##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548513_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	732022
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.850269527418575	33.0	31.0	34.0	30.0	34.0
2	32.04607238580261	34.0	31.0	34.0	30.0	34.0
3	32.19617306583682	34.0	31.0	34.0	30.0	34.0
4	35.788362918054375	37.0	35.0	37.0	35.0	37.0
5	35.62128597227952	37.0	35.0	37.0	33.0	37.0
6	35.623009964181406	37.0	35.0	37.0	33.0	37.0
7	35.67614907748674	37.0	35.0	37.0	35.0	37.0
8	35.86944927884681	37.0	35.0	37.0	35.0	37.0
9	37.479247891456815	39.0	37.0	39.0	35.0	39.0
10	37.0538440101527	39.0	37.0	39.0	33.0	39.0
11	37.05868402862209	39.0	37.0	39.0	33.0	39.0
12	36.92674127280328	39.0	37.0	39.0	33.0	39.0
13	36.86681137998585	39.0	37.0	39.0	33.0	39.0
14	38.030641155593685	40.0	37.0	41.0	33.0	41.0
15	37.994266565758956	40.0	37.0	41.0	33.0	41.0
16	37.98227375679966	40.0	37.0	41.0	33.0	41.0
17	37.95275142003929	40.0	37.0	41.0	33.0	41.0
18	37.90990981145375	40.0	37.0	41.0	33.0	41.0
19	37.90703284873952	40.0	37.0	41.0	33.0	41.0
20	37.84987336446172	40.0	37.0	41.0	33.0	41.0
21	37.82811172341815	40.0	37.0	41.0	33.0	41.0
22	37.802158951506925	40.0	37.0	41.0	33.0	41.0
23	37.718803533227145	39.0	36.0	41.0	33.0	41.0
24	37.60294226129816	39.0	36.0	41.0	32.0	41.0
25	37.603466835696196	39.0	36.0	41.0	32.0	41.0
26	37.51244634724093	39.0	36.0	41.0	32.0	41.0
27	37.44691552986112	39.0	36.0	41.0	32.0	41.0
28	37.4049263546724	39.0	36.0	41.0	32.0	41.0
29	37.27417208772414	39.0	36.0	41.0	32.0	41.0
30	37.21578586435927	39.0	36.0	41.0	31.0	41.0
31	37.179868911043656	39.0	36.0	41.0	31.0	41.0
32	37.11960023059416	39.0	35.0	41.0	31.0	41.0
33	37.0155637398876	39.0	35.0	41.0	31.0	41.0
34	36.9561515910724	39.0	35.0	41.0	31.0	41.0
35	36.86713104250965	39.0	35.0	41.0	31.0	41.0
36	36.78583020728885	39.0	35.0	41.0	31.0	41.0
37	36.698375185445244	39.0	35.0	40.0	30.0	41.0
38	36.5912076413004	39.0	35.0	40.0	30.0	41.0
39	36.521411924778214	39.0	35.0	40.0	30.0	41.0
40	36.42060894344706	39.0	35.0	40.0	30.0	41.0
41	36.44501804590573	39.0	35.0	40.0	30.0	41.0
42	36.39983224547896	39.0	35.0	40.0	30.0	41.0
43	36.316461800328405	38.0	35.0	40.0	30.0	41.0
44	36.2492466073424	38.0	35.0	40.0	30.0	41.0
45	36.034990751644074	38.0	35.0	40.0	30.0	41.0
46	35.834778189726535	38.0	35.0	40.0	29.0	41.0
47	35.72900541240564	38.0	34.0	40.0	29.0	41.0
48	35.693796088095716	38.0	34.0	40.0	29.0	41.0
49	35.5631538396387	38.0	34.0	40.0	28.0	41.0
50	35.376734579015384	38.0	34.0	40.0	28.0	41.0
51	34.13759559138933	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	6.0
11	4.0
12	4.0
13	9.0
14	8.0
15	25.0
16	50.0
17	104.0
18	182.0
19	358.0
20	635.0
21	1012.0
22	1467.0
23	2083.0
24	2832.0
25	3536.0
26	4636.0
27	6189.0
28	7824.0
29	10417.0
30	13681.0
31	17725.0
32	23104.0
33	31582.0
34	48416.0
35	59849.0
36	69007.0
37	97603.0
38	149217.0
39	180386.0
40	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.168626079544055	32.16160170049534	19.861424929851836	26.80834729010877
2	24.00228408435812	30.698940742218127	19.663343451426325	25.635431721997428
