##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548502_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	661739
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.866527437554687	33.0	31.0	34.0	30.0	34.0
2	32.07293056628066	34.0	31.0	34.0	30.0	34.0
3	32.20252395581944	34.0	31.0	34.0	30.0	34.0
4	35.82387013611106	37.0	35.0	37.0	35.0	37.0
5	35.63737364731412	37.0	35.0	37.0	33.0	37.0
6	35.6308922399919	37.0	35.0	37.0	33.0	37.0
7	35.716007368464	37.0	35.0	37.0	35.0	37.0
8	35.910774489640175	37.0	35.0	37.0	35.0	37.0
9	37.50569484343525	39.0	37.0	39.0	35.0	39.0
10	37.03290118913952	39.0	37.0	39.0	33.0	39.0
11	37.07042806907255	39.0	37.0	39.0	33.0	39.0
12	37.01424126430511	39.0	37.0	39.0	33.0	39.0
13	36.989180024148496	39.0	37.0	39.0	33.0	39.0
14	38.134101209086964	40.0	37.0	41.0	33.0	41.0
15	38.13806954101239	40.0	37.0	41.0	33.0	41.0
16	38.039251124688136	40.0	37.0	41.0	33.0	41.0
17	38.03697681412158	40.0	37.0	41.0	33.0	41.0
18	37.99350196980985	40.0	37.0	41.0	33.0	41.0
19	37.992895990715375	40.0	37.0	41.0	33.0	41.0
20	37.96521891561477	40.0	37.0	41.0	33.0	41.0
21	37.94555104051597	40.0	37.0	41.0	33.0	41.0
22	37.92694249545516	40.0	37.0	41.0	33.0	41.0
23	37.82308886131843	40.0	37.0	41.0	33.0	41.0
24	37.73517806869476	40.0	37.0	41.0	33.0	41.0
25	37.75317156764223	40.0	37.0	41.0	33.0	41.0
26	37.707153424537466	40.0	37.0	41.0	33.0	41.0
27	37.666785545358515	40.0	37.0	41.0	33.0	41.0
28	37.59833106406	40.0	36.0	41.0	32.0	41.0
29	37.4406571170809	39.0	36.0	41.0	32.0	41.0
30	37.44503950953472	39.0	36.0	41.0	32.0	41.0
31	37.36921505306473	39.0	36.0	41.0	32.0	41.0
32	37.37878075797256	39.0	36.0	41.0	32.0	41.0
33	37.36398187200694	39.0	36.0	41.0	32.0	41.0
34	37.30673120369209	39.0	36.0	41.0	32.0	41.0
35	37.24291148020594	39.0	36.0	41.0	31.0	41.0
36	37.21273190789722	39.0	36.0	41.0	31.0	41.0
37	37.134956531200366	39.0	36.0	41.0	31.0	41.0
38	37.12308629232975	39.0	35.0	41.0	31.0	41.0
39	37.032091202120476	39.0	35.0	41.0	31.0	41.0
40	36.93204873824877	39.0	35.0	41.0	31.0	41.0
41	36.895895511674546	39.0	35.0	41.0	31.0	41.0
42	36.78755068085756	39.0	35.0	41.0	31.0	41.0
43	36.69707543306349	39.0	35.0	40.0	31.0	41.0
44	36.588132179001086	39.0	35.0	40.0	30.0	41.0
45	36.41936020092514	39.0	35.0	40.0	30.0	41.0
46	36.285097296668326	38.0	35.0	40.0	30.0	41.0
47	36.213943866086176	38.0	35.0	40.0	30.0	41.0
48	36.16601258199985	38.0	35.0	40.0	30.0	41.0
49	36.024715182269745	38.0	35.0	40.0	29.0	41.0
50	35.841587090983	38.0	35.0	40.0	29.0	41.0
51	34.58912199522773	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	1.0
10	4.0
11	1.0
12	7.0
13	11.0
14	8.0
15	15.0
16	31.0
17	80.0
18	171.0
19	280.0
20	536.0
21	795.0
22	1241.0
23	1699.0
24	2314.0
25	2783.0
26	3651.0
27	4773.0
28	6417.0
29	8488.0
30	11407.0
31	14580.0
32	19585.0
33	25973.0
34	39315.0
35	49005.0
36	59315.0
37	85775.0
38	140094.0
39	183346.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.672106374265383	32.02455953177915	21.50137743128333	26.80195666267214
2	22.660293559847613	31.394855071259215	20.838880585850312	25.105970783042864
3	21.021581016080358	30.02936202944061	22.392060918277448	26.556996036201586
4	21.425516706737852	30.721024452238723	19.611357347836535	28.242101493186887
5	17.887112592729157	34.42369272477517	17.86656068329054	29.822633999205124
6	20.270076268740393	37.1882267782313	19.43303931006031	23.108657642967998
7	83.94850537749777	4.63778015199346	6.303089284445982	5.110625186062783
8	85.87192231378232	3.280598544139003	4.747491080320187	6.099988061758488
9	77.4414081684773	8.082642854660222	9.248208130395822	5.227740846466658
10	29.21287698019914	40.10493563172187	13.814056599354126	16.868130788724862
11	22.2376193635255	25.655885477506995	32.57326529039395	19.533229868573564
12	25.270537175532954	20.951764970781532	34.04680697374645	19.73089087993907
13	26.897613711750402	21.767192201154835	29.98825821056338	21.346935876531383
