SRR3548507_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548507_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	445013
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGCT	2993	0.6725646217076805	TruSeq Adapter, Index 20 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1238	0.27819412017177025	No Hit
AAACTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCT	1007	0.22628552424311202	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTT	870	0.19549990674429737	No Hit
CCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	648	0.14561372364402836	TruSeq Adapter, Index 22 (95% over 21bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	523	0.11752465658306611	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCT	485	0.10898558019653358	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGAAA	30	2.1624928E-6	45.000004	20
CACGTCG	30	2.1624928E-6	45.000004	1
CAATCAG	30	2.1624928E-6	45.000004	1
CGATCCA	30	2.1624928E-6	45.000004	45
TACGTAG	30	2.1624928E-6	45.000004	1
TATCACG	25	3.8869395E-5	45.0	1
AACGTCG	20	7.0286234E-4	45.0	2
TAATACG	25	3.8869395E-5	45.0	1
CGAACGG	55	1.8189894E-12	45.0	2
TCACCGT	20	7.0286234E-4	45.0	28
GTCGATA	20	7.0286234E-4	45.0	36
GATCGTA	25	3.8869395E-5	45.0	23
CTCCGTA	20	7.0286234E-4	45.0	43
CAACGTT	25	3.8869395E-5	45.0	45
TCTCTCG	20	7.0286234E-4	45.0	26
TCCGATC	25	3.8869395E-5	45.0	18
CCGATAG	25	3.8869395E-5	45.0	11
GGCACGC	20	7.0286234E-4	45.0	9
CGTGATC	25	3.8869395E-5	45.0	20
CGTGAGT	20	7.0286234E-4	45.0	19