3	21.598257975853187	29.920685443880103	20.92928901043958	27.551767569827135
4	22.209851616481473	31.02065238476439	18.941643830376684	27.827852168377454
5	18.525126294018484	33.895019548592806	17.467507807142407	30.112346350246305
6	20.73230039534331	37.185357817114784	20.208272428970712	21.87406935857119
7	82.52798413162445	5.5016106073314734	6.514968129373161	5.455437131670906
8	84.3551150102046	4.080341847649387	5.2580386928261715	6.306504449319829
9	76.14716497591603	8.210135760947075	10.037130031611072	5.605569231525828
10	33.9432421429957	33.31730467117108	14.042747349123385	18.696705836709825
11	26.492783003789505	27.208745092360616	26.58267101261984	19.715800891230046
12	27.71009614465139	23.14192742841062	30.167262732540824	18.980713694397163
13	26.737584389540203	23.54888241063793	27.996153121081058	21.717380078740803
14	21.496348470401163	26.01383564974823	29.927652447604036	22.56216343224657
15	19.991339058115738	29.624792697487234	28.7685342790244	21.615333965372628
16	22.725000068303956	25.8090603834311	28.08016698951671	23.385772558748236
17	20.712219031668447	26.643871359057513	29.582444243479024	23.061465365795016
18	22.04933731499873	26.264101352145154	27.800940408894814	23.8856209239613
19	21.35086103969553	30.708639904265173	27.397537232487544	20.54296182355175
20	23.544920781069422	28.07115086705044	26.204540300701346	22.17938805117879
21	22.919256525077113	28.301881637437127	26.04785102087096	22.731010816614802
22	21.0769621678037	26.67365188477942	26.284865755400794	25.96452019201609
23	22.74535464781113	27.467343877643017	24.72316405791083	25.064137416635017
24	21.151003658359997	28.298466439533236	26.316558791948875	24.233971110157892
25	23.21378319230843	25.767531576919822	25.876271478179618	25.142413752592134
26	23.416509339883227	26.748786238664955	25.652371103600714	24.182333317851104
27	20.029862490471597	26.184458937026484	27.288387507479285	26.497291065022637
28	20.921638967134868	26.378852001715796	28.757742253648118	23.941766777501222
29	21.89305785891681	28.725366177519255	23.24574944468882	26.135826518875117
30	22.77855037143692	27.69452284220966	25.83446945583603	23.69245733051739
31	22.592080565884633	27.11284633521943	22.822811336271315	27.472261762624623
32	21.870790768583458	26.71477086754223	24.75936515569204	26.655073208182266
33	20.83147774247222	27.500812817101128	25.738707306611	25.929002133815647
34	18.596299018335515	27.386608599195107	26.67119294228862	27.34589944018076
35	20.325482021032155	26.254265582181958	26.186644663684973	27.233607733100918
36	20.420697738592555	30.294854526230086	25.143916439669844	24.14053129550751
37	20.134913977995197	30.568890006038067	21.816557425869714	27.479638590097018
38	20.52342689154151	28.506793511670413	24.45104108892902	26.51873850785905
39	20.69719216089134	26.035692916333115	24.464292056796108	28.802822865979437
40	20.871777077738102	27.702309493430526	25.077661600334416	26.34825182849696
41	18.849023663223235	25.588301990923767	28.12797976017114	27.43469458568185
42	21.249907789656593	26.26095937007358	24.72248101833005	27.766651821939774
