##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548507_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	445013
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85089649066432	33.0	31.0	34.0	30.0	34.0
2	32.04344592180453	34.0	31.0	34.0	30.0	34.0
3	32.19202135667947	34.0	31.0	34.0	30.0	34.0
4	35.79561271243762	37.0	35.0	37.0	35.0	37.0
5	35.6209841060823	37.0	35.0	37.0	33.0	37.0
6	35.596998289937595	37.0	35.0	37.0	33.0	37.0
7	35.677847613440505	37.0	35.0	37.0	35.0	37.0
8	35.85479300604701	37.0	35.0	37.0	35.0	37.0
9	37.433142402581495	39.0	37.0	39.0	35.0	39.0
10	37.0049841240593	39.0	37.0	39.0	33.0	39.0
11	37.02719471116574	39.0	37.0	39.0	33.0	39.0
12	36.991321601840845	39.0	37.0	39.0	33.0	39.0
13	36.91752825198365	39.0	37.0	39.0	33.0	39.0
14	38.088705273778515	40.0	37.0	41.0	33.0	41.0
15	38.06606997997811	40.0	37.0	41.0	33.0	41.0
16	38.01509843532661	40.0	37.0	41.0	33.0	41.0
17	38.023828517369154	40.0	37.0	41.0	33.0	41.0
18	37.98350160557107	40.0	37.0	41.0	33.0	41.0
19	37.95926411138551	40.0	37.0	41.0	33.0	41.0
20	37.881598964524635	40.0	37.0	41.0	33.0	41.0
21	37.84613258489078	40.0	37.0	41.0	33.0	41.0
22	37.82832186924876	40.0	37.0	41.0	33.0	41.0
23	37.713484774602094	40.0	37.0	41.0	33.0	41.0
24	37.607285629858005	39.0	36.0	41.0	32.0	41.0
25	37.59738704262572	39.0	36.0	41.0	32.0	41.0
26	37.51331758847494	39.0	36.0	41.0	32.0	41.0
27	37.45166096271345	39.0	36.0	41.0	32.0	41.0
28	37.367733077460656	39.0	36.0	41.0	32.0	41.0
29	37.204895137894844	39.0	36.0	41.0	31.0	41.0
30	37.21486563313881	39.0	36.0	41.0	31.0	41.0
31	37.0839593450079	39.0	36.0	41.0	31.0	41.0
32	37.08104257628429	39.0	36.0	41.0	31.0	41.0
33	37.00204038983131	39.0	36.0	41.0	31.0	41.0
34	36.90472637877995	39.0	35.0	41.0	31.0	41.0
35	36.85013022091489	39.0	35.0	41.0	31.0	41.0
36	36.769826948875654	39.0	35.0	41.0	31.0	41.0
37	36.59266358510875	39.0	35.0	40.0	30.0	41.0
38	36.49248448921717	39.0	35.0	40.0	30.0	41.0
39	36.466168404069094	39.0	35.0	40.0	30.0	41.0
40	36.39797489062117	39.0	35.0	40.0	30.0	41.0
41	36.359764770916804	39.0	35.0	40.0	30.0	41.0
42	36.24278167154667	39.0	35.0	40.0	29.0	41.0
43	36.154089880520345	39.0	35.0	40.0	29.0	41.0
44	36.045513277140216	38.0	35.0	40.0	29.0	41.0
45	35.82443209524216	38.0	35.0	40.0	28.0	41.0
46	35.68015316406487	38.0	34.0	40.0	28.0	41.0
47	35.56403970221095	38.0	34.0	40.0	28.0	41.0
48	35.48661275063875	38.0	34.0	40.0	28.0	41.0
49	35.28943199412152	38.0	34.0	40.0	27.0	41.0
50	35.05305238273938	37.0	34.0	40.0	26.0	41.0
51	33.83240264891138	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	3.0
14	6.0
15	24.0
16	55.0
17	86.0
18	202.0
19	363.0
20	543.0
21	830.0
22	1199.0
23	1619.0
24	2146.0
25	2702.0
26	3275.0
27	4185.0
28	5147.0
29	6373.0
30	8385.0
31	10909.0
32	14028.0
33	18355.0
34	26991.0
35	33369.0
36	40256.0
37	59598.0
38	91590.0
39	112728.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.80728652870815	32.71522404963451	19.959192203373835	27.51829721828351
2	22.72607766514686	31.611660782943417	19.888407754380207	25.77385379752951
3	21.884753928536917	31.66738949199237	19.981438744486116	26.466417834984597
4	21.46566504798736	31.331444249943257	19.125508693004473	28.077382009064905
5	19.699199798657567	33.259028387934734	17.06197347043794	29.979798342969755
6	20.801414790129726	37.54339760860918	18.87428007721123	22.78090752404986
7	83.83844966326826	5.273104381220324	5.780730001146034	5.107715954365378
8	85.53907413940716	4.877610317001975	4.57649551810846	5.006820025482401
9	76.52675315103154	9.368490358708623	9.152541611143944	4.952214879115891
10	31.025161062710527	36.9566731758398	13.95801920393337	18.060146557516298
11	23.43167502971823	28.77781098529706	28.851741409801512	18.938772575183197
12	26.49023736385229	22.564284638875716	30.65461009004231	20.290867907229675
13	25.67430614386546	24.81253356643514	29.022522937532163	20.49063735216724
14	21.881832665562577	28.158053809663986	29.407230799999102	20.55288272477433
