FastQCFastQC Report
Sat 18 Jun 2016
SRR3548501_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548501_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667477
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT51680.7742588883212456TruSeq Adapter, Index 16 (95% over 23bp)
AATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT26460.39641815373413614No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCC23040.3451804331834655No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGT21900.3281011929999086No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG10730.1607546027803205No Hit
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC9390.14067900466982383TruSeq Adapter, Index 19 (95% over 21bp)
AAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT9230.138281918328272No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9060.13573501409037317No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA253.8890408E-545.00000438
CGAGTTA253.8890408E-545.00000414
ACGTTAT253.8890408E-545.00000439
CTATCGG253.8890408E-545.0000042
CGTTGAT253.8890408E-545.00000425
GCTATCG253.8890408E-545.0000041
GTAATCG253.8890408E-545.0000041
TCAATCG253.8890408E-545.00000428
CTACGCG502.1827873E-1145.0000041
AATTCGG253.8890408E-545.0000042
GCGGCTA253.8890408E-545.00000429
AATTACG253.8890408E-545.0000041
TATAGCG302.164128E-645.0000041
CGCGTAG253.8890408E-545.0000041
AACTACG302.164128E-645.0000041
CAATCGT253.8890408E-545.00000429
AATACCG302.164128E-645.0000041
AGTACGG502.1827873E-1145.0000042
AGTACCG302.164128E-645.0000041
ATGATCG207.0311595E-445.09