##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548501_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	667477
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84499990261837	33.0	31.0	34.0	30.0	34.0
2	32.04205538168356	33.0	31.0	34.0	30.0	34.0
3	32.17479403784699	34.0	31.0	34.0	30.0	34.0
4	35.77468287296791	37.0	35.0	37.0	35.0	37.0
5	35.61119409357926	37.0	35.0	37.0	33.0	37.0
6	35.60604185612388	37.0	35.0	37.0	33.0	37.0
7	35.72460024839807	37.0	35.0	37.0	35.0	37.0
8	35.91041039616346	37.0	35.0	37.0	35.0	37.0
9	37.50020599960748	39.0	37.0	39.0	35.0	39.0
10	37.02397236159448	39.0	37.0	39.0	33.0	39.0
11	37.06511834864722	39.0	37.0	39.0	33.0	39.0
12	37.00082549660887	39.0	37.0	39.0	33.0	39.0
13	36.963616723872136	39.0	37.0	39.0	33.0	39.0
14	38.10828987365857	40.0	37.0	41.0	33.0	41.0
15	38.1147560140649	40.0	37.0	41.0	33.0	41.0
16	38.04070552243748	40.0	37.0	41.0	33.0	41.0
17	38.0143420672173	40.0	37.0	41.0	33.0	41.0
18	37.94981999379754	40.0	37.0	41.0	33.0	41.0
19	37.960875056368984	40.0	37.0	41.0	33.0	41.0
20	37.925074571857905	40.0	37.0	41.0	33.0	41.0
21	37.93004702783767	40.0	37.0	41.0	33.0	41.0
22	37.90025873550699	40.0	37.0	41.0	33.0	41.0
23	37.798661227278245	40.0	37.0	41.0	33.0	41.0
24	37.72545870494414	40.0	37.0	41.0	33.0	41.0
25	37.75498181959828	40.0	37.0	41.0	33.0	41.0
26	37.69080582551908	40.0	37.0	41.0	33.0	41.0
27	37.63297312117122	40.0	37.0	41.0	33.0	41.0
28	37.568916981409096	40.0	36.0	41.0	32.0	41.0
29	37.41040365435813	39.0	36.0	41.0	32.0	41.0
30	37.43300668038	39.0	36.0	41.0	32.0	41.0
31	37.359679809191924	39.0	36.0	41.0	32.0	41.0
32	37.37574628039618	39.0	36.0	41.0	32.0	41.0
33	37.34352494542883	39.0	36.0	41.0	32.0	41.0
34	37.27888002133407	39.0	36.0	41.0	32.0	41.0
35	37.207688055168944	39.0	36.0	41.0	31.0	41.0
36	37.20328940173219	39.0	36.0	41.0	31.0	41.0
37	37.10176380609369	39.0	35.0	41.0	31.0	41.0
38	37.057419206953945	39.0	35.0	41.0	31.0	41.0
39	36.98966406333102	39.0	35.0	41.0	31.0	41.0
40	36.92012608674157	39.0	35.0	41.0	31.0	41.0
41	36.905638696164814	39.0	35.0	41.0	31.0	41.0
42	36.77753990025124	39.0	35.0	41.0	31.0	41.0
43	36.6827051718636	39.0	35.0	40.0	31.0	41.0
44	36.62028654170855	39.0	35.0	40.0	30.0	41.0
45	36.460156679556	39.0	35.0	40.0	30.0	41.0
46	36.307472766851895	38.0	35.0	40.0	30.0	41.0
47	36.2105810387474	38.0	35.0	40.0	30.0	41.0
48	36.18286322974424	38.0	35.0	40.0	30.0	41.0
49	36.04851852573197	38.0	35.0	40.0	29.0	41.0
50	35.84139678221122	38.0	35.0	40.0	29.0	41.0
51	34.633193353478845	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	3.0
12	9.0
13	4.0
14	16.0
15	20.0
16	37.0
17	73.0
18	149.0
19	300.0
20	505.0
21	835.0
22	1270.0
23	1632.0
24	2265.0
25	2888.0
26	3742.0
27	5019.0
28	6430.0
29	8504.0
30	11357.0
31	15026.0
32	19693.0
33	26511.0
34	40312.0
35	49640.0
36	60048.0
37	86874.0
38	141352.0
39	182908.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.713038801336975	32.00694555767465	21.510104468019122	26.76991117296926
2	22.62729652107863	31.447225896922294	20.836373388146708	25.089104193852375
3	21.16777057486625	29.99069630863685	22.2529015981075	26.5886315183894
4	21.52223971762323	30.727500722871348	19.563220904989983	28.187038654515433
5	17.85814342666489	34.42111113941005	17.721359687300087	29.99938574662498
6	20.294781692852336	37.098656582923454	19.469285083980424	23.137276640243783
7	84.02806388834372	4.712222293801884	6.198715461356721	5.060998356497677
8	85.99592195686144	3.269925405669409	4.703083402124717	6.0310692353444395
9	77.55533149456835	8.044771580144335	9.15207565204494	5.247821273242374
10	29.32056085827676	40.102655222576956	13.821749663284278	16.755034255862
11	22.395528235429836	25.70560483732024	32.44980725927635	19.44905966797358
12	25.31458012785459	20.86903368955035	33.898546316951744	19.917839865643312
13	26.815455813458737	21.655577645372052	30.107104814098463	21.421861727070745