43	19.512801527822933	25.51070869454743	26.637177571165893	28.33931220646374
44	21.63473228946671	23.809803530494985	26.23117884435167	28.324285335686632
45	23.648332973599153	23.772782785216837	27.18156011704566	25.397324124138343
46	19.732330449084863	25.639393351565936	29.411957564116925	25.216318635232277
47	21.36943971629268	26.1997590236359	26.150170350071445	26.280630909999974
48	20.85169571406324	24.319214449838938	29.25526828428654	25.57382155181128
49	20.800194529672606	23.757619306523576	27.8375513304245	27.604634833379325
50	19.851315944056328	23.116518356005695	27.42199004947939	29.610175650458594
51	20.271521894150723	22.147148582966086	30.27163118048365	27.309698342399546
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1554.0
1	1309.0
2	1064.0
3	738.5
4	413.0
5	358.0
6	303.0
7	332.0
8	361.0
9	386.0
10	411.0
11	570.5
12	730.0
13	829.5
14	929.0
15	1038.5
16	1148.0
17	1191.5
18	1235.0
19	1353.0
20	1471.0
21	1601.5
22	1732.0
23	2582.5
24	3433.0
25	3249.5
26	4372.0
27	5678.0
28	6516.5
29	7355.0
30	8108.0
31	8861.0
32	11530.0
33	14199.0
34	15476.5
35	16754.0
36	17995.5
37	19237.0
38	22298.5
39	25360.0
40	26581.5
41	27803.0
42	32217.5
43	36632.0
44	43211.0
45	49790.0
46	58030.0
47	66270.0
48	72318.5
49	78367.0
50	75420.5
51	72474.0
52	66509.5
53	60545.0
54	53369.5
55	46194.0
56	42480.5
57	38767.0
58	36567.5
59	34368.0
60	31150.0
61	27932.0
62	26264.0
63	24596.0
64	21173.0
65	17750.0
66	14306.0
67	10862.0
68	9779.5
69	8697.0
70	7288.5
71	5880.0
72	4822.5
73	3765.0
74	3195.0
75	2170.5
76	1716.0
77	1218.0
78	720.0
79	559.0
80	398.0
81	353.5
82	309.0
83	209.5
84	110.0
85	74.5
86	39.0
87	39.5
88	40.0
89	33.5
90	27.0
91	31.0
92	35.0
93	24.5
94	14.0
95	8.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	732022.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.13192640186489
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.29029859752029	19.885069875186225
2	11.083181582549667	6.014161339924841
3	4.1661497197015205	3.391070027222751
4	2.1116534496803534	2.2917290393188585
5	1.202154191433216	1.6308379522829703
6	0.7723033238486857	1.25724461655469
7	0.587038511868339	1.1149239989350443
8	0.4258243154807902	0.9242747190199437
9	0.3569380006303768	0.8715974006818968
>10	4.581677057984763	29.873455537220266
>50	1.2370250349677165	22.931653908836644
>100	0.1776578507665527	7.223982526693938
>500	0.004555329506834684	0.7580485048161839
>1k	0.00303688633788979	1.0715674788189644
>5k	5.061477229816316E-4	0.7603830744868134
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	5537	0.756398031753144	RNA PCR Primer, Index 16 (95% over 24bp)
AATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	1478	0.20190650007786654	RNA PCR Primer, Index 14 (95% over 21bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCC	1439	0.19657879134780104	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGT	1361	0.18592337388767002	No Hit
AAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	1250	0.17075989519440674	RNA PCR Primer, Index 14 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1205	0.16461253896740807	No Hit
