##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548497_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	443758
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.955728122084558	33.0	31.0	34.0	30.0	34.0
2	32.14370219804488	34.0	31.0	34.0	30.0	34.0
3	32.28511936686212	34.0	31.0	34.0	30.0	34.0
4	35.85696483218331	37.0	35.0	37.0	35.0	37.0
5	35.70098792585148	37.0	35.0	37.0	33.0	37.0
6	35.704237444733394	37.0	35.0	37.0	33.0	37.0
7	35.77220917707399	37.0	35.0	37.0	35.0	37.0
8	35.91189341938624	37.0	35.0	37.0	35.0	37.0
9	37.52905863105566	39.0	37.0	39.0	35.0	39.0
10	37.09717683962881	39.0	37.0	39.0	33.0	39.0
11	37.079347301907795	39.0	37.0	39.0	33.0	39.0
12	36.9832791746853	39.0	37.0	39.0	33.0	39.0
13	36.91622010194746	39.0	37.0	39.0	33.0	39.0
14	38.0868153362869	40.0	37.0	41.0	33.0	41.0
15	38.084933680068865	40.0	37.0	41.0	33.0	41.0
16	38.053319602125484	40.0	37.0	41.0	33.0	41.0
17	38.01807291361508	40.0	37.0	41.0	33.0	41.0
18	37.9818279332429	40.0	37.0	41.0	33.0	41.0
19	37.97302358492692	40.0	37.0	41.0	33.0	41.0
20	37.89654496369643	40.0	37.0	41.0	33.0	41.0
21	37.86062673799684	40.0	37.0	41.0	33.0	41.0
22	37.86543115842419	40.0	37.0	41.0	33.0	41.0
23	37.755172864489204	40.0	37.0	41.0	33.0	41.0
24	37.665989120196144	40.0	36.0	41.0	33.0	41.0
25	37.66732768761352	40.0	36.0	41.0	33.0	41.0
26	37.59295832413162	40.0	36.0	41.0	33.0	41.0
27	37.54338400659819	40.0	36.0	41.0	33.0	41.0
28	37.4896993406316	40.0	36.0	41.0	32.0	41.0
29	37.319448888808765	39.0	36.0	41.0	32.0	41.0
30	37.30654771294264	39.0	36.0	41.0	32.0	41.0
31	37.19098697938967	39.0	36.0	41.0	31.0	41.0
32	37.152831047552944	39.0	35.0	41.0	31.0	41.0
33	37.1145669486522	39.0	35.0	41.0	31.0	41.0
34	36.98688925044731	39.0	35.0	41.0	31.0	41.0
35	36.92334335380996	39.0	35.0	41.0	31.0	41.0
36	36.843376344764486	39.0	35.0	41.0	31.0	41.0
37	36.726459015950134	39.0	35.0	41.0	31.0	41.0
38	36.60992252534039	39.0	35.0	40.0	30.0	41.0
39	36.56687203385629	39.0	35.0	40.0	30.0	41.0
40	36.51049671217195	39.0	35.0	40.0	30.0	41.0
41	36.47451088205734	39.0	35.0	40.0	30.0	41.0
42	36.34393070096765	39.0	35.0	40.0	30.0	41.0
43	36.273137611040255	39.0	35.0	40.0	30.0	41.0
44	36.15795320873089	38.0	35.0	40.0	30.0	41.0
45	35.92768806421518	38.0	35.0	40.0	29.0	41.0
46	35.75129462454761	38.0	34.0	40.0	29.0	41.0
47	35.63544769897106	38.0	34.0	40.0	28.0	41.0
48	35.561091856372165	38.0	34.0	40.0	28.0	41.0
49	35.38751076036939	37.0	34.0	40.0	28.0	41.0
50	35.19053853677004	37.0	34.0	40.0	27.0	41.0
51	34.0085384376169	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	5.0
12	2.0
13	3.0
14	13.0
15	25.0
16	43.0
17	80.0
18	169.0
19	276.0
20	509.0
21	756.0
22	1088.0
23	1455.0
24	2014.0
25	2468.0
26	2965.0
27	3831.0
28	4644.0
29	6190.0
30	7959.0
31	10318.0
32	13660.0
33	18228.0
34	27595.0
35	33901.0
36	40861.0
37	58277.0
38	91950.0
39	114444.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.106738357392995	32.67839678383263	16.438464207969208	27.776400650805165
2	24.822087714475007	30.062105922597453	18.667381771145532	26.448424591782004
3	22.54832588933608	30.586941531194935	19.32584877343056	27.538883806038427
4	21.712284623601153	31.35470233776067	18.509412788051144	28.423600250587032
5	19.35153845113778	32.01745095299691	17.568133982936647	31.06287661292867
6	21.614032873773542	35.59507659580222	19.09982467921705	23.691065851207192
7	85.86459286367794	4.70323915287161	5.325199771046381	4.106968212404058
8	87.08868347162192	4.1806570247747645	4.297612662757629	4.4330468408456865
9	79.30831669513564	7.951631294534408	8.286498496928505	4.453553513401449
10	32.72887474704682	35.66200496667102	13.760202632966617	17.848917653315546
11	26.153894690349244	27.673641939976296	26.586112250370697	19.586351119303767
12	25.76764813254071	25.077407055196748	30.240581578247607	18.914363234014935
