22	1768.0
23	2611.0
24	3454.0
25	3176.5
26	4271.0
27	5643.0
28	6502.5
29	7362.0
30	8001.0
31	8640.0
32	11280.0
33	13920.0
34	15300.5
35	16681.0
36	17887.5
37	19094.0
38	22030.5
39	24967.0
40	26191.0
41	27415.0
42	31685.5
43	35956.0
44	42788.0
45	49620.0
46	57915.5
47	66211.0
48	72226.5
49	78242.0
50	74999.0
51	71756.0
52	65718.5
53	59681.0
54	52859.0
55	46037.0
56	42189.5
57	38342.0
58	36070.5
59	33799.0
60	30668.0
61	27537.0
62	25873.5
63	24210.0
64	20775.0
65	17340.0
66	14081.5
67	10823.0
68	9592.5
69	8362.0
70	7076.5
71	5791.0
72	4808.0
73	3825.0
74	3231.5
75	2183.5
76	1729.0
77	1192.0
78	655.0
79	511.5
80	368.0
81	323.0
82	278.0
83	191.0
84	104.0
85	73.0
86	42.0
87	45.0
88	48.0
89	42.5
90	37.0
91	31.0
92	25.0
93	15.5
94	6.0
95	5.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	724765.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.587123923710056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.58290895660971	20.29940828053067
2	10.961147889441904	6.047730903442935
3	4.135055814274872	3.4222289153957606
4	2.041735415057673	2.253024316584945
5	1.2304143654218158	1.6971796788202351
6	0.7962695356513209	1.3180071814012828
7	0.586623303445144	1.132827483807415
8	0.4095914201577986	0.9039559412785267
9	0.36002225655973136	0.893878074630634
>10	4.5313446678829825	30.042791958603022
>50	1.1993544005195718	22.671542443102727
>100	0.1575061822513146	6.700099136384689
>500	0.004012896363090818	0.6472048341705811
>1k	0.0035112843177044664	1.2344909826033255
>5k	5.016120453863522E-4	0.7356298692432774
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	5316	0.7334791277172601	RNA PCR Primer, Index 16 (95% over 24bp)
AATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	1587	0.21896752740543485	RNA PCR Primer, Index 14 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCC	1407	0.1941318910267466	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGT	1321	0.18226597586804	No Hit
AAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	1316	0.1815760970797431	RNA PCR Primer, Index 14 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1177	0.16239746676508937	No Hit
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	1112	0.15342904251722972	RNA PCR Primer, Index 16 (95% over 23bp)
AAAAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	1001	0.13811373341703861	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	811	0.11189833946175656	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8809752126551365	0.0
2	0.0	0.0	0.0	1.258476885611198	0.0
3	0.0	0.0	0.0	1.6548812373665946	0.0
4	0.0	0.0	0.0	3.50968934758163	0.0
5	0.0	0.0	0.0	3.783709202293157	0.0
6	0.0	0.0	0.0	5.358840451732631	0.0
7	0.0	0.0	0.0	6.516181106979504	0.0
8	0.0	0.0	0.0	7.485874731809621	0.0
9	0.0	0.0	0.0	8.958352017550517	0.0
10	0.0	0.0	0.0	11.011155340006761	0.0
11	0.0	0.0	0.0	12.890523135085166	0.0
12	0.0	0.0	0.0	13.778121184107953	0.0
13	0.0	0.0	0.0	14.14306706311701	0.0
14	0.0	0.0	0.0	14.61646188764634	0.0
15	0.0	0.0	0.0	14.99658509999793	0.0
16	0.0	0.0	0.0	15.76359233682642	0.0
17	0.0	0.0	0.0	16.698378094968714	0.0
18	0.0	0.0	0.0	17.864687174463448	0.0
19	0.0	0.0	0.0	18.37961270204825	0.0
20	0.0	0.0	0.0	18.967803356950185	0.0
21	1.3797575765937925E-4	0.0	0.0	19.56206494518913	0.0
22	1.3797575765937925E-4	0.0	0.0	20.21096493346119	0.0
23	1.3797575765937925E-4	0.0	0.0	20.881527115685774	0.0
24	1.3797575765937925E-4	0.0	0.0	21.36209667961339	0.0
25	2.759515153187585E-4	0.0	0.0	21.78499237683939	0.0
26	2.759515153187585E-4	0.0	0.0	22.17160044980097	0.0
27	2.759515153187585E-4	0.0	0.0	22.590494850054846	0.0
28	2.759515153187585E-4	0.0	0.0	23.00511200182128	0.0
29	2.759515153187585E-4	0.0	0.0	23.438217905114072	0.0
30	2.759515153187585E-4	0.0	0.0	23.973563844832462	0.0
31	2.759515153187585E-4	0.0	0.0	24.37189985719509	0.0
32	2.759515153187585E-4	0.0	0.0	24.798244948362573	0.0
33	2.759515153187585E-4	0.0	0.0	25.23438631832387	0.0
34	2.759515153187585E-4	0.0	0.0	25.645416100391163	0.0
35	2.759515153187585E-4	0.0	0.0	26.077832124895657	0.0
36	2.759515153187585E-4	0.0	0.0	26.49286320393507	0.0
37	2.759515153187585E-4	0.0	0.0	26.87478010113623	0.0
38	2.759515153187585E-4	0.0	0.0	27.311335398370506	0.0
39	2.759515153187585E-4	0.0	0.0	27.73947417438756	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGT	20	7.031559E-4	45.000004	40
TATCACG	20	7.031559E-4	45.000004	1
TCGCACG	20	7.031559E-4	45.000004	36
GTCGAAT	20	7.031559E-4	45.000004	22
GTCGAAC	20	7.031559E-4	45.000004	9
GACGTCG	20	7.031559E-4	45.000004	12
ATTACCG	30	2.1643864E-6	45.000004	1
TTCACGG	60	0.0	45.000004	2
GCCGGAC	20	7.031559E-4	45.000004	19
CGTTAGA	20	7.031559E-4	45.000004	23
TCGCTAA	20	7.031559E-4	45.000004	15
GATATAC	20	7.031559E-4	45.000004	29
TTGCGAA	20	7.031559E-4	45.000004	38
ACGTCGC	20	7.031559E-4	45.000004	13
CGTAACG	20	7.031559E-4	45.000004	1
CGTAAAG	20	7.031559E-4	45.000004	1
TTACGCA	20	7.031559E-4	45.000004	33
CGACTAG	40	6.8084773E-9	45.000004	1
CGGTAAT	40	6.8084773E-9	45.000004	6
AGAATCG	40	6.8084773E-9	45.000004	45
>>END_MODULE