14	20.843414829275016	25.927035688121087	31.52333338826656	21.706216094337332
15	19.43452733202792	27.883807232309127	31.23553320938399	21.44613222627896
16	22.431035076864074	25.93977020931058	28.818820723410692	22.81037399041465
17	21.143200439865346	25.331959003830846	30.377376299108434	23.14746425719538
18	21.16372549166488	25.40791667727877	29.626339184720972	23.80201864633538
19	21.444933683108182	29.69810195707118	29.38198619577903	19.474978164041605
20	21.920605504009878	27.595707417633864	28.49686206416101	21.986825014195247
21	22.678684059525647	27.325585750520244	27.747323128737023	22.24840706121709
22	20.1075093224186	25.842688212477732	28.67709299346644	25.372709471637222
23	21.246125334655726	26.88407240998566	27.008421263953664	24.861380991404946
24	19.26763019549737	28.29910244098299	27.633161891720615	24.80010547179903
25	21.032185378672224	26.070860868614197	26.916133439803918	25.980820312909657
26	22.93292502962649	26.07220922968132	26.58997987945652	24.404885861235666
27	19.897764267532814	24.235441820467223	28.242621094060173	27.624172817939797
28	21.237735532460295	24.836960674300386	31.462507322349687	22.462796470889636
29	23.128737020152005	26.26008087170045	26.67927134567933	23.93191076246822
30	23.025362671672582	23.649054574165103	27.882009417552965	25.443573336609347
31	24.002774627440346	24.92100851415105	26.99718492172764	24.079031936680963
32	22.702505105044818	24.018805142349475	26.861749543429962	26.41694020917575
33	21.92644840196741	22.533959971654454	29.754583303993996	25.78500832238414
34	21.002821070988215	22.66400190568364	30.95792064745302	25.37525637587512
35	21.1727145654457	22.529015981075005	31.193734016303182	25.10453543717611
36	22.305637497621642	23.147014803506337	31.043916119956194	23.50343157891583
37	19.896266088569345	25.20161743400896	28.26404505323779	26.638071424183906
38	20.249986141844587	24.285031544158077	30.218569328980628	25.246412985016715
39	20.036196003757432	21.765394163394394	29.807019567715443	28.39139026513273
40	20.935552835528416	22.143534533774197	30.617834022745356	26.303078607952035
41	19.846526546982144	21.81543334077429	33.47411221660072	24.863927895642846
42	21.811538075469265	23.460883296353284	30.057215454614916	24.670363173562535
43	20.598312750851342	23.091132728168912	30.234749661786097	26.075804859193653
44	21.861577252849163	21.109641231083618	29.455846418678096	27.572935097389124
45	23.432268078151008	22.058138332856416	30.230105306999345	24.279488281993235
46	20.423475265814403	23.164243861586243	31.603785598604894	24.80849527399446
47	21.685391406745104	22.46399501406041	29.58094436212783	26.269669217066653
48	20.797570552992838	21.85663326226971	32.746596511939735	24.599199672797713
49	21.481788885609543	21.112188135321517	31.447225896922294	25.95879708214665
50	20.560558640971898	20.694495840306107	30.537981083992406	28.206964434729585
51	21.386354885636507	19.47392943876718	33.29882527787474	25.840890397721573
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1195.0
1	942.5
2	690.0
3	499.5
4	309.0
5	293.0
6	277.0
7	311.5
8	346.0
9	365.0
10	384.0
11	463.0
12	542.0
13	584.5
14	627.0
15	646.5
16	666.0
17	648.5
18	631.0
19	974.5
20	1318.0
21	1762.0
22	2206.0
23	2442.5
24	2679.0
25	3196.5
26	4823.0
27	5932.0
28	6712.5
29	7493.0
30	8959.5
31	10426.0
32	12148.0
33	13870.0
34	15882.5
35	17895.0
36	19279.5
37	20664.0
38	23494.0
39	26324.0
40	26634.0
41	26944.0
42	29993.5
43	33043.0
44	42809.5
45	52576.0
46	58738.5
47	64901.0
48	70096.5
49	75292.0
50	69404.0
51	63516.0
52	56864.5
53	50213.0
54	45209.5
55	40206.0
56	35890.0
57	31574.0
58	29978.0
59	28382.0
60	25651.0
61	22920.0
62	21135.0
63	19350.0
64	16403.0
65	13456.0
66	11623.0
67	9790.0
68	7743.0
69	5696.0
70	4894.0
71	4092.0
72	3257.0
73	2422.0
74	2282.0
75	1706.5
76	1271.0
77	947.0
78	623.0
79	556.5
80	490.0
81	363.0
82	236.0
83	145.0
84	54.0
85	50.0
86	46.0
87	34.0