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	1070	0.14617047028641217	RNA PCR Primer, Index 16 (95% over 23bp)
AAAAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	916	0.12513285119846124	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	862	0.11775602372606286	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8976506170579572	0.0
2	0.0	0.0	0.0	1.258705339457011	0.0
3	0.0	0.0	0.0	1.6366994434593496	0.0
4	0.0	0.0	0.0	3.420798828450511	0.0
5	0.0	0.0	0.0	3.690872678689985	0.0
6	0.0	0.0	0.0	5.239323408312865	0.0
7	0.0	0.0	0.0	6.399671048137898	0.0
8	0.0	0.0	0.0	7.360571130375863	0.0
9	0.0	0.0	0.0	8.806292707049789	0.0
10	0.0	0.0	0.0	10.849127485239515	0.0
11	0.0	1.3660791615552537E-4	0.0	12.712322853684725	0.0
12	0.0	1.3660791615552537E-4	0.0	13.60054752452795	0.0
13	0.0	1.3660791615552537E-4	0.0	13.965973700243982	0.0
14	0.0	1.3660791615552537E-4	0.0	14.426615593520413	0.0
15	0.0	1.3660791615552537E-4	0.0	14.818953528719081	0.0
16	0.0	1.3660791615552537E-4	0.0	15.573165833813738	0.0
17	0.0	1.3660791615552537E-4	0.0	16.474504864607894	0.0
18	0.0	1.3660791615552537E-4	0.0	17.62132832073353	0.0
19	0.0	1.3660791615552537E-4	0.0	18.125274923431263	0.0
20	0.0	1.3660791615552537E-4	0.0	18.689602225069738	0.0
21	0.0	1.3660791615552537E-4	0.0	19.282617189100876	0.0
22	0.0	1.3660791615552537E-4	0.0	19.931504790839618	0.0
23	0.0	1.3660791615552537E-4	0.0	20.582578119236853	0.0
24	0.0	1.3660791615552537E-4	0.0	21.062071904942748	0.0
25	0.0	1.3660791615552537E-4	0.0	21.482277855037143	0.0
26	0.0	1.3660791615552537E-4	0.0	21.86368715694337	0.0
27	0.0	1.3660791615552537E-4	0.0	22.278018966643078	0.0
28	0.0	1.3660791615552537E-4	0.0	22.69317042383972	0.0
29	0.0	1.3660791615552537E-4	0.0	23.134004169273602	0.0
30	0.0	1.3660791615552537E-4	0.0	23.661447333550086	0.0
31	0.0	1.3660791615552537E-4	0.0	24.073866632423616	0.0
32	0.0	1.3660791615552537E-4	0.0	24.518798615342163	0.0
33	0.0	1.3660791615552537E-4	0.0	24.94938676706438	0.0
34	0.0	1.3660791615552537E-4	0.0	25.370275756739552	0.0
35	0.0	1.3660791615552537E-4	0.0	25.813978268412697	0.0
36	0.0	1.3660791615552537E-4	0.0	26.219840387310764	0.0
37	0.0	1.3660791615552537E-4	0.0	26.61654977582641	0.0
38	0.0	1.3660791615552537E-4	0.0	27.043722729644738	0.0
39	0.0	1.3660791615552537E-4	0.0	27.46215277682911	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	40	6.8084773E-9	45.000004	1
GTATTCG	20	7.031605E-4	45.000004	29
CGACGTA	20	7.031605E-4	45.000004	24
CGAGTAC	20	7.031605E-4	45.000004	26
CGAAACG	35	1.2111195E-7	45.000004	1
CGACGAT	20	7.031605E-4	45.000004	34
TTTCGCG	20	7.031605E-4	45.000004	1
ATAGCTA	20	7.031605E-4	45.000004	27
TGACGTA	30	2.1644173E-6	45.000004	18
TAGCCGC	20	7.031605E-4	45.000004	22
CCGTCTA	20	7.031605E-4	45.000004	29
CGTTAAC	20	7.031605E-4	45.000004	45
CCCGTCA	30	2.1644173E-6	45.000004	35
TGCGCTT	20	7.031605E-4	45.000004	38
CGTAGAT	20	7.031605E-4	45.000004	27
TCCGTTG	20	7.031605E-4	45.000004	40
GCGATGT	30	2.1644173E-6	45.000004	9
CGTCGTC	20	7.031605E-4	45.000004	19
CGTCGAA	20	7.031605E-4	45.000004	21
CGGTATC	20	7.031605E-4	45.000004	29
>>END_MODULE