15	19.84324053454618	30.436638929649245	29.322738886279726	20.397381649524846
16	21.524090307474165	27.890645891243626	27.624810960578678	22.960452840703528
17	20.3598546559314	27.143701419958514	29.142968857089567	23.35347506702051
18	21.247469175057805	26.15249442151128	28.77758627276057	23.82245013067034
19	20.631082687472052	30.192825827560092	28.742081691995512	20.43400979297234
20	22.88337644068825	27.638518425304433	27.468411035183244	22.009694098824077
21	22.839557496073148	28.040753865617408	27.163925548242414	21.95576309006703
22	21.78318386204448	25.649587764851812	26.913820495131603	25.653407877972107
23	22.569453027214937	27.953565401460185	25.174320750180335	24.30266082114455
24	19.387074085476154	29.41509573877617	26.8407889207731	24.357041254974572
25	22.70383112403458	27.257181250884805	25.308698846999977	24.730288778080638
26	23.276173954468746	27.246619761669884	26.453833932941283	23.023372350920084
27	21.494428252657787	26.85854121115563	25.468469460442726	26.178561075743858
28	23.01708039989843	25.860143411540786	29.312402109601294	21.81037407895949
29	22.818209805106818	27.854017747796135	25.37791030823819	23.949862138858865
30	25.208701768262948	25.82868365643251	25.820818717655442	23.141795857649104
31	23.17033434978304	27.13651061879091	26.180358776035757	23.512796255390292
32	24.47186936111979	27.35920074245022	24.2579430263835	23.910986870046493
33	22.768323622006548	25.673856718792486	27.512904117407803	24.04491554179316
34	21.628132211867968	26.081934685054144	28.287937655753876	24.001995447324013
35	24.209180405965668	25.45903153391025	27.772896522124075	22.558891538000015
36	24.02064658785249	26.01497034918081	29.407230799999102	20.5571522629676
37	23.172132050074943	28.305689946136404	26.29136676906068	22.230811234727973
38	22.186992290112872	27.961430340237253	28.86971841272053	20.981858956929347
39	22.35103244174889	24.573664140148715	28.931963785327618	24.143339632774776
40	23.62717493646253	24.567147476590574	28.764328233107793	23.041349353839102
41	22.691696647064244	23.252354425601048	31.12560756652053	22.93034136081418
42	24.709615224723773	24.95769786500619	27.367290393763778	22.96539651650626
43	22.210137681371105	23.7208800641779	28.79331615031471	25.275666104136285
44	23.98132189396714	23.522683606995752	27.754020669059106	24.741973829978
45	24.38850101008285	24.003343722542937	29.41914056443295	22.18901470294126
46	21.416677715033046	24.76264738333487	30.68427214485869	23.136402756773396
47	22.25845087671596	25.086458148413644	28.960502277461558	23.69458869740884
48	22.29552844523643	24.447375694642627	30.031482226361927	23.22561363375901
49	22.791019588191805	23.209209618595413	29.221618244860263	24.77815254835252
50	21.826103956513627	22.205194005568377	29.19263032765335	26.77607171026464
51	22.49484846510102	22.02632282652417	30.763820382775336	24.715008325599477
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1558.0
1	1337.5
2	1117.0
3	759.0
4	401.0
5	364.5
6	328.0
7	382.5
8	437.0
9	488.5
10	540.0
11	528.0
12	516.0
13	596.5
14	677.0
15	707.0
16	737.0
17	779.0
18	821.0
19	933.0
20	1045.0
21	1068.0
22	1091.0
23	1698.5
24	2306.0
25	2685.5
26	3175.0
27	3285.0
28	3990.5
29	4696.0
30	5316.5
31	5937.0
32	7741.5
33	9546.0
34	9999.5
35	10453.0
36	12696.0
37	14939.0
38	16322.0
39	17705.0
40	18314.0
41	18923.0
42	22293.0
43	25663.0
44	28825.0
45	31987.0
46	36102.5
47	40218.0
48	43899.5
49	47581.0
50	44708.5
51	41836.0
52	36817.5
53	31799.0
54	28288.5
55	24778.0
56	23556.5
57	22335.0
58	20524.0
59	18713.0
60	17601.5
61	16490.0
62	14600.0
63	12710.0
64	11166.0
65	9622.0
66	8470.0
67	7318.0
68	6112.5
69	4907.0
70	4110.5
71	3314.0
72	2796.0
73	2278.0
74	1783.0
75	1008.5
76	729.0
77	654.5
78	580.0
79	388.5
80	197.0
81	196.0
82	195.0
83	182.0
84	169.0
85	104.5
86	40.0
87	28.0
88	16.0
89	25.5
90	35.0
91	42.0
92	49.0
93	45.0
94	41.0
