Basic Statistics
Measure | Value |
---|---|
Filename | SRR3548501_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 667477 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 48 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT | 5168 | 0.7742588883212456 | TruSeq Adapter, Index 16 (95% over 23bp) |
AATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT | 2646 | 0.39641815373413614 | No Hit |
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCC | 2304 | 0.3451804331834655 | No Hit |
AATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGT | 2190 | 0.3281011929999086 | No Hit |
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG | 1073 | 0.1607546027803205 | No Hit |
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC | 939 | 0.14067900466982383 | TruSeq Adapter, Index 19 (95% over 21bp) |
AAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT | 923 | 0.138281918328272 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 906 | 0.13573501409037317 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AACGTTA | 25 | 3.8890408E-5 | 45.000004 | 38 |
CGAGTTA | 25 | 3.8890408E-5 | 45.000004 | 14 |
ACGTTAT | 25 | 3.8890408E-5 | 45.000004 | 39 |
CTATCGG | 25 | 3.8890408E-5 | 45.000004 | 2 |
CGTTGAT | 25 | 3.8890408E-5 | 45.000004 | 25 |
GCTATCG | 25 | 3.8890408E-5 | 45.000004 | 1 |
GTAATCG | 25 | 3.8890408E-5 | 45.000004 | 1 |
TCAATCG | 25 | 3.8890408E-5 | 45.000004 | 28 |
CTACGCG | 50 | 2.1827873E-11 | 45.000004 | 1 |
AATTCGG | 25 | 3.8890408E-5 | 45.000004 | 2 |
GCGGCTA | 25 | 3.8890408E-5 | 45.000004 | 29 |
AATTACG | 25 | 3.8890408E-5 | 45.000004 | 1 |
TATAGCG | 30 | 2.164128E-6 | 45.000004 | 1 |
CGCGTAG | 25 | 3.8890408E-5 | 45.000004 | 1 |
AACTACG | 30 | 2.164128E-6 | 45.000004 | 1 |
CAATCGT | 25 | 3.8890408E-5 | 45.000004 | 29 |
AATACCG | 30 | 2.164128E-6 | 45.000004 | 1 |
AGTACGG | 50 | 2.1827873E-11 | 45.000004 | 2 |
AGTACCG | 30 | 2.164128E-6 | 45.000004 | 1 |
ATGATCG | 20 | 7.0311595E-4 | 45.0 | 9 |