14	20.792638789613427	26.056194360616498	31.436714475042276	21.7144523747278
15	19.444221966666618	28.003790013887652	31.22469735046597	21.327290668979764
16	22.364557627705185	26.047429575708854	28.7463788593388	22.841633937247163
17	21.032310321743164	25.421503039718075	30.373002044612758	23.173184593926006
18	21.194458842534594	25.35622050385424	29.63993356897508	23.80938708463609
19	21.431712502965674	29.82656304071545	29.31019631607023	19.43152814024865
20	21.777770389836476	27.506615145850553	28.610524693270307	22.105089771042664
21	22.614202880591893	27.348697900531782	27.724072481748845	22.31302673712748
22	20.074530895111213	25.841910481322692	28.724013546126194	25.3595450774399
23	21.37020789163099	26.763572949455906	26.98601714573268	24.880202013180423
24	19.307158864748793	28.252377447906195	27.559506089258758	24.880957598086255
25	21.02717234438351	26.024459794571577	26.907436315526212	26.040931545518703
26	23.06664712220377	26.035944685140215	26.50833636826604	24.38907182438998
27	19.930365295078573	24.195339854534794	28.204775598838815	27.669519251547815
28	21.179649378380297	24.908309771677352	31.40361985616686	22.50842099377549
29	23.105484186363505	26.16741645875489	26.801805545690975	23.92529380919063
30	23.079038714659404	23.603414639306433	27.812475915731127	25.505070730303036
31	23.995412088451793	24.838947077322025	27.014427138191945	24.151213696034237
32	22.556325076805205	24.02639106959088	26.826286496639913	26.590997356963996
33	21.801344638898417	22.542120080575575	29.806917833163833	25.849617447362178
34	20.81863091037403	22.61767857115872	31.102594829683603	25.461095688783647
35	21.06510270665625	22.61118054096857	31.2402623995261	25.08345435284908
36	22.256508986171287	23.00302687313276	31.202029803291026	23.53843433740493
37	19.87792770261387	25.151759228336246	28.242705961111557	26.72760710793833
38	20.202829212121394	24.284045522479406	30.307719508748914	25.20540575665028
39	20.036902766800807	21.682113340758214	29.942923116213493	28.338060776227486
40	20.9510093858757	22.14135784652257	30.767115131494442	26.140517636107287
41	19.740713483714877	21.768854487947664	33.57260188684663	24.91783014149083
42	21.914682374773136	23.512139982681994	29.914966474697728	24.658211167847142
43	20.670838502793398	23.034761439177682	30.175794384190745	26.118605673838175
44	21.759031884171858	21.094419401002508	29.5102751991344	27.636273515691233
45	23.52211370343897	21.909393280432315	30.176549969096577	24.39194304703214
46	20.302264185728813	23.02418325049604	31.833396550603787	24.840156013171356
47	21.641311755843315	22.45386776357446	29.559085984051116	26.345734496531108
48	20.853387816042275	21.882796691747046	32.75808135836032	24.505734133850353
49	21.410405008621225	21.072054087789898	31.43399436938128	26.083546534207596
50	20.398827936694076	20.616587506554698	30.66359999939553	28.320984557355693
51	21.31716583124162	19.38785533269159	33.485709622676005	25.809269213390778
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1155.0
1	926.5
2	698.0
3	501.0
4	304.0
5	299.5
6	295.0
7	302.5
8	310.0
9	350.5
10	391.0
11	450.5
12	510.0
13	541.5
14	573.0
15	596.0
16	619.0
17	664.5
18	710.0
19	1045.0
20	1380.0
21	1749.5
22	2119.0
23	2404.5
24	2690.0
25	3150.0
26	4703.0
27	5796.0
28	6692.5
29	7589.0
30	8901.5
31	10214.0
32	12039.0
33	13864.0
34	15968.5
35	18073.0
36	19288.5
37	20504.0
38	23105.5
39	25707.0
40	26033.0
41	26359.0
42	29778.5
43	33198.0
44	42924.0
45	52650.0
46	58451.0
47	64252.0
48	69337.0
49	74422.0
50	69051.0
51	63680.0
52	56642.5
53	49605.0
54	44739.5
55	39874.0
56	35579.5
57	31285.0
58	29600.5
59	27916.0
60	25232.5
61	22549.0
62	20847.0
63	19145.0
64	16140.5
65	13136.0
66	11452.0
67	9768.0
68	7743.0
69	5718.0
70	4818.0
71	3918.0
72	3170.5
73	2423.0
74	2309.0
75	1679.0
76	1163.0
77	874.5
78	586.0
79	508.0
80	430.0
81	316.5
82	203.0
83	138.5
84	74.0