13	26.144430072246582	26.41124216352156	27.65899431672218	19.78533344750968
14	21.61899053087494	27.935045678049747	29.306739258785193	21.13922453229012
15	21.081310083423848	29.118348288932257	28.486021660454576	21.314319967189324
16	23.90807602341817	27.748682840647383	26.885599808904853	21.4576413270296
17	23.148202398604646	27.553080733192413	27.24480460070579	22.05391226749715
18	24.54152939214617	25.62072120389942	27.204241951694392	22.633507452260016
19	23.014120308816967	28.729848250623085	26.025446301813147	22.230585138746793
20	24.741187764502275	27.573362057698112	26.292258393088126	21.393191784711487
21	24.61611959671713	28.107887632448314	26.26138571022945	21.014607060605105
22	24.21657750395486	25.542074734427324	26.483128191491762	23.75821957012606
23	22.68466145962439	28.538527756119326	25.437287891147882	23.339522893108406
24	21.125252953186198	28.32174293195841	27.567277660346406	22.98572645450899
25	23.91145624416912	27.735387305693642	24.831326984527603	23.521829465609635
26	24.12981850468048	27.35883071403783	24.823890498875514	23.687460282406175
27	23.24374997183149	26.02747443426372	25.467484529856364	25.261291064048425
28	23.293326542845424	28.15881629176263	26.30803275659256	22.239824408799393
29	23.747853559823145	28.479035870902607	24.643161362724726	23.129949206549515
30	25.593003393741633	25.66128385291082	27.030273257045508	21.71543949630204
31	24.804285218520004	27.775499258604913	23.685882846055733	23.73433267681935
32	24.53612103894465	27.193650593341417	25.386584579883632	22.883643787830305
33	23.183582042464586	25.657903632159872	25.793112462197865	25.365401863177677
34	21.791607137223444	27.777527391055486	26.648984356338364	23.78188111538271
35	23.73388198071922	27.835216491871694	26.210006354815015	22.220895172594073
36	23.554955628968944	29.51451016094358	24.933860347306414	21.996673862781066
37	23.500872096953746	30.778262025698695	22.904150460386067	22.816715416961497
38	22.8345179129165	29.88070074229648	25.892265604225727	21.392515740561297
39	22.728378981336675	26.263413842680016	26.0804312260286	24.927775949954707
40	24.32925152898652	26.625548159131778	25.375091829330398	23.6701084825513
41	21.61538496207392	25.616890287048342	28.348784697966007	24.418940052911722
42	24.83538324942874	27.21505865809743	24.957521892563065	22.99203619991076
43	23.645770893144462	24.831552332577665	26.05316411197094	25.469512662306933
44	24.56338815300231	24.88203029579185	26.20910496261476	24.34547658859108
45	25.044280891837445	25.097237683602323	26.775404612423888	23.083076812136344
46	22.41807471639948	26.01620703176055	28.01864980462324	23.547068447216727
47	22.91654460313955	27.39398500984771	26.09440280513253	23.595067581880215
48	22.349343561130166	27.03545626219696	27.749584232847635	22.865615943825237
49	23.268988953438587	26.12572618409133	27.078497739759054	23.526787122711028
50	21.43803604667409	25.887758643224462	27.658318272571986	25.015887037529467
51	21.90766138300605	24.325420612135442	28.3469819135655	25.419936091293
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1213.0
1	985.5
2	758.0
3	525.5
4	293.0
5	281.0
6	269.0
7	305.0
8	341.0
9	378.5
10	416.0
11	457.0
12	498.0
13	579.5
14	661.0
15	792.0
16	923.0
17	799.5
18	676.0
19	712.5
20	749.0
21	1106.5
22	1464.0
23	1682.5
24	1901.0
25	2258.0
26	2956.0
27	3297.0
28	4257.5
29	5218.0
30	6305.5
31	7393.0
32	8386.5
33	9380.0
34	10094.5
35	10809.0
36	12035.0
37	13261.0
38	14647.5
39	16034.0
40	17336.0
41	18638.0
42	20594.0
43	22550.0
44	24898.0
45	27246.0
46	30318.5
47	33391.0
48	37758.0
49	42125.0
50	41606.0
51	41087.0
52	38424.5
53	35762.0
54	32667.0
55	29572.0
56	27058.5
57	24545.0
58	22734.0
59	20923.0
60	19597.5
61	18272.0
62	15961.0
63	13650.0
64	12649.5
65	11649.0
66	10221.0
67	8793.0
68	7038.0
69	5283.0
70	4795.0
71	4307.0