88	22.0
89	19.5
90	17.0
91	12.5
92	8.0
93	6.0
94	4.0
95	3.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	667477.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.138476173629485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.41884478358178	20.658944146382165
2	10.987735238371895	6.183562524141533
3	3.987555160146425	3.366111775944404
4	1.971038298854516	2.2184805683851594
5	1.1556042264534232	1.625847099610259
6	0.8016184324065393	1.3533792696368174
7	0.5860137569153918	1.1542673895467994
8	0.45605201271531626	1.0266086954980558
9	0.39301071628399564	0.9952850408524441
>10	5.07957127829421	34.70109461947521
>50	1.0720165668524084	19.73137510644592
>100	0.08345039143162102	4.042911118617788
>500	0.004814445659516597	0.9279774766848603
>1k	0.002139753626451821	1.2362496376338616
>5k	5.349384066129552E-4	0.7779055311447458
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	5168	0.7742588883212456	TruSeq Adapter, Index 16 (95% over 23bp)
AATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	2646	0.39641815373413614	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCC	2304	0.3451804331834655	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGT	2190	0.3281011929999086	No Hit
TGCAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGG	1073	0.1607546027803205	No Hit
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	939	0.14067900466982383	TruSeq Adapter, Index 19 (95% over 21bp)
AAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	923	0.138281918328272	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	906	0.13573501409037317	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.857707456586519	0.0
2	0.0	0.0	0.0	1.1894042790987556	0.0
3	0.0	0.0	0.0	1.5919649665831181	0.0
4	0.0	0.0	0.0	3.3956226207045335	0.0
5	0.0	0.0	0.0	3.682224256416326	0.0
6	0.0	0.0	0.0	5.214561700253342	0.0
7	0.0	0.0	0.0	6.3530278945941205	0.0
8	0.0	0.0	0.0	7.424825125060489	0.0
9	0.0	0.0	0.0	8.981582886002064	0.0
10	0.0	0.0	0.0	11.04502477238916	0.0
11	0.0	0.0	0.0	12.938273528526077	0.0
12	0.0	0.0	0.0	13.846769251974225	0.0
13	0.0	0.0	0.0	14.23524705720197	0.0
14	0.0	0.0	0.0	14.665973509199567	0.0
15	0.0	0.0	0.0	14.991078344272537	0.0
16	0.0	0.0	0.0	15.652823992437193	0.0
17	0.0	0.0	0.0	16.491804211980337	0.0
18	0.0	0.0	0.0	17.65304272656586	0.0
19	0.0	0.0	0.0	18.134407627528738	0.0
20	0.0	0.0	0.0	18.67045606065827	0.0
21	0.0	0.0	0.0	19.250550955313816	0.0
22	0.0	0.0	0.0	19.864055240854743	0.0
23	0.0	0.0	0.0	20.478158797981052	0.0
24	0.0	0.0	0.0	20.913529604765408	0.0
25	0.0	0.0	0.0	21.317288835420545	0.0
26	0.0	0.0	0.0	21.692582665769756	0.0
27	0.0	0.0	0.0	22.105930241791103	0.0
28	0.0	0.0	0.0	22.516131641989162	0.0
29	0.0	0.0	0.0	22.950004269810044	0.0
30	0.0	0.0	0.0	23.529350074983856	0.0
31	0.0	0.0	0.0	23.957529622743554	0.0
32	0.0	0.0	0.0	24.35155069013614	0.0
33	0.0	0.0	0.0	24.77913096631045	0.0
34	0.0	0.0	0.0	25.204913427728595	0.0
35	0.0	0.0	0.0	25.64118314189103	0.0
36	0.0	0.0	0.0	26.038650020899595	0.0
37	0.0	0.0	0.0	26.435517628322774	0.0
38	0.0	0.0	0.0	26.85021356541124	0.0
39	0.0	0.0	0.0	27.27449784786592	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTA	25	3.8890408E-5	45.000004	38
CGAGTTA	25	3.8890408E-5	45.000004	14
ACGTTAT	25	3.8890408E-5	45.000004	39
CTATCGG	25	3.8890408E-5	45.000004	2
CGTTGAT	25	3.8890408E-5	45.000004	25
GCTATCG	25	3.8890408E-5	45.000004	1
GTAATCG	25	3.8890408E-5	45.000004	1
TCAATCG	25	3.8890408E-5	45.000004	28
CTACGCG	50	2.1827873E-11	45.000004	1
AATTCGG	25	3.8890408E-5	45.000004	2
GCGGCTA	25	3.8890408E-5	45.000004	29
AATTACG	25	3.8890408E-5	45.000004	1
TATAGCG	30	2.164128E-6	45.000004	1
CGCGTAG	25	3.8890408E-5	45.000004	1
AACTACG	30	2.164128E-6	45.000004	1
CAATCGT	25	3.8890408E-5	45.000004	29
AATACCG	30	2.164128E-6	45.000004	1
AGTACGG	50	2.1827873E-11	45.000004	2
AGTACCG	30	2.164128E-6	45.000004	1
ATGATCG	20	7.0311595E-4	45.0	9
>>END_MODULE