85	52.5
86	31.0
87	25.0
88	19.0
89	18.0
90	17.0
91	13.0
92	9.0
93	5.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	661739.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.241749204314992
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.59439037780724	20.78434315894485
2	10.82887055377216	6.1165249269125
3	4.000492429043015	3.3894271162438114
4	1.9591601057225916	2.213204334276667
5	1.1739295711954572	1.6576912266615573
6	0.7899705768210065	1.3386090545620148
7	0.5646430654777682	1.116255549162512
8	0.4632338888594741	1.0466028249687036
9	0.356642800809059	0.9064994882376523
>10	5.127161897556545	35.11167748511159
>50	1.0489793728673888	19.29211190098669
>100	0.08553216424918458	4.162542122557778
>500	0.0037655669795238498	0.7126714514003627
>1k	0.0026896906996598927	1.3797659606945414
>5k	5.379381399319786E-4	0.7720733992787557
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	5082	0.767976498287089	TruSeq Adapter, Index 16 (95% over 23bp)
AATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	2689	0.4063535623561555	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGT	2212	0.3342707623398349	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCC	2167	0.3274704981873518	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1013	0.15308150192145242	No Hit
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1001	0.15126809814745693	TruSeq Adapter, Index 19 (95% over 21bp)
AAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	923	0.13948097361648626	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	898	0.135703049087329	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8613667926478566	0.0
2	0.0	0.0	0.0	1.2116559549913184	0.0
3	0.0	0.0	0.0	1.6195206871591368	0.0
4	0.0	0.0	0.0	3.498055880037296	0.0
5	0.0	0.0	0.0	3.781097985761758	0.0
6	0.0	0.0	0.0	5.325211299318916	0.0
7	0.0	0.0	0.0	6.4664467410867426	0.0
8	0.0	0.0	0.0	7.546630922463388	0.0
9	0.0	0.0	0.0	9.109784975647498	0.0
10	0.0	0.0	0.0	11.182656606305507	0.0
11	0.0	0.0	0.0	13.05363595012535	0.0
12	0.0	0.0	0.0	13.954142040895277	0.0
13	0.0	0.0	0.0	14.338734757963486	0.0
14	0.0	0.0	0.0	14.768511452400418	0.0
15	0.0	0.0	0.0	15.085252644924962	0.0
16	0.0	0.0	0.0	15.765127943192104	0.0
17	0.0	0.0	0.0	16.620903407536808	0.0
18	0.0	0.0	0.0	17.782539641762085	0.0
19	0.0	0.0	0.0	18.276238819232358	0.0
20	0.0	0.0	0.0	18.828571385395147	0.0
21	0.0	0.0	0.0	19.393748894957074	0.0
22	0.0	0.0	0.0	20.0131774007577	0.0
23	0.0	0.0	0.0	20.628072397123336	0.0
24	0.0	0.0	0.0	21.070542917978237	0.0
25	0.0	0.0	0.0	21.446370850138802	0.0
26	0.0	0.0	0.0	21.82325660116753	0.0
27	0.0	0.0	0.0	22.236108193713836	0.0
28	0.0	0.0	0.0	22.651377657958804	0.0
29	0.0	0.0	0.0	23.074958556167914	0.0
30	0.0	0.0	0.0	23.68456445819273	0.0
31	0.0	0.0	0.0	24.091975839417053	0.0
32	0.0	0.0	0.0	24.506489718756185	0.0
33	0.0	0.0	0.0	24.935964179230783	0.0
34	1.5111698116629062E-4	0.0	0.0	25.346095666116096	0.0
35	3.0223396233258125E-4	0.0	0.0	25.789019537914495	0.0
36	3.0223396233258125E-4	0.0	0.0	26.198395439893975	0.0
37	3.0223396233258125E-4	0.0	0.0	26.588277251303005	0.0
38	3.0223396233258125E-4	0.0	0.0	27.03135224008257	0.0
39	3.0223396233258125E-4	0.0	0.0	27.456444308103347	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCA	35	1.210883E-7	45.0	15
CTCGTCA	20	7.0311147E-4	45.0	27
CGAAACG	20	7.0311147E-4	45.0	1
GGCAACG	40	6.8066583E-9	45.0	1
CGACCAG	40	6.8066583E-9	45.0	1
ATTCGGT	20	7.0311147E-4	45.0	34
CACGAAC	20	7.0311147E-4	45.0	36
CGGATTA	20	7.0311147E-4	45.0	6
TATTCGG	20	7.0311147E-4	45.0	33
TCGTCAA	20	7.0311147E-4	45.0	28
GTCGTAA	20	7.0311147E-4	45.0	24
CGCAGAT	20	7.0311147E-4	45.0	14
CGTACAT	70	0.0	45.0	1
CTTTACG	20	7.0311147E-4	45.0	1
CACGTGT	45	3.8380676E-10	45.0	22
GCGCTAA	20	7.0311147E-4	45.0	29
TTCGATG	20	7.0311147E-4	45.0	1
TTATTCG	20	7.0311147E-4	45.0	32
CGTCAAA	20	7.0311147E-4	45.0	29
CGATACG	20	7.0311147E-4	45.0	1
>>END_MODULE