SRR3548508_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548508_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	438764
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGCT	3064	0.6983252956031033	TruSeq Adapter, Index 20 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1295	0.2951472773518338	No Hit
AAACTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCT	938	0.21378235224403094	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTT	855	0.1948655769388555	No Hit
CCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	646	0.14723176924269082	TruSeq Adapter, Index 22 (95% over 21bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	557	0.12694751620461112	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCT	457	0.10415622065620697	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACCC	25	3.8868486E-5	45.000004	35
GCAAGCG	35	1.209628E-7	45.000004	1
ATTAGCG	25	3.8868486E-5	45.000004	1
CGAGTTT	25	3.8868486E-5	45.000004	32
ATAGGCG	25	3.8868486E-5	45.000004	1
CAACCGA	30	2.1624219E-6	45.000004	25
AAGCGTT	25	3.8868486E-5	45.000004	29
TGAGTAC	25	3.8868486E-5	45.000004	27
CACTGCG	30	2.1624219E-6	45.000004	1
GTTACGG	30	2.1624219E-6	45.000004	2
ACTAGCG	25	3.8868486E-5	45.000004	1
ATATATC	25	3.8868486E-5	45.000004	20
CTACGCA	25	3.8868486E-5	45.000004	42
ACTACCG	25	3.8868486E-5	45.000004	1
TCGTCGG	30	2.1624219E-6	45.000004	2
GCGATTG	25	3.8868486E-5	45.000004	1
CGTACTA	35	1.209628E-7	45.000004	12
ACGTCCG	25	3.8868486E-5	45.000004	1
CATCGGT	35	1.209628E-7	45.000004	27
ACGTACT	35	1.209628E-7	45.000004	11