72	3652.5
73	2998.0
74	2349.0
75	1492.0
76	1284.0
77	1018.0
78	752.0
79	685.0
80	618.0
81	429.0
82	240.0
83	170.0
84	100.0
85	77.0
86	54.0
87	35.0
88	16.0
89	13.5
90	11.0
91	13.0
92	15.0
93	11.5
94	8.0
95	4.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	443758.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.755873662717523
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.09874583038008	20.36088429328902
2	9.384264530823604	5.0216839240847495
3	3.288219307437843	2.6393754109534635
4	1.4835610646999824	1.587758896721577
5	0.9256091946741043	1.238274133687502
6	0.6410952406071398	1.0291837958072476
7	0.5162236954337507	0.9668411183708622
8	0.37366785841205646	0.7998248009192962
9	0.32474453073401416	0.7819941273279993
>10	5.529089110974188	36.89972229184148
>50	1.3166907769077414	23.404067779067418
>100	0.11555838336730338	4.508932915711098
>500	0.00168698369879275	0.36380198509065964
>1k	8.43491849396375E-4	0.3976545271276317
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGCT	1762	0.3970632642115748	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	974	0.2194890007616764	No Hit
AAACTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCT	638	0.14377205594039993	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTT	487	0.1097445003808382	No Hit
CCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	457	0.10298405887893852	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4506961001266456	0.0
2	0.0	0.0	0.0	0.693170601994781	0.0
3	0.0	0.0	0.0	0.9433069375650692	0.0
4	0.0	0.0	0.0	2.1809184285128382	0.0
5	0.0	0.0	0.0	2.354887123161723	0.0
6	0.0	0.0	0.0	3.42686779731295	0.0
7	0.0	0.0	0.0	4.134009978411656	0.0
8	0.0	0.0	0.0	4.791575588496433	0.0
9	0.0	0.0	0.0	5.571054493665466	0.0
10	0.0	0.0	0.0	6.898579856588501	0.0
11	0.0	0.0	0.0	8.026446847155432	0.0
12	0.0	0.0	0.0	8.564352642656583	0.0
13	0.0	0.0	0.0	8.802320183523452	0.0
14	0.0	0.0	0.0	9.05245651909374	0.0
15	0.0	0.0	0.0	9.302818202714091	0.0
16	0.0	0.0	0.0	9.787767206450363	0.0
17	0.0	0.0	0.0	10.405446211673931	0.0
18	0.0	0.0	0.0	11.147291992482389	0.0
19	0.0	0.0	0.0	11.483511283176867	0.0
20	0.0	0.0	0.0	11.876292934437238	0.0
21	0.0	0.0	0.0	12.274257590849066	0.0
22	0.0	0.0	0.0	12.706475150870519	0.0
23	0.0	0.0	0.0	13.091144272328611	0.0
24	9.013922002532912E-4	0.0	0.0	13.408659674867833	0.0
25	9.013922002532912E-4	0.0	0.0	13.700260051649773	0.0
26	9.013922002532912E-4	0.0	0.0	13.998620869933612	0.0
27	9.013922002532912E-4	0.0	0.0	14.321544625674354	0.0
28	9.013922002532912E-4	0.0	0.0	14.615398482956927	0.0
29	9.013922002532912E-4	0.0	0.0	14.956575430752798	0.0
30	9.013922002532912E-4	0.0	0.0	15.407722226979569	0.0
31	9.013922002532912E-4	0.0	0.0	15.702702824512459	0.0
32	9.013922002532912E-4	0.0	0.0	16.02765471270377	0.0
33	9.013922002532912E-4	0.0	0.0	16.328945055638435	0.0
34	9.013922002532912E-4	0.0	0.0	16.64060140887601	0.0
35	9.013922002532912E-4	0.0	0.0	16.968257473668082	0.0
36	9.013922002532912E-4	0.0	0.0	17.288477052808062	0.0
37	9.013922002532912E-4	0.0	0.0	17.59495040089418	0.0
38	9.013922002532912E-4	0.0	0.0	17.932296431838974	0.0
39	0.001126740250316614	0.0	0.0	18.2878956548389	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	45	3.8380676E-10	45.000004	13
CCGTTGG	65	0.0	45.000004	2
ACGCGAA	25	3.886922E-5	45.0	1
ACAACCG	25	3.886922E-5	45.0	1
TTGGCGA	25	3.886922E-5	45.0	16
AGCCGAT	20	7.0286E-4	45.0	37
ACACGTA	20	7.0286E-4	45.0	11
TACCCGC	25	3.886922E-5	45.0	20
ACACGAT	25	3.886922E-5	45.0	12
TCACCGC	20	7.0286E-4	45.0	35
CTCCGTA	20	7.0286E-4	45.0	25
CGTATAC	20	7.0286E-4	45.0	14
CACGGCA	20	7.0286E-4	45.0	4
CCCTAGT	25	3.886922E-5	45.0	42
ACCGCGT	20	7.0286E-4	45.0	37
TTATGCG	20	7.0286E-4	45.0	1
CGACCGT	20	7.0286E-4	45.0	19
AAACGGT	20	7.0286E-4	45.0	3
AACGGAC	20	7.0286E-4	45.0	10
TACGCTT	20	7.0286E-4	45.0	8
>>END_MODULE