SRR3548513_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548513_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	732022
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	5537	0.756398031753144	RNA PCR Primer, Index 16 (95% over 24bp)
AATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	1478	0.20190650007786654	RNA PCR Primer, Index 14 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCC	1439	0.19657879134780104	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGT	1361	0.18592337388767002	No Hit
AAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	1250	0.17075989519440674	RNA PCR Primer, Index 14 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1205	0.16461253896740807	No Hit
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	1070	0.14617047028641217	RNA PCR Primer, Index 16 (95% over 23bp)
AAAAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	916	0.12513285119846124	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	862	0.11775602372606286	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	40	6.8084773E-9	45.000004	1
GTATTCG	20	7.031605E-4	45.000004	29
CGACGTA	20	7.031605E-4	45.000004	24
CGAGTAC	20	7.031605E-4	45.000004	26
CGAAACG	35	1.2111195E-7	45.000004	1
CGACGAT	20	7.031605E-4	45.000004	34
TTTCGCG	20	7.031605E-4	45.000004	1
ATAGCTA	20	7.031605E-4	45.000004	27
TGACGTA	30	2.1644173E-6	45.000004	18
TAGCCGC	20	7.031605E-4	45.000004	22
CCGTCTA	20	7.031605E-4	45.000004	29
CGTTAAC	20	7.031605E-4	45.000004	45
CCCGTCA	30	2.1644173E-6	45.000004	35
TGCGCTT	20	7.031605E-4	45.000004	38
CGTAGAT	20	7.031605E-4	45.000004	27
TCCGTTG	20	7.031605E-4	45.000004	40
GCGATGT	30	2.1644173E-6	45.000004	9
CGTCGTC	20	7.031605E-4	45.000004	19
CGTCGAA	20	7.031605E-4	45.000004	21
CGGTATC	20	7.031605E-4	45.000004	29