SRR3548504_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3548504_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	853453
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCT	6242	0.7313818101289702	RNA PCR Primer, Index 21 (96% over 27bp)
AATCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	1811	0.21219680521364384	RNA PCR Primer, Index 14 (95% over 23bp)
AATGATACGGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCC	1499	0.1756394318140542	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGT	1407	0.16485969350391877	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1317	0.15431429733096025	No Hit
AAACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	1213	0.14212850619776368	TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	1145	0.13416087353375056	RNA PCR Primer, Index 21 (96% over 26bp)
AAAAACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTT	984	0.11529633149101357	RNA PCR Primer, Index 14 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	20	7.032263E-4	45.000004	1
ACCGGAC	35	1.2114288E-7	45.000004	4
CGAACAG	40	6.8121153E-9	45.000004	1
CTCGTAA	35	1.2114288E-7	45.000004	33
CGTATAG	20	7.032263E-4	45.000004	1
CTCAACG	35	1.2114288E-7	45.000004	1
TTAGCCG	20	7.032263E-4	45.000004	1
CGCATTT	40	6.8121153E-9	45.000004	23
ATAATCG	20	7.032263E-4	45.000004	5
TAGCGTG	20	7.032263E-4	45.000004	36
ATTACCG	40	6.8121153E-9	45.000004	1
GCACGGT	20	7.032263E-4	45.000004	3
GCGCATC	20	7.032263E-4	45.000004	13
TAGCCCG	75	0.0	45.000004	1
GCGCAAA	30	2.1648411E-6	45.000004	9
CGGATCG	40	6.8121153E-9	45.000004	1
TACGAAC	20	7.032263E-4	45.000004	8
GAACGTA	20	7.032263E-4	45.000004	9
CTCGCTA	20	7.032263E-4	45.000004	12
ACTCGCG	20	7.032263E-4	45.000004	1