95	21.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	445013.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.982469171900526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.35965113012354	21.087491145538607
2	10.277027667829081	5.751532197875921
3	3.7176726995211378	3.1208898511669934
4	1.9225005985626247	2.151852549289558
5	1.0874527010889012	1.5214805842060068
6	0.6927630118553841	1.1631131773605747
7	0.5388087879898916	1.0554040209633362
8	0.42200782187977587	0.9447056692841374
9	0.2955632704362422	0.7443531092995422
>10	4.230485745940644	29.640830550131103
>50	1.3006346803694777	24.745649870137825
>100	0.1505998050954132	6.43233203933578
>500	0.0024160396539371102	0.4599512907642619
>1k	0.0024160396539371102	1.1804139446463517
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGCT	2993	0.6725646217076805	TruSeq Adapter, Index 20 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1238	0.27819412017177025	No Hit
AAACTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCT	1007	0.22628552424311202	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTT	870	0.19549990674429737	No Hit
CCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	648	0.14561372364402836	TruSeq Adapter, Index 22 (95% over 21bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	523	0.11752465658306611	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCT	485	0.10898558019653358	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7806513517582633	0.0
2	0.0	0.0	0.0	1.1190684317087365	0.0
3	0.0	0.0	0.0	1.4651257378997917	0.0
4	0.0	0.0	0.0	3.0106985638621793	0.0
5	0.0	0.0	0.0	3.2747357942352244	0.0
6	0.0	0.0	0.0	4.740086244671504	0.0
7	0.0	0.0	0.0	5.816009869374603	0.0
8	0.0	0.0	0.0	6.714185877715932	0.0
9	0.0	0.0	0.0	7.836400284935496	0.0
10	0.0	0.0	0.0	9.574102329594865	0.0
11	0.0	0.0	0.0	11.030464278571637	0.0
12	0.0	0.0	0.0	11.786397251316254	0.0
13	0.0	0.0	0.0	12.09312986362196	0.0
14	0.0	0.0	0.0	12.457613597805008	0.0
15	0.0	0.0	0.0	12.747717482410627	0.0
16	0.0	0.0	0.0	13.388597636473541	0.0
17	0.0	0.0	0.0	14.211494945091491	0.0
18	0.0	0.0	0.0	15.36067485668958	0.0
19	2.247125364876981E-4	0.0	0.0	15.793246489428398	0.0
20	2.247125364876981E-4	0.0	0.0	16.286265794482407	0.0
21	2.247125364876981E-4	0.0	0.0	16.762881084372815	0.0
22	2.247125364876981E-4	0.0	0.0	17.308932548037923	0.0
23	2.247125364876981E-4	0.0	0.0	17.860377112578735	0.0
24	2.247125364876981E-4	0.0	0.0	18.270028066595806	0.0
25	2.247125364876981E-4	0.0	0.0	18.6464215652127	0.0
26	2.247125364876981E-4	0.0	0.0	19.010455874322773	0.0
27	2.247125364876981E-4	0.0	0.0	19.40010741259244	0.0
28	2.247125364876981E-4	0.0	0.0	19.784590562522894	0.0
29	2.247125364876981E-4	0.0	0.0	20.15986049845735	0.0
30	2.247125364876981E-4	0.0	0.0	20.696923460662948	0.0
31	2.247125364876981E-4	0.0	0.0	21.06837328347711	0.0
32	2.247125364876981E-4	0.0	0.0	21.43465471795206	0.0
33	2.247125364876981E-4	0.0	0.0	21.823632118612267	0.0
34	2.247125364876981E-4	0.0	0.0	22.17935206387229	0.0
35	2.247125364876981E-4	0.0	0.0	22.572599002725763	0.0
36	2.247125364876981E-4	0.0	0.0	22.955284452364314	0.0
37	2.247125364876981E-4	0.0	0.0	23.30920669733244	0.0
38	2.247125364876981E-4	0.0	0.0	23.6880720338507	0.0
39	2.247125364876981E-4	0.0	0.0	24.06558909515003	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGAAA	30	2.1624928E-6	45.000004	20
CACGTCG	30	2.1624928E-6	45.000004	1
CAATCAG	30	2.1624928E-6	45.000004	1
CGATCCA	30	2.1624928E-6	45.000004	45
TACGTAG	30	2.1624928E-6	45.000004	1
TATCACG	25	3.8869395E-5	45.0	1
AACGTCG	20	7.0286234E-4	45.0	2
TAATACG	25	3.8869395E-5	45.0	1
CGAACGG	55	1.8189894E-12	45.0	2
TCACCGT	20	7.0286234E-4	45.0	28
GTCGATA	20	7.0286234E-4	45.0	36
GATCGTA	25	3.8869395E-5	45.0	23
CTCCGTA	20	7.0286234E-4	45.0	43
CAACGTT	25	3.8869395E-5	45.0	45
TCTCTCG	20	7.0286234E-4	45.0	26
TCCGATC	25	3.8869395E-5	45.0	18
CCGATAG	25	3.8869395E-5	45.0	11
GGCACGC	20	7.0286234E-4	45.0	9
CGTGATC	25	3.8869395E-5	45.0	20
CGTGAGT	20	7.0286234E-4	45.0	19
>>END_MODULE